LOCUS       sequence-190763-        2666 bp    DNA     circular SYN 14-APR-2025
DEFINITION  chimeric spike resembling V4 region of prokaryotic 16S rRNA to be 
            used in metagenomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2666)
  TITLE     metagenomic_spikes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pSPIKE-P
FEATURES             Location/Qualifiers
     source          1..2666
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     278..300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     292..309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     320..339
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(809..826)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(919..1507)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(999..1018)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1678..2469,2470..2538))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2301..2320
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2539..2643)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattggcgg aaggccgtca
      361 aggccacgtg tcttgtccag agctcgtgcc agcagccgcg gtaatccctt gtctccctac
      421 ctctggagga gaaaagtgtt gacatgggcg ctcccggcgc aagggccaaa ggagtctccg
      481 atttcttatt cccgaatgac atgcgtctcc ctgcgggtaa atcaccgacc gcgattcata
      541 gaagcctggg ggaacaggta ggtctaacta gcttaagaga gtaaatcctg ggatcattca
      601 gtagtaacca caaacttacg ctggggcttc ttcggcggat cttttacgag actgattatt
      661 agaaacccta gtagtccggt acctggagca caagactggc ctcatgggcc ttccgctcac
      721 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaacatggt catagctgtt
      781 tccttgcgta ttgggcgctc tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
      841 gggtaaagcc tggggtgcct aatgagcaaa aggccagcaa aaggccagga accgtaaaaa
      901 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
      961 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     1021 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     1081 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     1141 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     1201 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     1261 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     1321 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     1381 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     1441 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     1501 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     1561 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     1621 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     1681 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     1741 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     1801 tgctgcaatg ataccgcgag aaccacgctc accggctcca gatttatcag caataaacca
     1861 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     1921 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     1981 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     2041 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     2101 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     2161 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     2221 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     2281 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     2341 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     2401 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     2461 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     2521 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     2581 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     2641 gcgcacattt ccccgaaaag tgccac
//
