LOCUS       sequence-190954-        9810 bp    DNA     circular SYN 14-APR-2025
DEFINITION  All-in-one lentivector with tet-inducible mouse Dux (codon sequences
            altered).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9810)
  AUTHORS   Whiddon JL, Langford AT, Wong CJ, Zhong JW, Tapscott SJ
  TITLE     Conservation and innovation in the DUX4-family gene network.
  JOURNAL   Nat Genet. 2017 Jun;49(6):935-940. doi: 10.1038/ng.3846. Epub 2017 
            May 1.
  PUBMED    28459454
REFERENCE   2  (bases 1 to 9810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ng.3846"; 
            journalName: "Nat Genet"; date: "2017-06"; volume: "49"; issue: "6";
            pages: "935-940"
COMMENT     Sequence Label: pCW57.1-mDux-CA
FEATURES             Location/Qualifiers
     source          1..9810
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     151..168
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    285..306
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(321..326,327..344,345..351)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    359..375
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     364..386
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     383..399
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     383..399
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     418..438
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        420..438
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        466..692
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             693..873
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    920..1045
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1538..1771
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1956..2000
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2213..2330
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2384..2698
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2392..2410
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2428..2446
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2463..2481
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2499..2517
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2535..2553
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2570..2588
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2606..2624
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2671..2695
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2718..2727
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(4755..4879)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             4897..4914
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        4924..5434
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5353..5371
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             5444..6040
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5444..5463)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5940..5960
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             6041..6094
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(6095..6712,6713..6718,6719..6754,6755..6757,6758..6793,
                     6794..6796,6797..6832,6833..6841)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(6881..6901)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7299..7310)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7515..7748
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7820..7941
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7857..7876)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7911..7930
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7981..8116
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        8116..8187
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8188..8256,8257..9048)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8406..8425)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9219..9807
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9708..9727
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
       61 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
      121 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
      181 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
      241 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
      301 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
      361 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca
      421 attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa
      481 tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga
      541 aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa
      601 ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata
      661 ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag
      721 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      781 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      841 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
      901 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
      961 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
     1021 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
     1081 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
     1141 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
     1201 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1261 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1321 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1381 agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
     1441 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
     1501 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
     1561 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
     1621 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
     1681 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
     1741 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
     1801 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
     1861 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
     1921 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
     1981 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
     2041 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
     2101 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
     2161 ccccgagggg acaattctcg acctcgagac aaatggcagt attcatccac aattttaaaa
     2221 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     2281 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     2341 acagggacag cagagatcca ctttggccgc gaatcgatat gtcgagttta ctccctatca
     2401 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacga tgtcgagttt
     2461 actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg
     2521 tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttat ccctatcagt
     2581 gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgaggtag
     2641 gcgtgtacgg tgggaggcct atataagcag agctcgttta gtgaaccgtc agatcgcctg
     2701 gagaattggc tagcgccgcc accatggctg aggctggctc tccagtggga ggatctggag
     2761 tggccagaga atcaaggaga aggaggaaaa ctgtctggca agcttggcag gaacaggcac
     2821 tcctgagcac atttaagaaa aaaaggtatc tgtcctttaa agaaagaaag gaactggcaa
     2881 aaaggatggg agtttctgat tgcaggatca gagtctggtt ccagaatagg agaaataggt
     2941 ctggggagga aggacatgca agcaagagaa gcataagagg ttccaggagg ctggcatccc
     3001 ctcaacttca ggaggaactg ggaagtaggc cccaaggcag gggcatgagg tcctcaggga
     3061 ggagacccag aaccaggctg acaagtctgc agctgagaat ccttggtcag gcttttgaaa
     3121 ggaatccaag gccaggattt gccaccagag aggaactggc cagggataca ggccttcctg
     3181 aggatactat ccatatctgg ttccagaaca ggagggccag gagaaggcac agaaggggaa
     3241 gacctacagc ccaggaccag gacctcctgg cttcccaggg ttctgatgga gcacctgctg
     3301 ggcctgaagg tagagagaga gaaggagcac aggaaaattt gctgccccag gaggaggcag
     3361 gatcaacagg gatggacacc tcaagccctt ctgacctccc ttcattctgt ggtgaatcac
     3421 agccctttca ggtggcccag cccaggggag ctggacagca ggaggctccc acaagggcag
     3481 ggaatgctgg atcattggag ccactgttgg accagctctt ggatgaggtc caggtggagg
     3541 aacctgcccc agctccactc aacctggatg gtgatcctgg ggggagggtt catgagggta
     3601 gtcaggagtc cttctggccc caggaggagg ctggttctac tggaatggac acttcttcac
     3661 cctctgacag caatagcttt tgcagggaga gtcaaccctc tcaggtagct cagccttgtg
     3721 gggctggcca ggaggatgct aggacccagg ctgactcaac agggcccttg gagctgttgc
     3781 tgctggacca gctcctggat gaggtacaga aggaggaaca tgtaccagtg cccctggact
     3841 gggggaggaa ccctggaagc agagaacatg agggtagtca ggattctctc cttcctctgg
     3901 aagaggctgt gaattctgga atggacacta gtataccaag tatttggcct acattttgca
     3961 gggagtcaca acccccacag gtggctcagc cttcaggacc tgggcaggcc caggctccta
     4021 cccaaggggg taatacagac ccactggaac tctttctgta tcagctgctg gatgaggtcc
     4081 aggtggagga acatgcccca gctccactca actgggatgt ggatccaggg ggcagagtcc
     4141 atgagggttc ctgggagtca ttctggcccc aggaggaggc aggctctaca ggactggaca
     4201 caagctcccc tagtgacagc aactcattct ttagggagag taagccctct caggttgctc
     4261 aaaggagggg agctgggcaa gaggatgcca ggactcaggc tgacagtaca ggacccctgg
     4321 agctgctgtt gtttgaccag ctcctggatg aagtgcagaa ggaggaacat gttccagctc
     4381 ccctggactg gggaaggaac cctggttcta tggaacatga gggctctcag gactctctct
     4441 tgcctctgga agaagctgct aatagtggca gagatacaag tatcccaagc atttggcctg
     4501 ccttttgcag gaaaagccag ccaccccagg tagcccagcc tagtggacct ggacaggctc
     4561 aggcacctat acaaggaggc aacactgacc cattggagtt gtttctggac cagctgctca
     4621 ctgaggtgca actggaggaa caagggccag cacctgtcaa tgttgaagag acctgggaac
     4681 agatggatac cactccagac ttgccactga cttctgaaga gtaccagacc cttcttgaca
     4741 tgctgtaagt cgaccatagt gactggatat gttgtgtttt acagtattat gtagtctgtt
     4801 ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt tctcgttcag
     4861 ctttcttgta caaagtggtt tagtaatgaa ccggtccacc accaccacca ccactaagga
     4921 tccggggttg gggttgcgcc ttttccaagg cagccctggg tttgcgcagg gacgcggctg
     4981 ctctgggcgt ggttccggga aacgcagcgg cgccgaccct gggtctcgca cattcttcac
     5041 gtccgttcgc agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc cggcgacgct
     5101 tcctgctccg cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca
     5161 aacggaagcc gcacgtctca ctagtaccct cgcagacgga cagcgccagg gagcaatggc
     5221 agcgcgccga ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc gcgccgagag
     5281 cagcggccgg gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt
     5341 cctgcccgcg cggtgttccg cattctgcaa gcctccggag cgcacgtcgg cagtcggctc
     5401 cctcgttgac cgaatcaccg acctctctcc ccagcaattc accatgaccg agtacaagcc
     5461 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
     5521 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     5581 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     5641 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     5701 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     5761 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     5821 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     5881 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     5941 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     6001 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc gaaggtagag gttctctcct
     6061 cacttgtggt gatgttgaag aaaaccctgg tccaatgtct agactggaca agagcaaagt
     6121 cataaacgga gctctggaat tactcaatgg tgtcggtatc gaaggcctga cgacaaggaa
     6181 actcgctcaa aagctgggag ttgagcagcc taccctgtac tggcacgtga agaacaagcg
     6241 ggccctgctc gatgccctgc caatcgagat gctggacagg catcataccc acttctgccc
     6301 cctggaaggc gagtcatggc aagactttct gcggaacaac gccaagtcat accgctgtgc
     6361 tctcctctca catcgcgacg gggctaaagt gcatctcggc acccgcccaa cagagaaaca
     6421 gtacgaaacc ctggaaaatc agctcgcgtt cctgtgtcag caaggcttct ccctggagaa
     6481 cgcactgtac gctctgtccg ccgtgggcca ctttacactg ggctgcgtat tggaggaaca
     6541 ggagcatcaa gtagcaaaag aggaaagaga gacacctacc accgattcta tgcccccact
     6601 tctgagacaa gcaattgagc tgttcgaccg gcagggagcc gaacctgcct tccttttcgg
     6661 cctggaacta atcatatgtg gcctggagaa acagctaaag tgcgaaagcg gcgggccgac
     6721 cgacgccctt gacgattttg acttagacat gctcccagcc gatgcccttg acgactttga
     6781 ccttgatatg ctgcctgctg acgctcttga cgattttgac cttgacatgc tccccgggta
     6841 actaagtaag gatcgatcca agatatcgta ttcttaacta tgttgctcct tttacgctat
     6901 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     6961 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     7021 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     7081 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     7141 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     7201 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca
     7261 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     7321 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     7381 agacgagtcg gatctccctt tgggccgcct ccccgcctgt ttcgcctcgg cgtccggact
     7441 agaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     7501 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc
     7561 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     7621 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     7681 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     7741 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     7801 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
     7861 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     7921 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
     7981 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     8041 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     8101 ggcttttttg gaggcctttc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     8161 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     8221 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     8281 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     8341 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     8401 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     8461 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     8521 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     8581 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     8641 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     8701 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     8761 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     8821 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     8881 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     8941 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     9001 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     9061 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     9121 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     9181 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     9241 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     9301 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     9361 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     9421 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     9481 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     9541 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     9601 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     9661 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     9721 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     9781 tcgtcagggg ggcggagcct atggaaaaac
//
