LOCUS       sequence-191026-        5459 bp    DNA     circular SYN 14-APR-2025
DEFINITION  to express Pak3 in mammalian system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5459)
  AUTHORS   Chan CP, Siu YT, Kok KH, Ching YP, Tang HM, Jin DY
  TITLE     Group I p21-activated kinases facilitate Tax-mediated 
            transcriptional activation of the human T-cell leukemia virus type 1
            long terminal repeats.
  JOURNAL   Retrovirology. 2013 Apr 26;10:47. doi: 10.1186/1742-4690-10-47.
  PUBMED    23622267
REFERENCE   2  (bases 1 to 5459)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Retrovirology."; date: "2013-04-26"; volume: "10:47. doi"; pages: "
            10.1186/1742-4690-10-47"
COMMENT     Sequence Label: Pak3
FEATURES             Location/Qualifiers
     source          1..5459
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        14..317
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        318..521
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     472..492
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     518..542
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          659..755
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     polyA_signal    2585..2719
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2609..2628)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2663..2682
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2830..2846)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2830..2846)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2843..2865)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2854..2870
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2878..2884,2885..2902,2903..2908))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2923..2944
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3061..3078)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3232..3820)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3312..3331)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3991..4782,4783..4851))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4614..4633
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4852..4956)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5024..5042
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5080..5102)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5202..5221
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5415..5437
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5429..5446
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5430..5446
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 catgcctgca ggtcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
       61 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      121 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      181 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      241 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      301 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      361 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      421 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      481 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga
      541 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca
      601 gcctccggac tctagaggat ccggtactag aggaactgaa aaaccagaaa gttaactggt
      661 aagtttagtc tttttgtctt ttatttcagg tcccggatcc ggtggtggtg caaatcaaag
      721 aactgctcct cagtggatgt tgcctttact tctaggcctg tacggaagtg ttacttctgc
      781 tctaaaagct gcggaattgt acccgcgggc ccaccatggc atcaatgcag aagctgatct
      841 cagaggagga cctgcttatg gccatggagg cccgaattcg gtcgaccgag atctctcgag
      901 gtaccccgct cgagatgtct gacagcttgg ataacgaaga aaaaccccca gctcccccac
      961 tgaggatgaa cagtaacaac cgagactctt cagcactcaa ccacagctcc aaaccactgc
     1021 ccatggcccc ggaagagaag aataagaaag ccaggcttcg ctctatcttc ccaggaggag
     1081 gggataaaac caataagaag aaagagaaag agcgcccaga gatctctctt ccttcagact
     1141 ttgagcatac gattcatgtg ggttttgatg cagtcaccgg ggaattcact ggaattcctg
     1201 agcaatgggc acgactactc caaacctcca acataacaaa gctggaacag aagaagaacc
     1261 cacaagctgt tcttgatgtt ctcaagttct acgactccaa agaaacggtc aacaaccaga
     1321 aatatatgag ctttacgtca ggagataaaa gtgcccatgg atacatagca gcacatcagt
     1381 cgaataccaa aacagcttcg gaacctcctt tggctcctcc tgtatctgaa gaagaggatg
     1441 aagaagagga agaggaagaa gatgacaatg aacctccgcc tgtcattgca ccaagaccag
     1501 agcatacaaa atcaatctat actcgctccg tggttgagtc aattgcttca ccagctgcac
     1561 caaataaaga agatatccca ccttctgctg agaatgccaa ttccaccact ttgtacagga
     1621 atacagatcg gcaaagaaaa aagtccaaga tgacggatga ggagatccta gagaagctga
     1681 gaagcattgt gagtgttggg gacccaaaga agaaatatac gagatttgaa aaaattggcc
     1741 aaggggcatc aggaactgtt tacacagcac tagacattgc gactggacaa gaggtggcca
     1801 taaagcaaat gaaccttcaa cagcagccca aaaaggaatt aattattaat gaaattcttg
     1861 tcatgaggga aaataagaac cccaatattg tcaattattt agatagctac ttagtgggtg
     1921 atgaactgtg ggtagtcatg gaatacttgg ctggtggctc tttgactgat gtggtcacag
     1981 aaacctgtat ggatgaagga cagatagcag ctgtctgtag agagtgcctc caagctttgg
     2041 atttcttgca ctcaaaccaa gtgatccaca gagacataaa gagcgacaac attctcctcg
     2101 ggatggatgg ttctgttaaa ctgactgatt ttgggttctg tgctcaaatc actcctgagc
     2161 aaagtaaacg aagcactatg gtgggaactc cctattggat ggcacctgaa gtggtaactc
     2221 gaaaagctta tggtccaaaa gttgatatct ggtctctggg aatcatggcc attgaaatgg
     2281 tggaaggtga acccccttac cttaatgaaa atccactcag ggccttatat ctgatagcca
     2341 ctaatgggac cccagagctc cagaatcctg agagactgtc agctgtattc cgtgacttct
     2401 taaatcgctg tcttgagatg gatgtggata gaagaggatc tgctaaggag cttttgcagc
     2461 atccgttttt aaaattagcc aagcctctgt ctagtctcac tcctctgatt atcgccgcaa
     2521 aggaagcaat taagaacagt agccgttagc tcgagcgacg cgtcggatat ctggcggccg
     2581 cggggatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     2641 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     2701 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     2761 ggaggtgtgg gaggtttttt cggatcctct agagtcgatc tgcaggcatg ctagcttggc
     2821 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     2881 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     2941 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     3001 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     3061 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     3121 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     3181 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     3241 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     3301 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     3361 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     3421 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     3481 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     3541 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     3601 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     3661 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     3721 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     3781 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     3841 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     3901 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     3961 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     4021 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     4081 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     4141 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     4201 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     4261 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     4321 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     4381 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     4441 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     4501 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     4561 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     4621 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     4681 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     4741 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     4801 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     4861 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     4921 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     4981 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     5041 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
     5101 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
     5161 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
     5221 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     5281 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     5341 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
     5401 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgagt tcgagcttg
//
