LOCUS       sequence-191154-        2881 bp    DNA     circular SYN 15-APR-2025
DEFINITION  CMYA5 (NM_153610, nt 11648 to 12279) T allele (rs10043986) in 
            pENTR3C, Gateway Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2881)
  TITLE     Shiang Lab CMYA5 project plasmids (will be published)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: CMYA5tnT/pENTR3C
FEATURES             Location/Qualifiers
     source          1..2881
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(54..140)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(305..893)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(385..404)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(983..1792)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1706..1725
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1784..1803
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    1915..2014
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2667..2766)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2785..2808)
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     terminator      complement(2816..2843)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc
       61 ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac
      121 tgagcctttc gttttatttg atgcctggca gttccctact ctcgcttagt agttagacgt
      181 ccccgagatc catgctagcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
      241 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
      301 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
      361 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
      421 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
      481 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
      541 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
      601 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
      661 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
      721 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
      781 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
      841 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
      901 ctttgatctt ttctacgggg tctgacgctc agtggaacgg ggcccaatct gaataatgtt
      961 acaaccaatt aaccaattct gattagaaaa actcatcgag catcaaatga aactgcaatt
     1021 tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt aatgaaggag
     1081 aaaactcacc gaggcagttc cataggatgg caagatcctg gtatcggtct gcgattccga
     1141 ctcgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg ttatcaagtg
     1201 agaaatcacc atgagtgacg actgaatccg gtgagaatgg caaaagttta tgcatttctt
     1261 tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc gcatcaacca
     1321 aaccgttatt cattcgtgat tgcgcctgag cgagacgaaa tacgcgatcg ctgttaaaag
     1381 gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa
     1441 tattttcacc tgaatcagga tattcttcta atacctggaa tgctgttttt ccggggatcg
     1501 cagtggtgag taaccatgca tcatcaggag tacggataaa atgcttgatg gtcggaagag
     1561 gcataaattc cgtcagccag tttagtctga ccatctcatc tgtaacatca ttggcaacgc
     1621 tacctttgcc atgtttcaga aacaactctg gcgcatcggg cttcccatac aagcgataga
     1681 ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat aaatcagcat
     1741 ccatgttgga atttaatcgc ggcctcgacg tttcccgttg aatatggctc ataacacccc
     1801 ttgtattact gtttatgtaa gcagacagtt ttattgttca tgatgatata tttttatctt
     1861 gtgcaatgta acatcagaga ttttgagaca cgggccagag ctgcagctgg atggcaaata
     1921 atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgataa gcaatgcttt
     1981 cttataatgc caactttgta caagaaagct gggtctagat aattccttgt gccttacaga
     2041 atccaggggc tctatcccca ggtgcaaagt acattttcca ggtttctcca gggcaaaggc
     2101 agggtggaca ccctcattaa acctgtgcct gatgatgaag agcaactgct cgctctctgc
     2161 gttgatgaag ataagtctct ggttgttgta gtccagcagg atgcccactc tggctggacg
     2221 ctcagtcaca tgaacatcac tcacaatacc actgtagaaa aatgtgtatc tctgtggctc
     2281 agagcagtgc atgtaccatg aggtctcccc ttgtcctagg gcacctgcct gcacagccga
     2341 gctggagcag atgccgagtc gatatgctgg gcagtctgtg actgtggttt cccagtaatg
     2401 ctggccacag gaaggcagct cctcacccag cactgacggg atttccgtca gccgtctcct
     2461 ctcaccaaag ctgatcactg tcccacttga ggaaatgtgt agagcaggat gagctgtttc
     2521 tctcaacaag agaaatctgg ttcctctggt ggagatgaga gcagcttcac tctgttcact
     2581 tgtcccaaat gcattgatgg ccctcacata gaaaaagtag ttatcattgg gttggaggtt
     2641 aactttcagc tggagtccca tgaaagagcc tgcttttttg tacaaagttg gcattataaa
     2701 aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat
     2761 tatttggggc ccgagcttaa gtaactaact aacaggaaga gtttgtagaa acgcaaaaag
     2821 gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc
     2881 t
//
