LOCUS       sequence-191218-       10044 bp    DNA     circular SYN 14-APR-2025
DEFINITION  For fusion gene cloning. Recombine Gene A + Gene B into this Gateway
            Destination Vector .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10044)
  AUTHORS   Lu H, Villafane N, Dogruluk T, Grzeskowiak CL, Kong K, Tsang YH, 
            Zagorodna O, Pantazi A, Yang L, Neill NJ, Kim YW, Creighton CJ, 
            Verhaak RG, Mills GB, Park P, Kucherlapati RS, Scott KL
  TITLE     Engineering and Functional Characterization of Fusion Genes 
            Identifies Novel Oncogenic Drivers of Cancer.
  JOURNAL   Cancer Res. 2017 May 16. pii: canres.2745.2016. doi: 
            10.1158/0008-5472.CAN-16-2745.
  PUBMED    28512244
REFERENCE   2  (bases 1 to 10044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2017 May 16. pii: canres.2745.2016. doi: 
            10.1158/0008-5472.CAN-16-2745."
COMMENT     Sequence Label: pFUSE-DEST_R1R4
FEATURES             Location/Qualifiers
     source          1..10044
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             32..665
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    712..837
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1334..1567
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1752..1796
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2089..2206
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2254..3432
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2485..3423
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3380..3400
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3452..3576
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3601..3606,3607..3624,3625..3631)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3685..4344
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3751..3770)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4686..4991
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4870..4889
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5032..5155)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             5209..5250
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5271..5838
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5288..5840
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5432..5449)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5659..5678
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5852..6451
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5852..5871)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6348..6368
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6465..7053
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6518..6538)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6936..6947)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7128..7361
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7480..7498)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7566..7670
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7671..7739,7740..8531)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7889..7908)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8702..9290
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9191..9210
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9444..9461
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9701..9722
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9737..9742,9743..9760,9761..9767)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9775..9791
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9780..9802
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9799..9815
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9799..9815
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9865..9884
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tttgttaaat atgtactaca aacttagtag ttggaagggc taattcactc ccaaagaaga
       61 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac
      121 tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta
      181 ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac
      241 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac
      301 agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc
      361 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg
      421 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg
      481 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
      541 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
      601 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
      661 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
      721 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
      781 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
      841 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
      901 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
      961 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
     1021 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
     1081 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
     1141 gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg
     1201 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     1261 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     1321 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1381 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1441 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1501 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     1561 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     1621 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     1681 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     1741 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     1801 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     1861 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     1921 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     1981 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     2041 gatctcgacg gtatcgccga attcacaaat ggcagtattc atccacaatt ttaaaagaaa
     2101 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     2161 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     2221 ggacagcaga gatccagttt ggactagtcg tgaggctccg gtgcccgtca gtgggcagag
     2281 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc
     2341 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
     2401 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
     2461 aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc
     2521 tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca gtacgtgatt
     2581 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga
     2641 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga
     2701 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat
     2761 ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa
     2821 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc
     2881 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg
     2941 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg
     3001 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt
     3061 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt
     3121 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc
     3181 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg
     3241 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag
     3301 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag
     3361 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt
     3421 caggtgtcgt gaagcggccc tgcagatatc aacaagtttg tacaaaaaag ctgaacgaga
     3481 aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac
     3541 ataatactgt aaaacacaac atatccagtc actatggcgg ccgcattagg caccccaggc
     3601 tttacacttt atgcttccgg ctcgtataat gtgtggattt tgagttagga tccgtcgaga
     3661 ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat
     3721 atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc
     3781 tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag
     3841 cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa
     3901 ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac
     3961 accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat
     4021 ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc
     4081 tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt
     4141 ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc
     4201 atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat
     4261 catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc
     4321 gatgagtggc agggcggggc gtaaagatct ggatccggct tactaaaagc cagataacag
     4381 tatgcgtatt tgcgcgctga tttttgcggt ataagaatat atactgatat gtatacccga
     4441 agtatgtcaa aaagaggtat gctatgaagc agcgtattac agtgacagtt gacagcgaca
     4501 gctatcagtt gctcaaggca tatatgatgt caatatctcc ggtctggtaa gcacaaccat
     4561 gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc aggaagggat
     4621 ggctgaggtc gcccggttta ttgaaatgaa cggctctttt gctgacgaga acaggggctg
     4681 gtgaaatgca gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg
     4741 atgtacagag tgatattatt gacacgcccg ggcgacggat ggtgatcccc ctggccagtg
     4801 cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg
     4861 aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag
     4921 aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct
     4981 ggggaatata aatgtcaggc tcccttatac acagccagtc tgcaggtcga ccatagtgac
     5041 tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat
     5101 atattgatat ttatatcatt ttacgtttct cgttcaactt ttctatacaa agtggttgat
     5161 atccagcaca gtggcggccg ctcgagtcta gagggcccgc ggttcgaagg taagcctatc
     5221 cctaaccctc tcctcggtct cgattctacg cgtaccggtt agtaatgaga tccctccccc
     5281 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg
     5341 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc
     5401 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg
     5461 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg
     5521 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca
     5581 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata
     5641 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc
     5701 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt
     5761 gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt
     5821 tgaaaaacac gatgataata tggccacaca tatgaccgag tacaagccca cggtgcgcct
     5881 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
     5941 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
     6001 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     6061 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     6121 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     6181 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     6241 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     6301 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     6361 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     6421 ctggtgcatg acccgcaagc ccggtgccta aatcgataga tcctaatcaa cctctggatt
     6481 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     6541 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     6601 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     6661 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     6721 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     6781 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     6841 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
     6901 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     6961 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     7021 cgagtcggat ctccctttgg gccgcctccc cgcctgagat cctttaagac caatgactta
     7081 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     7141 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     7201 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     7261 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     7321 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     7381 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggcccgggtt
     7441 aattaaggaa agggctagat cattcttgaa gacgaaaggg cctcgtgata cgcctatttt
     7501 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     7561 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     7621 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     7681 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     7741 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     7801 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     7861 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg
     7921 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     7981 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     8041 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     8101 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     8161 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     8221 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     8281 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     8341 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     8401 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     8461 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     8521 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     8581 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     8641 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     8701 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     8761 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     8821 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     8881 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     8941 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     9001 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     9061 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     9121 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     9181 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     9241 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     9301 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     9361 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     9421 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     9481 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagcaag ctcatggctg
     9541 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     9601 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctccccg tggcacgaca
     9661 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     9721 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     9781 gcggataaca atttcacaca ggaaacagct atgacatgat tacgaatttc acaaataaag
     9841 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     9901 tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc
     9961 ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat
    10021 tattttcatt ttaaagaaat tgta
//
