LOCUS       sequence-191357-        4388 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Donor Vector containing ZNF665 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 4388)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-ZNF665
FEATURES             Location/Qualifiers
     source          1..4388
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1843..1942)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1959..1978)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1960..1978)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1983..1999)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1983..1999)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2101..2120)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2112..2921
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2179..2198)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3068..3656
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3557..3576
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3810..3827
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3986..4013)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4105..4191)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4240..4262
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4254..4271
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4255..4271
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4287..4386
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atgggcgagg ccttctacac cgtgaagctg gagcggctgg agagctgcga caccgtgggc
       61 ctgagcttcc aggaggtgca gaagaacacc tacgacttcg agtgccagtg gaaggatgac
      121 gagggaaact acaagaccgt gctgatgctg cagaaggaga acctgcctgg caggagagcc
      181 cagagagata gaagggccgc cggcaataga cacatcgaga atcagctggg cgtgtccttc
      241 cagagccacc tccccgagct gcagcagttc cagcacgagg gcaagatcta cgagtacaac
      301 caggtggaga agagccccaa caacagagga aagcactata agtgcgacga atgcggcaag
      361 gtcttctctc agaactccag actgaccagc cacaagcgga tccacacggg cgagaaaccc
      421 taccagtgca acaagtgtgg taaagctttc accgtgagat ccaacctgac aatccaccaa
      481 gtgattcata caggtgaaaa accgtacaag tgtaatgagt gcggaaaagt ctttagccag
      541 cccagcaacc tggctggcca ccagagaatt cataccgggg agaagcctta taagtgtaac
      601 gagtgcggca aagcctttag agcccacagc aagctgacca ctcaccaggt gattcacaca
      661 ggcgagaagc catacaaatg taaggagtgc gggaagtgct ttacacagaa cagccatctc
      721 gccagccacc ggaggatcca tactggtgag aaaccttata aatgcaacga gtgtgggaaa
      781 gccttcagcg tgaggagcag cctgaccacc catcagacca tccataccgg tgagaagccg
      841 tataaatgta atgaatgcgg aaaggtgttc aggcacaaca gctacctggc taagcacagg
      901 agaatccaca ccggcgaaaa gccatataag tgcaatgaat gtggaaaggc attctccatg
      961 cactctaacc tgaccaagca ccagatcatt catactggag aaaaaccctt caaatgcaat
     1021 gagtgtgtca aagtgtttac tcagtactcc catctggcca atcacagaag aattcacacc
     1081 ggggaaaagc cgtaccgctg cgacgagtgc ggtaaagcct tctccgtgcg gagcagcttg
     1141 accacacacc aggctatcca cacaggggag aaaccgtata agtgcaacga ctgtggcaag
     1201 gtgtttacgc agaatagcca cctggcctcc cacagaggca tccactcagg agaaaagccc
     1261 tataaatgtg atgagtgtgg aaaagcattc agccagacct cccagctggc caggcactgg
     1321 agggtgcaca ccggagagaa accttacaaa tgcaacgaat gcgggaaggc cttttccgtg
     1381 cacagcagcc tcaccatcca ccagaccata cacacaggac agaagccgta caaatgcaat
     1441 gactgcggga aagtgttcag acacaactct tacctggcaa ttcaccagcg cattcacact
     1501 ggagagaagc cctacaaatg caacgaatgc ggtaaggctt ttagcgtgca ctccaatctg
     1561 gctacccacc aggttattca cacgggggaa aaaccatata aatgtaacga atgtggcaaa
     1621 gtcttcaccc agaattccca cctcgccaac cacagacgca tccacactgg cgaaaaacct
     1681 taccggtgca acgaatgtgg gaaggcattt agcgtcagaa gcaccctgac aacccacatg
     1741 gccgtgcata ccggcgacaa accatacaag tgcaaccaat gtggtaaggt ctttacccag
     1801 aactcaaacc tggccaagca cagaaggatc cactccggct atacccagct ttcttgtaca
     1861 aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca
     1921 gtcaaaataa aatcattatt tgccatccag ctgatatccc ctatagtgag tcgtattaca
     1981 tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg
     2041 cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata
     2101 caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta aattccaaca
     2161 tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga
     2221 caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag
     2281 gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta
     2341 tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca
     2401 ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa
     2461 atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt
     2521 gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg
     2581 gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct
     2641 ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt
     2701 tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac
     2761 gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt
     2821 tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga
     2881 ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt
     2941 tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcaagctca tgaccaaaat
     3001 cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     3061 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     3121 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     3181 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     3241 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     3301 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     3361 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     3421 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     3481 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     3541 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     3601 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     3661 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     3721 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     3781 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     3841 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     3901 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta
     3961 gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttagt
     4021 ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac
     4081 aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca
     4141 acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt gatgcctggc
     4201 agttccctac tctcgcgtta acgctagcat ggatgttttc ccagtcacga cgttgtaaaa
     4261 cgacggccag tcttaagctc gggccccaaa taatgatttt attttgactg atagtgacct
     4321 gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag
     4381 caggctta
//
