LOCUS       sequence-191369-        5066 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Donor Vector containing ZNF347 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5066)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 5066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-ZNF347
FEATURES             Location/Qualifiers
     source          1..5066
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(2521..2620)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2637..2656)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2638..2656)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2661..2677)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2661..2677)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2779..2798)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2790..3599
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2857..2876)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3746..4334
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4235..4254
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4488..4505
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4664..4691)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4783..4869)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4918..4940
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4932..4949
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4933..4949
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4965..5064
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atggccctca cccagggcca ggtgaccttc agggacgtgg ccatcgagtt ctcacaggag
       61 gagtggacct gcctggatcc tgcccagaga accctgtaca gagacgtgat gctcgagaac
      121 tacagaaacc tggcctctct gggaatcagc tgcttcgacc tgagcatcat tagcatgctg
      181 gagcagggca aggagccttt caccctggag agccaggttc agattgctgg caaccccgac
      241 ggctgggaat ggatcaaggc cgtgatcacc gccctgagct ccgagttcgt gatgaaggac
      301 ctgctgcaca agggcaagtc caacactggc gaggtgttcc agacagtgat gctggaaaga
      361 caggagtccc aggacatcga gggctgcagc ttcagagagg tgcagaagaa cacccacgga
      421 ctggagtacc aatgcaggga cgccgaggga aactacaagg gcgtgctcct gacacaggaa
      481 ggaaacctga cccacggcag agacgagcac gacaagagag acgccagaaa caagctgatc
      541 aagaaccagc tgggcctgag cctgcagagc cacctccccg agctccagct gttccagtac
      601 gagggcaaga tctacgagtg caaccaggtg gagaagagct tcaacaacaa cagcagcgtg
      661 tccccccctc agcagatgcc atacaacgtg aagacacaca tctctaagaa gtacctcaag
      721 gacttcatca gcagcctgct gctgacccag gggcagaagg ccaacaactg gggcagcccc
      781 tacaagagca acggctgcgg catggtgttc ccccaaaact ctcatctggc ctcccatcag
      841 agatcccaca ccaaggagaa accgtataaa tgctacgaat gtggtaaagc attcagaacc
      901 agatccaatc tgacaaccca ccaagttatt cacaccggcg aaaagagata caagtgcaat
      961 gagtgtggga aagtcttctc tagaaacagc cagctgtccc agcaccagaa gatccacacg
     1021 ggtgaaaagc cgtacaagtg caatgagtgt ggaaaagtgt tcacacagaa ctctcacctt
     1081 gtgagacata gaggcattca tacaggagag aaaccctata agtgtaatga gtgtggcaaa
     1141 gcctttagag ccagaagcag cctcgccatc catcaggcca cccacagcgg ggagaagcct
     1201 tataaatgta atgaatgcgg caaagtgttt acacagaatt cacatctgac caaccactgg
     1261 agaatacaca ccggggaaaa accctacaag tgtaacgagt gtgggaaggc attcggcgtg
     1321 agaagctccc tggccatcca cctcgtgatc cataccggag agaagccgta taagtgccac
     1381 gaatgtggga aagttttcag aagaaactcc catctcgcca gacatcagtt aattcacact
     1441 ggtgagaaac cttacaaatg caatgagtgc ggcaaggctt tcagagccca cagcaacctc
     1501 accacacacc aggttataca cacaggcgag aaaccataca agtgcaatga atgtggaaag
     1561 gtcttcactc agaacagcca cctggctaac caccagagaa tccacacagg tgtcaagcca
     1621 tacatgtgca acgaatgcgg gaaagcattt tccgtgtaca gctcccttac aacccatcag
     1681 gtcatccata ctggtgaaaa accttataag tgcaacgagt gcgggaaggt gttcacccag
     1741 aattcccacc tcgctagaca caggggcatt cacacaggag aaaagcccta caaatgtaat
     1801 gagtgcggaa aggttttcag gcacaacagc tacctgagcc ggcaccagcg aatccataca
     1861 ggggaaaagc cttacaagta caacgagtac ggaaaggcat ttagcgagca ctccaacctg
     1921 acaactcacc aggtgattca cacgggagaa aaaccgtaca aatgcaacga gtgtggtaag
     1981 gtctttaccc agaacagtca cttggccagg catagaagag tgcacaccgg aggcaagccc
     2041 taccagtgca atgagtgcgg taaagccttc tcccagacca gcaaactggc ccggcatcag
     2101 agggtgcata caggcgaaaa accatacgag tgtaaccagt gcggcaaggc cttcagcgtg
     2161 agatccagcc tgaccaccca ccaggccata cacactggca agaagcccta taaatgtaac
     2221 gaatgtggca aggtgtttac tcagaatagc catctggcta ggcacagagg catacatact
     2281 ggcgaaaagc catataaatg taatgagtgt gggaaagctt ttagccagac aagcaagctg
     2341 gccagacacc agcggattca taccggcgag aagccatacg aatgcggaaa acccttctcc
     2401 atctgcagct ctctgaccac acatcagacc atccacaccg gtggcaaacc atataagtgc
     2461 aatgtgtgga aggtgctgaa gagcgagttc aagccctgca agcccagcca gaactcctat
     2521 acccagcttt cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca
     2581 acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct gatatcccct
     2641 atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa
     2701 atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg
     2761 cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcgaggc
     2821 cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg
     2881 tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg ccagagttgt
     2941 ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa
     3001 actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg
     3061 atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta ttagaagaat
     3121 atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt
     3181 cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc
     3241 aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct
     3301 ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag
     3361 tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag
     3421 gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat
     3481 ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta
     3541 ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat
     3601 cagaattggt taattggttg taacactggc agagcattac gctgacttga cgggacggcg
     3661 caagctcatg accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc
     3721 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     3781 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     3841 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     3901 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     3961 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     4021 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     4081 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     4141 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     4201 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     4261 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     4321 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     4381 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     4441 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     4501 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     4561 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     4621 ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc catccgtcag
     4681 gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg cccgccaccc
     4741 tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc tactcaggag
     4801 agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact gagcctttcg
     4861 ttttatttga tgcctggcag ttccctactc tcgcgttaac gctagcatgg atgttttccc
     4921 agtcacgacg ttgtaaaacg acggccagtc ttaagctcgg gccccaaata atgattttat
     4981 tttgactgat agtgacctgt tcgttgcaac aaattgatga gcaatgcttt tttataatgc
     5041 caactttgta caaaaaagca ggctta
//
