LOCUS       sequence-192015-        2433 bp    DNA     circular SYN 15-APR-2025
DEFINITION  BB1 with insert: IDP1tt.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Prielhofer R, Barrero JJ, Steuer S, Gassler T, Zahrl R, Baumann K, 
            Sauer M, Mattanovich D, Gasser B, Marx H
  TITLE     GoldenPiCS: a Golden Gate-derived modular cloning system for applied
            synthetic biology in the yeast Pichia pastoris.
  JOURNAL   BMC Syst Biol. 2017 Dec 8;11(1):123. doi: 10.1186/s12918-017-0492-3.
  PUBMED    29221460
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Syst 
            Biol."; date: "2017-12-8"; volume: "11(1):123. doi"; pages: " 
            10.1186/s12918-017-0492-3"
COMMENT     Sequence Label: BB1_34_IDP1tt
FEATURES             Location/Qualifiers
     source          1..2433
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(729..745)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(729..745)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      793..824
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        825..927
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             928..1743
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1001..1020)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1767..1794
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1806..2393)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1886..1905)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(2433,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagttta tctagtcagc ttgattctag ctgatcgtgg accggaaggt
       61 gagccagtga gttgattgca gtccagttac gctggagtct gaggctcgtc ctgaatgata
      121 tgcggcctcg aattcgaaga cgcgcttatg gaccatccta tataattact acatctctat
      181 aatcataaag agtaccatga gaactggttt cttgaaggaa gtttcgtaaa aaggttacgc
      241 ttgagtagtt ttgtgtgacg tttccgtttc ctgtgactct aggaactccc agagatattg
      301 taaggagtag taagtagcca ttgtgatacc caagaccttg aaataggcca cactgtattt
      361 tctgtaaaag acggtctaat tgttgttagt aagcagcggg taatcgaata gtgaaaaagg
      421 tactaacatg aggattgtat ttctcttcag gttctgattc cttcttagag ggagcaggag
      481 taggttgcga gggaagtgga ggtggtttgg gatgatcttg gccccgaact tgactctggt
      541 gccttagtga gttcaataga ggtctagccc ctctgaaccg ctgagcaaac atccgaaaca
      601 gctgattcta aatagaattg acggagtttg aaggaaatga gacgctgcgt cttcgaattc
      661 gcgcgtgatc ttacggcatt atacgtatga tcggtccacg atcagctaga ttatctagtc
      721 agcttgatgt catagctgtt tcctgaggct caatactgac catttaaatc atacctgacc
      781 tccatagcag aaagtcaaaa gcctccgacc ggaggctttt gacttgatcg gcacgtaaga
      841 ggttccaact ttcaccataa tgaaataaga tcactaccgg gcgtattttt tgagttatcg
      901 agattttcag gagctaagga agctaaaatg agccatattc aacgggaaac gtcttgcttg
      961 aagccgcgat taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat
     1021 aatgtcgggc aatcaggtgc gacaatctat cgattgtatg ggaagcccga tgcgccagag
     1081 ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcagg
     1141 ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct
     1201 gatgatgcat ggttactcac cactgcgatc ccagggaaaa cagcattcca ggtattagaa
     1261 gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg
     1321 cattcgattc ctgtttgtaa ttgtcctttt aacggcgatc gcgtatttcg tctcgctcag
     1381 gcgcaatcac gaatgaataa cggtttggtt ggtgcgagtg attttgatga cgagcgtaat
     1441 ggctggcctg ttgaacaagt ctggaaagaa atgcataaac tcttgccatt ctcaccggat
     1501 tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta
     1561 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc
     1621 ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat
     1681 ggtattgata atcctgatat gaataaattg cagtttcact tgatgctcga tgagtttttc
     1741 taatgaggac ctaaatgtaa tcacctggct caccttcggg tgggcctttc tgcgttgctg
     1801 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgatg ctcaagtcag
     1861 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     1921 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     1981 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     2041 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     2101 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     2161 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     2221 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     2281 gttacctcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     2341 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     2401 ctttgatttt ctaccgaaga aaggcccacc cgt
//
