LOCUS       sequence-192091-        3765 bp    DNA     circular SYN 15-APR-2025
DEFINITION  dsRNA and riboprobe synthesis for Schmidtea mediterranea CRELD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Roberts-Galbraith RH, Brubacher JL, Newmark PA
  TITLE     A functional genomics screen in planarians reveals regulators of 
            whole-brain regeneration.
  JOURNAL   Elife. 2016 Sep 9;5. pii: e17002. doi: 10.7554/eLife.17002.
  PUBMED    27612384
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-09-9"; volume: "5. pii: e17002. doi"; pages: " 
            10.7554/eLife.17002"
COMMENT     Sequence Label: pRRG763-CRELD
FEATURES             Location/Qualifiers
     source          1..3765
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      8..596
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     497..516
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     750..767
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(779..826)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        840..858
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        862..880
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     862..879
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(1422..1440)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(1444..1462)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      1476..1523
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             1930..2724
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1984..2003)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2594..2613
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(2742..2810,2811..3602)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2960..2979)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
       61 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
      121 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
      181 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
      241 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
      301 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
      361 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
      421 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
      481 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
      541 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
      601 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
      661 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
      721 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcacgcgtca
      781 aaaaacccct caagacccgt ttagaggccc caaggggtta tgctaggcgc gccggatcct
      841 aatacgactc actataggga gatttaggtg acactataga agtgacctta gtacgcagca
      901 gaggcaagtg gtgaaaaatt tcagaacaaa ccaacaaaca aattgttgca tcgcttgttt
      961 aggctttgtt cggaaaatta accgtcctag tcattagatt ctgtttttgg gaaaaattaa
     1021 tgctttcctt ttaattcatt gaaattgcaa tacgaaaata taaaaaaaaa tttgatgtga
     1081 aataattgat tatttcagtt tttttttaat atattataat caaatatatt tctcgtctgt
     1141 catcgtcgat taaatttcgg attcactcga ccgatgtatt gctcaaaatg ttaccatgaa
     1201 cgaaaatata cccggctcga attagcaacc tggcaggata aaattccagt aatcgttggt
     1261 atagaagaaa ttgttaaaac aatatttgaa tattcatttg accaagtcga tgaagataag
     1321 cccattaaaa caacagtaat caaagactgg aatgaagaag aaatttactg tttgtgtgaa
     1381 aaaaagattt ccgaaatcaa caattctcac gagaaggtct ccctttagtg agggttaatt
     1441 ctccctatag tgagtcgtat taggatccgg cgcgcctagc ataacccctt ggggcctcta
     1501 aacgggtctt gaggggtttt ttgacgcgtc gatttcggcc tattggttaa aaaatgagct
     1561 gatttaacaa aaatttaacg cgaattttaa caaaattcag ggcgcaaggg ctgctaaagg
     1621 aagcggaaca cgtagaaagc cagtccgcag aaacggtgct gaccccggat gaatgtcagc
     1681 tactgggcta tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt
     1741 gggcttacat ggcgatagct agactgggcg gttttatgga cagcaagcga accggaattg
     1801 ccagctgggg cgccctctgg taaggttggg aagccctgca aagtaaactg gatggctttc
     1861 ttgccgccaa tgatctgatg gcgcagggga tcaagatctg atcaagagac aggatgagga
     1921 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     1981 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     2041 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     2101 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     2161 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     2221 ccggggcagg atctcctgtc atcccacctt gctcctgccg agaaagtatc catcatggct
     2281 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     2341 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     2401 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     2461 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     2521 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     2581 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     2641 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     2701 cgccttcttg acgagttctt ctgaattgaa aaaggaagag tatgagtatt caacatttcc
     2761 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     2821 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     2881 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     2941 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     3001 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     3061 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     3121 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     3181 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     3241 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     3301 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     3361 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     3421 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     3481 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agccaggcaa
     3541 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     3601 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     3661 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     3721 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaag
//
