LOCUS       sequence-192186-       11036 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Express yeast Kre33 with Y2H activation domain fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11036)
  AUTHORS   Vincent NG, Charette JM, Baserga SJ
  TITLE     The SSU processome interactome in Saccharomyces cerevisiae reveals 
            potential new protein subcomplexes.
  JOURNAL   RNA. 2017 Oct 20. pii: rna.062927.117. doi: 10.1261/rna.062927.117.
  PUBMED    29054886
REFERENCE   2  (bases 1 to 11036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2017 
            Oct 20. pii: rna.062927.117. doi: 10.1261/rna.062927.117."
COMMENT     Sequence Label: pACT2-Kre33
FEATURES             Location/Qualifiers
     source          1..11036
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..141
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(67..86)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     227..249
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        278..679
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             725..745
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             761..1102
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     CDS             1115..1141
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1121..1147
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     protein_bind    1181..1205
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4380..4404)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      4793..4980
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(4848..4872)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     protein_bind    complement(5001..5034)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(5543..6637)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(6638..7043)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     rep_origin      7520..8862
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(8483..8530)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        9127..9231
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9232..9300,9301..10092)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9450..9469)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10263..10851
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10752..10771
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11005..11022
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac
       61 tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta
      121 cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg
      181 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
      241 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agcaacttct tttctttttt
      301 tttcttttct ctctcccccg ttgttgtctc accatatccg caatgacaaa aaaatgatgg
      361 aagacactaa aggaaaaaat taacgacaaa gacagcacca acagatgtcg ttgttccaga
      421 gctgatgagg ggtatctcga agcacacgaa actttttcct tccttcattc acgcacacta
      481 ctctctaatg agcaacggta tacggccttc cttccagtta cttgaatttg aaataaaaaa
      541 aagtttgctg tcttgctatc aagtataaat agacctgcaa ttattaatct tttgtttcct
      601 cgtcattgtt ctcgttccct ttcttccttg tttctttttc tgcacaatat ttcaagctat
      661 accaagcata caatcaactc caagctttgc aaagatggat aaagcggaat taattcccga
      721 gcctccaaaa aagaagagaa aggtcgaatt gggtaccgcc gccaatttta atcaaagtgg
      781 gaatattgct gatagctcat tgtccttcac tttcactaac agtagcaacg gtccgaacct
      841 cataacaact caaacaaatt ctcaagcgct ttcacaacca attgcctcct ctaacgttca
      901 tgataacttc atgaataatg aaatcacggc tagtaaaatt gatgatggta ataattcaaa
      961 accactgtca cctggttgga cggaccaaac tgcgtataac gcgtttggaa tcactacagg
     1021 gatgtttaat accactacaa tggatgatgt atataactat ctattcgatg atgaagatac
     1081 cccaccaaac ccaaaaaaag agatctctat ggcttaccca tacgatgttc cagattacgc
     1141 tagcttgggt ggtcatatgg ccatggaggc cccagctttc acaagtttgt acaaaaaagc
     1201 aggctacaaa atggccaaaa aagctatcga ttcaagaatc ccgtcactga tcaggaatgg
     1261 tgttcaaacc aaacaaaggt ccatatttgt catcgttggg gacagggcac gtaaccagct
     1321 cccaaattta cattatttga tgatgagtgc tgaccttaag atgaacaagt ccgtcttatg
     1381 ggcttataag aagaagcttc taggattcac ttcgcacaga aaaaaaagag agaataaaat
     1441 taagaaagaa attaaaagag gtacaaggga agtgaatgaa atggaccctt ttgaatcttt
     1501 tatctctaac caaaatatca gatatgtata ctacaaagag agtgagaaaa tattgggtaa
     1561 tacctatggt atgtgtattc ttcaggactt cgaggcatta accccaaatc ttttagcaag
     1621 aaccattgaa actgtagaag gtggtggtat tgtggttatt ttattgaaat caatgtcatc
     1681 gttaaagcaa ctgtacacca tgactatgga tgttcacgcc cgttatcgta ctgaggctca
     1741 tggtgacgtt gttgcaagat ttaacgaaag attcatactt tctttaggtt caaatccaaa
     1801 ctgtttagtc gttgatgatg aattaaatgt tctaccgcta tctggtgcca aaaacgtgaa
     1861 accactacct cctaaggaag atgacgaatt gcctccaaag caattagagt tgcaagaatt
     1921 aaaagaatcc ttagaagatg tccaaccagc tggttcattg gtttctctat ccaaaactgt
     1981 caatcaagcc cacgctattc tttctttcat tgatgccatt tcggaaaaaa ctttgaattt
     2041 cacagttgca ctaacagctg gaagaggtag aggtaagtct gctgctttag gtatttctat
     2101 tgccgcggcc gtttctcacg ggtattctaa tatttttgtt acttctccat ctcccgaaaa
     2161 tttaaagact ctatttgagt ttattttcaa aggattcgat gccttaggtt accaagaaca
     2221 cattgattat gatatcattc aatctacgaa ccccgacttc aacaaagcca ttgtgagagt
     2281 tgatattaaa agagatcaca gacaaacgat tcaatatatt gttcctcaag accatcaagt
     2341 tctgggccaa gctgaattag ttgtcattga tgaagctgca gctattcctt tgccaatcgt
     2401 caagaacttg ctgggcccat atctagtctt catggcatcc actatcaatg gttacgaagg
     2461 tacgggtaga tctttatctc tgaagttaat tcagcaattg cgtaatcaaa acaacacatc
     2521 cggtcgggaa agtactcaaa ccgccgttgt ttcgagagat aataaggaaa aagactctca
     2581 cttacattct caatcccgtc aattacgtga aatttccttg gacgaaccaa tcagatatgc
     2641 tcctggtgac ccaattgaaa aatggttaaa caagctctta tgtttagatg ttactttaat
     2701 taaaaaccca agatttgcaa caagaggaac tcctcaccca tcccaatgta atctatttgt
     2761 tgtcaataga gatactctat tttcttatca tcctgtctca gaaaatttcc tggaaaaaat
     2821 gatggcactg tacgtttcat cccattacaa gaattctcca aacgacttac aactgatgag
     2881 tgacgcccca gcgcacaaac tatttgttct tttaccacca attgatccaa aggatggcgg
     2941 caggatccct gaccctctat gtgtcattca aattgcattg gaaggtgaaa tcagtaagga
     3001 gagtgtcaga aattcattat cgagaggcca aagagctggt ggtgatttaa tcccatggct
     3061 gatctctcaa caattccaag acgaggaatt cgctagtttg agtggtgctc gtatcgtcag
     3121 aattgccact aatcctgaat acgcatccat gggctatggt tctcgtgcaa ttgaattact
     3181 gagagactat ttcgagggta aattcactga catgtctgaa gatgttcgtc ctaaggatta
     3241 ttctataaag agggtgagcg ataaggaact ggccaaaact aatttattaa aggatgacgt
     3301 taaattaaga gacgcaaaaa cgttgccacc tctactatta aaactttctg aacagcctcc
     3361 tcattatcta cattatttgg gtgtttcata tggtttaacg caatcgttgc ataaattcag
     3421 gaagaataac agctttgttc ctgtttattt acgtcaaacc gcaaacgatt taactggtga
     3481 acatacttgt gttatgttga acgttttgga aggcagagaa tcaaactggc tcgttgaatt
     3541 tgccaaagat ttccgtaaaa gatttctgtc gcttctttct tatgattttc ataagtttac
     3601 tgccgttcaa gccttaagtg tcattgaaag tagtaagaaa gcacaagatt tgtctgatga
     3661 cgaaaagcat gataataaag aattgacgcg gacgcatctg gatgacattt tttctccatt
     3721 tgacttgaaa agattggata gctactcaaa taatttgcta gattatcacg ttatcgttga
     3781 tatgataccc atgcttgctc ttttgtactt cggcgataaa atgggagatt cagtaaaact
     3841 atcaagcgtc cagagtgcta tcctgttggc tatagggtta cagcgtaaaa acatcgacac
     3901 tattgccaag gaattgaatt taccttccaa tcaaactatt gctatgtttg cgaagatcat
     3961 gagaaaaatg tctcagtact tccgtcagct gttaagccaa tcaattgaag aaactctacc
     4021 aaatatcaaa gatgatgcta ttgctgaaat ggatggtgaa gaaatcaaaa attacaacgc
     4081 tgcagaagca ctggaccaaa tggaagaaga tttagaagaa gccggttctg aagccgttca
     4141 ggcgatgagg gaaaaacaaa aagagctaat caactccttg aacttagata aatacgccat
     4201 aaatgataac agtgaggaat gggctgaatc tcaaaaatct ttagaaatag ctgccaaggc
     4261 caaaggcgtc gtcagtttaa aaactggtaa aaagagaacg actgaaaagg ctgaagatat
     4321 ctatagacaa gagatgaaag ctatgaaaaa accaagaaag tctaaaaagg ctgcaaataa
     4381 cccagctttc ttgtacaaag tggtgagtcg agagatctat gaatcgtaga tactgaaaaa
     4441 ccccgcaagt tcacttcaac tgtgcatcgt gcaccatctc aatttctttc atttatacat
     4501 cgttttgcct tcttttatgt aactatactc ctctaagttt caatcttggc catgtaacct
     4561 ctgatctata gaatttttta aatgactaga attaatgccc atcttttttt tggacctaaa
     4621 ttcttcatga aaatatatta cgagggctta ttcagaagct ttggacttct tcgccagagg
     4681 tttggtcaag tctccaatca aggttgtcgg cttgtctacc ttgccagaaa tttacgaaaa
     4741 gatggaaaag ggtcaaatcg ttggtagata cgttgttgac acttctaaat aagcgaattt
     4801 cttatgattt atgattttta ttattaaata agttataaaa aaaataagtg tatacaaatt
     4861 ttaaagtgac tcttaggttt taaaacgaaa attcttattc ttgagtaact ctttcctgta
     4921 ggtcaggttg ctttctcagg tatagcatga ggtcgctctt attgaccaca cctctaccgg
     4981 catgccggtc gagggaccta ataacttcgt atagcataca ttatacgaag ttatattaag
     5041 ggttattgaa tatgatcgga attggtcgac tacgtcgtta aggccgtttc tgacagagta
     5101 aaattcttga gggaactttc accattatgg gaaatggttc aagaaggtat tgacttaaac
     5161 tccatcaaat ggtcaggtca ttgagtgttt tttatttgtt gtattttttt ttttttagag
     5221 aaaatcctcc aatatataaa ttaggaatca tagtttcatg attttctgtt acacctaact
     5281 ttttgtgtgg tgccctcctc cttgtcaata ttaatgttaa agtgcaattc tttttcctta
     5341 tcacgttgag ccattagtat caatttgctt acctgtattc ctttacatcc tcctttttct
     5401 ccttcttgat aaatgtatgt agattgcgta tatagtttcg tctaccctat gaacatattc
     5461 cattttgtaa tttcgtgtcg tttctattat gaatttcatt tataaagttt atgtacaaat
     5521 atcataaaaa aagagaatct ttttaagcaa ggattttctt aacttcttcg gcgacagcat
     5581 caccgacttc ggtggtactg ttggaaccac ctaaatcacc agttctgata cctgcatcca
     5641 aaaccttttt aactgcatct tcaatggcct taccttcttc aggcaagttc aatgacaatt
     5701 tcaacatcat tgcagcagac aagatagtgg cgatagggtt gaccttattc tttggcaaat
     5761 ctggagcaga accgtggcat ggttcgtaca aaccaaatgc ggtgttcttg tctggcaaag
     5821 aggccaagga cgcagatggc aacaaaccca aggaacctgg gataacggag gcttcatcgg
     5881 agatgatatc accaaacatg ttgctggtga ttataatacc atttaggtgg gttgggttct
     5941 taactaggat catggcggca gaatcaatca attgatgttg aaccttcaat gtaggaaatt
     6001 cgttcttgat ggtttcctcc acagtttttc tccataatct tgaagaggcc aaaacattag
     6061 ctttatccaa ggaccaaata ggcaatggtg gctcatgttg tagggccatg aaagcggcca
     6121 ttcttgtgat tctttgcact tctggaacgg tgtattgttc actatcccaa gcgacaccat
     6181 caccatcgtc ttcctttctc ttaccaaagt aaatacctcc cactaattct ctgacaacaa
     6241 cgaagtcagt acctttagca aattgtggct tgattggaga taagtctaaa agagagtcgg
     6301 atgcaaagtt acatggtctt aagttggcgt acaattgaag ttctttacgg atttttagta
     6361 aaccttgttc aggtctaaca ctacctgtac cccatttagg accacccaca gcacctaaca
     6421 aaacggcatc agccttcttg gaggcttcca gcgcctcatc tggaagtgga acacctgtag
     6481 catcgatagc agcaccacca attaaatgat tttcgaaatc gaacttgaca ttggaacgaa
     6541 catcagaaat agctttaaga accttaatgg cttcggctgt gatttcttga ccaacgtggt
     6601 cacctggcaa aacgacgatc ttcttagggg cagacattag aatggtatat ccttgaaata
     6661 tatatatata ttgctgaaat gtaaaaggta agaaaagtta gaaagtaaga cgattgctaa
     6721 ccacctattg gaaaaaacaa taggtcctta aataatattg tcaacttcaa gtattgtgat
     6781 gcaagcattt agtcatgaac gcttctctat tctatatgaa aagccggttc cggcgctctc
     6841 acctttcctt tttctcccaa tttttcagtt gaaaaaggta tatgcgtcag gcgacctctg
     6901 aaattaacaa aaaatttcca gtcatcgaat ttgattctgt gcgatagcgc ccctgtgtgt
     6961 tctcgttatg ttgaggaaaa aaataatggt tgctaagaga ttcgaactct tgcatcttac
     7021 gatacctgag tattcccaca gttccttacc actcttttgt tactctattg atccagctca
     7081 gcaaaggcag tgtgatctaa gattctatct tcgcgatgta gtaaaactag ctagaccgag
     7141 aaagagacta gaaatgcaaa aggcacttct acaatggctg ccatcattat tatccgatgt
     7201 gacgctgcag cttctcaatg atattcgaat acgctttgag gagatacagc ctaatatccg
     7261 acaaactgtt ttacagattt acgatcgtac ttgttaccca tcattgaatt ttgaacatcc
     7321 gaacctggga gttttccctg aaacagatag tatatttgaa cctgtataat aatatatagt
     7381 ctagcgcttt acggaagaca atgtatgtat ttcggttcct ggagaaacta ttgcatctat
     7441 tgcataggta atcttgcacg tcgcatcccc ggttcatttt ctgcgtttcc atcttgcact
     7501 tcaatagcat atctttgtta acgaagcatc tgtgcttcat tttgtagaac aaaaatgcaa
     7561 cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc atttttacag aacagaaatg
     7621 caacgcgaaa gcgctatttt accaacgaag aatctgtgct tcatttttgt aaaacaaaaa
     7681 tgcaacgcga gagcgctaat ttttcaaaca aagaatctga gctgcatttt tacagaacag
     7741 aaatgcaacg cgagagcgct attttaccaa caaagaatct atacttcttt tttgttctac
     7801 aaaaatgcat cccgagagcg ctatttttct aacaaagcat cttagattac tttttttctc
     7861 ctttgtgcgc tctataatgc agtctcttga taactttttg cactgtaggt ccgttaaggt
     7921 tagaagaagg ctactttggt gtctattttc tcttccataa aaaaagcctg actccacttc
     7981 ccgcgtttac tgattactag cgaagctgcg ggtgcatttt ttcaagataa aggcatcccc
     8041 gattatattc tataccgatg tggattgcgc atactttgtg aacagaaagt gatagcgttg
     8101 atgattcttc attggtcaga aaattatgaa cggtttcttc tattttgtct ctatatacta
     8161 cgtataggaa atgtttacat tttcgtattg ttttcgattc actctatgaa tagttcttac
     8221 tacaattttt ttgtctaaag agtaatacta gagataaaca taaaaaatgt agaggtcgag
     8281 tttagatgca agttcaagga gcgaaaggtg gatgggtagg ttatataggg atatagcaca
     8341 gagatatata gcaaagagat acttttgagc aatgtttgtg gaagcggtat tcgcaatatt
     8401 ttagtagctc gttacagtcc ggtgcgtttt tggttttttg aaagtgcgtc ttcagagcgc
     8461 ttttggtttt caaaagcgct ctgaagttcc tatactttct agagaatagg aacttcggaa
     8521 taggaacttc aaagcgtttc cgaaaacgag cgcttccgaa aatgcaacgc gagctgcgca
     8581 catacagctc actgttcacg tcgcacctat atctgcgtgt tgcctgtata tatatataca
     8641 tgagaagaac ggcatagtgc gtgtttatgc ttaaatgcgt acttatatgc gtctatttat
     8701 gtaggatgaa aggtagtcta gtacctcctg tgatattatc ccattccatg cggggtatcg
     8761 tatgcttcct tcagcactac cctttagctg ttctatatgc tgccactcct caattggatt
     8821 agtctcatcc ttcaatgcta tcatttcctt tgatattgga tcatatgcat agtaccgaga
     8881 aactagtgcg aagtagtgat caggtattgc tgttatctga tgagtatacg ttgtcctggc
     8941 cacggcagaa gcacgcttat cgctccaatt tcccacaaca ttagtcaact ccgttaggcc
     9001 cttcattgaa agaaatgagg tcatcaaatg tcttccaatg tgagattttg ggccattttt
     9061 tatagcaaag attgaataag gcgcattttt cttcaaagct caggtggcac ttttcgggga
     9121 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     9181 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     9241 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     9301 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     9361 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     9421 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
     9481 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     9541 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     9601 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     9661 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     9721 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca
     9781 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     9841 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     9901 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     9961 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
    10021 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
    10081 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
    10141 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
    10201 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
    10261 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
    10321 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
    10381 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
    10441 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
    10501 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
    10561 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
    10621 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
    10681 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
    10741 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
    10801 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
    10861 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
    10921 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
    10981 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcg
//
