LOCUS       sequence-19231-e         711 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf154
FEATURES             Location/Qualifiers
     source          1..711
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             377..418
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             428..445
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(468..485)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    474..701
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 agctcggatc cagtaccctt caccgaattc cagctggacc accatgacgc tcgtcatggg
       61 atcctgctgt ggtagattct gtgacgctaa gaataagaat aagaaggaag atgtagaaga
      121 gggaagagaa ggatgttaca attataagaa ccttaatgat ctggatgaat ccgaagcacg
      181 tgtagaattt ggaccattat atatgataaa tgaagaaaaa tcagacataa atacattgga
      241 tataaaaaga agatatagac acacgataga gtctgtatat ttctaagcaa ttcccgggga
      301 tccgaagggt caagacaatt ctgcagatat ccagcacagt ggcggccgct cgagtctaga
      361 gggcccgcgg ttcgaaggta agcctatccc taaccctctc ctcggtctcg attctacgcg
      421 taccggtcat catcaccatc accattgagt ttaaacccgc tgatcagcct cgactgtgcc
      481 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
      541 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
      601 gtgtcattct attctggggg gntgggggtg gggcaggaca gcaagggggg aggattggga
      661 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg c
//
