LOCUS       sequence-19240-e         705 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf163
FEATURES             Location/Qualifiers
     source          1..705
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             338..379
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             389..406
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(429..446)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    435..661
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gtacccttca ccgaattcca gctgaccacc atgtccgtcc tcataaacgg aacttgtttt
       61 ccagggttat tcattctctc gcatctggac ggggacgaag gtctcctctc acacgcgggc
      121 gatgagggtt tatctacttt gagtacgcag gaagctttac ttccttcgca ttctacagtt
      181 ctaacatcgc atactttaac ggcttttaga accagatatc ttccttcttt ggtattgata
      241 taagcaattc ccggggatcc gaataaaggg tcaagacaat tctgcagata tccagcacag
      301 tggcggccgc tcgagtctag agggcccgcg gttcgaaggt aagcctatcc ctaaccctct
      361 cctcggtctc gattctacgc gtaccggtca tcatcaccat caccattgag tttaaacccg
      421 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
      481 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
      541 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg gggtgggggt ggggcaggac
      601 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
      661 gcttctgagg cggaaagaac cagctggggc tctagggggt atccc
//
