LOCUS       sequence-192458-        2664 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  GoldenBraid vector pDGB2-alpha-1, adapted for S. cerevisiae genome 
            integration by flanking the GB cassette with recombination arms of 
            homology for integration at the YORW-Delta-22 solo LTR loci.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Perez-Gonzalez A, Kniewel R, Veldhuizen M, Verma HK, 
            Navarro-Rodriguez M, Rubio LM, Caro E
  TITLE     Adaptation of the GoldenBraid modular cloning system and creation of
            a toolkit for the expression of heterologous proteins in yeast 
            mitochondria.
  JOURNAL   BMC Biotechnol. 2017 Nov 13;17(1):80. doi: 
            10.1186/s12896-017-0393-y.
  PUBMED    29132331
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2017-11-13"; volume: "17(1):80. doi"; pages: " 
            10.1186/s12896-017-0393-y"
COMMENT     Sequence Label: YORW-Delta-22-alpha-1
FEATURES             Location/Qualifiers
     source          1..2664
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1162..1485)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     primer_bind     1167..1186
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1380..1402
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1394..1411
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1395..1411
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1412..1468
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(1481..1497)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1481..1497)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1494..1516)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1505..1521
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1529..1535,1536..1553,1554..1559))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1574..1595
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gcggccgcta gggataacag ggtaataccc actgccctga agtccagcag gtactgaggc
       61 ttggatctca aaagcgtggg gtgcgtgggc gtgcgctgct cgaggcattt tagccgctga
      121 tagtgctggc tccaggggcc actgcgcttg ccatacccgc acacggggtt ctttgaggca
      181 cttaattgac cggagattta aacgatattg tcgtcgggga ttagaagaca ttactggtgg
      241 ggaacccttg atgataatag gaaatatatg cgcagtatgc tccatcgtta cgagatgtta
      301 gcagcctcag agcatcctaa tgggaatatc aatgcataca caccggagct tggatatgat
      361 aaacgaaata ttcttgaatc gtgagatcgc ctgttttcaa aaccgttgga ggcagaaaca
      421 attttgtcac aagatgggca ttctacccca tccttgctgt attattgtag tctcgctttc
      481 ttttatgctg gacaaatgag actactgcac atttttatac gttcttggtt ttttttaaag
      541 gtgtggtttc ggcattatcc tgccgcacgt ttcttggata attcatcctg attctctatt
      601 ttaaacgctt cagcctatca ggatttggtt ttgatacata ctgcaagagt gtatctcggg
      661 aacagtcatt tattccgcaa caaacttaat tgcggaacgc gttaggcgat ttctagcata
      721 tatcaaatac cgttcgcgat ttcttctggg ttcgtctatt ttcttttaaa tacttattaa
      781 cgtactcaaa caactacact tcgttgtatc tcagaatgag atccctcagt atgacaatac
      841 atcattctaa acgttcgtaa aacacatatg aaacaacttt ataacaaagc gaacaaaatg
      901 ggcaacatga gatgaaactc cgcgtccctt agctgaacta cccaaacgta cgaatgcctg
      961 aacaattagt ttagatccga gattccgcgc ttccatcatt tagtataatc catattttat
     1021 ataatatata ggataagtaa cagcccgcga agaattcgtc tcaggagaga gacccacagc
     1081 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
     1141 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
     1201 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
     1261 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     1321 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     1381 ccagtcacga cgttgtaaaa cgacggccag tgaattcgag ctcggtaccc ggggatcctc
     1441 tagagtcgac ctgcaggcat gcaagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     1501 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     1561 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     1621 tccagtcggg aaacctgtcg tgccagctgg ggtctctcgc tgtcatgaga cgaattcgga
     1681 ccaactatca tccgctaatt actgacatta ccaaatgaga tctgtgaatg ggcaagataa
     1741 aaaacaaaaa ttgaaatgtt tgacgttatg taaaactatt aattccttcg ctttcggcgg
     1801 tcacagaatt tgcgtgtagc tgactcttgt tcaatcaata tcatttgtta ctttatttga
     1861 aagtctgtat tactgcgcct attgtcatcc gtaccaaaga acgtcaaaaa gaaacaagat
     1921 aatttttgtg cttacaccat ttatagatca ctgagcccag aatatcgctg gagctcagtg
     1981 taagtggcat gaacacaact ctgactgatc gcacatattg ccgttatcat aaatactagt
     2041 tgtacttgtc aatgcgacga atggcatcat gcctattatt acgttcctct ttttccgttt
     2101 catgtttcca gaatgctatt gaatctaaca cttcaattat aaaaaagaat aaatccgcaa
     2161 taattttagg ctaattgttg tactgtcaag cgaacctaat ggttaaaatt cagaggaacc
     2221 ttcgacgtag tctgatcgct acttctatat cttatgttcc cagtcaatca aaagttgata
     2281 ctataatagc tgccatttat acctgttagt tatggcgatc gtttatcacg acaagcccaa
     2341 ttttggtcga gatgcagcca gttgccatct attgtttcca aagcattcgt ttgattcgta
     2401 tatgatagga aaacaaatta aacgatgtat caatttgctc attcatcata aattgtgttt
     2461 gaggcattac taaaagagca gcagatatca gtctgttgga ataaaaatca actatcatct
     2521 actaactagt atttacgtta ctagtatatt atcatatacg gtgttagaag atgacgcaaa
     2581 tgatgagaaa tagtcatcta aattagtgga agctgaaacg caaggattga taatgtaata
     2641 gggataacag ggtaatgcgg ccgc
//
