LOCUS       sequence-192564-       11691 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Plasmid containing wild-type cas9sp.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11691)
  AUTHORS   van Raaphorst R, Kjos M, Veening JW
  TITLE     Chromosome segregation drives division site selection in 
            Streptococcus pneumoniae.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Jul 3. pii: 201620608. doi: 
            10.1073/pnas.1620608114.
  PUBMED    28674002
REFERENCE   2  (bases 1 to 11691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2017 Jul 3. pii: 201620608. doi: 
            10.1073/pnas.1620608114."
COMMENT     Sequence Label: pJWV102-wtcas9sp
FEATURES             Location/Qualifiers
     source          1..11691
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             390..4496
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDDYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRLNSEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLAKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFIKDDSIDNKVLTRSDKNRGKSNNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVRKDLIVKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATFIHQSITGLYETRIDLSQLGGD"
     primer_bind     complement(5638..5660)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    5746..5886
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     5801..5820
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5901..5918)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6072..6660)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6152..6171)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6831..7622,7623..7691))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7454..7473
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7692..7796)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7864..7882
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 atgcaatttc tatgcgcacc cgttctcgga gcactgtccg accgctttgg ccgccgccca
       61 gtcctgctcg cttcgctact tggagccact atcgactacg cgatcatggc gaccacaccc
      121 gtcctgtgga tctatcgatg catgcagccg gtaccatcac aagcactttg ggacgttctc
      181 ccttagtgct tttttgattt ctcataggcc ggcctgttag tcatatggac acttaaggca
      241 aattgttcag aactgaataa agctgacgtt ttgcttctat cctttctttg agttttagtg
      301 gataatgata atgaacaagg tgttcataaa tctattataa caagaattcc aatcccgggt
      361 aaagcttcca gcggccgcag gaggcaaaaa tggataagaa atactcaata ggcttagata
      421 tcggcacaaa tagcgtcgga tgggcggtga tcactgatga ttataaggtt ccgtctaaaa
      481 agttcaaggt tctgggaaat acagaccgcc acagtatcaa aaaaaatctt ataggggctc
      541 ttttatttga cagtggagag acagcggaag cgactcgtct caaacggaca gctcgtagaa
      601 ggtatacacg tcggaagaat cgtatttgtt atctacagga gattttttca aatgagatgg
      661 cgaaagtaga tgatagtttc tttcatcgac ttgaagagtc ttttttggtg gaagaagaca
      721 agaagcatga acgtcatcct atttttggaa atatagtaga tgaagttgct tatcatgaga
      781 aatatccaac tatctatcat ctgcgaaaaa aattggtaga ttctactgat aaagcggatt
      841 tgcgcttaat ctatttggcc ttagcgcata tgattaagtt tcgtggtcat tttttgattg
      901 agggagattt aaatcctgat aatagtgatg tggacaaact atttatccag ttggtacaaa
      961 cctacaatca attatttgaa gaaaacccta ttaacgcaag tggagtagat gctaaagcga
     1021 ttctttctgc acgattgagt aaatcaagac gattagaaaa tctcattgct cagctccccg
     1081 gtgagaagaa aaatggctta tttgggaatc tcattgcttt gtcattgggt ttgaccccta
     1141 attttaaatc aaattttgat ttggcagaag atgctaaatt acagctttca aaagatactt
     1201 acgatgatga tttagataat ttattggcgc aaattggaga tcaatatgct gatttgtttt
     1261 tggcagctaa gaatttatca gatgctattt tactttcaga tatcctaaga ttaaatagtg
     1321 aaataactaa ggctccccta tcagcttcaa tgattaaacg ctacgatgaa catcatcaag
     1381 acttgactct tttaaaagct ttagttcgac aacaacttcc agaaaagtat aaagaaatct
     1441 tttttgatca atcaaaaaac ggatatgcag gttatattga tgggggagct agccaagaag
     1501 aattttataa atttatcaaa ccaattttag aaaaaatgga tggtactgag gaattattgg
     1561 cgaaactaaa tcgtgaagat ttgctgcgca agcaacggac ctttgacaac ggctctattc
     1621 cccatcaaat tcacttgggt gagctgcatg ctattctgag aagacaagaa gacttttatc
     1681 catttttaaa agacaatcgt gagaagattg aaaaaatctt gacttttcga attccttatt
     1741 atgttggtcc attggcgcgt ggcaatagtc gttttgcatg gatgactcgg aagtctgaag
     1801 aaacaattac cccatggaat tttgaagaag ttgtcgataa aggtgcttca gctcaatcat
     1861 ttattgaacg catgacaaac tttgataaaa atcttccaaa tgaaaaagta ctaccaaaac
     1921 atagtttgct ttatgagtat tttacggttt ataacgaatt gacaaaggtc aaatatgtta
     1981 ctgagggaat gcgaaaacca gcatttcttt caggtgaaca gaagaaagcc attgttgatt
     2041 tactcttcaa aacaaatcga aaagtaaccg ttaagcaatt aaaagaagat tatttcaaaa
     2101 aaatagaatg ttttgatagt gttgaaattt caggagttga agatagattt aatgcttcat
     2161 taggtaccta ccatgatttg ctaaaaatta ttaaagataa agattttttg gataatgaag
     2221 aaaatgaaga tatcttagag gatattgttt taacattgac cttatttgaa gatagggaga
     2281 tgattgagga aagacttaaa acatatgctc acctctttga tgataaggtg atgaaacagc
     2341 ttaaacgtcg ccgttatact ggttggggac gtttgtctcg aaaattgatt aatggtatta
     2401 gggataagca atctggcaaa acaatattag attttttgaa atcagatggt tttgccaatc
     2461 gcaattttat gcagctgatc catgatgata gtttgacatt taaagaagac attcaaaaag
     2521 cacaagtgtc tggacaaggc gatagtttac atgaacatat tgcaaattta gctggtagcc
     2581 ctgctattaa aaaaggtatt ttacagactg taaaagttgt tgatgaattg gtcaaagtaa
     2641 tggggcggca taagccagaa aatatcgtta ttgaaatggc acgtgaaaat cagacaactc
     2701 aaaagggcca gaaaaattcg cgtgagcgta tgaaacgtat tgaagaaggt atcaaagaat
     2761 taggaagtca gattcttaaa gagcatcctg ttgaaaatac tcaattgcaa aatgaaaagc
     2821 tctatctcta ttatctccaa aatggaagag acatgtatgt ggaccaagaa ttagatatta
     2881 atcgtttaag tgattatgat gtcgatcaca ttgttccaca aagtttcatt aaagacgatt
     2941 caatagacaa taaggtctta acgcgttctg ataaaaatcg tggtaaatcg aataacgttc
     3001 caagtgaaga agtagtcaaa aagatgaaaa actattggag acaacttcta aacgccaagt
     3061 taatcactca acgtaagttt gataatttaa cgaaagctga acgtggaggt ttgagtgaac
     3121 ttgataaagc tggttttatc aaacgccaat tggttgaaac tcgccaaatc actaagcatg
     3181 tggcacaaat tttggatagt cgcatgaata ctaaatacga tgaaaatgat aaacttattc
     3241 gagaggttaa agtgattacc ttaaaatcta aattagtttc tgacttccga aaagatttcc
     3301 aattctataa agtacgtgag attaacaatt accatcatgc ccatgatgcg tatcttaatg
     3361 ccgtcgttgg aactgctttg attaagaaat atccaaaact tgaatcggag tttgtctatg
     3421 gtgattataa agtttatgat gttcgtaaaa tgattgctaa gtctgagcag gaaataggca
     3481 aagcaaccgc aaaatatttc ttttactcta atatcatgaa cttcttcaaa acagaaatta
     3541 cacttgcaaa tggagagatt cgcaaacgcc ctctaatcga aactaatggg gaaactggag
     3601 aaattgtctg ggataaaggg cgagattttg ccacagtgcg caaagtattg tccatgcccc
     3661 aagtcaatat tgtcaagaaa acagaagtac agacaggcgg attctccaag gagtcaattt
     3721 taccaaaaag aaattcggac aagcttattg ctcgtaaaaa agactgggat ccaaaaaaat
     3781 atggtggttt tgatagtcca acggtagctt attcagtcct agtggttgct aaggtggaaa
     3841 aagggaaatc gaagaagtta aaatccgtta aagagttact agggatcaca attatggaaa
     3901 gaagttcctt tgaaaaaaat ccgattgact ttttagaagc taaaggatat aaggaagtta
     3961 gaaaagactt aatcgttaaa ctacctaaat atagtctttt tgagttagaa aacggtcgta
     4021 aacggatgct ggctagtgcc ggagaattac aaaaaggaaa tgagctggct ctgccaagca
     4081 aatatgtgaa ttttttatat ttagctagtc attatgaaaa gttgaagggt agtccagaag
     4141 ataacgaaca aaaacaattg tttgtggagc agcataagca ttatttagat gagattattg
     4201 agcaaatcag tgaattttct aagcgtgtta ttttagcaga tgccaattta gataaagttc
     4261 ttagtgcata taacaaacat agagacaaac caatacgtga acaagcagaa aatattattc
     4321 atttatttac gttgacgaat cttggagctc ccgctgcttt taaatatttt gatacaacaa
     4381 ttgatcgtaa acgatatacg tctacaaaag aagttttaga tgccactttt atccatcaat
     4441 ccatcactgg tctttatgaa acacgcattg atttgagtca gctaggaggt gactaagtcg
     4501 acctcgagac tagtcaaggt cggcaattct gcagtactag gacgccgcca agccagctta
     4561 aacccagctc aatgagctgg gttttttgtt tgttaaaaat gaagaagaaa ctgtgaagcg
     4621 tatttatagc aaagcactca aaagtttacc ttatgggtgc ttttttcgtg cttttttgaa
     4681 aagacaaaaa aaagaacctt gccaagcaag attcttttga tagcgctatc gctgagcgcc
     4741 ggtcgctacc attaccagtt ggtctggtgt caaaaataat aataaccggg caggccatgt
     4801 ctgcccgtat ttcgcgtaag gaaatccatt atgtactatt tctggtgatg aaatcaacgt
     4861 aacatttaaa gctgtcaaag ccaaagtcat gagatggcgt atggagcgta aagctgacaa
     4921 gagcggtgtt gcgatgattg agatgacctt ccttgcacca agtgaattgc ctcaagaaag
     4981 cactcaatca aagattcttg tagatggaaa agaacttgct gatttcgctg aaaatcgtca
     5041 agactatcaa attacctata aaggtcaacg gccaaaagtc tcagttgaag aaaacaatca
     5101 agtagcttca actgtggtag atagtggaga agatagcctt ccagtacttg ttcgcctcgt
     5161 ttcagaaagt ggaaaacaag tcaaggaata ccgtatccag ttgactaagg aaaaaccagt
     5221 ttctgctgta caagaagatc ttccaaaact cgaatttgtt gaaaaagatt tggcctacaa
     5281 gacagttgag aaaaaagatt caacactgta tctaggtgaa actcgtgtag aacaagaagg
     5341 aaaagttgga aaagaacgta tctttacagt gattaatcct gatggaagta aggaagaaaa
     5401 actccgtgaa gtggtagaag ttccgacaga ccgcatcgtc ttggttggaa ccaaaccagt
     5461 agctcaagaa gctaaaaaac cacaagtgtc agaaaaagca gatacaaaac caattgattc
     5521 aagtgaagct gatcaaacta ataaagccca gttaccaaat acaggtagtg cggcaagcca
     5581 agcagcagta gcagcaggtt tagcctgcct cgcgcgtttc ggtgatgacg gtgaaaacct
     5641 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     5701 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca
     5761 gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta
     5821 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     5881 atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     5941 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     6001 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     6061 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     6121 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     6181 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     6241 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     6301 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     6361 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     6421 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     6481 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     6541 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     6601 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     6661 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     6721 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     6781 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     6841 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     6901 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     6961 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     7021 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     7081 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     7141 attgctgcag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     7201 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     7261 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     7321 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     7381 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     7441 gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     7501 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     7561 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     7621 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     7681 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     7741 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     7801 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     7861 aaaaataggc gtatcacgag gccctttcgt cttcaagaat taattccttc ttaacgcccc
     7921 aagttcatca ccaatgacat caactcacat gaactacatg atgaacccag ttatcatggt
     7981 tttggataag atttttgaaa aattcttccc aggccttgat aaatatgact ttgatgctgc
     8041 taaattgaac aagaaaatcg gtttctgggg atctaaattc ttcatcggtt tcatccttgg
     8101 tatcgttatc ggtattatgg gaactccaca tccaattgca ggtgttgcag atgcagataa
     8161 atggcgtctt gttatcaaag gatggttgtc tcttggtttg actgccggtg tatctttgga
     8221 actcttctca cttatcggtt catggttcat cgcagccgta gaaccactat cacaaggtat
     8281 tacaaacgtt gctactaaac gtcttcaagg acgtaaattc aatatcggtc ttgactggcc
     8341 attcatcgct ggtcgtgctg aaatctgggc ttgtgccaac gtacttgcac caatcatgtt
     8401 gattgaagca gtgcttcttt caaaagttgg aaatggtatc ttgccacttg caggtatcat
     8461 cgctatgggt gttactccag ctctcttggt tgtaactcgt ggtaaattgc tccgtatgat
     8521 tatcttcgga acactcttgt tgccactctt ccttctttca ggtacactta ttgcaccatt
     8581 tgcaacagaa cttgctaaag gtgtaggtgc cttcccagaa ggtgtgagcc aaactcaatt
     8641 gattactcac tctactcttg aaggaccaat cgaaaaactt cttggttgga caattggtaa
     8701 cactacaact ggtgatatca aagcaatcct tggtgcagta gccttccttg tattctatat
     8761 cggtatcttt gcttggtaca gaaaacaaat gatcaaacgt aacgaagagt acgcagcaaa
     8821 agcaaaataa tgcgctccgc tagctttaca gacaaagaac tatccttaat ggaagcacta
     8881 gcaaaaggta tcaatgaagc aagaaatatt gaaggctagt cagtaaaatt cagataaaca
     8941 aaaagagccg ataagatgag agtttcattt ctcaaattat cggctctgcg tcttggcgtc
     9001 tggctctttg attgttatta tattcatttt ttgaacatta attaaagttt cgttcttaca
     9061 tttggggcag tataaaggga aatttttaag tatagtatcc actcgtattc gtagtcttgt
     9121 tttatttcca caaataggac acaatatcca cttgtagttt ataataacaa tctcctcctt
     9181 tccactttaa ttcaaatcta tattaaagaa tatttcatct tatttaataa gaaaccatat
     9241 ttatataaca acataaaacg cactaagtta ttttattgaa catatatctc actttatcta
     9301 tctgactatt tagacgacgg gtctggcaaa caggttcgcc agtggtaacc tgatatcctt
     9361 ttagctctgc taaacaaaca ctaagcccat ttgtaaaaaa agttaaatca ttgcgataat
     9421 cttgaataca tcgagcagga atttctccaa taataatgac ctcattattt ttcagttgag
     9481 tatttacgat atttgcacaa tatttgggag catcgttata tgcccgtgaa agatattcct
     9541 gtggtgcata aactttaaaa ctaagatatg gctctaacaa ttctgttcca gcttttctaa
     9601 aggcttgctc caatacaata ggagcaagca tccgaaaatc tgctggggta ctaacagggc
     9661 tatagtataa gccatactta aaacagattt tacagtccgt cacattccaa ccatacaatc
     9721 cttgttcaca accatagcgt atcccttcca taactgcatt ttgaaatgat tgatttaagt
     9781 atccaagaga aaccgagctc tcatactgca ttccacttcc caacggaagc ggtgatacag
     9841 ataaaccaat ggaagcccag aaaggatttg gcggcacttc gatgtgaatg gtatattctg
     9901 cattttttaa cggtctctcc atataaatga ctgtaggctc ttttagttct atctccacat
     9961 gatacttttc ttgcaacagt gcactaatca cttccatttg tactttccct aagaaagaaa
    10021 gtataatttc atgtgtcgta gaatccacgt aatatcgtag aagcggatca ctatctgaga
    10081 tttccaaaag ggcatcaagc aacatttctc tctgttcagg tttactcggt tcaacagttg
    10141 tttgtagtag agggtgcgga ttttcaatct tttttctctg tggcaatagt tttgtatctc
    10201 caagaacact atttaacttc aaaagctcat tttgcaaaat aacaatttct ccagaataag
    10261 ctcgatcaat cttacataat tcaccattta ttgaagtata catttctgta acttttattt
    10321 tttctttttc tgatactcta accgaatctc gtaaatgtag tactccacta taaaggcgta
    10381 tatatgcaag acgttgtctt ttttttgtat attcaatttt gaaaacattt ccgcaaagtt
    10441 cagacggacc tcgatgtgtt gatgaataaa atttattagt aataacttct ataaggttat
    10501 caatccctat attacttttt gcacttccat gataaagagg gaacagagaa caattctgaa
    10561 atcttatgct ttcctcttgt tcgagttcca atgcttctaa tgatttaccg gacatatatt
    10621 tctctaaaag gtcatcgttt ccctctatta ccgtatccca ttgttcagat tcggtaaagt
    10681 tcgtcacaca cacattagga tacagttcta ccttctgttt gattacaatt tcggcagaaa
    10741 gtttctcttt aatatcctga taaaccgttg ataaatcaat tccattttgg tcaatcttat
    10801 tgataaaaaa gattgtggga atccccattt tcctaagtgc atgaaataat atacgagttt
    10861 gtgcttgtac gccatctttt gcagaaatca gtagaattgc cccatctaaa actgataatg
    10921 aacgatatac ttctgctaag aaatccatat gtcctggcgt gtctatgatg ttcaccttcg
    10981 tattttccca ctgaaaagag gttattcctg tctgaattgt aattcctctc tgacgttcta
    11041 aaagcgtatt atccgtcctc gttgtacctt tgtccacgct tcctaattct gtaatcgctc
    11101 cactgttata taataagctt tctgttaagg tagtttttcc tgcatcaaca tgagctaaaa
    11161 ctccaatatt aataattttc atgtgatttt cctccattca aaagcccaaa agggcataaa
    11221 aatcccagtg ataaatactc ttatcactgg gatttttatg cataaccata ggcatacaaa
    11281 gcatacagat attctccgga tactttagaa tcacatgata aaggtattct taaactgggg
    11341 acaaaaaact aagccctcct aaaaaaggac gtccaattat ttgttcccgc tatcaaattg
    11401 acagtttatt taagaatacc ttgccgcata tttattaact ccttttaaat agatacttaa
    11461 attatagcac gtaagagcat atttgtaaag gaatctccaa ttttttatca aaaagagtac
    11521 atgattacaa agtatctgta atcatgtacc aatatttatt attttacaat cttccaatta
    11581 tcttgttttt ctaatatcaa ctcaaattgc gagatttggg ttgcctttgt ttcttgatct
    11641 aaatatttca cagcgacatt cacgattact tggctagcgc tatatgcgtt g
//
