LOCUS       sequence-192930-        3778 bp    DNA     circular SYN 15-APR-2025
DEFINITION  part designed to occupy position 1 of EMMA. Functional category: 
            Long Terminal Repeat.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3778)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 3778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016."
COMMENT     Sequence Label: YCe3777 HC_Kan_Chimeric-5'LTR_p1
FEATURES             Location/Qualifiers
     source          1..3778
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        82..308
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             309..489
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    536..661
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1154..1387
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1572..1616
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1914..2031
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     terminator      2126..2157
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        2158..2260
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             2261..3076
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2334..2353)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      3097..3124
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(3136..3723)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3216..3235)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3745..3774)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 agttgattgc agtccagtta cgctggagtc tgaggctcgt cctgaatgat atcaagcttg
       61 aattcgttac gtctcgtagg atgtagtctt atgcaatact cttgtagtct tgcaacatgg
      121 taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg
      181 tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga
      241 ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat
      301 acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
      361 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
      421 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
      481 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
      541 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
      601 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
      661 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
      721 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
      781 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
      841 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
      901 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
      961 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
     1021 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     1081 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     1141 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1201 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1261 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1321 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     1381 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     1441 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     1501 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     1561 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     1621 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     1681 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     1741 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     1801 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     1861 gatcggcact gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa
     1921 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     1981 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     2041 tacagggaat ggcgagacga agacgaattc tctagatatc gctcaatact gaccatttaa
     2101 atcatacctg acctccatag cagaaagtca aaagcctccg accggaggct tttgacttga
     2161 tcggcacgta agaggttcca actttcacca taatgaaata agatcactac cgggcgtatt
     2221 ttttgagtta tcgagatttt caggagctaa ggaagctaaa atgagccata ttcaacggga
     2281 aacgtcttgc tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa
     2341 atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc
     2401 cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga
     2461 tgagatggtc aggctaaact ggctgacgga atttatgcct cttccgacca tcaagcattt
     2521 tatccgtact cctgatgatg catggttact caccactgcg atcccaggga aaacagcatt
     2581 ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt
     2641 cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacggcg atcgcgtatt
     2701 tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttggtgcga gtgattttga
     2761 tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata agcttttgcc
     2821 attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga
     2881 cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca
     2941 ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct
     3001 ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc acttgatgct
     3061 cgatgagttt ttctgagggc ccaaatgtaa tcacctggct caccttcggg tgggcctttc
     3121 tgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgatg
     3181 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     3241 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     3301 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     3361 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     3421 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     3481 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     3541 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     3601 gctgaagcca gttacctcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     3661 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     3721 caagaagatc ctttgatttt ctaccgaaga aaggcccacc cgtgaaggtg agccagtg
//
