LOCUS       sequence-192977-       10468 bp    DNA     circular SYN 14-APR-2025
DEFINITION  mKate2^SEC^3xMyc vector with ccdB sites for cloning homology arms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10468)
  AUTHORS   Dickinson DJ, Pani AM, Heppert JK, Higgins CD, Goldstein B
  TITLE     Streamlined Genome Engineering with a Self-Excising Drug Selection 
            Cassette.
  JOURNAL   Genetics. 2015 Jun 3. pii: genetics.115.178335.
  PUBMED    26044593
REFERENCE   2  (bases 1 to 10468)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2015 Jun 3. pii: genetics.115.178335."
COMMENT     Sequence Label: pDD287 (mKate2^SEC^3xMyc
FEATURES             Location/Qualifiers
     source          1..10468
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     117..136
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     370..387
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    504..525
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(540..545,546..563,564..570)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    578..594
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     583..605
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     602..618
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     602..618
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             891..1196
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(1241..1330)
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     protein_bind    1349..1382
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    1735..1759
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(1760..2785)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(1760..2785)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(2788..2812)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      4730..5053
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     CDS             complement(6871..6897)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    6907..6940
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             complement(6958..6978)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(7899..8204)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(8470..8487)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8470..8486)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8479..8501)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8695..8714)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8814..8836
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8874..8892)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8960..9064
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9065..9133,9134..9925)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9283..9302)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(10096..10468,1..216)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
       61 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
      121 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
      181 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
      241 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
      301 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
      361 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
      421 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
      481 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
      541 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
      601 acaggaaaca gctatgacca tgttatcgat ttcctaggaa cccgaagtat gtcaaaaaga
      661 ggtatgctat gaagcagcgt attacagtga cagttgacag cgacagctat cagttgctca
      721 aggcatatat gatgtcaata tctccggttc ggtaagcaca accatgcaga atgaagcccg
      781 tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg
      841 gtttattgaa atgaacggct cttttgccga cgagaacagg ggctggtgaa atgcagttta
      901 aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata
      961 ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag
     1021 ataaagtccc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga
     1081 tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca
     1141 gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt
     1201 caggctccct tatacacagc cagtctgcag gtcgagccgg caagtcttcc tcgctgatca
     1261 acttctgctc gaggtcctcc tcggagatga gcttttgctc aagatcctct tcagaaataa
     1321 gtttttgttc agatccggct ccctgaaaat aacttcgtat aaagtatcct atacgaagtt
     1381 attaaaaatt gcggtcataa actgaaacgt aacatatgat aaggtatttt gtgtgcggtt
     1441 ttttctatga tgtttcaaaa aaatttatca gaagtaaaaa acataagaag tgtacacaga
     1501 acattgtgta gattttggga tagaattatt attaatgggg ggagggagca caattttttt
     1561 cgacttttta ttacgaatta attctatttt tgaatctaca caatttcatt gttagaggtg
     1621 acttaaaaga agctaaaaaa caaagaaatt aagagaagtt ttttgataat aacaaaaata
     1681 gggggtggga gcacagggag aaagagcatg tagggatgtt gaagagtaat tggacccact
     1741 ttgtacaaga aagctgggtc tattcctttg ccctcggacg agtgctgggg cgtcggtttc
     1801 cactatcggc gagtacttct acacagccat cggtccagac ggccgcgctt ctgcgggcga
     1861 tttgtgtacg cccgacagtc ccggctccgg atcggacgat tgcgtcgcat cgaccctgcg
     1921 cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg atagagttgg tcaagaccaa
     1981 tgcggagcat atacgcccgg agccgcggcg atcctgcaag ctccggatgc ctccgctcga
     2041 agtagcgcgt ctgctgctcc atacaagcca accacggcct ccagaagaag atgttggcga
     2101 cctcgtattg ggaatccccg aacatcgcct cgctccagtc aatgaccgct gttatgcggc
     2161 cattgtccgt caggacattg ttggagccga aatccgcgtg cacgaggtgc cggacttcgg
     2221 ggcagtcctc ggcccaaagc atcagctcat cgagagcctg cgcgacggac gcactgacgg
     2281 tgtcgtccat cacagtttgc cagtgataca catggggatc agcaatcgcg catatgaaat
     2341 cacgccatgt agtgtattga ccgattcctt gcggtccgaa tgggccgaac ccgctcgtct
     2401 ggctaagatc ggccgcagcg atcgcatcca tggcctccgc gaccggctgc agaacagcgg
     2461 gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg tgcacggcgg gagatgcaat
     2521 aggtcaggct ctcgctgaat tccccaatgt caagcacttc cggaatcggg agcgcggccg
     2581 atgcaaagtg ccgataaaca taacgatctt tgtagaaacc atcggcgcag ctatttaccc
     2641 gcaggacata tccacgccct cctacatcga agctgaaagc acgagattct tcgccctccg
     2701 agagctgcat caggtcggag acgctgtcga acttttcgat cagaaacttc tcgacagacg
     2761 tcgcggtgag ttcaggcttt ttcatggagc ctgctttttt gtacaaactt gcggcaccgc
     2821 ctgacatatt accttaaaat tcaaaaatta atttcagatc agtcataacc aagaaaacaa
     2881 aataaacgtt aggacagaaa attcaaatta attaaaaatt atgctgaata agaaaattac
     2941 atgcaaagcc ttattttcct catagaaaca gttgaataac atatttttta attgaaaaca
     3001 tagcaaaatt cgtggtaaaa tttgtcaaaa ccttggaaaa aatcgaaaaa agaacatttc
     3061 ccgatgcatt ttcctatttc tcgttgccga caacgtataa acgttgaata ctcagtcaac
     3121 gaatttggct gctggcttgg actctcgcac ggaagagacg cgtgtgtaga tttacgacga
     3181 aagcaaaaat gatcaccaaa aacggaacgt tgagctggac ggaaatagtg gtaaagtgac
     3241 atgattatag tttgaagatt tctaatttca caattagagc aaatgttgtt cggtatttat
     3301 tttcaacggt atttatacta ttttccacct ttttctagaa cattcgagct gcttgttgca
     3361 aaaggagggc gactcacatt cggtacatgg aaaagtagtg tacacaataa agagacccag
     3421 atacattttc cgtctgcgtc tctttgcacc caccgggagt attttcaaac gaatgcatct
     3481 aggaccttct agaacattct gtaaggctgc agaatgcggg tatataagga aagcgggctc
     3541 agaggaagcc aacacgcttt gttctagtgc atctaaaaaa cttcgaaaat atgtcaaatc
     3601 ttctcactgt tcatcaaaat cttccagcac ttccagttga cgcgacaagt gatgaagttc
     3661 gcaagaacct catggatatg ttccgtgaca gacaagcctt ctccgagcac acctggaaga
     3721 tgcttctctc agtgtgccgt tcttgggctg cctggtgcaa gcttaacaac cgcaagtggt
     3781 tcccagccga gccagaagac gttcgtgact acttgctcta cctccaagct cgtggattgg
     3841 cggtgaagac gatccaacaa catctcggac agctcaatat gctccatcgc cgatctggac
     3901 ttccacgccc atcagattct aatgccgtat ctctcgtaat gagacgtatc cgcaaggaaa
     3961 atgttgatgc tggagagaga gccaagcaag ccctcgcctt cgaaaggtaa gtttaaatat
     4021 taatgtattc aatcctaaga cttgacgaaa ttttcagaac cgacttcgat caggtccgtt
     4081 ctcttatgga aaattccgat cgttgccagg acatccgaaa cctcgccttc ctcggaattg
     4141 cttacaacac acttctccgt atcgccgaaa ttgcccgcat tcgcgtcaag gatattagtc
     4201 gcacagacgg aggaagaatg ctcatccaca tcggacgtac aaaaacgctg gtctcgactg
     4261 ccggagtcga aaaagcgctc tcccttggag ttaccaagct cgttgagcgc tggatttcgg
     4321 tttccggagt cgccgatgat ccaaacaact acctgttctg tcgtgttcgc aagaacggag
     4381 ttgctgcccc gagtgcaaca tcacaactct cgactcgagc tcttgaaggt aagtttaaac
     4441 aacatattag ctaagaataa gctgaaaata aattttcagg aatcttcgaa gctacccacc
     4501 gtctcatcta cggagccaag gatgattccg gacaacgtta cttggcttgg tctggacatt
     4561 ccgcccgtgt cggagctgcc cgtgacatgg ctcgcgctgg agtttccatc ccagagatta
     4621 tgcaagccgg aggttggacc aacgtcaaca ttgtgatgaa ctatattaga aacttggatt
     4681 ctgaaactgg cgccatggtt cgtcttctcg aagacggtga ttaagataaa tgcaaaatcc
     4741 tttcaagcat tcccttcttc tctatcactc ttctttcttt ttgtcaaaaa attctctcgc
     4801 taatttattt gcttttttaa tgttattatt ttatgacttt ttatagtcac tgaaaagttt
     4861 gcatctgagt gaagtgaatg ctatcaaaat gtgattctgt ctgatgtact ttcacaatct
     4921 ctcttcaatt ccattttgaa gtgctttaaa cccgaaaggt tgagaaaaat gcgagcgctc
     4981 aaatatttgt attgtgttcg ttgagtgacc caacaaaaag aggaaacttt attgtgccgc
     5041 caagaaaaaa gtctcactga atctcaaata ttttattaga aaacaccaac aatttagata
     5101 tttctgtatc cacggttggc gttgacagcc tctgatggtg gagacttgtc tgggcactgg
     5161 cagtactcgg catcctttcc tggtcttccg tctggtccag tctctccctg ggttccgtct
     5221 ggtccttgac gtccttgctt tccagatggt ccttctggtc caacggttcc tggctctcct
     5281 ggtggtccgt gaagaccgtt ctgtccagct ggtcctgttg gtccttgatc tcctcttggt
     5341 ccctttggtc ctggtcttcc gattggatgc tcggcgtcgc gtcccttctc tcctggtggt
     5401 cctggctctc cgatctttcc gatttgaccg gatgatcctt gctctcctgg atgtcctggt
     5461 tgtccatctc ttcctgggtt tccattttgt cctcttggtc ctggaagtcc acgtggtcct
     5521 ggtcttccaa gagctcctgg tggtccaact ggtccttgtg ggcaagtgaa gcatccgaca
     5581 gactcgcgtt gtggagcgat atcatcagct ccaactcctg gaacaccgtc aagtcctgga
     5641 actccgtcga gtcctggaac tcctggaact ccctttggtc cggatggtcc agctgggcac
     5701 ttgttatcag cattgcagtt gcaggttggt ccatttccat tggctccatt tggagtcttt
     5761 ggctggttga agactggtgg aactgctggt ggttgacctg gtggagcaga tggggatcca
     5821 tcagcatgtg gagttggggt agcgttggtc tcttcatatt gacaacggac acgcttgcta
     5881 gatcttccga tgacaactat gtccttccac aatccattgg tagattgcta gaaaattaaa
     5941 aattatttaa aattcaaaat ataatttaac tcactctgaa cacattcatc tcggtatcaa
     6001 gcttctctct gaatccatca acctccgagt agatggttga catggtcatg aagcatacca
     6061 taagagtggc gacagtgacc gaagccgtca cataacacgc aagttttaca gacatctctg
     6121 aaattatcaa atttaggatt tcaaaataat tcggccaaag aaatgtaact cacctagtct
     6181 tcgagaagca gagacttgtc gatttcctac cggattcgtc tccttttatt aggaaatctt
     6241 tccgcaagtt ttcaagtttt ttttgttttt tttttacatt accgacgaga cagtgtgcga
     6301 gacgcagaga cgataaaaga ctggataaga acgggagata aggagtacgg atgtaggtga
     6361 ggaatgtggc cgaaaggaca gatgggcttt attcgggaag attggttggg aaatgaacca
     6421 gagaagaatg actgaataaa gttgttgaaa cttggtgaga atggaagata gacaatcgag
     6481 ggcagctagt tttccttcct cctctatata tttttatgtt ttcttttaaa ccttcctcca
     6541 tatttctgaa ttgaatgttg ttgcatattg tgtgtcctta tttcatttcc atcatccttt
     6601 tgtgtgtttc ccccttccac caattccccc cacgtcctaa tgaaatgaaa gcgagaagat
     6661 aaataaactt gcacattgtg ttcagatgag aggagcggaa tacgaattgg gagacggaaa
     6721 gagaaaaaaa aatgtttaga tttggattga attaattttt tagaaagcaa gaaaaaaaac
     6781 acaaagagaa taaataaata ataggaaaaa aaacaaatat tcagtattta aaatttgaaa
     6841 attcaacgac gttggcgtcg atcatcctta ggcgtagtct gggacgtcgt atgggtatcc
     6901 ctgaaaataa cttcgtataa agtatcctat acgaagttat tttaaactta cctttccgga
     6961 ttgaaagtac agattctcac ggtgtccgag cttggatggg aggtcgcagt aacgggcgac
     7021 ggcgacctcg tgttgctcga cgtaggtctc cttgtcggcc tccttgatac gctcaagacg
     7081 acggtcgacg tagtagactc ctggcatctt gaggttcttg gctggcttct tggaacggta
     7141 ggtggtctga aaatttaaat cagattagtt agttagtaaa atcatgttta aacttacctt
     7201 gaggttgcag atgaggtgtc ctcctccgac gagcttgagg gccatgtcgg cacgtccctc
     7261 aagtcctccg tcggctgggt agagggtctc ggtggaggcc tcccatccga gggtcttctt
     7321 ttgcatgact ggtccgttgg atgggaagtt gactccacgg atctgaaaat ttaaatatgt
     7381 atggttagtt agtaccgaac tgtttaaact taccttgacg ttgtagatga ggcatccgtc
     7441 ttggagggag gtgtcttggg tggcggtgag gactcctccg tcctcgtagg tggtgacacg
     7501 ctcccaggtg aatccctctg ggaaggattg cttgaagaag tctgggattc cttgggtgtg
     7561 gttgatgaag gtctgaaaat ttaaataatc agggttagtt agtatatata tgtttaaact
     7621 taccttggat ccgtacatga aggaggtggc gaggatgtcg aaggcgaatg ggagtggtcc
     7681 tccctcgacg gccttgatac gcatggtttg ggttccctcg tatggctttc cctctccctc
     7741 ggaggtgcac ttgaagtggt ggttgttgac ggttccctcc atgtagagct tcatatgcat
     7801 gttttcttta atgagctcgg agaccatcga tgctcctgag gctcccgatg ctccctaggt
     7861 cgacctgcag actggctgtg tataagggag cctgacattt atattcccca gaacatcagg
     7921 ttaatggcgt ttttgatgtc attttcgcgg tggctgagat cagccacttc ttccccgata
     7981 acggagaccg gcacactggc catatcggtg gtcatcatgc gccagctttc atccccgata
     8041 tgcaccaccg ggtaaagttc acgggggact ttatctgaca gcagacgtgc actggccagg
     8101 gggatcacca tccgtcgccc gggcgtgtca ataatatcac tctgtacatc cacaaacaga
     8161 cgataacggc tctctctttt ataggtgtaa accttaaact gcatttcacc agcccctgtt
     8221 ctcgtcggca aaagagccgt tcatttcaat aaaccgggcg acctcagcca tcccttcctg
     8281 attttccgct ttccagcgtt cggcacgcag acgacgggct tcattctgca tggttgtgct
     8341 taccgaaccg gagatattga catcatatat gccttgagca actgatagct gtcgctgtca
     8401 actgtcactg taatacgctg cttcatagca tacctctttt tgacatactt cgggtaacta
     8461 gtgccggcga ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     8521 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     8581 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta
     8641 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat
     8701 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     8761 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     8821 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
     8881 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     8941 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     9001 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     9061 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     9121 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     9181 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     9241 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     9301 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     9361 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     9421 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     9481 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     9541 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     9601 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     9661 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     9721 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     9781 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     9841 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     9901 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     9961 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
    10021 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
    10081 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
    10141 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
    10201 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
    10261 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
    10321 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
    10381 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
    10441 cttggagcga acgacctaca ccgaactg
//
