LOCUS       sequence-193278-        8107 bp    DNA     circular SYN 15-APR-2025
DEFINITION  N-term 1-1011 of HCF-1 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8107)
  AUTHORS   Wilson AC, Freiman RN, Goto H, Nishimoto T, Herr W
  TITLE     VP16 targets an amino-terminal domain of HCF involved in cell cycle 
            progression.
  JOURNAL   Mol Cell Biol. 1997 Oct;17(10):6139-46.
  PUBMED    9315674
REFERENCE   2  (bases 1 to 8107)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "1997-10"; volume: "17(10)"; pages: "6139-46"
COMMENT     Sequence Label: pCGN-HCF-1-N1011
FEATURES             Location/Qualifiers
     source          1..8107
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        304..507
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     458..478
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     504..528
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     5'UTR           597..636
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             666..692
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             672..698
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             3128..3145
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     intron          3805..4377
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(4509..4528)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4574..4629
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4628..4647)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      4983..5118
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5224..5241)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5395..5983)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5475..5494)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6154..6945,6946..7014))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6777..6796
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7015..7119)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7187..7205
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7243..7265)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7365..7384
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(7451..7906)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7588..7609)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7800..7819
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8032..8054
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8046..8063
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8047..8063
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8070..8089
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8070..8088
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        join(8107,1..303)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
       61 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      121 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      181 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      241 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      301 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      361 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      421 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
      481 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
      541 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccgggggatc
      601 ttggtggcgt gaaactcccg cacctcttcg gccagcgcct tgtagaagcg cgtatggctt
      661 ctagctatcc ttatgacgtg cctgactatg ccagcctggg aggaccttct agatcacgtc
      721 gtgcatctgg tacgtacgga cgcgtcgctt cggccgtgtc gcccgccaac ttgccagcgg
      781 tgcttctgca gccccgctgg aagcgagtgg tgggctggtc gggtccggtg ccacggcccc
      841 gccacggcca ccgcgccgtg gccatcaagg agctcatcgt ggtgtttggc ggcggcaacg
      901 agggaatagt ggacgaactg cacgtgtaca acacggcaac caaccagtgg ttcatcccag
      961 ccgtgagggg ggacattccc cctgggtgtg cagcctatgg cttcgtgtgt gacgggactc
     1021 gcctcctggt gtttggtggg atggtggagt atgggaaata cagcaatgac ctctacgaac
     1081 tccaggcgag ccggtgggag tggaagagac tcaaagcaaa gacgcccaaa aacgggcccc
     1141 ctccgtgtcc tcgactcggg cacagcttct cccttgtggg caacaaatgc tacctgtttg
     1201 ggggtctggc caatgatagc gaggacccaa agaacaacat tccaaggtac ctgaatgact
     1261 tatatatcct ggaattacgg ccaggctctg gagtggtagc ctgggacatt cccatcactt
     1321 acggggtcct accaccaccc cgggagtcac atactgccgt ggtctacacc gaaaaagaca
     1381 ataagaagtc caagctggtg atctacggcg ggatgagtgg ctgcaggctg ggggacctgt
     1441 ggaccctaga tattgacacc ctgacgtgga ataagcccag tctcagcggg gtggcgcctc
     1501 ttcctcgcag tctccactcg gcaaccacca tcggaaataa aatgtacgtg tttggtggct
     1561 gggtgcctct cgtcatggat gacgtcaaag tggccacaca cgagaaggag tggaagtgta
     1621 ccaacacgct ggcttgtctc aacctggata ccatggcctg ggagaccatc ctgatggata
     1681 cactggagga caacatcccc cgtgctcggg ctggccactg cgcagtcgcc atcaacaccc
     1741 gcctgtacat ttggagtggg cgtgacggct accgcaaggc ctggaacaac caggtctgct
     1801 gcaaggacct ctggtaccta gagacagaaa agccaccacc cccagcccga gtacaactgg
     1861 tacgcgccaa caccaactcc ctggaggtga gctggggggc agtggcaaca gccgacagct
     1921 accttctcca gctccagaaa tatgacattc ctgccacggc tgctactgcc acctccccta
     1981 cacccaatcc ggtcccatct gtgcctgcca accctcccaa gagccctgcc ccagcagcag
     2041 ccgcacctgc tgtgcagccg ctgacccaag taggcatcac gctcctgccc caggctgccc
     2101 ccgcaccccc gaccaccacc accatccagg tcttgccaac ggtgcctggc agctccattt
     2161 ctgtgcccac cgcagccagg actcaaggtg tccctgctgt tctcaaagtg accggtcctc
     2221 aggctacaac aggaactcca ttggtcacca tgcgacctgc cagccaggct gggaaagccc
     2281 ctgtcaccgt gacctccctt cccgccggag tgcggatggt tgtgccaaca cagagtgccc
     2341 agggaacggt gattggcagt agcccacaga tgagtgggat ggccgcactg gccgctgcgg
     2401 ccgctgccac ccagaagatc cccccttcct cggcacccac ggtgctgagt gtcccagcgg
     2461 gtaccaccat cgtgaagacc atggctgtga cacctggcac taccaccctc ccagccactg
     2521 tgaaggtggc ctcctcgcca gtcatggtga gcaaccctgc cactcgcatg ctgaagactg
     2581 cagccgccca ggtggggaca tcggtttcct ccgccaccaa cacgtctacc cgccctatca
     2641 tcacagtgca caagtcaggc actgtgacag tggcccagca agcccaggtg gtgaccacag
     2701 ttgtgggcgg ggtcaccaag accatcaccc tggtgaagag ccccatctct gtcccaggag
     2761 gcagtgctct gatttccaat ctgggcaaag tgatgtcggt ggtccagacc aaaccagttc
     2821 agacttcagc agtcacaggc caggcgtcca cgggtcctgt gactcagatc atccagacca
     2881 aagggcccct gccagcggga acaatcctga agctggtgac ctcagcagat ggcaagccca
     2941 ccaccatcat cactaccacg caggccagtg gggcggggac caagcccacc atcctgggca
     3001 tcagcagcgt ctcccccagt accaccaagc ccggcacgac caccatcatc aaaaccatcc
     3061 ccatgtcggc catcatcacc caggcgggcg ccacgggtgt gaccagcagt cctggcatca
     3121 agtcccccat caccatcatc accaccaagg tgatgacttc aggaactgga gcacctgcga
     3181 aaatcatcac tgctgtcccc aaaattgcca ctggccacgg gcagcaggga gtgacccagg
     3241 tggtgcttaa gggggccccg ggacagccag gcaccatcct ccgcactgtg cccatggggg
     3301 gtgttcgcct ggtcacaccc gtcaccgtct ccgccgtcaa gccagccgtc accacgttgg
     3361 ttgtgaaagg caccacaggt gtcacgaccc taggcacagt gacaggcacc gtctccacca
     3421 gccttgccgg ggcggggggc cacagcacta gtgcttccct ggccacgccc atcaccacct
     3481 tgggcaccat tgccaccctc tcaagccagg tgatcaaccc cactgccatc actgtgtcgg
     3541 ccgcacagac cacgctgaca gcggcaggcg ggctcacaac cccaaccatc accatgcagc
     3601 ccgtgtccca gcccacccag gtaactctga tcacggcacc tagtggggtg gaggcccagc
     3661 ctgtgcatga cctccctgtg tccattctgg cctccccgac tacagaacag cccaccgcca
     3721 cagttaccat cgccgactca ggccagggtg atgtgcagcc tggcactgtc accttgagat
     3781 cttgaggatc ctgagaactt cagggtgagt ttggggaccc ttgattgttc tttctttttc
     3841 gctattgtaa aattcatgtt atatggaggg ggcaaagttt tcagggtgtt gtttagaatg
     3901 ggaagatgtc ccttgtatca ccatggaccc tcatgataat tttgtttctt tcactttcta
     3961 ctctgttgac aaccattgtc tcctcttatt ttcttttcat tttctgtaac tttttcgtta
     4021 aactttagct tgcatttgta acgaattttt aaattcactt ttgtttattt gtcagattgt
     4081 aagtactttc tctaatcact tttttttcaa ggcaatcagg gtatattata ttgtacttca
     4141 gcacagtttt agagaacaat tgttataatt aaatgataag gtagaatatt tctgcatata
     4201 aattctggct ggcgtggaaa tattcttatt ggtagaaaca actacaccct ggtcatcatc
     4261 ctgcctttct ctttatggtt acaatgatat acactgtttg agatgaggat aaaatactct
     4321 gagtccaaac cgggcccctc tgctaaccat gttcatgcct tcttctcttt cctacagctc
     4381 ctgggcaacg tgctggttgt tgtgctgtct catcattttg gcaaagaatt cactcctcag
     4441 gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca atgccctggc tcacaaatac
     4501 cactgagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat
     4561 ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg
     4621 tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat
     4681 ttggtttaga gtttggcaac atatgcccat atgctggctg ccatgaacaa aggttggcta
     4741 taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa
     4801 gccttgactt gaggttagat tttttttata ttttgttttg tgttattttt ttctttaaca
     4861 tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc
     4921 ccagtcatag ctgtccctct tctcttatgg agatccctcg aggagctttt tgcaaaagcc
     4981 taggcctcca aaaaagcctc ttcactactt ctggaatagc tcagaggccg aggcggcctc
     5041 ggcctctgca taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg
     5101 cggagttagg ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgct
     5161 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     5221 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     5281 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     5341 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     5401 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     5461 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     5521 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     5581 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     5641 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     5701 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     5761 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     5821 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     5881 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     5941 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     6001 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     6061 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     6121 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     6181 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     6241 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     6301 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     6361 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     6421 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     6481 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     6541 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     6601 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     6661 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     6721 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     6781 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     6841 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     6901 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     6961 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     7021 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     7081 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     7141 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     7201 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     7261 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     7321 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     7381 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     7441 ccgcatcagg aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     7501 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     7561 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     7621 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     7681 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     7741 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     7801 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     7861 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccat tcgccattca
     7921 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
     7981 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
     8041 gacgttgtaa aacgacggcc agtgaattgt aatacgactc actatagggc gaattcgagc
     8101 tcgcccc
//
