LOCUS       sequence-193879-       15134 bp    DNA     circular SYN 15-APR-2025
DEFINITION  First-generation rabies viral vector genome.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15134)
  AUTHORS   Chatterjee S, Sullivan HA, MacLennan BJ, Xu R, Hou Y, Lavin TK, Lea 
            NE, Michalski JE, Babcock KR, Dietrich S, Matthews GA, Beyeler A, 
            Calhoon GG, Glober G, Whitesell JD, Yao S, Cetin A, Harris JA, Zeng 
            H, Tye KM, Reid RC, Wickersham IR
  TITLE     Nontoxic, double-deletion-mutant rabies viral vectors for retrograde
            targeting of projection neurons.
  JOURNAL   Nat Neurosci. 2018 Apr;21(4):638-646. doi: 
            10.1038/s41593-018-0091-7. Epub 2018 Mar 5.
  PUBMED    29507411
REFERENCE   2  (bases 1 to 15134)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41593-018-0091-7"; journalName: "Nat Neurosci"; date: 
            "2018-04"; volume: "21"; issue: "4"; pages: "638-646"
COMMENT     Sequence Label: pRVdG-4GCaMP6f
FEATURES             Location/Qualifiers
     source          1..15134
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      2..458
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(89..108)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     299..320
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     584..606
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     598..615
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     599..615
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        657..1036
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1037..1240
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1191..1211
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1285..1304
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1285..1303
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4688..4697
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4694..4702,4703..4720,4721..4723,4724..4756,4757..4759,
                     4760..4783,4784..4810,4811..4867,4868..4873,4874..5143,
                     5144..5161,5162..5596,5597..5602,5603..6046)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6f
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEEFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFGVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      4790..4799
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     5080..5101
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5207..5228)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5468..5487)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             7797..7808
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(12807..12825)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      12821..12868
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(12891..12907)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12891..12907)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(12944..12964)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(12944..12962)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(12983..12999)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12983..12999)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12996..13018)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13007..13023
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13031..13037,13038..13055,13056..13061))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13076..13097
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13214..13231)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13385..13973)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13465..13484)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14144..14935,14936..15004))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGASKGGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14767..14786
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15005..15109)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
       61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      121 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      181 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
      241 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
      301 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
      361 taagggattt tgccgatttc ggcctattgg ttaaaaaaat gagctgattt aacaaaaatt
      421 taacgcgaat tttaacaaaa tattaacgct tacaatttcc attcgccatt caggctgcgc
      481 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
      541 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
      601 aaaacgacgg ccagtgagcg cgcgataagc ttgatatcga attcctgcag cgcgttgaca
      661 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      721 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      781 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      841 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      901 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      961 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     1021 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
     1081 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
     1141 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
     1201 cggtaggcgt gtacggtggg aggtctatat aagcagagct ctctggctaa ctagagaacc
     1261 cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc aagctggcta
     1321 gattaagcgt ctgatgagtc cgtgaggacg aaacccggcg taccgggtca cgcttaacaa
     1381 ccagatcaaa gaaaaaacag acattgtcaa ttgcaaagca aaaatgtaac acccctacaa
     1441 tggatgccga caagattgta ttcaaagtca ataatcaggt ggtctctttg aagcctgaga
     1501 ttatcgtgga tcaatatgag tacaagtacc ctgccatcaa agatttgaaa aagccctgta
     1561 taaccctagg aaaggctccc gatttaaata aagcatacaa gtcagttttg tcaggcatga
     1621 gcgccgccaa acttaatcct gacgatgtat gttcctattt ggcagcggca atgcagtttt
     1681 ttgaggggac atgtccggaa gactggacca gctatggaat tgtgattgca cgaaaaggag
     1741 ataagatcac cccaggttct ctggtggaga taaaacgtac tgatgtagaa gggaattggg
     1801 ctctgacagg aggcatggaa ctgacaagag accccactgt ccctgagcat gcgtccttag
     1861 tcggtcttct cttgagtctg tataggttga gcaaaatatc cgggcaaaac actggtaact
     1921 ataagacaaa cattgcagac aggatagagc agatttttga gacagcccct tttgttaaaa
     1981 tcgtggaaca ccatactcta atgacaactc acaaaatgtg tgctaattgg agtactatac
     2041 caaacttcag atttttggcc ggaacctatg acatgttttt ctcccggatt gagcatctat
     2101 attcagcaat cagagtgggc acagttgtca ctgcttatga agactgttca ggactggtat
     2161 catttactgg gttcataaaa caaatcaatc tcaccgctag agaggcaata ctatatttct
     2221 tccacaagaa ctttgaggaa gagataagaa gaatgtttga gccagggcag gagacagctg
     2281 ttcctcactc ttatttcatc cacttccgtt cactaggctt gagtgggaaa tctccttatt
     2341 catcaaatgc tgttggtcac gtgttcaatc tcattcactt tgtaggatgc tatatgggtc
     2401 aagtcagatc cctaaatgca acggttattg ctgcatgtgc tcctcatgaa atgtctgttc
     2461 tagggggcta tctgggagag gaattcttcg ggaaagggac atttgaaaga agattcttca
     2521 gagatgagaa agaacttcaa gaatacgagg cggctgaact gacaaagact gacgtagcac
     2581 tggcagatga tggaactgtc aactctgacg acgaggacta cttttcaggt gaaaccagaa
     2641 gtccggaggc tgtttatact cgaatcatga tgaatggagg tcgactaaag agatctcaca
     2701 tacggagata tgtctcagtc agttccaatc atcaagcccg tccaaactca ttcgccgagt
     2761 ttctaaacaa gacatattcg agtgactcat aagaagttga ataacaaaat gccggaaatc
     2821 tacggattgt gtatatccat catgaaaaaa actaacaccc ctcctttcga accatcccaa
     2881 acatgagcaa gatctttgtc aatcctagtg ctattagagc cggtctggcc gatcttgaga
     2941 tggctgaaga aactgttgat ctgatcaata gaaatatcga agacaatcag gctcatctcc
     3001 aaggggaacc catagaggtg gacaatctcc ctgaggatat ggggcgactt cacctggatg
     3061 atggaaaatc gcccaaccat ggtgagatag ccaaggtggg agaaggcaag tatcgagagg
     3121 actttcagat ggatgaagga gaggatccta gcttcctgtt ccagtcatac ctggaaaatg
     3181 ttggagtcca aatagtcaga caaatgaggt caggagagag atttctcaag atatggtcac
     3241 agaccgtaga agagattata tcctatgtcg cggtcaactt tcccaaccct ccaggaaagt
     3301 cttcagagga taaatcaacc cagactactg gccgagagct caagaaggag acaacaccca
     3361 ctccttctca gagagaaagc caatcatcga aagccaggat ggcggctcaa attgcttctg
     3421 gccctccagc ccttgaatgg tcggctacca atgaagagga tgatctatca gtggaggctg
     3481 agatcgctca ccagattgca gaaagtttct ccaaaaaata taagtttccc tctcgatcct
     3541 cagggatact cttgtataat tttgagcaat tgaaaatgaa ccttgatgat atagttaaag
     3601 aggcaaaaaa tgtaccaggt gtgacccgtt tagcccatga cgggtccaaa ctccccctaa
     3661 gatgtgtact gggatgggtc gctttggcca actctaagaa attccagttg ttagtcgaat
     3721 ccgacaagct gagtaaaatc atgcaagatg acttgaatcg ctatacatct tgctaaccga
     3781 acctctcccc tcagtccctc tagacaataa aatccgagat gtcccaaagt caacatgaaa
     3841 aaaacaggca acaccactga taaaatgaac ctcctacgta agatagtgaa aaaccgcagg
     3901 gacgaggaca ctcaaaaatc ctctcccgcg tcagcccctc tggatgacga tgacttgtgg
     3961 cttccacccc ctgaatacgt cccgctgaaa gaacttacag gcaagaagaa catgaggaac
     4021 ttttgtatca acggaagggt taaagtgtgt agcccgaatg gttactcgtt caggatcctg
     4081 cggcacattc tgaaatcatt cgacgagata tattctggga atcataggat gatcgggtta
     4141 gtcaaagtgg ttattggact ggctttgtca ggatctccag tccctgaggg cctgaactgg
     4201 gtatacaaat tgaggagaac ctttatcttc cagtgggctg attccagggg ccctcttgaa
     4261 ggggaggagt tggaatactc tcaggagatc acttgggatg atgatactga gttcgtcgga
     4321 ttgcaaataa gagtgattgc aaaacagtgt catatccagg gcagagtctg gtgtatcaac
     4381 atgaacccga gagcatgtca actatggtct gacatgtctc ttcagacaca aaggtccgaa
     4441 gaggacaaag attcctctct gcttctagaa taatcagatt atatcccgca aatttatcac
     4501 ttgtttacct ctggaggaga gaacatatgg gctcaactcc aacccttggg agcaatataa
     4561 caaaaaacat gttatggtgc cattaaaccg ctgcatttca tcaaagtcaa gttgattacc
     4621 tttacatttt gatcctcttg gatgtgaaaa aaactattaa catccctcaa aagaccccgg
     4681 gaaagccgcc accatgggtt ctcatcatca tcatcatcat ggtatggcta gcatgactgg
     4741 tggacagcaa atgggtcggg atctgtacga cgatgacgat aaggatctcg ccaccatggt
     4801 cgactcatca cgtcgtaagt ggaataagac aggtcacgca gtcagagcta taggtcggct
     4861 gagctcactc gagaacgtct atatcaaggc cgacaagcag aagaacggca tcaaggcgaa
     4921 cttcaagatc cgccacaaca tcgaggacgg cggcgtgcag ctcgcctacc actaccagca
     4981 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcgtgca
     5041 gtccaaactt tcgaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     5101 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagggcggta ccggagggag
     5161 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     5221 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggtgagggcg atgccaccta
     5281 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     5341 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     5401 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac atccaggagc gcaccatctt
     5461 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     5521 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     5581 caagctggag tacaacctgc cggaccaact gactgaagag cagatcgcag aatttaaaga
     5641 ggaattctcc ctatttgaca aggacgggga tgggacaata acaaccaagg agctggggac
     5701 ggtgatgcgg tctctggggc agaaccccac agaagcagag ctgcaggaca tgatcaatga
     5761 agtagatgcc gacggtgacg gcacaatcga cttccctgag ttcctgacaa tgatggcaag
     5821 aaaaatgaaa tacagggaca cggaagaaga aattagagaa gcgttcggtg tgtttgataa
     5881 ggatggcaat ggctacatca gtgcagcaga gcttcgccac gtgatgacaa accttggaga
     5941 gaagttaaca gatgaagagg ttgatgaaat gatcagggaa gcagacatcg atggggatgg
     6001 tcaggtaaac tacgaagagt ttgtacaaat gatgacagcg aagtgatagc tagcttataa
     6061 agtgctgggt catctaagct tttcagtcga gaaaaaaaca ttagatcaga agaacaactg
     6121 gcaacacttc tcaacctgag acttacttca agatgctcga tcctggagag gtctatgatg
     6181 accctattga cccaatcgag ttagaggctg aacccagagg aacccccatt gtccccaaca
     6241 tcttgaggaa ctctgactac aatctcaact ctcctttgat agaagatcct gctagactaa
     6301 tgttagaatg gttaaaaaca gggaatagac cttatcggat gactctaaca gacaattgct
     6361 ccaggtcttt cagagttttg aaagattatt tcaagaaggt agatttgggt tctctcaagg
     6421 tgggcggaat ggctgcacag tcaatgattt ctctctggtt atatggtgcc cactctgaat
     6481 ccaacaggag ccggagatgt ataacagact tggcccattt ctattccaag tcgtccccca
     6541 tagagaagct gttgaatctc acgctaggaa atagagggct gagaatcccc ccagagggag
     6601 tgttaagttg ccttgagagg gttgattatg ataatgcatt tggaaggtat cttgccaaca
     6661 cgtattcctc ttacttgttc ttccatgtaa tcaccttata catgaacgcc ctagactggg
     6721 atgaagaaaa gaccatccta gcattatgga aagatttaac ctcagtggac atcgggaagg
     6781 acttggtaaa gttcaaagac caaatatggg gactgctgat cgtgacaaag gactttgttt
     6841 actcccaaag ttccaattgt ctttttgaca gaaactacac acttatgcta aaagatcttt
     6901 tcttgtctcg cttcaactcc ttaatggtct tgctctctcc cccagagccc cgatactcag
     6961 atgacttgat atctcaacta tgccagctgt acattgctgg ggatcaagtc ttgtctatgt
     7021 gtggaaactc cggctatgaa gtcatcaaaa tattggagcc atatgtcgtg aatagtttag
     7081 tccagagagc agaaaagttt aggcctctca ttcattcctt gggagacttt cctgtattta
     7141 taaaagacaa ggtaagtcaa cttgaagaga cgttcggtcc ctgtgcaaga aggttcttta
     7201 gggctctgga tcaattcgac aacatacatg acttggtttt tgtgtttggc tgttacaggc
     7261 attgggggca cccatatata gattatcgaa agggtctgtc aaaactatat gatcaggttc
     7321 accttaaaaa aatgatagat aagtcctacc aggagtgctt agcaagcgac ctagccagga
     7381 ggatccttag atggggtttt gataagtact ccaagtggta tctggattca agattcctag
     7441 cccgagacca ccccttgact ccttatatca aaacccaaac atggccaccc aaacatattg
     7501 tagacttggt gggggataca tggcacaagc tcccgatcac gcagatcttt gagattcctg
     7561 aatcaatgga tccgtcagaa atattggatg acaaatcaca ttctttcacc agaacgagac
     7621 tagcttcttg gctgtcagaa aaccgagggg ggcctgttcc tagcgaaaaa gttattatca
     7681 cggccctgtc taagccgcct gtcaatcccc gagagtttct gaggtctata gacctcggag
     7741 gattgccaga tgaagacttg ataattggcc tcaagccaaa ggaacgggaa ttgaagattg
     7801 aaggtcgatt ctttgctcta atgtcatgga atctaagatt gtattttgtc atcactgaaa
     7861 aactcttggc caactacatc ttgccacttt ttgacgcgct gactatgaca gacaacctga
     7921 acaaggtgtt taaaaagctg atcgacaggg tcaccgggca agggcttttg gactattcaa
     7981 gggtcacata tgcatttcac ctggactatg aaaagtggaa caaccatcaa agattagagt
     8041 caacagagga tgtattttct gtcctagatc aagtgtttgg attgaagaga gtgttttcta
     8101 gaacacacga gttttttcaa aaggcctgga tctattattc agacagatca gacctcatcg
     8161 ggttacggga ggatcaaata tactgcttag atgcgtccaa cggcccaacc tgttggaatg
     8221 gccaggatgg cgggctagaa ggcttacggc agaagggctg gagtctagtc agcttattga
     8281 tgatagatag agaatctcaa atcaggaaca caagaaccaa aatactagct caaggagaca
     8341 accaggtttt atgtccgaca tacatgttgt cgccagggct atctcaagag gggctcctct
     8401 atgaattgga gagaatatca aggaatgcac tttcgatata cagagccgtc gaggaagggg
     8461 catctaagct agggctgatc atcaagaaag aagagaccat gtgtagttat gacttcctca
     8521 tctatggaaa aacccctttg tttagaggta acatattggt gcctgagtcc aaaagatggg
     8581 ccagagtctc ttgcgtctct aatgaccaaa tagtcaacct cgccaatata atgtcgacag
     8641 tgtccaccaa tgcgctaaca gtggcacaac actctcaatc tttgatcaaa ccgatgaggg
     8701 attttctgct catgtcagta caggcagtct ttcactacct gctatttagc ccaatcttaa
     8761 agggaagagt ttacaagatt ctgagcgctg aaggggagag ctttctccta gccatgtcaa
     8821 ggataatcta tctagatcct tctttgggag ggatatctgg aatgtccctc ggaagattcc
     8881 atatacgaca gttctcagac cctgtctctg aagggttatc cttctggaga gagatctggt
     8941 taagctccca agagtcctgg attcacgcgt tgtgtcaaga ggctggaaac ccagatcttg
     9001 gagagagaac actcgagagc ttcactcgcc ttctagaaga tccgaccacc ttaaatatca
     9061 gaggaggggc cagtcctacc attctactca aggatgcaat cagaaaggct ttatatgacg
     9121 aggtggacaa ggtggaaaat tcagagtttc gagaggcaat cctgttgtcc aagacccata
     9181 gagataattt tatactcttc ttaatatctg ttgagcctct gtttcctcga tttctcagtg
     9241 agctattcag ttcgtctttt ttgggaatcc ccgagtcaat cattggattg atacaaaact
     9301 cccgaacgat aagaaggcag tttagaaaga gtctctcaaa aactttagaa gaatccttct
     9361 acaactcaga gatccacggg attagtcgga tgacccagac acctcagagg gttggggggg
     9421 tgtggccttg ctcttcagag agggcagatc tacttaggga gatctcttgg ggaagaaaag
     9481 tggtaggcac gacagttcct cacccttctg agatgttggg attacttccc aagtcctcta
     9541 tttcttgcac ttgtggagca acaggaggag gcaatcctag agtttctgta tcagtactcc
     9601 cgtcctttga tcagtcattt ttttcacgag gccccctaaa gggatacttg ggctcgtcca
     9661 cctctatgtc gacccagcta ttccatgcat gggaaaaagt cactaatgtt catgtggtga
     9721 agagagctct atcgttaaaa gaatctataa actggttcat tactagagat tccaacttgg
     9781 ctcaagctct aattaggaac attatgtctc tgacaggccc tgatttccct ctagaggagg
     9841 cccctgtctt caaaaggacg gggtcagcct tgcataggtt caagtctgcc agatacagcg
     9901 aaggagggta ttcttctgtc tgcccgaacc tcctctctca tatttctgtt agtacagaca
     9961 ccatgtctga tttgacccaa gacgggaaga actacgattt catgttccag ccattgatgc
    10021 tttatgcaca gacatggaca tcagagctgg tacagagaga cacaaggcta agagactcta
    10081 cgtttcattg gcacctccga tgcaacaggt gtgtgagacc cattgacgac gtgaccctgg
    10141 agacctctca gatcttcgag tttccggatg tgtcgaaaag aatatccaga atggtttctg
    10201 gggctgtgcc tcacttccag aggcttcccg atatccgtct gagaccagga gattttgaat
    10261 ctctaagcgg tagagaaaag tctcaccata tcggatcagc tcaggggctc ttatactcaa
    10321 tcttagtggc aattcacgac tcaggataca atgatggaac catcttccct gtcaacatat
    10381 acggcaaggt ttcccctaga gactatttga gagggctcgc aaggggagta ttgataggat
    10441 cctcgatttg cttcttgaca agaatgacaa atatcaatat taatagacct cttgaattgg
    10501 tctcaggggt aatctcatat attctcctga ggctagataa ccatccctcc ttgtacataa
    10561 tgctcagaga accgtctctt agaggagaga tattttctat ccctcagaaa atccccgccg
    10621 cttatccaac cactatgaaa gaaggcaaca gatcaatctt gtgttatctc caacatgtgc
    10681 tacgctatga gcgagagata atcacggcgt ctccagagaa tgactggcta tggatctttt
    10741 cagactttag aagtgccaaa atgacgtacc tatccctcat tacttaccag tctcatcttc
    10801 tactccagag ggttgagaga aacctatcta agagtatgag agataacctg cgacaattga
    10861 gttctttgat gaggcaggtg ctgggcgggc acggagaaga taccttagag tcagacgaca
    10921 acattcaacg actgctaaaa gactctttac gaaggacaag atgggtggat caagaggtgc
    10981 gccatgcagc tagaaccatg actggagatt acagccccaa caagaaggtg tcccgtaagg
    11041 taggatgttc agaatgggtc tgctctgctc aacaggttgc agtctctacc tcagcaaacc
    11101 cggcccctgt ctcggagctt gacataaggg ccctctctaa gaggttccag aaccctttga
    11161 tctcgggctt gagagtggtt cagtgggcaa ccggtgctca ttataagctt aagcctattc
    11221 tagatgatct caatgttttc ccatctctct gccttgtagt tggggacggg tcagggggga
    11281 tatcaagggc agtcctcaac atgtttccag atgccaagct tgtgttcaac agtcttttag
    11341 aggtgaatga cctgatggct tccggaacac atccactgcc tccttcagca atcatgaggg
    11401 gaggaaatga tatcgtctcc agagtgatag atcttgactc aatctgggaa aaaccgtccg
    11461 acttgagaaa cttggcaacc tggaaatact tccagtcagt ccaaaagcag gtcaacatgt
    11521 cctatgacct cattatttgc gatgcagaag ttactgacat tgcatctatc aaccggatca
    11581 ccctgttaat gtccgatttt gcattgtcta tagatggacc actctatttg gtcttcaaaa
    11641 cttatgggac tatgctagta aatccaaact acaaggctat tcaacacctg tcaagagcgt
    11701 tcccctcggt cacagggttt atcacccaag taacttcgtc tttttcatct gagctctacc
    11761 tccgattctc caaacgaggg aagtttttca gagatgctga gtacttgacc tcttccaccc
    11821 ttcgagaaat gagccttgtg ttattcaatt gtagcagccc caagagtgag atgcagagag
    11881 ctcgttcctt gaactatcag gatcttgtga gaggatttcc tgaagaaatc atatcaaatc
    11941 cttacaatga gatgatcata actctgattg acagtgatgt agaatctttt ctagtccaca
    12001 agatggttga tgatcttgag ttacagaggg gaactctgtc taaagtggct atcattatag
    12061 ccatcatgat agttttctcc aacagagtct tcaacgtttc caaaccccta actgacccct
    12121 cgttctatcc accgtctgat cccaaaatcc tgaggcactt caacatatgt tgcagtacta
    12181 tgatgtatct atctactgct ttaggtgacg tccctagctt cgcaagactt cacgacctgt
    12241 ataacagacc tataacttat tacttcagaa agcaagtcat tcgagggaac gtttatctat
    12301 cttggagttg gtccaacgac acctcagtgt tcaaaagggt agcctgtaat tctagcctga
    12361 gtctgtcatc tcactggatc aggttgattt acaagatagt gaagactacc agactcgttg
    12421 gcagcatcaa ggatctatcc agagaagtgg aaagacacct tcataggtac aacaggtgga
    12481 tcaccctaga ggatatcaga tctagatcat ccctactaga ctacagttgc ctgtgaaccg
    12541 gatactcctg gaagcctgcc catgctaaga ctcttgtgtg atgtatcttg aaaaaaacaa
    12601 gatcctaaat ctgaaccttt ggttgtttga ttgtttttct catttttgtt gtttatttgt
    12661 taagcgtggg tcggcatggc atctccacct cctcgcggtc cgacctgggc atccgaagga
    12721 ggacgcacgt ccactcggat ggctaaggga gggcggggat ccggctgcta acaaagcccg
    12781 aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc
    12841 ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatccactag
    12901 ttctagagcg gccgccaccg cggtggagct ccagcttttg ttccctttag tgagggttaa
    12961 ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
    13021 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
    13081 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
    13141 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
    13201 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
    13261 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
    13321 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
    13381 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
    13441 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
    13501 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
    13561 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
    13621 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
    13681 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
    13741 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
    13801 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
    13861 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
    13921 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
    13981 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
    14041 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
    14101 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
    14161 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
    14221 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
    14281 cgcgagaccc acccttggag gctccagatt tatcagcaat aaaccagcca gccggaaggg
    14341 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
    14401 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
    14461 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
    14521 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
    14581 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
    14641 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
    14701 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
    14761 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
    14821 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
    14881 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
    14941 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
    15001 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
    15061 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
    15121 gaaaagtgcc acct
//
