LOCUS       sequence-194312-       12535 bp    DNA     circular SYN 14-APR-2025
DEFINITION  2xTet Operator inserted between NFkB and Sp1 sites in U3 of HIV-1 
            delta tat with 5' CMV-R-U5. 5' and 3' TAR elements were mutated to: 
            5'-GGTCTCTCTGGTTAGACCAGAAAGGAGCATTGGAGCTCTCTGGCTAACTAGGGAACCC-3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12535)
  AUTHORS   McCauley SM, Kim K, Nowosielska A, Dauphin A, Yurkovetskiy L, Diehl 
            WE, Luban J
  TITLE     Intron-containing RNA from the HIV-1 provirus activates type I 
            interferon and inflammatory cytokines.
  JOURNAL   Nat Commun. 2018 Dec 13;9(1):5305. doi: 10.1038/s41467-018-07753-2.
  PUBMED    30546110
REFERENCE   2  (bases 1 to 12535)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-12-13"; volume: "9(1):5305. doi"; pages: " 
            10.1038/s41467-018-07753-2"
COMMENT     Sequence Label: pBS NL4-3 envFS eGFP 5'CMV Delta-tat 
            Delta-TARx2_d2TetOp
FEATURES             Location/Qualifiers
     source          1..12535
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      7..435
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(94..113)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     304..325
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     enhancer        459..830
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        832..1030
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     985..1005
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             1049..1229
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1276..1401
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     CDS             1385..2887
                     /codon_start=1
                     /gene="gag"
                     /product="gag protein from human immunodeficiency virus 1"
                     /label=HIV-1 gag
                     /translation="MGARASVLSGGELDKWEKIRLRPGGKKQYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTIAVLYCVHQRIDVKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGNNSQVSQNYPIVQNLQGQMVHQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPVHAGP
                     IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
                     ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
                     ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
                     HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
                     PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
     CDS             2680..5691
                     /codon_start=1
                     /gene="pol"
                     /product="pol protein from human immunodeficiency virus 1"
                     /label=HIV-1 pol
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
                     NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_feature    5376..5493
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    8356..8589
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8774..8818
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             9112..9138
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             join(9405..9407,9408..10121)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(9405..9407,9408..10118)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(9447..9468)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9708..9727)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     10055..10076
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    10394..10412
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10421..10439
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     LTR             10458..10715
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(10791..11379)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10871..10890)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11550..12341,12342..12410))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12173..12192
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12411..12515)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
       61 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
      121 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
      181 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
      241 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
      301 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
      361 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
      421 aatttaacgc gaattttaac aaaatatttt aattaattga cattgattat tgataatagt
      481 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
      541 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
      601 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
      661 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
      721 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
      781 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
      841 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
      901 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
      961 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
     1021 atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga aaggagcatt
     1081 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     1141 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     1201 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
     1261 gtaaagccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     1321 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
     1381 agagatgggt gcgagagcgt cggtattaag cgggggagaa ttagataaat gggaaaaaat
     1441 tcggttaagg ccagggggaa agaaacaata taaactaaaa catatagtat gggcaagcag
     1501 ggagctagaa cgattcgcag ttaatcctgg ccttttagag acatcagaag gctgtagaca
     1561 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     1621 taatacaata gcagtcctct attgtgtgca tcaaaggata gatgtaaaag acaccaagga
     1681 agccttagat aagatagagg aagagcaaaa caaaagtaag aaaaaggcac agcaagcagc
     1741 agctgacaca ggaaacaaca gccaggtcag ccaaaattac cctatagtgc agaacctcca
     1801 ggggcaaatg gtacatcagg ccatatcacc tagaacttta aatgcatggg taaaagtagt
     1861 agaagagaag gctttcagcc cagaagtaat acccatgttt tcagcattat cagaaggagc
     1921 caccccacaa gatttaaata ccatgctaaa cacagtgggg ggacatcaag cagccatgca
     1981 aatgttaaaa gagaccatca atgaggaagc tgcagaatgg gatagattgc atccagtgca
     2041 tgcagggcct attgcaccag gccagatgag agaaccaagg ggaagtgaca tagcaggaac
     2101 tactagtacc cttcaggaac aaataggatg gatgacacat aatccaccta tcccagtagg
     2161 agaaatctat aaaagatgga taatcctggg attaaataaa atagtaagaa tgtatagccc
     2221 taccagcatt ctggacataa gacaaggacc aaaggaaccc tttagagact atgtagaccg
     2281 attctataaa actctaagag ccgagcaagc ttcacaagag gtaaaaaatt ggatgacaga
     2341 aaccttgttg gtccaaaatg cgaacccaga ttgtaagact attttaaaag cattgggacc
     2401 aggagcgaca ctagaagaaa tgatgacagc atgtcaggga gtggggggac ccggccataa
     2461 agcaagagtt ttggctgaag caatgagcca agtaacaaat ccagctacca taatgataca
     2521 gaaaggcaat tttaggaacc aaagaaagac tgttaagtgt ttcaattgtg gcaaagaagg
     2581 gcacatagcc aaaaattgca gggcccctag gaaaaagggc tgttggaaat gtggaaagga
     2641 aggacaccaa atgaaagatt gtactgagag acaggctaat tttttaggga agatctggcc
     2701 ttcccacaag ggaaggccag ggaattttct tcagagcaga ccagagccaa cagccccacc
     2761 agaagagagc ttcaggtttg gggaagagac aacaactccc tctcagaagc aggagccgat
     2821 agacaaggaa ctgtatcctt tagcttccct cagatcactc tttggcagcg acccctcgtc
     2881 acaataaaga taggggggca attaaaggaa gctctattag atacaggagc agatgataca
     2941 gtattagaag aaatgaattt gccaggaaga tggaaaccaa aaatgatagg gggaattgga
     3001 ggttttatca aagtaagaca gtatgatcag atactcatag aaatctgcgg acataaagct
     3061 ataggtacag tattagtagg acctacacct gtcaacataa ttggaagaaa tctgttgact
     3121 cagattggct gcactttaaa ttttcccatt agtcctattg agactgtacc agtaaaatta
     3181 aagccaggaa tggatggccc aaaagttaaa caatggccat tgacagaaga aaaaataaaa
     3241 gcattagtag aaatttgtac agaaatggaa aaggaaggaa aaatttcaaa aattgggcct
     3301 gaaaatccat acaatactcc agtatttgcc ataaagaaaa aagacagtac taaatggaga
     3361 aaattagtag atttcagaga acttaataag agaactcaag atttctggga agttcaatta
     3421 ggaataccac atcctgcagg gttaaaacag aaaaaatcag taacagtact ggatgtgggc
     3481 gatgcatatt tttcagttcc cttagataaa gacttcagga agtatactgc atttaccata
     3541 cctagtataa acaatgagac accagggatt agatatcagt acaatgtgct tccacaggga
     3601 tggaaaggat caccagcaat attccagtgt agcatgacaa aaatcttaga gccttttaga
     3661 aaacaaaatc cagacatagt catctatcaa tacatggatg atttgtatgt aggatctgac
     3721 ttagaaatag ggcagcatag aacaaaaata gaggaactga gacaacatct gttgaggtgg
     3781 ggatttacca caccagacaa aaaacatcag aaagaacctc cattcctttg gatgggttat
     3841 gaactccatc ctgataaatg gacagtacag cctatagtgc tgccagaaaa ggacagctgg
     3901 actgtcaatg acatacagaa attagtggga aaattgaatt gggcaagtca gatttatgca
     3961 gggattaaag taaggcaatt atgtaaactt cttaggggaa ccaaagcact aacagaagta
     4021 gtaccactaa cagaagaagc agagctagaa ctggcagaaa acagggagat tctaaaagaa
     4081 ccggtacatg gagtgtatta tgacccatca aaagacttaa tagcagaaat acagaagcag
     4141 gggcaaggcc aatggacata tcaaatttat caagagccat ttaaaaatct gaaaacagga
     4201 aagtatgcaa gaatgaaggg tgcccacact aatgatgtga aacaattaac agaggcagta
     4261 caaaaaatag ccacagaaag catagtaata tggggaaaga ctcctaaatt taaattaccc
     4321 atacaaaagg aaacatggga agcatggtgg acagagtatt ggcaagccac ctggattcct
     4381 gagtgggagt ttgtcaatac ccctccctta gtgaagttat ggtaccagtt agagaaagaa
     4441 cccataatag gagcagaaac tttctatgta gatggggcag ccaataggga aactaaatta
     4501 ggaaaagcag gatatgtaac tgacagagga agacaaaaag ttgtccccct aacggacaca
     4561 acaaatcaga agactgagtt acaagcaatt catctagctt tgcaggattc gggattagaa
     4621 gtaaacatag tgacagactc acaatatgca ttgggaatca ttcaagcaca accagataag
     4681 agtgaatcag agttagtcag tcaaataata gagcagttaa taaaaaagga aaaagtctac
     4741 ctggcatggg taccagcaca caaaggaatt ggaggaaatg aacaagtaga taaattggtc
     4801 agtgctggaa tcaggaaagt actattttta gatggaatag ataaggccca agaagaacat
     4861 gagaaatatc acagtaattg gagagcaatg gctagtgatt ttaacctacc acctgtagta
     4921 gcaaaagaaa tagtagccag ctgtgataaa tgtcagctaa aaggggaagc catgcatgga
     4981 caagtagact gtagcccagg aatatggcag ctagattgta cacatttaga aggaaaagtt
     5041 atcttggtag cagttcatgt agccagtgga tatatagaag cagaagtaat tccagcagag
     5101 acagggcaag aaacagcata cttcctctta aaattagcag gaagatggcc agtaaaaaca
     5161 gtacatacag acaatggcag caatttcacc agtactacag ttaaggccgc ctgttggtgg
     5221 gcggggatca agcaggaatt tggcattccc tacaatcccc aaagtcaagg agtaatagaa
     5281 tctatgaata aagaattaaa gaaaattata ggacaggtaa gagatcaggc tgaacatctt
     5341 aagacagcag tacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
     5401 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     5461 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
     5521 ccagtttgga aaggaccagc aaagctcctc tggaaaggtg aaggggcagt agtaatacaa
     5581 gataatagtg acataaaagt agtgccaaga agaaaagcaa agatcatcag ggattatgga
     5641 aaacagatgg caggtgatga ttgtgtggca agtagacagg atgaggatta acacatggaa
     5701 aagattagta aaacaccata tgtatatttc aaggaaagct aaggactggt tttatagaca
     5761 tcactatgaa agtactaatc caaaaataag ttcagaagta cacatcccac taggggatgc
     5821 taaattagta ataacaacat attggggtct gcatacagga gaaagagact ggcatttggg
     5881 tcagggagtc tccatagaat ggaggaaaaa gagatatagc acacaagtag accctgacct
     5941 agcagaccaa ctaattcatc tgcactattt tgattgtttt tcagaatctg ctataagaaa
     6001 taccatatta ggacgtatag ttagtcctag gtgtgaatat caagcaggac ataacaaggt
     6061 aggatctcta cagtacttgg cactagcagc attaataaaa ccaaaacaga taaagccacc
     6121 tttgcctagt gttaggaaac tgacagagga cagatggaac aagccccaga agaccaaggg
     6181 ccacagaggg agccatacaa tgaatggaca ctagagcttt tagaggaact taagagtgaa
     6241 gctgttagac attttcctag gatatggctc cataacttag gacaacatat ctatgaaact
     6301 tacggggata cttgggcagg agtggaagcc ataataagaa ttctgcaaca actgctgttt
     6361 atccatttca gaattgggtg tcgacatagc agaataggcg ttactcgaca gaggagagca
     6421 agaaacggag ccagtagatc ctagactaga gccctggaag catccaggaa gtcagcctaa
     6481 aactgcttgt accaattgct agtgtaaaaa gtgttgcttt cattgccaag tttgtttcac
     6541 gacaaaagcc ttaggcatct cctatggcag gaagaagcgg agacagcgac gaagagctca
     6601 tcagaacagt cagactcatc aagcttctct atcaaagcag taagtagtac atgtaatgca
     6661 acctataata gtagcaatag tagcattagt agtagcaata ataatagcaa tagttgtgtg
     6721 gtccatagta atcatagaat ataggaaaat attaagacaa agaaaaatag acaggttaat
     6781 tgatagacta atagaaagag cagaagacag tggcaatgag agtgaaggag aagtatcagc
     6841 acttgtggag atgggggtgg aaatggggca ccatgctcct tgggatattg atgatctgta
     6901 gtgctacaga aaaattgtgg gtcaccgtct attatggggt acctgtgtgg aaggaagcaa
     6961 ccaccactct attttgtgca tcagatgcta aagcatatat gatacagagg tacataatgt
     7021 ttgggccaca catgcctgtg tacccacaga ccccaaccca caagaagtag tattggtaaa
     7081 tgtgacagaa aattttaaca tgtggaaaaa tgacatggta gaacagatgc atgaggatat
     7141 aatcagttta tgggatcaaa gcctaaagcc atgtgtaaaa ttaaccccac tctgtgttag
     7201 tttaaagtgc actgatttga agaatgatac taataccaat agtagtagcg ggagaatgat
     7261 aatggagaaa ggagagataa aaaactgctc tttcaatatc agcacaagca taagagataa
     7321 ggtgcagaaa gaatatgcat tcttttataa acttgatata gtaccaatag ataataccag
     7381 ctataggttg ataagttgta acacctcagt cattacacag gcctgtccaa aggtatcctt
     7441 tgagccaatt cccatacatt attgtgcccc ggctggtttt gcgattctaa aatgtaataa
     7501 taagacgttc aatggaacag gaccatgtac aaatgtcagc acagtacaat gtacacatgg
     7561 aatcaggcca gtagtatcaa ctcaactgct gttaaatggc agtctagcag aagaagatgt
     7621 agtaattaga tctgccaatt tcacagacaa tgctaaaacc ataatagtac agctgaacac
     7681 atctgtagaa attaattgta caagacccaa caacaataca agaaaaagta tccgtatcca
     7741 gaggggacca gggagagcat ttgttacaat aggaaaaata ggaaatatga gacaagcaca
     7801 ttgtaacatt agtagagcaa aatggaatgc cactttaaaa cagatagcta gcaaattaag
     7861 agaacaattt ggaaataata aaacaataat ctttaagcaa tcctcaggag gggacccaga
     7921 aattgtaacg cacagtttta attgtggagg ggaatttttc tactgtaatt caacacaact
     7981 gtttaatagt acttggttta atagtacttg gagtactgaa gggtcaaata acactgaagg
     8041 aagtgacaca atcacactcc catgcagaat aaaacaattt ataaacatgt ggcaggaagt
     8101 aggaaaagca atgtatgccc ctcccatcag tggacaaatt agatgttcat caaatattac
     8161 tgggctgcta ttaacaagag atggtggtaa taacaacaat gggtccgaga tcttcagacc
     8221 tggaggaggc gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
     8281 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
     8341 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
     8401 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg atatagtgca
     8461 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
     8521 ctggggcatc aaacagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
     8581 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
     8641 gaatgctagt tggagtaata aatctctgga acagatttgg aataacatga cctggatgga
     8701 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
     8761 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
     8821 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
     8881 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
     8941 gggatattca ccattatcgt ttcagaccca cctcccaatc ccgaggggac ccgacaggcc
     9001 cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa
     9061 cggatcctta gcacttatct gggacgatct gcggagcctg tgcctcttca gctaccaccg
     9121 cttgagagac ttactcttga ttgtaacgag gattgtggaa cttctgggac gcagggggtg
     9181 ggaagccctc aaatattggt ggaatctcct acaatattgg agtcaggaac taaagaatag
     9241 tgctgttaac ttgctcaatg ccacagccat agcagtagct gaggggacag atagggttat
     9301 agaagtagta caaggagctt gtagagctat tcgccacata cctagaagaa taagacaggg
     9361 cttggaaagg attttgctat aagatgggtg gcgcggccgc tctggtgagc aagggcgagg
     9421 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     9481 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     9541 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     9601 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     9661 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     9721 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     9781 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     9841 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     9901 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     9961 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
    10021 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
    10081 ccgccgggat cactctcggc atggacgagc tgtacaagta actcgagccc tcaggtacct
    10141 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
    10201 ggactggaag ggctaattca ctcccaaaga agacaagata tccttgatct ggaagtgtta
    10261 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag
    10321 tacttcaaga actgctgaca tcgagcttgc tacaagggac tttccgctgg ggactttccg
    10381 tcgagtttac cactccctat cagtgataga gaaataccac tccctatcag tgatagagaa
    10441 aagtgaaagt cgacagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
    10501 tgctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagaaagg
    10561 agcattggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
    10621 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
    10681 cagacccttt tagtcagtgt ggaaaatctc tagcaggccg gcccaattga catgtgagca
    10741 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
    10801 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
    10861 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
    10921 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
    10981 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
    11041 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
    11101 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
    11161 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
    11221 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
    11281 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
    11341 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
    11401 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
    11461 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
    11521 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
    11581 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
    11641 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
    11701 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
    11761 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
    11821 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
    11881 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
    11941 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
    12001 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
    12061 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
    12121 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
    12181 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
    12241 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
    12301 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    12361 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    12421 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    12481 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgc
//
