LOCUS       sequence-194319-       14029 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Deletion from NL4-3 nt 6054-7489 encompassing all of vpu and env 
            until before the RRE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14029)
  AUTHORS   McCauley SM, Kim K, Nowosielska A, Dauphin A, Yurkovetskiy L, Diehl 
            WE, Luban J
  TITLE     Intron-containing RNA from the HIV-1 provirus activates type I 
            interferon and inflammatory cytokines.
  JOURNAL   Nat Commun. 2018 Dec 13;9(1):5305. doi: 10.1038/s41467-018-07753-2.
  PUBMED    30546110
REFERENCE   2  (bases 1 to 14029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-12-13"; volume: "9(1):5305. doi"; pages: " 
            10.1038/s41467-018-07753-2"
COMMENT     Sequence Label: pNL4-3 envFS eGFP Delta-vpu/env
FEATURES             Location/Qualifiers
     source          1..14029
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             377..634
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     CDS             790..2292
                     /codon_start=1
                     /gene="gag"
                     /product="gag protein from human immunodeficiency virus 1"
                     /label=HIV-1 gag
                     /translation="MGARASVLSGGELDKWEKIRLRPGGKKQYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTIAVLYCVHQRIDVKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGNNSQVSQNYPIVQNLQGQMVHQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPVHAGP
                     IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
                     ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
                     ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
                     HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
                     PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
     CDS             2085..5096
                     /codon_start=1
                     /gene="pol"
                     /product="pol protein from human immunodeficiency virus 1"
                     /label=HIV-1 pol
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
                     NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_feature    4781..4898
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    6325..6558
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6743..6787
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             7081..7107
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             join(7374..7376,7377..8090)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(7374..7376,7377..8087)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(7416..7437)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7677..7696)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8024..8045
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             8280..8913
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10553..10570)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10724..11312)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10804..10823)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11483..12274,12275..12343))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12106..12125
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12344..12448)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     12516..12534
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(12572..12594)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     12694..12713
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tggaagggct aatttggtcc caaaaaagac aagagatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc agatatccac
      121 tgacctttgg atggtgcttc aagttagtac cagttgaacc agagcaagta gaagaggcca
      181 atgaaggaga gaacaacagc ttgttacacc ctatgagcca gcatgggatg gaggacccgg
      241 agggagaagt attagtgtgg aagtttgaca gcctcctagc atttcgtcac atggcccgag
      301 agctgcatcc ggagtactac aaagactgct gacatcgagc tttctacaag ggactttccg
      361 ctggggactt tccagggagg tgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 gctacatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgctca aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagtaaa gccagaggag atctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcggta ttaagcgggg gagaattaga taaatgggaa
      841 aaaattcggt taaggccagg gggaaagaaa caatataaac taaaacatat agtatgggca
      901 agcagggagc tagaacgatt cgcagttaat cctggccttt tagagacatc agaaggctgt
      961 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     1021 ttatataata caatagcagt cctctattgt gtgcatcaaa ggatagatgt aaaagacacc
     1081 aaggaagcct tagataagat agaggaagag caaaacaaaa gtaagaaaaa ggcacagcaa
     1141 gcagcagctg acacaggaaa caacagccag gtcagccaaa attaccctat agtgcagaac
     1201 ctccaggggc aaatggtaca tcaggccata tcacctagaa ctttaaatgc atgggtaaaa
     1261 gtagtagaag agaaggcttt cagcccagaa gtaataccca tgttttcagc attatcagaa
     1321 ggagccaccc cacaagattt aaataccatg ctaaacacag tggggggaca tcaagcagcc
     1381 atgcaaatgt taaaagagac catcaatgag gaagctgcag aatgggatag attgcatcca
     1441 gtgcatgcag ggcctattgc accaggccag atgagagaac caaggggaag tgacatagca
     1501 ggaactacta gtacccttca ggaacaaata ggatggatga cacataatcc acctatccca
     1561 gtaggagaaa tctataaaag atggataatc ctgggattaa ataaaatagt aagaatgtat
     1621 agccctacca gcattctgga cataagacaa ggaccaaagg aaccctttag agactatgta
     1681 gaccgattct ataaaactct aagagccgag caagcttcac aagaggtaaa aaattggatg
     1741 acagaaacct tgttggtcca aaatgcgaac ccagattgta agactatttt aaaagcattg
     1801 ggaccaggag cgacactaga agaaatgatg acagcatgtc agggagtggg gggacccggc
     1861 cataaagcaa gagttttggc tgaagcaatg agccaagtaa caaatccagc taccataatg
     1921 atacagaaag gcaattttag gaaccaaaga aagactgtta agtgtttcaa ttgtggcaaa
     1981 gaagggcaca tagccaaaaa ttgcagggcc cctaggaaaa agggctgttg gaaatgtgga
     2041 aaggaaggac accaaatgaa agattgtact gagagacagg ctaatttttt agggaagatc
     2101 tggccttccc acaagggaag gccagggaat tttcttcaga gcagaccaga gccaacagcc
     2161 ccaccagaag agagcttcag gtttggggaa gagacaacaa ctccctctca gaagcaggag
     2221 ccgatagaca aggaactgta tcctttagct tccctcagat cactctttgg cagcgacccc
     2281 tcgtcacaat aaagataggg gggcaattaa aggaagctct attagataca ggagcagatg
     2341 atacagtatt agaagaaatg aatttgccag gaagatggaa accaaaaatg atagggggaa
     2401 ttggaggttt tatcaaagta agacagtatg atcagatact catagaaatc tgcggacata
     2461 aagctatagg tacagtatta gtaggaccta cacctgtcaa cataattgga agaaatctgt
     2521 tgactcagat tggctgcact ttaaattttc ccattagtcc tattgagact gtaccagtaa
     2581 aattaaagcc aggaatggat ggcccaaaag ttaaacaatg gccattgaca gaagaaaaaa
     2641 taaaagcatt agtagaaatt tgtacagaaa tggaaaagga aggaaaaatt tcaaaaattg
     2701 ggcctgaaaa tccatacaat actccagtat ttgccataaa gaaaaaagac agtactaaat
     2761 ggagaaaatt agtagatttc agagaactta ataagagaac tcaagatttc tgggaagttc
     2821 aattaggaat accacatcct gcagggttaa aacagaaaaa atcagtaaca gtactggatg
     2881 tgggcgatgc atatttttca gttcccttag ataaagactt caggaagtat actgcattta
     2941 ccatacctag tataaacaat gagacaccag ggattagata tcagtacaat gtgcttccac
     3001 agggatggaa aggatcacca gcaatattcc agtgtagcat gacaaaaatc ttagagcctt
     3061 ttagaaaaca aaatccagac atagtcatct atcaatacat ggatgatttg tatgtaggat
     3121 ctgacttaga aatagggcag catagaacaa aaatagagga actgagacaa catctgttga
     3181 ggtggggatt taccacacca gacaaaaaac atcagaaaga acctccattc ctttggatgg
     3241 gttatgaact ccatcctgat aaatggacag tacagcctat agtgctgcca gaaaaggaca
     3301 gctggactgt caatgacata cagaaattag tgggaaaatt gaattgggca agtcagattt
     3361 atgcagggat taaagtaagg caattatgta aacttcttag gggaaccaaa gcactaacag
     3421 aagtagtacc actaacagaa gaagcagagc tagaactggc agaaaacagg gagattctaa
     3481 aagaaccggt acatggagtg tattatgacc catcaaaaga cttaatagca gaaatacaga
     3541 agcaggggca aggccaatgg acatatcaaa tttatcaaga gccatttaaa aatctgaaaa
     3601 caggaaagta tgcaagaatg aagggtgccc acactaatga tgtgaaacaa ttaacagagg
     3661 cagtacaaaa aatagccaca gaaagcatag taatatgggg aaagactcct aaatttaaat
     3721 tacccataca aaaggaaaca tgggaagcat ggtggacaga gtattggcaa gccacctgga
     3781 ttcctgagtg ggagtttgtc aatacccctc ccttagtgaa gttatggtac cagttagaga
     3841 aagaacccat aataggagca gaaactttct atgtagatgg ggcagccaat agggaaacta
     3901 aattaggaaa agcaggatat gtaactgaca gaggaagaca aaaagttgtc cccctaacgg
     3961 acacaacaaa tcagaagact gagttacaag caattcatct agctttgcag gattcgggat
     4021 tagaagtaaa catagtgaca gactcacaat atgcattggg aatcattcaa gcacaaccag
     4081 ataagagtga atcagagtta gtcagtcaaa taatagagca gttaataaaa aaggaaaaag
     4141 tctacctggc atgggtacca gcacacaaag gaattggagg aaatgaacaa gtagataaat
     4201 tggtcagtgc tggaatcagg aaagtactat ttttagatgg aatagataag gcccaagaag
     4261 aacatgagaa atatcacagt aattggagag caatggctag tgattttaac ctaccacctg
     4321 tagtagcaaa agaaatagta gccagctgtg ataaatgtca gctaaaaggg gaagccatgc
     4381 atggacaagt agactgtagc ccaggaatat ggcagctaga ttgtacacat ttagaaggaa
     4441 aagttatctt ggtagcagtt catgtagcca gtggatatat agaagcagaa gtaattccag
     4501 cagagacagg gcaagaaaca gcatacttcc tcttaaaatt agcaggaaga tggccagtaa
     4561 aaacagtaca tacagacaat ggcagcaatt tcaccagtac tacagttaag gccgcctgtt
     4621 ggtgggcggg gatcaagcag gaatttggca ttccctacaa tccccaaagt caaggagtaa
     4681 tagaatctat gaataaagaa ttaaagaaaa ttataggaca ggtaagagat caggctgaac
     4741 atcttaagac agcagtacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     4801 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     4861 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     4921 gagatccagt ttggaaagga ccagcaaagc tcctctggaa aggtgaaggg gcagtagtaa
     4981 tacaagataa tagtgacata aaagtagtgc caagaagaaa agcaaagatc atcagggatt
     5041 atggaaaaca gatggcaggt gatgattgtg tggcaagtag acaggatgag gattaacaca
     5101 tggaaaagat tagtaaaaca ccatatgtat atttcaagga aagctaagga ctggttttat
     5161 agacatcact atgaaagtac taatccaaaa ataagttcag aagtacacat cccactaggg
     5221 gatgctaaat tagtaataac aacatattgg ggtctgcata caggagaaag agactggcat
     5281 ttgggtcagg gagtctccat agaatggagg aaaaagagat atagcacaca agtagaccct
     5341 gacctagcag accaactaat tcatctgcac tattttgatt gtttttcaga atctgctata
     5401 agaaatacca tattaggacg tatagttagt cctaggtgtg aatatcaagc aggacataac
     5461 aaggtaggat ctctacagta cttggcacta gcagcattaa taaaaccaaa acagataaag
     5521 ccacctttgc ctagtgttag gaaactgaca gaggacagat ggaacaagcc ccagaagacc
     5581 aagggccaca gagggagcca tacaatgaat ggacactaga gcttttagag gaacttaaga
     5641 gtgaagctgt tagacatttt cctaggatat ggctccataa cttaggacaa catatctatg
     5701 aaacttacgg ggatacttgg gcaggagtgg aagccataat aagaattctg caacaactgc
     5761 tgtttatcca tttcagaatt gggtgtcgac atagcagaat aggcgttact cgacagagga
     5821 gagcaagaaa tggagccagt agatcctaga ctagagccct ggaagcatcc aggaagtcag
     5881 cctaaaactg cttgtaccaa ttgctattgt aaaaagtgtt gctttcattg ccaagtttgt
     5941 ttcatgacaa aagccttagg catctcctat ggcaggaaga agcggagaca gcgacgaaga
     6001 gctcatcaga acagtcagac tcatcaagct tctctatcaa agcagtaagt agtacatgtg
     6061 gcaggaagta ggaaaagcaa tgtatgcccc tcccatcagt ggacaaatta gatgttcatc
     6121 aaatattact gggctgctat taacaagaga tggtggtaat aacaacaatg ggtccgagat
     6181 cttcagacct ggaggaggcg atatgaggga caattggaga agtgaattat ataaatataa
     6241 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     6301 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     6361 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctga
     6421 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     6481 actcacagtc tggggcatca aacagctcca ggcaagaatc ctggctgtgg aaagatacct
     6541 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     6601 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga ataacatgac
     6661 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     6721 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     6781 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     6841 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     6901 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaatcc cgaggggacc
     6961 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
     7021 attagtgaac ggatccttag cacttatctg ggacgatctg cggagcctgt gcctcttcag
     7081 ctaccaccgc ttgagagact tactcttgat tgtaacgagg attgtggaac ttctgggacg
     7141 cagggggtgg gaagccctca aatattggtg gaatctccta caatattgga gtcaggagct
     7201 aaagaatagt gctgttagct tgctcaatgc cacagccata gcagtagctg aggggacaga
     7261 tagggttata gaagtagtac aaggagcttg tagagctatt cgccacatac ctagaagaat
     7321 aagacagggc ttggaaagga ttttgctata agatgggtgg cgcggccgct ctggtgagca
     7381 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     7441 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     7501 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     7561 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     7621 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     7681 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     7741 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     7801 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     7861 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     7921 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     7981 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     8041 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa ctcgagacct
     8101 agaaaaacat ggagcaatca caagtagcaa tacagcagct aacaatgctg cttgtgcctg
     8161 gctagaagca caagaggagg aagaggtggg ttttccagtc acacctcagg tacctttaag
     8221 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     8281 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
     8341 acaaggctac ttccctgatt ggcagaacta cacaccaggg ccaggggtca gatatccact
     8401 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
     8461 taaaggagag aacaccagct tgttacaccc tgtgagcctg catggaatgg atgaccctga
     8521 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
     8581 gctgcatccg gagtacttca agaactgctg acatcgagct tgctacaagg gactttccgc
     8641 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatg
     8701 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
     8761 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     8821 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     8881 gaccctttta gtcagtgtgg aaaatctcta gcacccagga ggtagaggtt gcagtgagcc
     8941 aagatcgcgc cactgcattc cagcctgggc aagaaaacaa gactgtctaa aataataata
     9001 ataagttaag ggtattaaat atatttatac atggaggtca taaaaatata tatatttggg
     9061 ctgggcgcag tggctcacac ctgcgcccgg ccctttggga ggccgaggca ggtggatcac
     9121 ctgagtttgg gagttccaga ccagcctgac caacatggag aaaccccttc tctgtgtatt
     9181 tttagtagat tttattttat gtgtatttta ttcacaggta tttctggaaa actgaaactg
     9241 tttttcctct actctgatac cacaagaatc atcagcacag aggaagactt ctgtgatcaa
     9301 atgtggtggg agagggaggt tttcaccagc acatgagcag tcagttctgc cgcagactcg
     9361 gcgggtgtcc ttcggttcag ttccaacacc gcctgcctgg agagaggtca gaccacaggg
     9421 tgagggctca gtccccaaga cataaacacc caagacataa acacccaaca ggtccacccc
     9481 gcctgctgcc caggcagagc cgattcacca agacgggaat taggatagag aaagagtaag
     9541 tcacacagag ccggctgtgc gggagaacgg agttctatta tgactcaaat cagtctcccc
     9601 aagcattcgg ggatcagagt ttttaaggat aacttagtgt gtagggggcc agtgagttgg
     9661 agatgaaagc gtagggagtc gaaggtgtcc ttttgcgccg agtcagttcc tgggtggggg
     9721 ccacaagatc ggatgagcca gtttatcaat ccgggggtgc cagctgatcc atggagtgca
     9781 gggtctgcaa aatatctcaa gcactgattg atcttaggtt ttacaatagt gatgttaccc
     9841 caggaacaat ttggggaagg tcagaatctt gtagcctgta gctgcatgac tcctaaacca
     9901 taatttcttt tttgtttttt tttttttatt tttgagacag ggtctcactc tgtcacctag
     9961 gctggagtgc agtggtgcaa tcacagctca ctgcagcctc aacgtcgtaa gctcaagcga
    10021 tcctcccacc tcagcctgcc tggtagctga gactacaagc gacgccccag ttaatttttg
    10081 tatttttggt agaggcagcg ttttgccgtg tggccctggc tggtctcgaa ctcctgggct
    10141 caagtgatcc agcctcagcc tcccaaagtg ctgggacaac cggggccagt cactgcacct
    10201 ggccctaaac cataatttct aatcttttgg ctaatttgtt agtcctacaa aggcagtcta
    10261 gtccccaggc aaaaaggggg tttgtttcgg gaaagggctg ttactgtctt tgtttcaaac
    10321 tataaactaa gttcctccta aacttagttc ggcctacacc caggaatgaa caaggagagc
    10381 ttggaggtta gaagcacgat ggaattggtt aggtcagatc tctttcactg tctgagttat
    10441 aattttgcaa tggtggttca aagactgccc gcttctgaca ccagtcgctg cattaatgaa
    10501 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
    10561 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
    10621 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
    10681 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
    10741 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
    10801 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
    10861 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
    10921 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
    10981 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
    11041 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
    11101 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
    11161 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
    11221 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
    11281 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
    11341 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
    11401 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
    11461 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
    11521 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
    11581 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
    11641 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
    11701 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
    11761 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
    11821 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
    11881 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
    11941 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
    12001 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    12061 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
    12121 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    12181 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    12241 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    12301 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    12361 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    12421 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
    12481 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
    12541 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
    12601 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
    12661 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
    12721 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
    12781 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
    12841 tattacgcca ggggaggcag agattgcagt aagctgagat cgcagcactg cactccagcc
    12901 tgggcgacag agtaagactc tgtctcaaaa ataaaataaa taaatcaatc agatattcca
    12961 atcttttcct ttatttattt atttattttc tattttggaa acacagtcct tccttattcc
    13021 agaattacac atatattcta tttttcttta tatgctccag ttttttttag accttcacct
    13081 gaaatgtgtg tatacaaaat ctaggccagt ccagcagagc ctaaaggtaa aaaataaaat
    13141 aataaaaaat aaataaaatc tagctcactc cttcacatca aaatggagat acagctgtta
    13201 gcattaaata ccaaataacc catcttgtcc tcaataattt taagcgcctc tctccaccac
    13261 atctaactcc tgtcaaaggc atgtgcccct tccgggcgct ctgctgtgct gccaaccaac
    13321 tggcatgtgg actctgcagg gtccctaact gccaagcccc acagtgtgcc ctgaggctgc
    13381 cccttccttc tagcggctgc ccccactcgg ctttgctttc cctagtttca gttacttgcg
    13441 ttcagccaag gtctgaaact aggtgcgcac agagcggtaa gactgcgaga gaaagagacc
    13501 agctttacag ggggtttatc acagtgcacc ctgacagtcg tcagcctcac agggggttta
    13561 tcacattgca ccctgacagt cgtcagcctc acagggggtt tatcacagtg cacccttaca
    13621 atcattccat ttgattcaca atttttttag tctctactgt gcctaacttg taagttaaat
    13681 ttgatcagag gtgtgttccc agaggggaaa acagtatata cagggttcag tactatcgca
    13741 tttcaggcct ccacctgggt cttggaatgt gtcccccgag gggtgatgac tacctcagtt
    13801 ggatctccac aggtcacagt gacacaagat aaccaagaca cctcccaagg ctaccacaat
    13861 gggccgccct ccacgtgcac atggccggag gaactgccat gtcggaggtg caagcacacc
    13921 tgcgcatcag agtccttggt gtggagggag ggaccagcgc agcttccagc catccacctg
    13981 atgaacagaa cctagggaaa gccccagttc tacttacacc aggaaaggc
//
