LOCUS       sequence-194695-        1047 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses OsTIR1 via the ADH1 promoter, and the mGFP-AID via the 
            TEF1 promoter in yeast. Both cassettes are integrated in HO locus. 
            Assembly and characterization of the auxin-inducible degradation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Papagiannakis A, Niebel B, Wit EC, Heinemann M
  TITLE     Autonomous Metabolic Oscillations Robustly Gate the Early and Late 
            Cell Cycle.
  JOURNAL   Mol Cell. 2017 Jan 19;65(2):285-295. doi: 
            10.1016/j.molcel.2016.11.018. Epub 2016 Dec 15.
  PUBMED    27989441
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2016.11.018"; journalName: "Mol Cell"; date: 
            "2017-01-19- 19"; volume: "65"; issue: "2"; pages: "285-295"
COMMENT     Sequence Label: pG2J
FEATURES             Location/Qualifiers
     source          1..1047
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(27..45)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        113..217
                     /gene="bla"
                     /label=AmpR promoter
     CDS             402..780
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(436..455)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
       61 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
      121 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
      181 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
      241 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
      301 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
      361 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
      421 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
      481 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
      541 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
      601 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
      661 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
      721 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
      781 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
      841 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
      901 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
      961 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     1021 ggatgaacga aatagacaga tcgctga
//
