LOCUS       sequence-194741-        9360 bp    DNA     circular SYN 15-APR-2025
DEFINITION  EBNA episome plasmid for U6 promoter-driven expression of a control 
            gRNA targeting TdTomato sequence. Includes PGK-puro selection 
            cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9360)
  AUTHORS   Weltner J, Balboa D, Katayama S, Bespalov M, Krjutskov K, Jouhilahti
            EM, Trokovic R, Kere J, Otonkoski T
  TITLE     Human pluripotent reprogramming with CRISPR activators.
  JOURNAL   Nat Commun. 2018 Jul 6;9(1):2643. doi: 10.1038/s41467-018-05067-x.
  PUBMED    29980666
REFERENCE   2  (bases 1 to 9360)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-07-6"; volume: "9(1):2643. doi"; pages: " 
            10.1038/s41467-018-05067-x"
COMMENT     Sequence Label: GG-EBNA-TdT-guide1-PGK-Puro
FEATURES             Location/Qualifiers
     source          1..9360
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(190..209)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     309..331
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(369..387)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        455..559
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(560..628,629..1420)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(778..797)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1591..2179
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2080..2099
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    2467..2488
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2503..2508,2509..2526,2527..2533)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2541..2557
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2546..2568
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2565..2581
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2600..2619
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2600..2618
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2684..2701
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2685..2701
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2702..2950
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2702..2722
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2873..2892
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        2966..3041
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    3199..3232
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        3293..3790
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3328..3351)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3723..3742
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3830..4429
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3830..3849)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4326..4346
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4878..6803
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     protein_bind    6971..7004
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      7191..8980
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 gcactgcccc gcgaagaagg ggcagagatg tcgtagtcag gtttagttcg tccggggcgg
       61 ggcatcgatc ctctagtaag ggattttgcc gatttcggcc tattggttaa aaaatgagct
      121 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg
      181 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
      241 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
      301 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
      361 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
      421 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
      481 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
      541 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
      601 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
      661 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
      721 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
      781 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
      841 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
      901 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
      961 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     1021 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     1081 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     1141 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     1201 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     1261 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     1321 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     1381 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     1441 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     1501 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     1561 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     1621 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     1681 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     1741 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     1801 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     1861 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     1921 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     1981 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     2041 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     2101 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     2161 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     2221 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     2281 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     2341 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     2401 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     2461 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     2521 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     2581 ccatgattac gccaagctct aatacgactc actataggga gacaagcttg catgcctgca
     2641 ggtcgactct agaggatctc gagctcaagc ttcgaattcc ccctgtaaaa cgacggccag
     2701 tgagggccta tttcccatga ttccttcata tttgcatata cgatacaagg ctgttagaga
     2761 gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata cgtgacgtag
     2821 aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa tggactatca
     2881 tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg
     2941 acgaaacacc ggagttcgag atcgagtttt agagctagaa atagcaagtt aaaataaggc
     3001 tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg ctttttttca tggtcatagc
     3061 tgtttcctca tgagcggccg caggaacccc tagtgatgga gttggccact ccctctctgc
     3121 gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgccca attccgatca
     3181 tattcaataa cccttaatat aacttcgtat aatgtatgct atacgaagtt attaggtctg
     3241 aagaggagtt tacgtccagc caagcttagg atctcgacct cgaaattcta ccgggtaggg
     3301 gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct ggcacttggc
     3361 gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg
     3421 ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc
     3481 cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta
     3541 gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg
     3601 gccaatagca gctttgctcc ttcgctttct gggctcagca gctgggaagg gtgggtccgg
     3661 gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc
     3721 ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc
     3781 gggcctttcg acctgcatcc atctagatct cgagcagctg aagcttacca tgaccgagta
     3841 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     3901 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     3961 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     4021 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
     4081 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     4141 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     4201 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     4261 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     4321 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     4381 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca
     4441 cgacccgcag cgcccgaccg aaaggagcgc acgaccccat gcatcgataa gctgatcctc
     4501 acaggccgca cccagctttt cttccgttgc cccagtagca tctctgtctg gtgaccttga
     4561 agaggaagag gaggggtccc gagaatcccc atccctaccg tccagcaaaa agggggacga
     4621 ggaatttgag gcctggcttg aggctcagga cgcaaatctt gaggatgttc agcgggagtt
     4681 ttccgggctg cgagtaattg gtgatgagga cgaggatggt tcggaggatg gggaattttc
     4741 agacctggat ctgtctgaca gcgaccatga aggggatgag ggtggggggg ctgttggagg
     4801 gggcaggagt ctgcactccc tgtattcact gagcgtcgtc taataaagat gtctattgat
     4861 ctcttttagt gtgaatcatg tctgacgagg ggccaggtac aggacctgga aatggcctag
     4921 gagagaaggg agacacatct ggaccagaag gctccggcgg cagtggacct caaagaagag
     4981 ggggtgataa ccatggacga ggacggggaa gaggacgagg acgaggaggc ggaagaccag
     5041 gagccccggg cggctcagga tcagggccaa gacatagaga tggtgtccgg agaccccaaa
     5101 aacgtccaag ttgcattggc tgcaaaggga cccacggtgg aacaggagca ggagcaggag
     5161 cgggaggggc aggagcagga ggggcaggag caggaggagg ggcaggagca ggaggagggg
     5221 caggaggggc aggaggggca ggaggggcag gagcaggagg aggggcagga gcaggaggag
     5281 gggcaggagg ggcaggaggg gcaggagcag gaggaggggc aggagcagga ggaggggcag
     5341 gaggggcagg agcaggagga ggggcaggag gggcaggagg ggcaggagca ggaggagggg
     5401 caggagcagg aggaggggca ggaggggcag gagcaggagg aggggcagga ggggcaggag
     5461 gggcaggagc aggaggaggg gcaggagcag gaggggcagg aggggcagga ggggcaggag
     5521 caggaggggc aggagcagga ggaggggcag gaggggcagg aggggcagga gcaggagggg
     5581 caggagcagg aggggcagga gcaggagggg caggagcagg aggggcagga ggggcaggag
     5641 caggaggggc aggaggggca ggagcaggag gggcaggagg ggcaggagca ggaggagggg
     5701 caggaggggc aggagcagga ggaggggcag gaggggcagg agcaggaggg gcaggagggg
     5761 caggagcagg aggggcagga ggggcaggag caggaggggc aggaggggca ggagcaggag
     5821 gaggggcagg agcaggaggg gcaggagcag gaggtggagg ccggggtcga ggaggcagtg
     5881 gaggccgggg tcgaggaggt agtggaggcc ggggtcgagg aggtagtgga ggccgccggg
     5941 gtagaggacg tgaaagagcc agggggggaa gtcgtgaaag agccaggggg agaggtcgtg
     6001 gacgtggaga aaagaggccc aggagtccca gtagtcagtc atcatcatcc gggtctccac
     6061 cgcgcaggcc ccctccaggt agaaggccat ttttccaccc tgtaggggaa gccgattatt
     6121 ttgaatacca ccaagaaggt ggcccagatg gtgagcctga cgtgcccccg ggagcgatag
     6181 agcagggccc cgcagatgac ccaggagaag gcccaagcac tggaccccgg ggtcagggtg
     6241 atggaggcag gcgcaaaaaa ggagggtggt ttggaaagca tcgtggtcaa ggaggttcca
     6301 acccgaaatt tgagaacatt gcagaaggtt taagagctct cctggctagg agtcacgtag
     6361 aaaggactac cgacgaagga acttgggtcg ccggtgtgtt cgtatatgga ggtagtaaga
     6421 cctcccttta caacctaagg cgaggaactg cccttgctat tccacaatgt cgtcttacac
     6481 cattgagtcg tctccccttt ggaatggccc ctggacccgg cccacaacct ggcccgctaa
     6541 gggagtccat tgtctgttat ttcatggtct ttttacaaac tcatatattt gctgaggttt
     6601 tgaaggatgc gattaaggac cttgttatga caaagcccgc tcctacctgc aatatcaggg
     6661 tgactgtgtg cagctttgac gatggagtag atttgcctcc ctggtttcca cctatggtgg
     6721 aaggggctgc cgcggagggt gatgacggag atgacggaga tgaaggaggt gatggagatg
     6781 agggtgagga agggcaggag tgatgtaact tgttaggaga cgccctcaat cgtattaaaa
     6841 gccgtgtatt cccccgcact aaagaataaa tccccagtag acatcatgcg tgctgttggt
     6901 gtatttctgg ccatctgtct tgtcaccatt ttcgtcctcc caacatgggg caattgccgg
     6961 aacccttaat ataacttcgt ataatgtatg ctatacgaag ttattaggtc cctcgaagag
     7021 gttcactagc ggatctcaat tgggcatacc catgttgtca cgtcactcag ctccgcgctc
     7081 aacaccttct cgcgttggaa aacattagcg acatttacct ggtgagcaat cagacatgcg
     7141 acggctttag cctggcctcc ttaaattcac ctaagaatgg gagcaaccag caggaaaagg
     7201 acaagcagcg aaaattcacg cccccttggg aggtggcggc atatgcaaag gatagcactc
     7261 ccactctact actgggtatc atatgctgac tgtatatgca tgaggatagc atatgctacc
     7321 cggatacaga ttaggatagc atatactacc cagatataga ttaggatagc atatgctacc
     7381 cagatataga ttaggatagc ctatgctacc cagatataaa ttaggatagc atatactacc
     7441 cagatataga ttaggatagc atatgctacc cagatataga ttaggatagc ctatgctacc
     7501 cagatataga ttaggatagc atatgctacc cagatataga ttaggatagc atatgctatc
     7561 cagatatttg ggtagtatat gctacccaga tataaattag gatagcatat actaccctaa
     7621 tctctattag gatagcatat gctacccgga tacagattag gatagcatat actacccaga
     7681 tatagattag gatagcatat gctacccaga tatagattag gatagcctat gctacccaga
     7741 tataaattag gatagcatat actacccaga tatagattag gatagcatat gctacccaga
     7801 tatagattag gatagcctat gctacccaga tatagattag gatagcatat gctatccaga
     7861 tatttgggta gtatatgcta cccatggcaa cattagccca ccgtgctctc agcgacctcg
     7921 tgaatatgag gaccaacaac cctgtgcttg gcgctcaggc gcaagtgtgt gtaatttgtc
     7981 ctccagatcg cagcaatcgc gcccctatct tggcccgccc acctacttat gcaggtattc
     8041 cccggggtgc cattagtggt tttgtgggca agtggtttga ccgcagtggt tagcggggtt
     8101 acaatcagcc aagttattac acccttattt tacagtccaa aaccgcaggg cggcgtgtgg
     8161 gggctgacgc gtgcccccac tccacaattt caaaaaaaag agtggccact tgtctttgtt
     8221 tatgggcccc attggcgtgg agccccgttt aattttcggg ggtgttagag acaaccagtg
     8281 gagtccgctg ctgtcggcgt ccactctctt tccccttgtt acaaatagag tgtaacaaca
     8341 tggttcacct gtcttggtcc ctgcctggga cacatcttaa taaccccagt atcatattgc
     8401 actaggatta tgtgttgccc atagccataa attcgtgtga gatggacatc cagtctttac
     8461 ggcttgtccc caccccatgg atttctattg ttaaagatat tcagaatgtt tcattcctac
     8521 actagtattt attgcccaag gggtttgtga gggttatatt ggtgtcatag cacaatgcca
     8581 ccactgaacc ccccgtccaa attttattct gggggcgtca cctgaaacct tgttttcgag
     8641 cacctcacat acaccttact gttcacaact cagcagttat tctattagct aaacgaagga
     8701 gaatgaagaa gcaggcgaag attcaggaga gttcactgcc cgctccttga tcttcagcca
     8761 ctgcccttgt gactaaaatg gttcactacc ctcgtggaat cctgacccca tgtaaataaa
     8821 accgtgacag ctcatggggt gggagatatc gctgttcctt aggacccttt tactaaccct
     8881 aattcgatag catatgcttc ccgttgggta acatatgcta ttgaattagg gttagtctgg
     8941 atagtatata ctactacccg ggaagcatat gctacccgtt tagggttaac aagggggcct
     9001 tataaacact attgctaatg ccctcttgag ggtccgctta tcggtagcta cacaggcccc
     9061 tctgattgac gttggtgtag cctcccgtag tcttcctggg cccctgggag gtacatgtcc
     9121 cccagcattg gtgtaagagc ttcagccaag agttacacat aaaggcaatg ttgtgttgca
     9181 gtccacagac tgcaaagtct gctccaggat gaaagccact cagtgttggc aaatgtgcac
     9241 atccatttat aaggatgtca actacagtca gagaacccct ttgtgtttgg tccccccccg
     9301 tgtcacatgt ggaacagggc ccagttggca agttgtacca accaactgaa gggattacat
//
