LOCUS       sequence-194756-       10731 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses human DVL1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10731)
  AUTHORS   Susman MW, Karuna EP, Kunz RC, Gujral TS, Cantu AV, Choi SS, Jong 
            BY, Okada K, Scales MK, Hum J, Hu LS, Kirschner MW, Nishinakamura R,
            Yamada S, Laird DJ, Jao LE, Gygi SP, Greenberg ME, Ho HH
  TITLE     Kinesin superfamily protein Kif26b links Wnt5a-Ror signaling to the 
            control of cell and tissue behaviors in vertebrates.
  JOURNAL   Elife. 2017 Sep 8;6. pii: e26509. doi: 10.7554/eLife.26509.
  PUBMED    28885975
REFERENCE   2  (bases 1 to 10731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-09-8"; volume: "6. pii: e26509. doi"; pages: " 
            10.7554/eLife.26509"
COMMENT     Sequence Label: pLEX307 hDVL1
FEATURES             Location/Qualifiers
     source          1..10731
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..1274
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          327..1265
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1222..1242
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1294..1318
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    3416..3550
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(3640..3664)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3672..3713
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3764..4352
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3817..3837)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4235..4246)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4424..4657
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4735..4856
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      4896..5031
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(5052..5070)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5080..5097)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5080..5096)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5089..5111)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5238..5693
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5325..5344)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5535..5556
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5719..5823
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5824..5892,5893..6684)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6042..6061)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6855..7443
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7344..7363
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7597..7614
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7731..7752
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7767..7772,7773..7790,7791..7797)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7805..7821
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7810..7832
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7829..7845
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7829..7845
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7864..7884
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7866..7884
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7912..8138
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8139..8319
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8366..8491
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8984..9217
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9402..9446
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9677..9887
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9699..9895
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9746..9881
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9927..10529
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9927..9946)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10423..10443
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10572..10689
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct
       61 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
      121 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
      181 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      241 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      301 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
      361 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
      421 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
      481 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
      541 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
      601 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
      661 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
      721 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
      781 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
      841 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
      901 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
      961 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     1021 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     1081 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     1141 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     1201 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     1261 ttcaggtgtc gtgaggctag catcgattga tcaacaagtt tgtacaaaaa agcaggctgg
     1321 cgccggaacc aattcagtcg actggatccg gtaccgaatt ccaccatggg cgagaccaag
     1381 attatctacc acatggacga ggaggagacg ccgtacctgg tcaagctgcc cgtggccccc
     1441 gagcgcgtca cgctggccga cttcaagaac gtgctcagca accggcccgt gcacgcctac
     1501 aaattcttct ttaagtccat ggaccaggac ttcggggtgg tgaaggagga gatctttgat
     1561 gacaatgcca agcttccctg cttcaacggc cgcgtggtct cctggctggt cctggctgag
     1621 ggtgctcact cggatgcggg gtcccagggc acggacagcc acacagacct gcccccgcct
     1681 cttgagcgga caggcggcat cggggactcc cggcccccct ccttccaccc gaatgtggcc
     1741 agcagccgtg acgggatgga caacgagaca ggcacggagt ccatggtcag tcaccggcgg
     1801 gagcgtgccc gacgccggaa ccgcgaggag gccgcccgga ccaatgggca cccaagggga
     1861 gaccgacggc gggatgtggg gctgccccca gacagcgcgt ccaccgccct cagcagcgag
     1921 cttgagtcca gcagctttgt ggactcggac gaggatggca gcacgagcag gctcagcagc
     1981 tccacggagc agagcacctc atccagactc atccggaagc acaaacgccg gcggaggaag
     2041 cagcgccttc ggcaggcgga ccgggcctcc tccttcagca gcataaccga ctccaccatg
     2101 tccctcaaca tcgtcactgt cacgctcaac atggaaagac atcactttct gggcatcagc
     2161 atcgtggggc agagcaacga ccgtggagac ggcggcatct acattggctc catcatgaag
     2221 ggcggggctg tggccgctga cggccgcatc gagcccggcg acatgttgct gcaggtgaat
     2281 gacgtgaact ttgagaacat gagcaatgac gatgccgtgc gggtgctgcg ggagatcgtt
     2341 tcccagacgg ggcccatcag cctcactgtg gccaagtgct gggacccaac gccccgaagc
     2401 tacttcaccg tcccacgggc tgacccggtg cggcccatcg accccgccgc ctggctgtcc
     2461 cacacggcgg cactgacagg agccctgccc cgctacgagc tggaagaggc gccgctgacg
     2521 gtgaagagtg acatgagcgc cgtcgtccgg gtcatgcagc tgccagactc gggactggag
     2581 atccgcgacc gcatgtggct caagatcacc atcgccaatg ccgtcatcgg ggcggacgtg
     2641 gtggactggc tgtacacaca cgtggagggc ttcaaggagc ggcgggaggc ccggaagtac
     2701 gccagcagct tgctgaagca cggcttcctg cggcacacgg tcaacaagat caccttctcc
     2761 gagcagtgct actacgtctt cggggatctc tgcagcaatc tcgccaccct gaacctcaac
     2821 agtggctcca gtgggacttc ggatcaggac acgctggccc cgctgcccca cccggctgcc
     2881 ccctggcctc tgggtcaggg ctacccctac cagtacccgg gacccccacc ctgcttcccg
     2941 cctgcctacc aggacccggg ctttagctat ggcagcggca gcaccgggag tcagcagagt
     3001 gaagggagca aaagcagtgg gtccacccgg agcagccgcc gggccccggg ccgtgagaag
     3061 gagcgtcggg cggcgggagc tgggggcagt ggcagtgaat cggatcacac ggcaccgagt
     3121 ggggtgggga gcagctggcg agagcgtccg gccggccagc tcagccgtgg cagcagccca
     3181 cgcagtcagg cctcggctac cgccccgggg ctccccccgc cccaccccac gaccaaggcc
     3241 tatacagtgg tgggggggcc acccggggga ccccctgtcc gggagctggc tgccgtcccc
     3301 ccggaattga caggcagccg ccagtccttc cagaaggcta tggggaaccc ctgcgagttc
     3361 ttcgtggaca tcatgtgagg cgcgcctcta gaactatagt gagtcgtatt acgtagatcc
     3421 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     3481 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     3541 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
     3601 ggaggttttt taattcgcgg ccgcactcga gatatctaga cccagctttc ttgtacaaag
     3661 tggttgatat cggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat
     3721 gaactagtac cggttaagtc gacaatcaac gcgttaagtc gacaatcaac ctctggatta
     3781 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     3841 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     3901 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     3961 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     4021 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     4081 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     4141 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
     4201 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     4261 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     4321 gagtcggatc tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag
     4381 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     4441 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     4501 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     4561 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     4621 ctcagaccct tttagtcagt gtggaaaatc tctagcagta cgtatagtag ttcatgtcat
     4681 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
     4741 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     4801 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     4861 gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     4921 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     4981 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt
     5041 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg
     5101 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     5161 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     5221 cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     5281 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     5341 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     5401 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     5461 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     5521 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     5581 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     5641 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc
     5701 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     5761 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     5821 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     5881 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     5941 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     6001 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     6061 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     6121 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     6181 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     6241 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     6301 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     6361 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     6421 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     6481 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     6541 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     6601 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     6661 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     6721 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     6781 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     6841 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     6901 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     6961 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     7021 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     7081 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     7141 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     7201 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     7261 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     7321 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     7381 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     7441 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     7501 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     7561 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     7621 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     7681 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     7741 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     7801 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     7861 cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt
     7921 atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag
     7981 gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat
     8041 taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca
     8101 gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga
     8161 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     8221 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     8281 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
     8341 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     8401 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     8461 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
     8521 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
     8581 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
     8641 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     8701 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     8761 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     8821 acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
     8881 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
     8941 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
     9001 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
     9061 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
     9121 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     9181 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     9241 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
     9301 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
     9361 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
     9421 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
     9481 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
     9541 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
     9601 tcccaacccc gaggggaccc atgcattgca tctcaattag tcagcaacca ggtgtggaaa
     9661 gtccccaggc tccccagcag gcagaagtat gcaaagcatg cgtctcaatt agtcagcaac
     9721 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     9781 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     9841 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     9901 ttctagaggt accaccggag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca
     9961 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
    10021 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
    10081 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
    10141 ccgtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
    10201 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
    10261 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
    10321 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
    10381 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
    10441 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
    10501 gcatgacccg caagcccggt gccagttaac gaattctaga tcttgagaca aatggcagta
    10561 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
    10621 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
    10681 caaaattttc gggtttatta cagggacagc agagatccac tttggcgccg g
//
