LOCUS       sequence-195305-       11490 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Doxycyline regulated expression of Gata6 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11490)
  AUTHORS   Lalit PA, Salick MR, Nelson DO, Squirrell JM, Shafer CM, Patel NG, 
            Saeed I, Schmuck EG, Markandeya YS, Wong R, Lea MR, Eliceiri KW, 
            Hacker TA, Crone WC, Kyba M, Garry DJ, Stewart R, Thomson JA, Downs 
            KM, Lyons GE, Kamp TJ
  TITLE     Lineage Reprogramming of Fibroblasts into Proliferative Induced 
            Cardiac Progenitor Cells by Defined Factors.
  JOURNAL   Cell Stem Cell. 2016 Mar 3;18(3):354-67. doi: 
            10.1016/j.stem.2015.12.001. Epub 2016 Feb 11.
  PUBMED    26877223
REFERENCE   2  (bases 1 to 11490)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2015.12.001"; journalName: "Cell Stem Cell"; date: 
            "2016-03-3- 3"; volume: "18"; issue: "3"; pages: "354-67"
COMMENT     Sequence Label: pSAM2_mCherry_Gata6
FEATURES             Location/Qualifiers
     source          1..11490
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        13..392
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        394..592
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     547..567
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             610..790
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    837..962
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1459..1692
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1877..1921
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2219..2336
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2405..2423
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2442..2460
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2479..2497
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2516..2534
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2553..2571
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2590..2608
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2627..2645
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2664..2682
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2704..2728
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2801..2825
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3812..3832
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     protein_bind    complement(4651..4675)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4745..5206
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4801..4818)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             5211..5921
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYGKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5356..5374)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5624..5643
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    5954..6542
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6007..6027)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6425..6436)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6545..6561)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             7070..7250
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7276..7293)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7282..7506
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7552..7980
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7639..7658)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7849..7870
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7989..8009)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7994..8323
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8174..8309
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8236..8255
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8371..8418
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8437..8811
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8941..9062
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8978..8997)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9032..9051
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9111..9127)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9111..9127)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9124..9146)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9135..9151
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9159..9165,9166..9183,9184..9189))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9204..9225
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9342..9359)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9513..10101)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9593..9612)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10272..11063,11064..11132))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10895..10914
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11133..11237)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11312..11331)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
       61 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
      121 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
      181 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
      241 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
      301 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
      361 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
      421 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
      481 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
      541 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agcgcgtttt
      601 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
      661 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
      721 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
      781 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
      841 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
      901 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
      961 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
     1021 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
     1081 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
     1141 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
     1201 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
     1261 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag
     1321 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     1381 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     1441 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     1501 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     1561 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     1621 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     1681 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     1741 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     1801 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     1861 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     1921 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     1981 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     2041 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
     2101 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
     2161 gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt
     2221 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     2281 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
     2341 tttattacag ggacagcaga gatccagttt ggttacgcgt ccccgaaaag tgccacctga
     2401 cgtctcccta tcagtgatag agaagtcgac acgtctcgag ctccctatca gtgatagaga
     2461 aggtacgtct agaacgtctc cctatcagtg atagagaagt cgacacgtct cgagctccct
     2521 atcagtgata gagaaggtac gtctagaacg tctccctatc agtgatagag aagtcgacac
     2581 gtctcgagct ccctatcagt gatagagaag gtacgtctag aacgtctccc tatcagtgat
     2641 agagaagtcg acacgtctcg agctccctat cagtgataga gaaggtaccc cctatataag
     2701 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     2761 ccatagaaga caccgggacc gatccagcct gctagcatca acaagtttgt acaaaaaagc
     2821 aggctccgaa ttcgcccttg gacagtggat ggccttgact gacggcggct ggtgcctgcc
     2881 gaagcgtttc ggggctgctg ctgcggacgc cggcgactcc gggccctttc cagcgcggga
     2941 gccctcctcg ccgctttccc ccatctcgtc ttcgtcctcc tcctgctccc ggggcgggga
     3001 tcgcggtccc tgcggcgcca gcaactgcag gacgccgcag ctcgacgccg aggcggtggc
     3061 gggacctccg ggccgctcgc tcttgctcag cccctacgcc tcgcatccct tcgccgctgc
     3121 ccacggagcc gcggcgcccg gggtcgcagg ccccgggagc gccctgtcga cttgggagga
     3181 cctgttgctc ttcactgacc tcgatcaggc cgcgaccgcc agcaagctgt tgtggtccag
     3241 ccggggcgcc aaactgagcc ccttcgcggc cgagcagccg gaggaaatgt accagaccct
     3301 cgccgccctg tccagccagg ggcccgccgc ttacgacggc gcgcccggcg gcttcgtgca
     3361 ctccgcagcg gcggcggccg ctgccgccgc ggcagccagc tcccctgtct acgtgcccac
     3421 cacgcgcgtg ggctccatgc tgtccggcct gccctacctt caaggcgcgg gcagcgggcc
     3481 cagcaatcac gcgggcggag cgggtgccca cccaggctgg tcccaggcct ccgccgacag
     3541 ccccccgtat ggcgggggtg gcgcagccgg cggcggcgcg gccggacctg gaggtgctgg
     3601 atcggctacg gcccacgcct ctgcacggtt tccctactcg cccagcccgc ccatggccaa
     3661 cggcgccgcg cgagaccccg ggggctacgt ggctgcgggc ggcacgggcg caggcagtgt
     3721 gagtggaggt ggcggcagcc tggcggccat gggtggccgg gagcaccagt acagctcgct
     3781 gtccgcagct cggccgctga acggaacgta ccaccaccac catcaccatc acccgaccta
     3841 ctcgccctac atggccgcac cgctgactcc tgcctggcca gcaggaccct tcgaaacgcc
     3901 ggtgctccac agcttacagg gccgcgcggg agctccactc ccggtgccac gaggccccag
     3961 cacagacctg ttggaggacc tgtcggagag ccgcgagtgc gtgaactgcg gctccatcca
     4021 gacgccactg tggagacgag acggcaccgg tcattacctg tgcaatgcat gcggtctcta
     4081 cagcaagatg aatggcctca gcaggcccct catcaagcca cagaagcgcg tgccttcatc
     4141 acggcggctt ggactgtcct gtgccaactg tcacaccaca accactacct tatggcgtag
     4201 aaatgctgag ggtgagcctg tgtgcaatgc ttgcgggctc tatatgaaac tccatggggt
     4261 gcctcgacca cttgctatga aaaaagaagg aattcaaacc aggaaacgaa aacctaaaaa
     4321 tataaataag tcaaaagctt gctccggtaa cagcagtggc tctgtcccta tgactcctac
     4381 ttcctcttct tctaattcag atgactgcac caaaaatact tctccttcta cacaagcgac
     4441 cacctcaggg gtaggggcat cagtgatgtc tgcagtggga gaaaacgcca accccgagaa
     4501 cagtgacctc aagtattcag gtcaagacgg cctctacata ggtgtcagtc tgtcctcccc
     4561 tgccgaagtc acatcctccg tgcgacagga ttcttggtgt gctctggccc tggcctgagc
     4621 tggtgctacc aagaggcaag ggcgaattcg acccagcttt cttgtacaaa gtggttgatg
     4681 cggccgcttc gagcagacat gagtcgacgt tattttccac catattgccg tcttttggca
     4741 atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc
     4801 ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag
     4861 cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg
     4921 gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac
     4981 aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctcacctcaa
     5041 gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc
     5101 tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaac gtctaggccc
     5161 cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataatacc atggtgagca
     5221 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
     5281 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     5341 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
     5401 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgggaagcac cccgccgaca
     5461 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     5521 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
     5581 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
     5641 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
     5701 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
     5761 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt
     5821 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
     5881 gccgccactc caccggcggc atggacgagc tgtacaagta agtcgaccat aattcgatat
     5941 caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     6001 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     6061 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     6121 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     6181 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     6241 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     6301 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     6361 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     6421 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     6481 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     6541 gcatcgatac cgtcgacctc gtcgagacct agaaaaacat ggagcaatca caagtagcaa
     6601 tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg
     6661 ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct
     6721 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     6781 agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt ggcagaacta
     6841 cacaccaggg ccagggatca gatatccact gacctttgga tggtgctaca agctagtacc
     6901 agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct tgttacaccc
     6961 tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga ggtttgacag
     7021 ccgcctagca tttcatcaca tggcccgaga gctgcatccg gactgtactg ggtctctctg
     7081 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     7141 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     7201 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gggcccgttt
     7261 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     7321 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     7381 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     7441 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     7501 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
     7561 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     7621 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     7681 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     7741 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     7801 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     7861 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     7921 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     7981 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     8041 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     8101 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     8161 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     8221 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
     8281 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
     8341 tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc
     8401 ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt
     8461 ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg
     8521 ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg
     8581 ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg
     8641 cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac
     8701 gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg
     8761 cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg acacgtgcta
     8821 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     8881 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
     8941 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     9001 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     9061 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     9121 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     9181 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     9241 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     9301 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     9361 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     9421 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     9481 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     9541 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     9601 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     9661 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     9721 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     9781 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     9841 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     9901 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     9961 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
    10021 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
    10081 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
    10141 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
    10201 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
    10261 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
    10321 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
    10381 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
    10441 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
    10501 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
    10561 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
    10621 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
    10681 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
    10741 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
    10801 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    10861 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
    10921 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    10981 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    11041 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    11101 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    11161 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    11221 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga
    11281 gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
    11341 gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt
    11401 aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc
    11461 gttttgcgct gcttcgcgat gtacgggcca
//
