LOCUS       sequence-19625-e         739 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Loayza D, De Lange T
  TITLE     POT1 as a terminal transducer of TRF1 telomere length control.
  JOURNAL   Nature. 2003 Jun 26. 423(6943):1013-8.
  PUBMED    12768206
REFERENCE   2  (bases 1 to 739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2003 Jun 26. 423(6943):1013-8."
COMMENT     Sequence Label: pLPC myc hPOT1
FEATURES             Location/Qualifiers
     source          1..739
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..51
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        32..50
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      82..91
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             91..120
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 agaacccact ggcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag
       61 cttggtaccg agctcggatc agccaccatg gaacaaaagt tgatttctga agaagatttg
      121 ttaagaaaga gaggatcctt ggttccagca acaaattata tatatacacc cctgaatcaa
      181 cttaagggtg gtacaattgt caatgtctat ggtgttgtga agttctttaa gcccccatat
      241 ctaagcaaag gaactgatta ttgctcagtt gtaactattg tggaccagac aaatgtaaaa
      301 ctaacttgcc tgctctttag tggaaactat gaagcccttc caataattta taaaaatgga
      361 gatattgttc gctttcacag gctgaagatt caagtatata aaaaggagac tcagggtatc
      421 accagctctg gctttgcatc tttgacgttt gagggaactt tgggagcccc tatcatacct
      481 cgcacttcaa gcaagtattt taacttcact actgaggacc acaaaatggt agaagcctta
      541 cgtgtttggg catctactca tatgtcaccg tcttggacat tactaaaatt gtgtgatgtt
      601 cagccaatgc agtattttga cctgacttgt cagctcttgg gcaaagcaga agtggacgga
      661 gcatcatttc ttctaaaggt atgggatggc accaggacac catttccatc ttggagagtc
      721 ttaatacaag accttgttc
//
