LOCUS       sequence-197082-        5527 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Luciferase validation vector with GTSE1 enhancer inserted downstream
            of the reporter gene. The candidate's enhancer activity is reflected
            by luciferase activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5527)
  AUTHORS   Muerdter F, Boryn LM, Woodfin AR, Neumayr C, Rath M, Zabidi MA, 
            Pagani M, Haberle V, Kazmar T, Catarino RR, Schernhuber K, Arnold 
            CD, Stark A
  TITLE     Resolving systematic errors in widely used enhancer activity assays 
            in human cells.
  JOURNAL   Nat Methods. 2017 Dec 11. pii: nmeth.4534. doi: 10.1038/nmeth.4534.
  PUBMED    29256496
REFERENCE   2  (bases 1 to 5527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2017 Dec 11. pii: nmeth.4534. doi: 10.1038/nmeth.4534."
COMMENT     Sequence Label: STARR-seq luciferase validation vector_SCP1_GTSE1
FEATURES             Location/Qualifiers
     source          1..5527
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      154..163
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             160..1812
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    1856..1977
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1867..1886)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1921..1940
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(3621..4209)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3701..3720)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4409..5200,5201..5269))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    5374..5422
                     /note="synthetic polyadenylation signal"
     misc_feature    5436..5527
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5476..5495
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggcctaactg gcttagagat ctccctaggg tacttatata agggggtggg ggcgcgttcg
       61 tcctcagtcg cgatcgaaca ctcgagccga gcagacgtgc ctacggaccc gggctagcga
      121 cctaaagctt ggcaatccgg tactgttggt aaagccacca tggaagatgc caaaaacatt
      181 aagaagggcc cagcgccatt ctacccactc gaagacggga ccgccggcga gcagctgcac
      241 aaagccatga agcgctacgc cctggtgccc ggcaccatcg cctttaccga cgcacatatc
      301 gaggtggaca ttacctacgc cgagtacttc gagatgagcg ttcggctggc agaagctatg
      361 aagcgctatg ggctgaatac aaaccatcgg atcgtggtgt gcagcgagaa tagcttgcag
      421 ttcttcatgc ccgtgttggg tgccctgttc atcggtgtgg ctgtggcccc agctaacgac
      481 atctacaacg agcgcgagct gctgaacagc atgggcatca gccagcccac cgtcgtattc
      541 gtgagcaaga aagggctgca aaagatcctc aacgtgcaaa agaagctacc gatcatacaa
      601 aagatcatca tcatggatag caagaccgac taccagggct tccaaagcat gtacaccttc
      661 gtgacttccc atttgccacc cggcttcaac gagtacgact tcgtgcccga gagcttcgac
      721 cgggacaaaa ccatcgccct gatcatgaac agtagtggca gtaccggatt gcccaagggc
      781 gtagccctac cgcaccgcac cgcttgtgtc cgattcagtc atgcccgcga ccccatcttc
      841 ggcaaccaga tcatccccga caccgctatc ctcagcgtgg tgccatttca ccacggcttc
      901 ggcatgttca ccacgctggg ctacttgatc tgcggctttc gggtcgtgct catgtaccgc
      961 ttcgaggagg agctattctt gcgcagcttg caagactata agattcaatc tgccctgctg
     1021 gtgcccacac tatttagctt cttcgctaag agcactctca tcgacaagta cgacctaagc
     1081 aacttgcacg agatcgccag cggcggggcg ccgctcagca aggaggtagg tgaggccgtg
     1141 gccaaacgct tccacctacc aggcatccgc cagggctacg gcctgacaga aacaaccagc
     1201 gccattctga tcacccccga aggggacgac aagcctggcg cagtaggcaa ggtggtgccc
     1261 ttcttcgagg ctaaggtggt ggacttggac accggtaaga cactgggtgt gaaccagcgc
     1321 ggcgagctgt gcgtccgtgg ccccatgatc atgagcggct acgttaacaa ccccgaggct
     1381 acaaacgctc tcatcgacaa ggacggctgg ctgcacagcg gcgacatcgc ctactgggac
     1441 gaggacgagc acttcttcat cgtggaccgg ctgaagagcc tgatcaaata caagggctac
     1501 caggtagccc cagccgaact ggagagcatc ctgctgcaac accccaacat cttcgacgcc
     1561 ggggtcgccg gcctgcccga cgacgatgcc ggcgagctgc ccgccgcagt cgtcgtgctg
     1621 gaacacggta aaaccatgac cgagaaggag atcgtggact atgtggccag ccaggttaca
     1681 accgccaaga agctgcgcgg tggtgttgtg ttcgtggacg aggtgcctaa aggactgacc
     1741 ggcaagttgg acgcccgcaa gatccgcgag attctcatta aggccaagaa gggcggcaag
     1801 atcgccgtgt aataattcta gagtcggggc ggccggccgc ttcgagcaga catgataaga
     1861 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     1921 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     1981 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
     2041 agcaagtaaa acctctacaa atgtggtaaa atcgataagg gagctgggat tcaaagccaa
     2101 gtcaggccgg acacggtggc tcacgcctat aatcccagca ctttgggagg cagaggcggg
     2161 cggatcacga ggccaggaga tcgagaccat cctggctaac acggtgaaac cccatctcta
     2221 ctaaaaaaaa tacaaaaata attagccggg tgtggtggcg ggtgcctgta gtcccagcta
     2281 ctagggaggc tgaggcagga gaatggcgtg aacccgggag gtggagtttg cagtgagccg
     2341 agatcgtgcc actgcactcc agcctgggcg acagagcaag actccatctc aaaaagaaaa
     2401 aaaaagccaa gtcagaatca aagtgccacg ccagtgctct tcaactgcgg tacaagtcac
     2461 agtcacccag gagctttagg aaatccagct gctgggcctc agcctagatg aaccaaatca
     2521 gaacccctgg ggtgggctgg gcacgggcag tcctaagagc gctggaggga cttctgctgt
     2581 gcagcctggc atggcaacca caccagtcca gggctgggag ctgccgtact gcatcgcgga
     2641 agtctcagat gtcttaagtc tgcagtagat ctaaaagggg ctggtacaca tcaggagctc
     2701 ggcctgcacc caagagactg gcgtggaagc cagatgcgca ggccaggctg cagggttacc
     2761 aagtggttga ggtaagcaga tgcgtgagtc attctacagc aataaagaga ggaagcggac
     2821 caacgtcact cacctgccaa ctgacgtgca cgactgcggc cgctgtgccc gcgaaagctt
     2881 tggtgttgag ctgtccgggg agtcacctaa gtccaagaaa ggaagtgtgc ggtcaggtaa
     2941 agtgatcagg gggagaccta ctgttggggg atcgtcaccg aatgggcaat tctccgagta
     3001 tctccactgt cccaacttga gtgtaggtgg cactggctca gacaagtaca caaagggcca
     3061 ccccgctgca ggcagccaca tgagaggcat tcacccctac acggggaccc ctgcccaggc
     3121 aaaggctgtg gtctgatgga tggacacggg ggagaaagag aagagccatc ttcactgcgc
     3181 cgccccaccc tggctgtata tcccatcagg gccgagcacg accttcacag agcgaagtcg
     3241 acttgtcaga cggcacctgc atgcgctaga cacttgccca caggaacaac accgcaggga
     3301 tttagcttcc caggcacgcg caacagagcc cacattccct cattttcccg aaccatgact
     3361 ttactgggat aataataatt cattacactg tgtttgtgct gttttagttg acaagcattt
     3421 ccatgtgtat gatctcatct gatattcaca acaggatgaa aagcaggctc agagggcaaa
     3481 ggggtcacca gttaccgagc tagggcccaa cacaggcccc agaggtccag tcccgccacc
     3541 acgcccccag acactggctc catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     3601 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     3661 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     3721 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     3781 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     3841 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     3901 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     3961 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     4021 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     4081 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     4141 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     4201 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     4261 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     4321 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagc
     4381 ggccgcaaat gctaaaccac tgcagtggtt accagtgctt gatcagtgag gcaccgatct
     4441 cagcgatctg cctatttcgt tcgtccatag tggcctgact ccccgtcgtg tagatcacta
     4501 cgattcgtga gggcttacca tcaggcccca gcgcagcaat gatgccgcga gagccgcgtt
     4561 caccggcccc cgatttgtca gcaatgaacc agccagcagg gagggccgag cgaagaagtg
     4621 gtcctgctac tttgtccgcc tccatccagt ctatgagctg ctgtcgtgat gctagagtaa
     4681 gaagttcgcc agtgagtagt ttccgaagag ttgtggccat tgctactggc atcgtggtat
     4741 cacgctcgtc gttcggtatg gcttcgttca actctggttc ccagcggtca agccgggtca
     4801 catgatcacc catattatga agaaatgcag tcagctcctt agggcctccg atcgttgtca
     4861 gaagtaagtt ggccgcggtg ttgtcgctca tggtaatggc agcactacac aattctctta
     4921 ccgtcatgcc atccgtaaga tgcttttccg tgaccggcga gtactcaacc aagtcgtttt
     4981 gtgagtagtg tatacggcga ccaagctgct cttgcccggc gtctatacgg gacaacaccg
     5041 cgccacatag cagtactttg aaagtgctca tcatcgggaa tcgttcttcg gggcggaaag
     5101 actcaaggat cttgccgcta ttgagatcca gttcgatata gcccactctt gcacccagtt
     5161 gatcttcagc atcttttact ttcaccagcg tttcggggtg tgcaaaaaca ggcaagcaaa
     5221 atgccgcaaa gaagggaatg agtgcgacac gaaaatgttg gatgctcata ctcgtccttt
     5281 ttcaatatta ttgaagcatt tatcagggtt actagtacgt ctctcaagga taagtaagta
     5341 atattaaggt acgggaggta ttggacaggc cgcaataaaa tatctttatt ttcattacat
     5401 ctgtgtgttg gttttttgtg tgaatcgata gtactaacat acgctctcca tcaaaacaaa
     5461 acgaaacaaa acaaactagc aaaataggct gtccccagtg caagtgcagg tgccagaaca
     5521 tttctct
//
