LOCUS       sequence-197532-       10411 bp    DNA     circular SYN 15-APR-2025
DEFINITION  fluorescent tagging. Plant expression of RFP-35S:RabG3B-CA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10411)
  TITLE     Robatzek Lab plasmids
REFERENCE   2  (bases 1 to 10411)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: UBI10:RFP-RabG3B-CA
FEATURES             Location/Qualifiers
     source          1..10411
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      33..621
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     522..541
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     775..792
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    807..947
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(873..892)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1033..1055
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      1291..1485
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(1551..2624)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(3053..3682)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    5013..5037
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    5202..5223
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5238..5243,5244..5261,5262..5268)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5276..5292
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5281..5303
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5300..5316
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5300..5316
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             6004..6678
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             6004..6678
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     6402..6421
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     6596..6619
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     protein_bind    6691..6715
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7331..7355)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        7725..7904
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             7946..8497
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      8517..8769
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    8862..8886
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             9407..10198
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
       61 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
      121 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
      181 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
      241 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
      301 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
      361 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
      421 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
      481 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
      541 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
      601 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
      661 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
      721 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
      781 tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc
      841 ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
      901 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc
      961 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     1021 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     1081 cgaggcaggg tgccttgatg tgggcgccgg cggtcgagtg gcgacggcgc ggcttgtccg
     1141 cgccctggta gattgcctgg ccctaggcca gccatttttg agcggccagc ggccgcgata
     1201 ggccgacgcg aagcggcggg gcgtagggag cgcagcgacc gaagggtagg cgctttttgc
     1261 agctcttcgg ctgtgcgctg gccagacagt tatgcacagg ccaggcgggt tttaagagtt
     1321 ttaataagtt ttaaagagtt ttaggcggaa aaatcgcctt ttttctcttt tatatcagtc
     1381 acttacatgt gtgaccggtt cccaatgtac ggctttgggt tcccaatgta cgggttccgg
     1441 ttcccaatgt acggctttgg gttcccaatg tacgtgctat ccacaggaaa gagacctttt
     1501 cgaccttttt cccctgctag ggcaatttgc cctagcatct gctccgtaca ttaggaaccg
     1561 gcggatgctt cgccctcgat caggttgcgg tagcgcatga ctaggatcgg gccagcctgc
     1621 cccgcctcct ccttcaaatc gtactccggc aggtcatttg acccgatcag cttgcgcacg
     1681 gtgaaacaga acttcttgaa ctctccggcg ctgccactgc gttcgtagat cgtcttgaac
     1741 aaccatctgg cttctgcctt gcctgcggcg cggcgtgcca ggcggtagag aaaacggccg
     1801 atgccgggat cgatcaaaaa gtaatcgggg tgaaccgtca gcacgtccgg gttcttgcct
     1861 tctgtgatct cgcggtacat ccaatcagct agctcgatct cgatgtactc cggccgcccg
     1921 gtttcgctct ttacgatctt gtagcggcta atcaaggctt caccctcgga taccgtcacc
     1981 aggcggccgt tcttggcctt cttcgtacgc tgcatggcaa cgtgcgtggt gtttaaccga
     2041 atgcaggttt ctaccaggtc gtctttctgc tttccgccat cggctcgccg gcagaacttg
     2101 agtacgtccg caacgtgtgg acggaacacg cggccgggct tgtctccctt cccttcccgg
     2161 tatcggttca tggattcggt tagatgggaa accgccatca gtaccaggtc gtaatcccac
     2221 acactggcca tgccggccgg ccctgcggaa acctctacgt gcccgtctgg aagctcgtag
     2281 cggatcacct cgccagctcg tcggtcacgc ttcgacagac ggaaaacggc cacgtccatg
     2341 atgctgcgac tatcgcgggt gcccacgtca tagagcatcg gaacgaaaaa atctggttgc
     2401 tcgtcgccct tgggcggctt cctaatcgac ggcgcaccgg ctgccggcgg ttgccgggat
     2461 tctttgcgga ttcgatcagc ggccgcttgc cacgattcac cggggcgtgc ttctgcctcg
     2521 atgcgttgcc gctgggcggc ctgcgcggcc ttcaacttct ccaccaggtc atcacccagc
     2581 gccgcgccga tttgtaccgg gccggatggt ttgcgaccgc tcacgccgat tcctcgggct
     2641 tgggggttcc agtgccattg cagggccggc agacaaccca gccgcttacg cctggccaac
     2701 cgcccgttcc tccacacatg gggcattcca cggcgtcggt gcctggttgt tcttgatttt
     2761 ccatgccgcc tcctttagcc gctaaaattc atctactcat ttattcattt gctcatttac
     2821 tctggtagct gcgcgatgta ttcagatagc agctcggtaa tggtcttgcc ttggcgtacc
     2881 gcgtacatct tcagcttggt gtgatcctcc gccggcaact gaaagttgac ccgcttcatg
     2941 gctggcgtgt ctgccaggct ggccaacgtt gcagccttgc tgctgcgtgc gctcggacgg
     3001 ccggcactta gcgtgtttgt gcttttgctc attttctctt tacctcatta actcaaatga
     3061 gttttgattt aatttcagcg gccagcgcct ggacctcgcg ggcagcgtcg ccctcgggtt
     3121 ctgattcaag aacggttgtg ccggcggcgg cagtgcctgg gtagctcacg cgctgcgtga
     3181 tacgggactc aagaatgggc agctcgtacc cggccagcgc ctcggcaacc tcaccgccga
     3241 tgcgcgtgcc tttgatcgcc cgcgacacga caaaggccgc ttgtagcctt ccatccgtga
     3301 cctcaatgcg ctgcttaacc agctccacca ggtcggcggt ggcccatatg tcgtaagggc
     3361 ttggctgcac cggaatcagc acgaagtcgg ctgccttgat cgcggacaca gccaagtccg
     3421 ccgcctgggg cgctccgtcg atcactacga agtcgcgccg gccgatggcc ttcacgtcgc
     3481 ggtcaatcgt cgggcggtcg atgccgacaa cggttagcgg ttgatcttcc cgcacggccg
     3541 cccaatcgcg ggcactgccc tggggatcgg aatcgactaa cagaacatcg gccccggcga
     3601 gttgcagggc gcgggctaga tgggttgcga tggtcgtctt gcctgacccg cctttctggt
     3661 taagtacagc gataaccttc atgcgttccc cttgcgtatt tgtttattta ctcatcgcat
     3721 catatacgca gcgaccgcat gacgcaagct gttttactca aatacacatc acctttttag
     3781 acggcggcgc tcggtttctt cagcggccaa gctggccggc caggccgcca gcttggcatc
     3841 agacaaaccg gccaggattt catgcagccg cacggttgag acgtgcgcgg gcggctcgaa
     3901 cacgtacccg gccgcgatca tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc
     3961 ctggccgtcc tggtgcggtt tcatgcttgt tcctcttggc gttcattctc ggcggccgcc
     4021 agggcgtcgg cctcggtcaa tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg
     4081 ggcgtcactt cctcgctgcg ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc
     4141 agtgcagccg cctctttcac ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg
     4201 atctgtgccg gggtgagggt agggcggggg ccaaacttca cgcctcgggc cttggcggcc
     4261 tcgcgcccgc tccgggtgcg gtcgatgatt agggaacgct cgaactcggc aatgccggcg
     4321 aacacggtca acaccatgcg gccggccggc gtggtggtgt cggcccacgg ctctgccagg
     4381 ctacgcaggc ccgcgccggc ctcctggatg cgctcggcaa tgtccagtag gtcgcgggtg
     4441 ctgcgggcca ggcggtctag cctggtcact gtcacaacgt cgccagggcg taggtggtca
     4501 agcatcctgg ccagctccgg gcggtcgcgc ctggtgccgg tgatcttctc ggaaaacagc
     4561 ttggtgcagc cggccgcgtg cagttcggcc cgttggttgg tcaagtcctg gtcgtcggtg
     4621 ctgacgcggg catagcccag caggccagcg gcggcgctct tgttcatggc gtaatgtctc
     4681 cggttctagt cgcaagtatt ctactttatg cgactaaaac acgcgacaag aaaacgccag
     4741 gaaaagggca gggcggcagc ctgtcgcgta acttaggact tgtgcgacat gtcgttttca
     4801 gaagacggct gcactgaacg tcagaagccg actgcactat agcagcggag gggttggatc
     4861 aaagtacttt aaagtacttt aaagtacttt aaagtacttt gatcccgagg ggaaccctgt
     4921 ggttggcatg cacatacaaa tggacgaacg gataaacctt ttcacgccct tttaaatatc
     4981 cgattattct aataaacgct cttttctctt aggtttaccc gccaatatat cctgtcaaac
     5041 actgatagtt taaactgaag gcgggaaacg acaatctgat ccaagctcaa gctgctctag
     5101 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     5161 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     5221 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     5281 agcggataac aatttcacac aggaaacagc tatgacatga ttacgaattc gagctcggta
     5341 cccgacgagt cagtaataaa cggcgtcaaa gtggttgcag ccggcacaca cgagtcgtgt
     5401 ttatcaactc aaagcacaaa tacttttcct caacctaaaa ataaggcaat tagccaaaaa
     5461 caactttgcg tgtaaacaac gctcaataca cgtgtcattt tattattagc tattgcttca
     5521 ccgccttagc tttctcgtga cctagtcgtc ctcgtctttt cttcttcttc ttctataaaa
     5581 caatacccaa agagctcttc ttcttcacaa ttcagatttc aatttctcaa aatcttaaaa
     5641 actttctctc aattctctct accgtgatca aggtaaattt ctgtgttcct tattctctca
     5701 aaatcttcga ttttgttttc gttcgatccc aatttcgtat atgttctttg gtttagattc
     5761 tgttaatctt agatcgaaga cgattttctg ggtttgatcg ttagatatca tcttaattct
     5821 cgattagggt ttcatagata tcatccgatt tgttcaaata atttgagttt tgtcgaataa
     5881 ttactcttcg atttgtgatt tctatctaga tctggtgtta gtttctagtt tgtgcgatcg
     5941 aatttgtaga ttaatctgag tttttctgat taacactcga gtgcgggatc ctctagaact
     6001 agtatggcct cctccgagga cgtcatcaag gagttcatgc gcttcaaggt gcgcatggag
     6061 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     6121 ggcacccaga ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac
     6181 atcctgtccc ctcagttcca gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
     6241 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     6301 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc
     6361 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
     6421 accatgggct gggaggcctc caccgagcgg atgtaccccg aggacggcgc cctgaagggc
     6481 gagatcaaga tgaggctgaa gctgaaggac ggcggccact acgacgccga ggtcaagacc
     6541 acctacatgg ccaagaagcc cgtgcagctg cccggcgcct acaagaccga catcaagctg
     6601 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgagcg cgccgagggc
     6661 cgccactcca ccggcgccca attgttaatc acaagtttgt acaaaaaagc aggcttcatg
     6721 tcgacgcgaa gacgaacttt actcaaagtt ataattcttg gagacagcgg ggttggcaaa
     6781 acatcgttga tgaatcaata tgtgaataac aagtttagtc aacagtacaa agctacgatc
     6841 ggagctgatt ttgtcactaa ggagcttcaa attgatgaca ggcttgtcac attgcaaata
     6901 tgggacactg ctgggctaga gaggtttcaa agtcttggtg ttgctttcta tagaggtgca
     6961 gattgttgtg ttcttgtcta tgatgtgaat cacttgaagt catttgaatc tctcgacaat
     7021 tggcacaacg agtttcttac acgggctagt ccacgtgacc caatggcatt cccttttata
     7081 cttcttggta ataaggttga tattgatgga ggaaatagcc gagtggtatc tgagaagaag
     7141 gctagagaat ggtgtgctga aaagggaaac atagtctatt tcgagacatc ggctaaagaa
     7201 gattacaatg tcgatgactc cttcttgtgc atcacaaaac ttgcccttgc aaatgaacgc
     7261 gaccaagata tatatttcca gccagatact ggttcggtgc ctgagcaaag aggaggttgt
     7321 gcttgctgag acccagcttt cttgtacaaa gtggtgatta agcggccatg ctagagtccg
     7381 caaaaatcac cagtctctct ctacaaatct atctctctct atttttctcc agaataatgt
     7441 gtgagtagtt cccagataag ggaattaggg ttcttatagg gtttcgctca tgtgttgagc
     7501 atataagaaa cccttagtat gtatttgtat ttgtaaaata cttctatcaa taaaatttct
     7561 aattcctaaa accaaaatcc agtgacctgc aggcatgcga cgtcgggccc tctagaggat
     7621 ccccgggtac cgcgaattat cgatcatgag cggagaatta agggagtcac gttatgaccc
     7681 ccgccgatga cgcgggacaa gccgttttac gtttggaact gacagaaccg caacgttgaa
     7741 ggagccactg agccgcgggt ttctggagtt taatgagcta agcacatacg tcagaaacca
     7801 ttattgcgcg ttcaaaagtc gcctaaggtc actatcagct agcaaatatt tcttgtcaaa
     7861 aatgctccac tgacgttcca taaattcccc tcggtatcca attagagtct catattcact
     7921 ctcaactcga tcgaggggat ctaccatgag cccagaacga cgcccggccg acatccgccg
     7981 tgccaccgag gcggacatgc cggcggtctg caccatcgtc aaccactaca tcgagacaag
     8041 cacggtcaac ttccgtaccg agccgcagga accgcaggag tggacggacg acctcgtccg
     8101 tctgcgggag cgctatccct ggctcgtcgc cgaggtggac ggcgaggtcg ccggcatcgc
     8161 ctacgcgggt ccctggaagg cacgcaacgc ctacgactgg acggccgagt cgaccgtgta
     8221 cgtctccccc cgccaccagc ggacgggact gggctccacg ctctacaccc acctgctgaa
     8281 gtccctggag gcacagggct tcaagagcgt ggtcgctgtc atcgggctgc ccaacgaccc
     8341 gagcgtgcgc atgcacgagg cgctcggata tgccccccgc ggcatgctgc gggcggccgg
     8401 cttcaagcac gggaactggc atgacgtggg tttctggcag ctggacttca gcctgccggt
     8461 gccgccccgt ccggtcctgc ccgtcaccga aatctgatga cccctagagt caagcagatc
     8521 gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga
     8581 ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga
     8641 cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga
     8701 tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt
     8761 tactagatcg accggcatgc aagctgataa ttcaattcgg cgttaattca gtacattaaa
     8821 aacgtccgca atgtgttatt aagttgtcta agcgtcaatt tgtttacacc acaatatatc
     8881 ctgccaccag ccagccaaca gctccccgac cggcagctcg gcacaaaatc accactcgat
     8941 acaggcagcc catcagtccg ggacggcgtc agcgggagag ccgttgtaag gcggcagact
     9001 ttgctcatgt taccgatgct attcggaaga acggcaacta agctgccggg tttgaaacac
     9061 ggatgatctc gcggagggta gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg
     9121 atcaattcgg gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa
     9181 gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg
     9241 cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg
     9301 ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa
     9361 cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacatcatgg gggaagcggt
     9421 gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga
     9481 accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca
     9541 cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc
     9601 tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc
     9661 tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg
     9721 cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc
     9781 cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt
     9841 ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc
     9901 gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa
     9961 tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa
    10021 ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact
    10081 tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca
    10141 gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataatg
    10201 tctagctaga aattcgttca agccgacgcc gcttcgcggc gcggcttaac tcaagcgtta
    10261 gatgcactaa gcacataatt gctcacagcc aaactatcag gtcaagtctg cttttattat
    10321 ttttaagcgt gcataataag ccctacacaa attgggagat atatcatgca tgaccaaaat
    10381 cccttaacgt gagttttcgt tccactgagc g
//
