LOCUS       sequence-197810-         411 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Can be used as PCR template for a STAgR reaction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Breunig CT, Durovic T, Neuner AM, Baumann V, Wiesbeck MF, Koferle A,
            Gotz M, Ninkovic J, Stricker SH
  TITLE     One step generation of customizable gRNA vectors for multiplex 
            CRISPR approaches through string assembly gRNA cloning (STAgR).
  JOURNAL   PLoS One. 2018 Apr 27;13(4):e0196015. doi: 
            10.1371/journal.pone.0196015. eCollection 2018.
  PUBMED    29702666
REFERENCE   2  (bases 1 to 411)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-04-27"; volume: "13(4):e0196015. doi"; pages: " 
            10.1371/journal.pone.0196015. eCollection 2018"
COMMENT     Sequence Label: STAGR_gRNAScaffold_mU6
FEATURES             Location/Qualifiers
     source          1..411
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        1..76
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        96..409
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     328..348
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
ORIGIN
        1 gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt
       61 ggcaccgagt cggtgctttt tttcggtgct tttttgatcc gacgccgcca tctctaggcc
      121 cgcgccggcc ccctcgcaca gacttgtggg agaagctcgg ctactcccct gccccggtta
      181 atttgcatat aatatttcct agtaactata gaggcttaat gtgcgataaa agacagataa
      241 tctgttcttt ttaatactag ctacatttta catgataggc ttggatttct ataagagata
      301 caaatactaa attattattt taaaaaacag cacaaaagga aactcaccct aactgtaaag
      361 taattgtgtg ttttgagact ataaatatcc cttggagaaa agccttgttt g
//
