LOCUS       sequence-197825-        1637 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Yeast endogenous mRNA tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Tutucci E, Vera M, Biswas J, Garcia J, Parker R, Singer RH
  TITLE     An improved MS2 system for accurate reporting of the mRNA life 
            cycle.
  JOURNAL   Nat Methods. 2017 Nov 13. doi: 10.1038/nmeth.4502.
  PUBMED    29131164
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2017 Nov 13. doi: 10.1038/nmeth.4502."
COMMENT     Sequence Label: pET251-pUC 12xMS2V6 Loxp KANr Loxp
FEATURES             Location/Qualifiers
     source          1..1637
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    29..62
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     gene            117..1473
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        117..460
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             461..1270
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(528..547)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1276..1473
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     protein_bind    1536..1569
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 agatctatcg atctcgacaa cccttaatat aacttcgtat aatgtatgct atacgaagtt
       61 attaggtcta gagatctgtt tagcttgcct cgtccccgcc gggtcacccg gccagcgaca
      121 tggaggccca gaataccctc cttgacagtc ttgacgtgcg cagctcaggg gcatgatgtg
      181 actgtcgccc gtacatttag cccatacatc cccatgtata atcatttgca tccatacatt
      241 ttgatggccg cacggcgcga agcaaaaatt acggctcctc gctgcagacc tgcgagcagg
      301 gaaacgctcc cctcacagac gcgttgaatt gtccccacgc cgcgcccctg tagagaaata
      361 taaaaggtta ggatttgcca ctgaggttct tctttcatat acttcctttt aaaatcttgc
      421 taggatacag ttctcacatc acatccgaac ataaacaacc atgggtaagg aaaagactca
      481 cgtttcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc
      541 tcgcgataat gtcgggcaat caggtgcgac aatctatcga ttgtatggga agcccgatgc
      601 gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat
      661 ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc attttatccg
      721 tactcctgat gatgcatggt tactcaccac tgcgatcccc ggcaaaacag cattccaggt
      781 attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg
      841 ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct
      901 cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga
      961 gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataagcttt tgccattctc
     1021 accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg
     1081 gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct
     1141 tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca
     1201 aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga
     1261 gtttttctaa tcagtactga caataaaaag attcttgttt tcaagaactt gtcatttgta
     1321 tagttttttt atattgtagt tgttctattt taatcaaatg ttagcgtgat ttatattttt
     1381 tttcgcctcg acatcatctg cccagatgcg aagttaagtg cgcagaaagt aatatcatgc
     1441 gtcaatcgta tgtgaatgct ggtcgctata ctgctgtcga ttcgatacta acgccgccat
     1501 ccagtgtcga aaacgagctc tcgagaaccc ttaatataac ttcgtataat gtatgctata
     1561 cgaagttatt aggtgatatc agatccacta gtggcctatg cggtacccag cttttgttcc
     1621 ctttagtgag ggttaat
//
