LOCUS       sequence-197864-         912 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  3rd generation lentiviral plasmid for inducible expression of sgRNA;
            derived from tet-pLKO-puro; puromycin selection. See manual for 
            detailed protocols..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Huang HT, Seo HS, Zhang T, Wang Y, Jiang B, Li Q, Buckley DL, Nabet 
            B, Roberts JM, Paulk J, Dastjerdi S, Winter GE, McLauchlan H, Moran 
            J, Bradner JE, Eck MJ, Dhe-Paganon S, Zhao JJ, Gray NS
  TITLE     MELK is not necessary for the proliferation of basal-like breast 
            cancer cells.
  JOURNAL   eLife. 2017 Sep 19;6. pii: e26693. doi: 10.7554/eLife.26693.
  PUBMED    28926338
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2017-09-19"; volume: "6. pii: e26693. doi"; pages: " 
            10.7554/eLife.26693"
COMMENT     Sequence Label: tet-pLKO-sgRNA-puro
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        85..179
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     primer_bind     96..115
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    133..151
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    161..179
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    344..461
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgatca
       61 cgagactagc ctcgagcggc cgcaatattt gcatgtcgct atgtgttctg ggaaatcacc
      121 ataaacgtga aatccctatc agtgatagag acttataagt tccctatcag tgatagagac
      181 accggttctg aacagcttct tgggaagtag tttaagagct aagctggaaa cagcatagca
      241 agtttaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt
      301 ttgaattctc gacctcgaga caaatggcag tattcatcca caattttaaa agaaaagggg
      361 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
      421 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
      481 gcagagatcc actttggccg cggctcgagg gggttggggt tgcgcctttt ccaaggcagc
      541 cctgggtttg cgcagggacg cggctgctct gggcgtggtt ccgggaaacg cagcggcgcc
      601 gaccctgggt ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat cttcgccgct
      661 acccttgtgg gccccccggc gacgcttcct gctccgcccc taagtcggga aggttccttg
      721 cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac gtctcactag taccctcgca
      781 gacggacagc gccagggagc aatggcagcg cgccgaccgc gatgggctgt ggccaatagc
      841 ggctgctcag cagggcgcgc cgagagcagc ggccgggaag gggcggtgcg ggaggcgggg
      901 tgtggggcgg ta
//
