LOCUS       sequence-198-exp       10449 bp    DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10449)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1997
REFERENCE   2  (bases 1 to 10449)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L3720
FEATURES             Location/Qualifiers
     source          1..10449
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1005..1033)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1781..1801
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     promoter        8194..8298
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8299..8367,8368..9159)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8517..8536)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9330..9918
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9819..9838
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10072..10089
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10206..10227
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10242..10247,10248..10265,10266..10272)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10280..10296
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10285..10307
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10304..10320
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10304..10320
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ccgcggtttt ggcgcgccaa atgtaaacct
       61 gtcatttctg tgtatttcag caccgcgaag agcaccataa acagctacaa caagagcacg
      121 aacgtcggct ccaacagaaa aagctgcaaa acgctggtac tcaccacatg ctctgataat
      181 tgccatttcc ttcgatttct gactttcaat tgtgatatga taacctcaaa atctccattc
      241 aaaatgaaaa ctcttaaatt taagagcttt caacaccccg cactttccca cctcatcgtt
      301 tttcccttct atcttatcaa ttttttgtat tttgtgtcag tttgctatat cctcatcacc
      361 ttttttaccg tttaatctct ctatagtatt gttcgggtgt ttcaagatga aacgttttgt
      421 gtgtagcatt tttgaaacac agcgcgaaaa atgcagaaac tatcatccac aatgcattaa
      481 gtgcactcat tgtgatgact cctaaccccc tcgccccacc agttgtctct tcacaaattc
      541 atggcacaat tcaaaaatgg ctttttgtgt tggatcttga tctcgtctct ttccttcttc
      601 tttcgtttct ctcggaccac tcattattcg aatgccatgc atttgttgat gatgcgcagc
      661 gatggtcgca cacactacaa cagatatgat ggtggctgtg cgtggtgcag agctcatgaa
      721 taagtgatgg ggcaccgaga tgtgactcct cccattattc gtcttgagtc gcatcttttg
      781 tcttggtccg ttgtcagtcg agcctcaagg cagccgttcc gccagtgatc gcctgctaag
      841 aagttgtagg tgtagaggaa gaaacgtcgg gtccaaattt caagcaacat ccgcgagatc
      901 gtggatccta tcgattcgcg gccgctgtac acccgggtgc cctatagtga gtcgtattgg
      961 taccggtaga aaaaatgagt aaaggagaag aacttttcac tggagttgtc ccaattcttg
     1021 ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg
     1081 atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc
     1141 catgggtaag tttaaacata tatatactaa ctaaccctga ttatttaaat tttcagccaa
     1201 cacttgtcac tactctcact tatggtgttc aatgcttctc gagataccca gatcatatga
     1261 aacggcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat
     1321 ttttcaaaga tgacgggaac tacaagacac gtaagtttaa acagttcggt actaactaac
     1381 catacatatt taaattttca ggtgctgaag tcaagtttga aggtgatacc cttgttaata
     1441 gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg
     1501 aatacaacta taactcacac aatgtataca tcgcagcaga caaacaaaag aatggaatca
     1561 aagttgtaag tttaaacatg attttactaa ctaactaatc tgatttaaat tttcagaact
     1621 tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa
     1681 atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat
     1741 ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt gagtttgtaa
     1801 cagctgctgg gattacacat ggcatggacg aactatacaa aagcccacaa gctttggaat
     1861 tcgctagccg gccatacaag taatccgggt tctagaagcg ctgctaaggg ggccctcgtc
     1921 gagtcggtca caatcacctg aaactccaaa ggcagccagt gaggaacgtg aagaagaaga
     1981 aaaagagtca tctgaacagg tttgattttc tttctggtca aaaagatgaa attattgatt
     2041 ttcagccaga tactcccaaa actagcagcg agaagtctgc aagtcgttca cagtcgccca
     2101 gagaatcgcg ggaagtgagc caagaggtat gtttttcaaa aatcaataac tgatcataat
     2161 ttttattgtt tggtgaattt aagaaaataa tattcgaaaa ttcctctgaa ttatcaagat
     2221 tgcagtatta atttcgagaa aaattgagat attcatagag ctattgtaaa ttttcttgat
     2281 ttcagactga aacttcggaa aatcaagaga aaatcaaaga aaaggatgac ggggatgatc
     2341 agcctggcac accgaacagc tatagaagcc gggaaacttc accagctcca aaaaggtcca
     2401 aggagaccag gtttgtcaaa agcttcctgc gattaattct catttcaatt tttcagagaa
     2461 tcagagtctc ctgaaaaatc cccggttcgt tcaagatctc ccagaaggtc ttcagcacgt
     2521 tccccgtcac gatctcctag acggcgccga gaaagaagct cagaaagaaa gcaatccgaa
     2581 gagccagcac cgctaccaga gaaaaagaag aaagagccgc tggatattct acgaacaaga
     2641 accggaggag catatattcc acccgccaaa cttcgactta tgcaacaaca gattagtgat
     2701 aagcaaagtg aacagtatca gagaatgaat tgggaaagaa tgaagaaaaa gattcacgga
     2761 ttggttaaca gagtcaacgc gaagaatctt gttcaaattg tcagagaact tcttcaagag
     2821 aatgtgattc gttcaaagtg agtgagaaaa tcgaaggaaa aggaaagaat taatttaatt
     2881 tttcagggga cttctctgcc gtgacattat tcaagctcag gctttctcac caggattctc
     2941 taacgtctat gcagctttgg cggcagttat caactcgaaa ttccctcatg tcggtgaact
     3001 tcttctccgt cgtctgattg tacagttcaa aagaagtttc cgtagaaatg acagaggcgt
     3061 cacggtgaac gtgatcaaat tcatcgcaca tttgattaat caacaagttg ctcacgaagt
     3121 tcttgcgctg gaaatcatga ttctgatgct tgaagaacca actgatgatt cagttgaagt
     3181 cgccattgcg ttcctgaaag agtgtggagc aaagcttctg gagattgctc cagcagctct
     3241 taacagtgtc tacgaccgtc ttcgtgcaat tctcatggaa actgaaagat cggaaaatgc
     3301 actggatcga cgtattcagt atatgattga gactgcaatg cagattcgaa aggacaaatt
     3361 tgcggtaagg tagaatatat aaatagttta ttagaaaaaa ataaattaga ataatttaaa
     3421 ttcctactag ccaatcaggc gacctttttg cgcatagttc tattattgaa aaatttggag
     3481 aatttctcat attctcgctc ggaaatctgg aattcgacga gatcttctgg cttctgtgca
     3541 gctgcatcgc tttgtgctcc ctttctcgct tgtcttctgt gtacaccaag aaccttgttg
     3601 agttcatcaa ctgaatctgt gactggcttg ttgctcactg gatgcactag acgactgatt
     3661 ctcgagaaat cagattgagt tgcgattagg gtgacctaga aattgggaat aatacgaact
     3721 tttgaaaata ttcaggagga ttaaaaaaat tattctcgac aatcctacaa atttacttat
     3781 tgcaccatgt tgctccaaca tttttcatta aaagttaatg aaaaaatgta gaaaatcgga
     3841 aattggcaat tttcagacca tttttaagca ttttcaaaaa aaaattgcag ctgaaataaa
     3901 tgtcattttc agataaatcg agcgattttc tgttgtctga cactagtttt tagttttaaa
     3961 aaatgttgga agaacatggt gcaataggta atttcataga atttccatgt gttttttttc
     4021 aattaaccaa ttatccaaat cttccaaact cacattttgc ggagctgggc tatcaagaat
     4081 ctgctgcagt tttataagac gagcatctct gatatcactg aaaattaatt tttaatcaaa
     4141 acttgaatat caactaaacc cacttattaa ctttctcgat cttctgtcgt tcggtacgat
     4201 gacggtgaag aagccaattg tagtagttga tttggttcaa gtcctttcgg tgttgtacgt
     4261 cagtgtcctg caatgctatt tagttataac ttaggcctaa gattcaattt aatgaagtga
     4321 ttaaatttgt tctctgaacc tcttaagatg atcttttgga ttagaaacat ataagacagg
     4381 tttacctatc tattaaaaaa cagatcaaaa tagatacgac caaatcggat aatccatgcc
     4441 tacctggcat ctaggaacgt gttcttagaa gatttcttac gtaatcgtat gaagaaataa
     4501 caatttgatc gttggccagc aaaaataggg ttttaagtgg gatagtgttt ttattagcta
     4561 accggaaaat tttatagttt ttttttgcaa gaaaccactg aaaaccccct aattgtatac
     4621 attttttgga gcagcttctg gtctttttga gcaataaaat tcgataaaac agaatttaag
     4681 tgtaaattgt tcacatttag tttctatttt atcaaatttt gttgctcaaa aacattcgaa
     4741 gctgctctaa aaaaatgcat taaaaaaggg gttttcagtg gtttttcaca ttaaaaaagc
     4801 taattttaac taaaaatcca tcatatttcc aactttgtca caacaataaa atgctggtca
     4861 aaatgtgttc gaaaaaatgt tttttttttt aatttttata atttaaaaat agttttcttt
     4921 cgctgggaca catacatttt tgggcgtaaa ttttcagttc aaatttccat ttttacaacc
     4981 ataatcataa agctacgtct gatctctctc gcacttacct gcgcctgatt cgaaagaaca
     5041 accgtagcca aaagaacaag aagaacaagc acgtagttgt ggtagtggac gttcatcacg
     5101 caatactgac caatggtcgt ggggtctcac tttccgtact attgagagag gggagactga
     5161 agatggcaat tgaggacagt gtcttcgacg cacgcatgca tccataagca taatccagga
     5221 gggatggaga gaaaaatctt gtttctaagc ccctcccttt gtaatacata cacatatcta
     5281 ataccgaaga atggctaatt gaatggacgt cagctgttgc tgtagttgcc aaggcatcat
     5341 cgatgaaata actgaaagaa agaattaaat aattattgca ggcgtatccg gcggtcattg
     5401 aagacttgga cttgattgag gaggaggatc agatcatcca tacacttaat ttggaggatg
     5461 cggttgatcc ggaaaatggg cttagtaagt gactgaccac acgcgggggg cattaattta
     5521 ataaattgaa ttccatttca gatgtgttca aactagatcc agaattcgaa aagaacgagg
     5581 aggtttatga ggagatccgt aaggaaatca ttggaaacgc cgatatttcg gatgaggatg
     5641 gtggcgacga gttggatgat gaagaagagg gtagtgatgt ggaagaggct ccgaagaaga
     5701 ctacagagat tattgataat actgatcaga attgactgct ttcagaaggt attcattttg
     5761 agttttgggc cggcaaatct gtaagttgcc ggttgccgaa aatttgctga atttgccgga
     5821 aaaaaaaatt ccggaattta tttaaaaact ttttgtaaaa attaaattaa atttgcaact
     5881 tttcagagaa gtctacctga caatgcaatc atctttggac taccaagaag ctgctcacaa
     5941 attgctgaaa atgaagattc cagacagcat gcaggtcagc gatgttgcaa agaaaaattt
     6001 tcgaccaaaa aaaccaacca atcataaaat ttaaaaaaaa actccgtttt tttctttttt
     6061 tttatacgag aaaaaccaaa aaaatgtatt tttgccaaat tctaaaatac tatccccgaa
     6121 attttcaata ttttctcttt cagaacgaac tctgcgcgat gcttgtcgat tgttgtgctc
     6181 aacagcgtac ctacgagcga ttctacggaa tgctcatcga acgtttctgc cgacttcgcc
     6241 tcgaatacca gcaatacttt gaaaagctct gccaggacac gtattccacg attcaccgaa
     6301 ttgacatcac aaaactgcgg aatttggctc gccttattgc tcatttgctc tcgacggatg
     6361 ctattgactg gaagattttg gccgatatga aaatgaccga agaggacaca acttcttctg
     6421 gcagaatcta tattaaatat atatttaatg aacttgtgga ggcgatggga atggttaaac
     6481 ttcattcgag agttactgat ccgtgagttt cctagagaga gttgttttcg tattcaattt
     6541 tccctatttt cagaactttg gctcattgct ttgttggatt attcccacga actaatccga
     6601 acagcgcacg attttcgatc aacttcttca caatgattgg attgggtggt ttgacgttgg
     6661 aacttcgtga atggctggca aagggtctca agaagaagaa gggaatgctg gatcagttga
     6721 aggccgaatc aagctcagat tcatcgtcgt cttcggattc gtcagactcg tctgattctt
     6781 cggattctga cgattcatcc gactcgtctt cagattcctc atcttcttca gaatcagagc
     6841 cagaaccacc gaagaaaaag aagaagaaga acagtgaaga gagttccaaa aagaaggaaa
     6901 aagagaatat tggtcgacgg gatcgtggag acaagagagc tgaacgtcat cgtgatcaaa
     6961 gtgtggagaa caaggacaag gatcgtcgac gtcgccagga ttctgacgaa aatcgtcggc
     7021 cagaacgagg agatgaccgc aaggatcgga gtaaagatcg tcgtcgtcaa gactcggatg
     7081 atgaggatcg gaaaggtcgt gaacgtcggg aagattcagg ggaaagacgt cgcggagatc
     7141 gggatcgacg tgatcgaaac aaggatcagg aggatcaccg tgaagatcgc cgtgaccgaa
     7201 gcaaggatcg tgaggatcga cgtgatcgcc gtcgtcatga ctctgatgat gatcgtaaaa
     7261 ctcgtcggga tagaagtgaa gagcgaggag gacgtcgtcg tgaagtggaa tcggatgatc
     7321 gacgccgacg tcgttgaatt ttcaaatttt aaatactgaa tatttgtttt ttttcctatt
     7381 atttatttat tctctttgtg ttttttttct tgctttctaa aaaattaatt caatccaaat
     7441 ctaaacattt ttttttctct ttccgtctcc caattcgtat tccgctcctc tcatctgaac
     7501 acaatgtgca agtttattta tcttctcgct ttcatttcat taggacgtgg ggggaattgg
     7561 tggaaggggg aaacacacaa aaggatgatg gaaatgaaat aaggacacac aatatgcaac
     7621 aacattcaat tcagaaatat ggaggaaggt ttaaaagaaa acataaaaat atatagagga
     7681 ggaaggaaaa ctagtaaaaa ataagcaaag aaattaggcg aacgatgaga attgtcctcg
     7741 cttggcaaat gcgaatccgt atggagaggc acgtttggcg aaggcaaatg ttcggtatgg
     7801 agatctgtaa aaatttttaa gttgaaattt ggtgttgctc ttttacaaaa ttttccgatt
     7861 ttcgcttgaa attacggtgc caggtctcga cacgtcttcc aatttttcaa attcaaaaga
     7921 gcctttaatg ggctgtagtt gctaatttct cgtttttgaa aatttttctt ccgtttaatc
     7981 gaaatttgat gtattttatt tatgattttc aataaatttc aaagaaactg gtgaaaactc
     8041 ggaaaattgt gaactacagt aatccaatcc ttaaaggcgc acacctttta aatgtccgcc
     8101 ccaatacgat atttttttaa gattcgctag agcggcccaa aaggcctttt ttttttggcg
     8161 ccgacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     8221 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     8281 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     8341 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     8401 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     8461 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     8521 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     8581 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     8641 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     8701 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     8761 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     8821 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     8881 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     8941 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     9001 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     9061 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     9121 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     9181 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     9241 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     9301 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     9361 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     9421 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     9481 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     9541 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     9601 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     9661 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     9721 cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     9781 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     9841 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     9901 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     9961 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
    10021 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
    10081 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
    10141 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
    10201 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
    10261 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
    10321 catgattacg ccaagctgta agtttaaaca tgatcttact aactaactat tctcatttaa
    10381 attttcagag cttaaaaatg gctgaaatca ctcacaacga tggatacgct aacaacttgg
    10441 aaatgaaat
//
