LOCUS       sequence-198019-         823 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  express His tagged P298L S285C hnRNPA2 LC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Ryan VH, Dignon GL, Zerze GH, Chabata CV, Silva R, Conicella AE, 
            Amaya J, Burke KA, Mittal J, Fawzi NL
  TITLE     Mechanistic View of hnRNPA2 Low-Complexity Domain Structure, 
            Interactions, and Phase Separation Altered by Mutation and Arginine 
            Methylation.
  JOURNAL   Mol Cell. 2018 Jan 17. pii: S1097-2765(17)30979-6. doi: 
            10.1016/j.molcel.2017.12.022.
  PUBMED    29358076
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2018 Jan 17. pii: S1097-2765(17)30979-6. doi: 
            10.1016/j.molcel.2017.12.022."
COMMENT     Sequence Label: hnRNPA2_LC_P298L_S285C
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             66..83
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             84..104
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             615..632
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      699..747
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tnnnnnnnct nnnnnnnntt tgtntanctt taagaaggag atataccatg ggctctgata
       61 aaattcatca tcatcatcat cacgaaaatc tttattttca aggtcatatg ggtcgtggtg
      121 gcaactttgg tttcggtgat agccgcggtg gtggcggcaa cttcggtccg ggtccgggca
      181 gcaatttccg tggtggttcc gacggttacg gtagcggtcg cggctttggc gatggctata
      241 atggttacgg tggtggtccg ggcggcggca atttcggtgg tagcccgggt tacggcggtg
      301 gtcgcggcgg ttatggcggt ggcggtccag gctacggtaa ccagggtggt ggttatggtg
      361 gtggctacga taactatggc ggtggtaact atggttgcgg caattacaat gattttggca
      421 actacaacca gcaactgagc aattacaatg attttggcaa ctacaaccag caaccgagca
      481 attacggccc gatgaaatcc ggcaattttg gcggttcccg taatatgggt ggtccgtatg
      541 gtggcggtaa ctacggcccg ggtggtagcg gtggttctgg cggctatggt ggccgcagcc
      601 gttactaact cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa
      661 aggaagctga gttggctgct gccaccgctg ancaataact agcataaccc cttgggggcc
      721 tctaaacggg tcttgagggg ttttttgctg aangaggaac tatatcccng attgnnnaat
      781 gggacgcncc ctgtagngag cgcanttatg cgcnnccggt gtg
//
