LOCUS       sequence-19819-e         826 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Soriano P, Friedrich G, Lawinger P
  TITLE     Promoter interactions in retrovirus vectors introduced into 
            fibroblasts and embryonic stem cells.
  JOURNAL   J Virol. 1991 May . 65(5):2314-9.
  PUBMED    2016762
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            1991 May . 65(5):2314-9."
COMMENT     Sequence Label: PGKneobpA
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             24..824
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(87..106)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     697..716
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 gggcctttcg acctgcagcc aatatgggat cggccattga acaagatgga ttgcacgcag
       61 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
      121 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
      181 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
      241 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
      301 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
      361 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
      421 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
      481 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
      541 tgttcgccag gctcaaggcg cgcatgcccg acggcgatga tctcgtcgtg acccatggcg
      601 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
      661 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
      721 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
      781 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctg
//
