LOCUS       sequence-198388-       13414 bp    DNA     circular SYN 14-APR-2025
DEFINITION  EBNA episome plasmid for U6 promoter-driven expression of 10 gRNAs 
            targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters. Includes 
            PGK-puro selection cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13414)
  AUTHORS   Weltner J, Balboa D, Katayama S, Bespalov M, Krjutskov K, Jouhilahti
            EM, Trokovic R, Kere J, Otonkoski T
  TITLE     Human pluripotent reprogramming with CRISPR activators.
  JOURNAL   Nat Commun. 2018 Jul 6;9(1):2643. doi: 10.1038/s41467-018-05067-x.
  PUBMED    29980666
REFERENCE   2  (bases 1 to 13414)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-07-6"; volume: "9(1):2643. doi"; pages: " 
            10.1038/s41467-018-05067-x"
COMMENT     Sequence Label: GG-EBNA-OSK2M2L1-PP
FEATURES             Location/Qualifiers
     source          1..13414
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     134..153
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        134..152
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     219..235
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        236..484
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        505..580
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     612..628
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        629..877
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        899..974
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(986..1002)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1006..1022
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1023..1271
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        1293..1368
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     1400..1416
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1417..1665
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        1687..1762
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     1794..1810
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1811..2059
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        2081..2156
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    2314..2347
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     2690..2706
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2707..2955
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        2977..3052
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     3084..3100
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3101..3349
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        3371..3446
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(3458..3474)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3478..3494
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3495..3743
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        3765..3840
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     3872..3888
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3889..4137
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        4159..4234
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     4266..4282
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4283..4531
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        4553..4628
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    4786..4819
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        4880..5379
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     5312..5331
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5419..6018
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APHNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5419..5438)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             6467..8392
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGXGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     protein_bind    8560..8593
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      8780..10569
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(11138..11157)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11257..11279
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(11317..11335)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11403..11507
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11508..11576,11577..12368)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11726..11745)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12539..13127
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13028..13047
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag ctctaatacg actcactata gggagacaag cttgcatgcc tgcaggtcga
      181 ctctagagga tctcgagctc aagcttcgaa ttccccctgt aaaacgacgg ccagtgaggg
      241 cctatttccc atgattcctt catatttgca tatacgatac aaggctgtta gagagataat
      301 tggaattaat ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta
      361 ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct
      421 taccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa
      481 caccgggggg agaaactgag gcgagtttta gagctagaaa tagcaagtta aaataaggct
      541 agtccgttat caacttgaaa aagtggcacc gagtcggtgc tttttttcat ggtcatagct
      601 gtttcctcat ggtaaaacga cggccagtga gggcctattt cccatgattc cttcatattt
      661 gcatatacga tacaaggctg ttagagagat aattggaatt aatttgactg taaacacaaa
      721 gatattagta caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt
      781 aaaattatgt tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt
      841 cttggcttta tatatcttgt ggaaaggacg aaacaccgca aaacccggca gcgaggctgt
      901 tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg
      961 caccgagtcg gtgctttttt tcatggtcat agctgtttcc tggacgtaaa acgacggcca
     1021 gtgagggcct atttcccatg attccttcat atttgcatat acgatacaag gctgttagag
     1081 agataattgg aattaatttg actgtaaaca caaagatatt agtacaaaat acgtgacgta
     1141 gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa atggactatc
     1201 atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     1261 gacgaaacac cggtggctgg caggctggct ctgttttaga gctagaaata gcaagttaaa
     1321 ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt tttttcatgg
     1381 tcatagctgt ttcctccagg taaaacgacg gccagtgagg gcctatttcc catgattcct
     1441 tcatatttgc atatacgata caaggctgtt agagagataa ttggaattaa tttgactgta
     1501 aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt
     1561 gcagttttaa aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat
     1621 ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccgggca cagtgccaga
     1681 ggtctggttt tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga
     1741 aaaagtggca ccgagtcggt gctttttttc atggtcatag ctgtttcctt gttgtaaaac
     1801 gacggccagt gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc
     1861 tgttagagag ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac
     1921 gtgacgtaga aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat
     1981 ggactatcat atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt
     2041 gtggaaagga cgaaacaccg tctgtggggg acctgcactg gttttagagc tagaaatagc
     2101 aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt
     2161 tttcatggtc atagctgttt cctaacgagc ggccgcagga acccctagtg atggagttgg
     2221 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac
     2281 gcccaattcc gatcatattc aataaccctt aatataactt cgtataatgt atgctatacg
     2341 aagttattag gtctgaagag gagtttacgt ccagccaagc ttaggatctc gacctcgaaa
     2401 ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc
     2461 ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc
     2521 ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc
     2581 ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa
     2641 gtagcacgtc tcactagagg atctcgagct caagcttcga attccccctg taaaacgacg
     2701 gccagtgagg gcctatttcc catgattcct tcatatttgc atatacgata caaggctgtt
     2761 agagagataa ttggaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga
     2821 cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac
     2881 tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg
     2941 aaaggacgaa acaccggtgt cagagaccgg agttgtgttt tagagctaga aatagcaagt
     3001 taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gctttttttc
     3061 atggtcatag ctgtttcctc atggtaaaac gacggccagt gagggcctat ttcccatgat
     3121 tccttcatat ttgcatatac gatacaaggc tgttagagag ataattggaa ttaatttgac
     3181 tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta
     3241 gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgcttaccg taacttgaaa
     3301 gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg tataagtaag
     3361 gaacgcgcgc gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac
     3421 ttgaaaaagt ggcaccgagt cggtgctttt tttcatggtc atagctgttt cctggacgta
     3481 aaacgacggc cagtgagggc ctatttccca tgattccttc atatttgcat atacgataca
     3541 aggctgttag agagataatt ggaattaatt tgactgtaaa cacaaagata ttagtacaaa
     3601 atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa ttatgtttta
     3661 aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg gctttatata
     3721 tcttgtggaa aggacgaaac accggctgcc atagcaacga tggagtttta gagctagaaa
     3781 tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc
     3841 tttttttcat ggtcatagct gtttcctcca ggtaaaacga cggccagtga gggcctattt
     3901 cccatgattc cttcatattt gcatatacga tacaaggctg ttagagagat aattggaatt
     3961 aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa gtaataattt
     4021 cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcatat gcttaccgta
     4081 acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg aaacaccggg
     4141 ttcccaaagc agagggcggt tttagagcta gaaatagcaa gttaaaataa ggctagtccg
     4201 ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt tcatggtcat agctgtttcc
     4261 ttgttgtaaa acgacggcca gtgagggcct atttcccatg attccttcat atttgcatat
     4321 acgatacaag gctgttagag agataattgg aattaatttg actgtaaaca caaagatatt
     4381 agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt
     4441 atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg atttcttggc
     4501 tttatatatc ttgtggaaag gacgaaacac cggcgcgcgt agttaattca tggttttaga
     4561 gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga
     4621 gtcggtgctt tttttcatgg tcatagctgt ttcctaacga gcggccgcag gaacccctag
     4681 tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa
     4741 aggtcgcccg acgcccaatt ccgatcatat tcaataaccc ttaatataac ttcgtataat
     4801 gtatgctata cgaagttatt aggtctgaag aggagtttac gtccagccaa gcttaggatc
     4861 tcgacctcga aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc
     4921 gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat
     4981 tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct
     5041 tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg
     5101 tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa
     5161 tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg
     5221 gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg
     5281 gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct
     5341 gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcatcca tctagatctc
     5401 gagcagctga agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     5461 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     5521 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     5581 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     5641 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     5701 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     5761 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     5821 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     5881 ggggtgcccg ccttcctgga gacctccgcg ccccacaacc tccccttcta cgagcggctc
     5941 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     6001 cgcaagcccg gtgcctgacg cccgccccac gacccgcagc gcccgaccga aaggagcgca
     6061 cgaccccatg catcgataag ctgatcctca caggccgcac ccagcttttc ttccgttgcc
     6121 ccagtagcat ctctgtctgg tgaccttgaa gaggaagagg aggggtcccg agaatcccca
     6181 tccctaccgt ccagcaaaaa gggggacgag gaatttgagg cctggcttga ggctcaggac
     6241 gcaaatcttg aggatgttca gcgggagttt tccgggctgc gagtaattgg tgatgaggac
     6301 gaggatggtt cggaggatgg ggaattttca gacctggatc tgtctgacag cgaccatgaa
     6361 ggggatgagg gtgggggggc tgttggaggg ggcaggagtc tgcactccct gtattcactg
     6421 agcgtcgtct aataaagatg tctattgatc tcttttagtg tgaatcatgt ctgacgaggg
     6481 gccaggtaca ggacctggaa atggcctagg agagaaggga gacacatctg gaccagaagg
     6541 ctccggcggc agtggacctc aaagaagagg gggtgataac catggacgag gacggggaag
     6601 aggacgagga cgaggaggcg gaagaccagg agccccgggc ggctcaggat cagggccaag
     6661 acatagagat ggtgtccgga gaccccaaaa acgtccaagt tgcattggct gcaaagggac
     6721 ccacggtgga acaggagcag gagcaggagc gggaggggca ggagcaggag gggcaggagc
     6781 aggaggaggg gcaggagcag gaggaggggc aggaggggca ggaggggcag gaggggcagg
     6841 agcaggagga ggggcaggag caggaggagg ggcaggaggg gcaggagggg caggagcagg
     6901 aggaggggca ggagcaggag gaggggcagg aggggcagga gcaggaggag gggcaggagg
     6961 ggcaggaggg gcaggagcag gaggaggggc aggagcagga ggaggggcag gaggggcagg
     7021 agcaggagga ggggcaggag gggcaggagg ggcaggagca ggaggagggg caggagcagg
     7081 aggggcagga ggggcaggag gggcaggagc aggaggggca ggagcaggag gaggggcagg
     7141 aggggcagga ggggcaggag caggaggggc aggagcagga ggggcaggag caggaggggc
     7201 aggagcagga ggggcaggag gggcaggagc aggaggggca ggaggggcag gagcaggagg
     7261 ggcaggaggg gcaggagcag gaggaggggc aggaggggca ggagcaggag gaggggcagg
     7321 aggggcagga gcaggagggg caggaggggc aggagcagga ggggcaggag gggsaggagc
     7381 aggaggggca ggaggggcag gagcaggagg aggggcagga gcaggagggg caggagcagg
     7441 aggtggaggc cggggtcgag gaggcagtgg aggccggggt cgaggaggta gtggaggccg
     7501 gggtcgagga ggtagtggag gccgccgggg tagaggacgt gaaagagcca gggggggaag
     7561 tcgtgaaaga gccaggggga gaggtcgtgg acgtggagaa aagaggccca ggagtcccag
     7621 tagtcagtca tcatcatccg ggtctccacc gcgcaggccc cctccaggta gaaggccatt
     7681 tttccaccct gtaggggaag ccgattattt tgaataccac caagaaggtg gcccagatgg
     7741 tgagcctgac gtgcccccgg gagcgataga gcagggcccc gcagatgacc caggagaagg
     7801 cccaagcact ggaccccggg gtcagggtga tggaggcagg cgcaaaaaag gagggtggtt
     7861 tggaaagcat cgtggtcaag gaggttccaa cccgaaattt gagaacattg cagaaggttt
     7921 aagagctctc ctggctagga gtcacgtaga aaggactacc gacgaaggaa cttgggtcgc
     7981 cggtgtgttc gtatatggag gtagtaagac ctccctttac aacctaaggc gaggaactgc
     8041 ccttgctatt ccacaatgtc gtcttacacc attgagtcgt ctcccctttg gaatggcccc
     8101 tggacccggc ccacaacctg gcccgctaag ggagtccatt gtctgttatt tcatggtctt
     8161 tttacaaact catatatttg ctgaggtttt gaaggatgcg attaaggacc ttgttatgac
     8221 aaagcccgct cctacctgca atatcagggt gactgtgtgc agctttgacg atggagtaga
     8281 tttgcctccc tggtttccac ctatggtgga aggggctgcc gcggagggtg atgacggaga
     8341 tgacggagat gaaggaggtg atggagatga gggtgaggaa gggcaggagt gatgtaactt
     8401 gttaggagac gccctcaatc gtattaaaag ccgtgtattc ccccgcacta aagaataaat
     8461 ccccagtaga catcatgcgt gctgttggtg tatttctggc catctgtctt gtcaccattt
     8521 tcgtcctccc aacatggggc aattgccgga acccttaata taacttcgta taatgtatgc
     8581 tatacgaagt tattaggtcc ctcgaagagg ttcactagcg gatctcaatt gggcataccc
     8641 atgttgtcac gtcactcagc tccgcgctca acaccttctc gcgttggaaa acattagcga
     8701 catttacctg gtgagcaatc agacatgcga cggctttagc ctggcctcct taaattcacc
     8761 taagaatggg agcaaccagc aggaaaagga caagcagcga aaattcacgc ccccttggga
     8821 ggtggcggca tatgcaaagg atagcactcc cactctacta ctgggtatca tatgctgact
     8881 gtatatgcat gaggatagca tatgctaccc ggatacagat taggatagca tatactaccc
     8941 agatatagat taggatagca tatgctaccc agatatagat taggatagcc tatgctaccc
     9001 agatataaat taggatagca tatactaccc agatatagat taggatagca tatgctaccc
     9061 agatatagat taggatagcc tatgctaccc agatatagat taggatagca tatgctaccc
     9121 agatatagat taggatagca tatgctatcc agatatttgg gtagtatatg ctacccagat
     9181 ataaattagg atagcatata ctaccctaat ctctattagg atagcatatg ctacccggat
     9241 acagattagg atagcatata ctacccagat atagattagg atagcatatg ctacccagat
     9301 atagattagg atagcctatg ctacccagat ataaattagg atagcatata ctacccagat
     9361 atagattagg atagcatatg ctacccagat atagattagg atagcctatg ctacccagat
     9421 atagattagg atagcatatg ctatccagat atttgggtag tatatgctac ccatggcaac
     9481 attagcccac cgtgctctca gcgacctcgt gaatatgagg accaacaacc ctgtgcttgg
     9541 cgctcaggcg caagtgtgtg taatttgtcc tccagatcgc agcaatcgcg cccctatctt
     9601 ggcccgccca cctacttatg caggtattcc ccggggtgcc attagtggtt ttgtgggcaa
     9661 gtggtttgac cgcagtggtt agcggggtta caatcagcca agttattaca cccttatttt
     9721 acagtccaaa accgcagggc ggcgtgtggg ggctgacgcg tgcccccact ccacaatttc
     9781 aaaaaaaaga gtggccactt gtctttgttt atgggcccca ttggcgtgga gccccgttta
     9841 attttcgggg gtgttagaga caaccagtgg agtccgctgc tgtcggcgtc cactctcttt
     9901 ccccttgtta caaatagagt gtaacaacat ggttcacctg tcttggtccc tgcctgggac
     9961 acatcttaat aaccccagta tcatattgca ctaggattat gtgttgccca tagccataaa
    10021 ttcgtgtgag atggacatcc agtctttacg gcttgtcccc accccatgga tttctattgt
    10081 taaagatatt cagaatgttt cattcctaca ctagtattta ttgcccaagg ggtttgtgag
    10141 ggttatattg gtgtcatagc acaatgccac cactgaaccc cccgtccaaa ttttattctg
    10201 ggggcgtcac ctgaaacctt gttttcgagc acctcacata caccttactg ttcacaactc
    10261 agcagttatt ctattagcta aacgaaggag aatgaagaag caggcgaaga ttcaggagag
    10321 ttcactgccc gctccttgat cttcagccac tgcccttgtg actaaaatgg ttcactaccc
    10381 tcgtggaatc ctgaccccat gtaaataaaa ccgtgacagc tcatggggtg ggagatatcg
    10441 ctgttcctta ggaccctttt actaacccta attcgatagc atatgcttcc cgttgggtaa
    10501 catatgctat tgaattaggg ttagtctgga tagtatatac tactacccgg gaagcatatg
    10561 ctacccgttt agggttaaca agggggcctt ataaacacta ttgctaatgc cctcttgagg
    10621 gtccgcttat cggtagctac acaggcccct ctgattgacg ttggtgtagc ctcccgtagt
    10681 cttcctgggc ccctgggagg tacatgtccc ccagcattgg tgtaagagct tcagccaaga
    10741 gttacacata aaggcaatgt tgtgttgcag tccacagact gcaaagtctg ctccaggatg
    10801 aaagccactc agtgttggca aatgtgcaca tccatttata aggatgtcaa ctacagtcag
    10861 agaacccctt tgtgtttggt ccccccccgt gtcacatgtg gaacagggcc cagttggcaa
    10921 gttgtaccaa ccaactgaag ggattacatg cactgccccg cgaagaaggg gcagagatgt
    10981 cgtagtcagg tttagttcgt ccggggcggg gatcgatcct ctagtaaggg attttgccga
    11041 tttcggtcta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
    11101 aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat
    11161 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
    11221 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
    11281 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
    11341 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
    11401 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
    11461 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
    11521 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
    11581 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
    11641 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
    11701 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
    11761 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
    11821 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
    11881 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
    11941 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
    12001 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
    12061 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
    12121 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
    12181 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
    12241 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
    12301 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
    12361 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
    12421 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
    12481 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
    12541 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
    12601 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
    12661 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
    12721 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
    12781 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
    12841 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
    12901 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
    12961 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
    13021 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
    13081 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
    13141 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
    13201 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
    13261 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
    13321 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
    13381 ccgactggaa agcgggcagt gagcgcaacg caat
//
