LOCUS       sequence-198468-       11523 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Level 2 construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11523)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 11523)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-153
FEATURES             Location/Qualifiers
     source          1..11523
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..467
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(99..118)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     309..330
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(560..1375)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(560..1375)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1283..1302
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1367..1386
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      1497..2085
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1986..2005
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2239..2256
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2271..2413
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2339..2358)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2499..2521
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2515..2706)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3515..4597)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     4559..4578
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(4598..4675)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     4881..4900
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     4984..5003
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4984..5002
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5003..5027
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             5081..5098
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             8652..8669
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             8939..9592
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     CDS             9599..9616
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(11437..11455)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      11451..11498
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
       61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
      361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt
      481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      541 tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat
      601 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa
      661 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc
      721 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga
      781 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc
      841 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac
      901 cgttattcat tcgtgattgc gcctgagcga gtcgaaatac gcgatcgctg ttaaaaggac
      961 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat
     1021 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag
     1081 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca
     1141 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac
     1201 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg
     1261 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca
     1321 tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac
     1381 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa
     1441 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1501 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1561 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1621 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     1681 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1741 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1801 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1861 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1921 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1981 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     2041 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     2101 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     2161 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     2221 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     2281 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta
     2341 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
     2401 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     2461 gctcccggca tccgcttaca gacaagctgt gaccgactcc gggagctgca tgtgtcagag
     2521 gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc
     2581 gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag
     2641 aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt
     2701 ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa
     2761 acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg
     2821 ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg
     2881 tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc
     2941 tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta
     3001 cgaaacacgg aaacccaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca
     3061 gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc
     3121 ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc
     3181 catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa
     3241 ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc
     3301 gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac
     3361 gagttgcatg ataaagaaca cagtcataag tgcggcgacg atagtcatgc cccgcgccca
     3421 ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta
     3481 atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     3541 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     3601 tgggcgccag ggtggttttt cttttcacca gtgacacggg caacagctga ttgcccttca
     3661 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa
     3721 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcctcg gtatcgtcgt
     3781 atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg
     3841 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca
     3901 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta
     3961 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg
     4021 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat
     4081 gctccacgcc cagtcgcgta ccgtcctcat gggagaaaat aatactgttg atgggtgtct
     4141 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg
     4201 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat
     4261 tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc
     4321 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca
     4381 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg
     4441 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt
     4501 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg
     4561 catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct
     4621 cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga
     4681 tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg
     4741 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc
     4801 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg
     4861 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg
     4921 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga
     4981 aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa
     5041 ttttgcctta actttaagaa ggagatatac catgggcagc caccaccacc accaccacag
     5101 cggaatgcag atctttgtta agactctcac cggaaagaca atcaccctgg aggtggaaag
     5161 ctccgacacc atcgacaacg ttaaggccaa gatccaggat aaggagggca ttcctccgga
     5221 tcagcagagg cttattttcg ccggcaagca gctagaggat ggccgtacgt tggctgatta
     5281 caatatccag aaggaatcca ccctccactt ggtcctcagg ctccgtggtg gttaagcttg
     5341 caattaactt taagaaggag atatacaatg cttcacaagc gagctagtga agctaacgat
     5401 aagaacgata ataccatcat cggttccgat ttagcatcat ctaagaaacg ccgtatcgat
     5461 ttcactgaat cttcgtctga taagtcatct tcgattttag cttctggtag tagcaggggt
     5521 tttcacggcg atagcgtcgt tcagcaaatc gacatggctt ttggtaattc gaaccgtcag
     5581 gagattgatg aagatctgca cagtcgccag ctcgccgtct atggtcgtga gactatgagg
     5641 cgtctctttg cttccaatgt tctcatctcg gggatgcacg gtcttggtgc cgagattgcc
     5701 aagaatctta tacttgctgg tgtgaagtct gtgaccttgc atgatgaaag agttgtagag
     5761 ctatgggact tatcaagcaa ctttgttttc tcggaggatg atgttggcaa gaatagagca
     5821 gatgcttctg ttcagaagtt acaggatctt aacaatgctg tggttgtctc tagcttgacc
     5881 aaaagcttaa acaaagagga tctttctggc ttccaggttg ttgtattctc tgacataagc
     5941 atggaaagag caatcgaatt tgatgactat tgtcacagcc atcagcctcc tatcgctttt
     6001 gtcaaggctg atgtcagggg tcttttcggc tctgtctttt gtgattttgg gcctgaattt
     6061 gcggttcttg atgttgatgg agaggaaccc catacaggca tcattgcgtc tatctctaat
     6121 gagaaccagg catttatttc ctgtgtcgac gatgagaggc ttgagtttga agatggtgac
     6181 ctagttgttt tctcagaagt tgagggtatg acagaattga atgatgggaa accaaggaag
     6241 ataaaaagta cacggccata ttcattcacc cttgatgagg acactactaa ctatggaaca
     6301 tatgtgaagg gtggtattgt cactcaagtg aaacagccaa agttactgaa tttcaagcca
     6361 ttgagggaag cgcttaagga tccaggggat tttctattta gtgatttctc taagtttgat
     6421 cggcctccac tccttcactt agcattccag gcacttgatc attttaaagc tgaagctggc
     6481 agatttcctg ttgctggctc agaagaggac gctcagaagc ttatatcgat tgccacagcc
     6541 atcaataccg ggcagggtga tttaaaggtg gagaatgttg atcagaagct tctaagacac
     6601 ttttcctttg gagccaaggc tgtcctgaat cctatggctg caatgtttgg cggtattgtt
     6661 ggacaggagg ttgttaaagc ttgctctgga aaatttcatc ctctcttcca gtttttctac
     6721 tttgattcag tggagtcact cccctctgaa cctgtggact cgagtgactt tgcaccaaga
     6781 aacagccggt atgatgccca aatatctgta tttggggcca agttccagaa gaaacttgaa
     6841 gatgctaaag ttttcacagt agggtctggt gctcttggct gtgagttctt gaaaaatttg
     6901 gctctgatgg gggtttcttg tggaagccaa gggaagctga cagtgacaga tgatgatatc
     6961 attgagaaga gtaacctcag tcgtcaattt ctgttccgtg actggaacat tggacaggct
     7021 aaatccacag ttgctgcttc ggctgctgct gttataaatc ctcgcttcaa cattgaggcc
     7081 ctgcaaaacc gtgttggcgc ggagactgag aatgtatttg acgatgcctt ctgggagaac
     7141 ttaactgttg tcgtcaatgc actggataat gttaatgcga ggctctatgt tgattcgagg
     7201 tgcttgtatt tccagaagcc tctccttgag tctggaactc ttggtacaaa gtgcaacaca
     7261 cagtcggtta tcccacatct aactgaaaat tacggtgcct caagggaccc accagagaaa
     7321 caggccccca tgtgtacagt gcattccttc ccgcataaca ttgatcactg tttaacttgg
     7381 gctcgctctg agtttgaggg tctgctggag aaaactcccg ctgaagtgaa tgcatatctt
     7441 tctagcccag ttgagtacac taactcaatg atgagtgctg gtgatgctca ggcgagggac
     7501 acattggaga ggattgttga gtgccttgaa aaggagaagt gtgaaacctt ccaagactgc
     7561 ttaacctggg cacgactgag gtttgaggat tactttgtga accgtgtcaa gcaattgata
     7621 tacacatttc ctgaagatgc tgcgactagt accggagctc cattctggtc tgctccaaaa
     7681 agattccctc gtccactcca gtactcgtcg tccgacccaa gtctccttaa ctttatcaca
     7741 gcgactgcta ttttacgagc agagacattt gggatcccga tacctgagtg gactaagaac
     7801 ccaaaggaag cagcagaagc tgttgacaga gtgatagtcc cagactttga gccaaggcaa
     7861 gatgcaaaga ttgtgactga tgagaaagct accactttaa ccactgcttc ggtggacgac
     7921 gctgcagtca tcgacgatct cattgctaag atcgaccagt gtaggcataa cttgtcgcca
     7981 gacttcagga tgaaacctat tcagttcgaa aaggatgatg atacaaacta tcacatggat
     8041 gtgatagcag gactagccaa catgagggcg agaaattaca gcatccctga agtggacaag
     8101 ctgaaagcaa agttcatagc agggagaatc atcccagcca ttgcgacttc aacagccatg
     8161 gctactgggc tggtctgcct cgagctgtac aaggtccttg atggaggaca taaagtggaa
     8221 gcttacagaa acacatttgc caacctggca cttccactat tctccatggc tgaaccactt
     8281 ccaccaaagg tggtgaagca ccgtgacatg gcttggacag tttgggacag atgggttctc
     8341 aagggaaacc caactcttcg tgaggtgttg cagtggctgg aggacaaagg gcttagcgca
     8401 tacagcatct cttgtggaag ctgtctactg ttcaacagta tgttcacaag gcacaaggag
     8461 aggatggaca agaaggttgt ggatctcgct cgggatgttg cgaaagtcga gttaccgcct
     8521 tatcgcaacc atcttgatgt agtggtggct tgtgaggatg aagatgacaa tgacgtcgat
     8581 attcctctcg tctctatcta cttcaggtga gcttactatt aactttaaga aggagatata
     8641 ccatgggcag ccaccaccac caccaccaca gcggaatgca gatctttgtt aagactctca
     8701 ccggaaagac aatcaccctg gaggtggaaa gctccgacac catcgacaac gttaaggcca
     8761 agatccagga taaggagggc attcctccgg atcagcagag gcttattttc gccggcaagc
     8821 agctagagga tggccgtacg ttggctgatt acaatatcca gaaggaatcc accctccact
     8881 tggtcctcag gctccgtggt ggttaagctt ttacttaact ttaagaagga gatataccat
     8941 gtcccctata ctaggttatt ggaaaattaa gggccttgtg caacccactc gacttctttt
     9001 ggaatatctt gaagaaaaat atgaagagca tttgtatgag cgcgatgaag gtgataaatg
     9061 gcgaaacaaa aagtttgaat tgggtttgga gtttcccaat cttccttatt atattgatgg
     9121 tgatgttaaa ttaacacagt ctatggccat catacgttat atagctgaca agcacaacat
     9181 gttgggtggt tgtccaaaag agcgtgcaga gatttcaatg cttgaaggag cggttttgga
     9241 tattagatac ggtgtttcga gaattgcata tagtaaagac tttgaaactc tcaaagttga
     9301 ttttcttagc aagctacctg aaatgctgaa aatgttcgaa gatcgtttat gtcataaaac
     9361 atatttaaat ggtgatcatg taacccatcc tgacttcatg ttgtatgacg ctcttgatgt
     9421 tgttttatac atggacccaa tgtgcctgga tgcgttccca aaattagttt gttttaaaaa
     9481 acgtattgaa gctatcccac aaattgataa gtacttgaaa tccagcaagt atatagcatg
     9541 gcctttgcag ggctggcaag ccacgtttgg tggtggcgac catcctccaa aatcggatct
     9601 ggttccgcgt ggatcaatgg cgggtatcaa tagagcggga ccatcgggcg cttattttgt
     9661 tggccacaca gaccccgagc cagtatcggg gcaagcacac ggatccggca gcggcgccag
     9721 ctcctcgaac agtccgcagg ttcagccgcg accctcgaat actcccccgt cgaacgcgcc
     9781 cgcaccgccg ccaaccggac gtgagaggct ttcacgatcc acggcgctgt cgcgccaaac
     9841 cagggagtgg ctggagcagg gtatgcctac agcggaggat gccagcgtgc gtcgtaggcc
     9901 acaggtgact gccgatgccg caacgccgcg tgcagaggca agacgcacgc cggaggcaac
     9961 tgccgatgcc agcgcaccgc gtagaggggc ggttgcacac gccaacagta tcgttcagca
    10021 attggtcagt gagggcgctg atatttcgca tactcgtaac atgctccgca atgcaatgaa
    10081 tggcgacgca gtcgcttttt ctcgagtaga acagaacata tttcgccagc atttcccgaa
    10141 catgcccatg catggaatca gccgagattc ggaactcgct atcgagctcc gtggggcgct
    10201 tcgtcgagcg gttcaccaac aggcggcgtc agcgccagtg aggtcgccca cgccaacacc
    10261 ggccagccct gcggcatcat catcgggcag cagtcagcgt tctttatttg gacggtttgc
    10321 ccgtttgatg gcgccaaacc agggacggtc gtcgaacact gccgcctctc agacgccggt
    10381 cgacaggagc ccgccacgcg tcaaccaaag acccatacgc gtcgacaggg ctgcgatgcg
    10441 taatcgtggc aatgacgagg cggacgccgc gctgcggggg ttagtacaac agggggtcaa
    10501 tttagagcac ctgcgcacgg cccttgaaag acatgtaatg cagcgcctcc ctatccccct
    10561 cgatataggc agcgcgttgc agaatgtggg aattaaccca agtatcgact tgggggaaag
    10621 ccttgtgcaa catcccctgc tgaatttgaa tgtagcgttg aatcgcatgc tggggctgcg
    10681 tcccagcgct gaaagagcgc ctcgtccagc cgtccccgtg gctcccgcga ccgcctccag
    10741 gcgaccggat ggtacgcgtg caacacgatt gcgggtgatg ccggagcggg aggattacga
    10801 aaataatgtg gcttatggag tgcgcttgct taacctgaac ccgggggtgg gggtaaggca
    10861 ggctgttgcg gcctttgtaa ccgaccgggc tgagcggcca gcagtggtgg ctaatatccg
    10921 ggcagccctg gaccctatcg cgtcacaatt cagtcagctg cgcacaattt cgaaggccga
    10981 tgctgaatct gaagagctgg gttttaagga tgcggcagat catcacacgg atgacgtgac
    11041 gcactgtctt tttggcggag aattgtcgct gagtaatccg gatcagcagg tgatcggttt
    11101 ggcgggtaat ccgacggaca cgtcgcagcc ttacagccaa gagggaaata aggacctggc
    11161 gttcatggat atgaaaaaac ttgcccaatt cctcgcaggc aagcctgagc atccgatgac
    11221 cagagaaacg cttaacgccg aaaatatcgc caagtatgct tttagaatag tcccctgagc
    11281 ttcagagctt ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
    11341 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gggatgagat ccggctgcta
    11401 acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac
    11461 cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg
    11521 gat
//
