LOCUS       sequence-199197-        4950 bp    DNA     circular SYN 14-APR-2025
DEFINITION  gene cloning of human CD1C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4950)
  AUTHORS   Lepore M, de Lalla C, Gundimeda SR, Gsellinger H, Consonni M, 
            Garavaglia C, Sansano S, Piccolo F, Scelfo A, Haussinger D, Montagna
            D, Locatelli F, Bonini C, Bondanza A, Forcina A, Li Z, Ni G, Ciceri 
            F, Jeno P, Xia C, Mori L, Dellabona P, Casorati G, De Libero G
  TITLE     A novel self-lipid antigen targets human T cells against CD1c(+) 
            leukemias.
  JOURNAL   J Exp Med. 2014 Jun 30;211(7):1363-77. doi: 10.1084/jem.20140410. 
            Epub 2014 Jun 16.
  PUBMED    24935257
REFERENCE   2  (bases 1 to 4950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1084/jem.20140410"; journalName: "J Exp Med"; date: "2014-06-30-
            30"; volume: "211"; issue: "7"; pages: "1363-77"
COMMENT     Sequence Label: pHCD1C
FEATURES             Location/Qualifiers
     source          1..4950
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1182..1198)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1182..1198)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1195..1217)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1206..1222
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1230..1236,1237..1254,1255..1260))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1275..1296
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1413..1430)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1584..2172)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1664..1683)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2343..3134,3135..3203))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2966..2985
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(3221..4015)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3332..3351)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3942..3961
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     rep_origin      complement(4359..4787)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4469..4490)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4681..4700
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4913..4935
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4927..4944
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4928..4944
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 taatacgact cactataggg cgaattgggc cctctagatg catgctcgag cggccgccag
       61 tgtgatggat atctgcagaa ttcgcccttc tcgaggacat gctgtttctg cagtttctgc
      121 tgctagctct tcttctccca ggtggtgaca atgcagacgc atcccaggaa cacgtctcct
      181 tccatgtcat ccagatcttc tcatttgtca accaatcctg ggcacgaggt cagggctcag
      241 gatggctgga cgagttgcag actcatggct gggacagtga atcaggcaca ataattttcc
      301 tgcataactg gtccaagggc aacttcagca atgaagagtt gtcagaccta gagttgttat
      361 ttcgtttcta cctctttgga ttaactcggg agattcaaga ccatgcaagt caagattact
      421 cgaaatatcc ctttgaagta caggtgaaag cgggctgtga gctgcattct ggaaagagcc
      481 cagaaggctt ctttcaggta gctttcaacg gattagattt actgagtttc cagaatacaa
      541 catgggtgcc atctccaggc tgtggaagtt tggcccaaag tgtctgtcat ctactcaatc
      601 atcagtatga aggcgtcaca gaaacagtgt ataatctcat aagaagcact tgcccccgat
      661 ttctcttggg tctcctggat gcagggaaga tgtatgtaca caggcaagtg aggccagaag
      721 cctggctgtc cagtcgcccc agccttgggt ctggccagct gttgctggtt tgtcatgcct
      781 ccggcttcta cccaaagcct gtttgggtga catggatgcg gaatgaacag gagcaactgg
      841 gcactaaaca tggtgatatt cttcctaatg ctgatgggac atggtatctt caggtgatcc
      901 tggaggtggc atctgaggag cctgctggcc tgtcttgtcg agtgagacac agcagtctag
      961 gaggccagga catcatcctc tactggggac accacttttc catgaattgg attgccttgg
     1021 tagtgatagt gcccttggtg attctaatag tccttgtgtt atggtttaag aagcactgct
     1081 catatcagga catcctgtga gcggccgcaa gggcgaattc cagcacactg gcggccgtta
     1141 ctagtggatc cgagctcggt accaagcttg gcgtaatcat ggtcatagct gtttcctgtg
     1201 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     1261 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     1321 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     1381 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     1441 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     1501 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     1561 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     1621 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     1681 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     1741 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     1801 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     1861 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     1921 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     1981 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     2041 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     2101 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     2161 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     2221 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     2281 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     2341 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     2401 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     2461 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     2521 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     2581 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     2641 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     2701 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     2761 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     2821 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     2881 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     2941 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     3001 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     3061 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     3121 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     3181 acacggaaat gttgaatact catactcttc ctttttcaat tcagaagaac tcgtcaagaa
     3241 ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc
     3301 ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct
     3361 gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt
     3421 ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc tcgccgtcgg
     3481 gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt
     3541 ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat
     3601 gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg
     3661 catcagccat gatggatact ttctcggcag gagcaaggtg ggatgacagg agatcctgcc
     3721 ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag
     3781 ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcctgcagtt
     3841 cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca
     3901 gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata
     3961 gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa
     4021 acgatcctca tcctgtctct tgatcagatc ttgatcccct gcgccatcag atccttggcg
     4081 gcaagaaagc catccagttt actttgcagg gcttcccaac cttaccagag ggcgccccag
     4141 ctggcaattc cggttcgctt gctgtccata aaaccgccca gtctagctat cgccatgtaa
     4201 gcccactgca agctacctgc tttctctttg cgcttgcgtt ttcccttgtc cagatagccc
     4261 agtagctgac attcatccgg ggtcagcacc gtttctgcgg actggctttc tacgtgttcc
     4321 gcttccttta gcagcccttg cgccctgaat tttgttaaaa ttcgcgttaa atttttgtta
     4381 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     4441 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     4501 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     4561 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     4621 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     4681 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     4741 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcca ttcgccattc
     4801 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
     4861 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
     4921 cgacgttgta aaacgacggc cagtgaattg
//
