LOCUS       sequence-199624-       13313 bp    DNA     circular SYN 14-APR-2025
DEFINITION  FRET/FLIM analysis in plants (stable or transient transformation).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13313)
  AUTHORS   Hecker A, Wallmeroth N, Peter S, Blatt MR, Harter K, Grefen C
  TITLE     Binary 2in1 Vectors Improve in Planta (Co)localization and Dynamic 
            Protein Interaction Studies.
  JOURNAL   Plant Physiol. 2015 Jul;168(3):776-87. doi: 10.1104/pp.15.00533. 
            Epub 2015 May 13.
  PUBMED    25971551
REFERENCE   2  (bases 1 to 13313)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.15.00533"; journalName: "Plant Physiol"; date: 
            "2015-07"; volume: "168"; issue: "3"; pages: "776-87"
COMMENT     Sequence Label: pFRETtv-2in1-CN
FEATURES             Location/Qualifiers
     source          1..13313
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        143..487
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             join(575..577,578..580,581..1291)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     protein_bind    1310..1434
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1459..1464,1465..1482,1483..1489)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1543..2202
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1609..1628)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2544..2849
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2728..2747
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2890..3014)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3040..3069
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3070..3099
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        3440..3784
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    3879..4002
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4037..4042,4043..4060,4061..4067)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4075..4091
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4080..4102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4099..4115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4099..4115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(4376..4500)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(4520..4522,4523..4525,4526..5239)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     promoter        5565..5743
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     terminator      6356..6608
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6713..6737
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7258..8049
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8295..8883
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8784..8803
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9037..9054
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9069..9209
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9135..9154)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9295..9317
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9553..9747
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9813..10886)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11315..11944)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    13275..13299
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aaactgaagg cgggaaacga caatctgatc caagctcaag ctaagctagg actagccctc
       61 tgcaggtcaa catggtggag cacgacactc tcgtctactc caagaatatc aaagatacag
      121 tctcagaaga ccagagggct attgagactt ttcaacaaag ggtaatatcg ggaaacctcc
      181 tcggattcca ttgcccagct atctgtcact tcatcgaaag gacagtagaa aaggaagatg
      241 gcttctacaa atgccatcat tgcgataaag gaaaggctat cgttcaagat gcctctaccg
      301 acagtggtcc caaagatgga cccccaccca cgaggaacat cgtggaaaaa gaagacgttc
      361 caaccacgtc ttcaaagcaa gtggattgat gtgatatctc cactgacgta agggatgacg
      421 cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg
      481 agaggacctc gagcgcgcgt atttttaaac aattaccaac aacaacaaca aacaacaaca
      541 acattacatt ttacattcta caactacatc tagtatggtg agcaagggcg aggagctgtt
      601 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      661 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agctgatctg
      721 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctgg gctacggcct
      781 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      841 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
      901 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
      961 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     1021 caacgtctat atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg
     1081 ccacaacatc gaggacggcg gcgtgcagct cgccgaccac taccagcaga acacccccat
     1141 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccaagctgag
     1201 caaggatccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     1261 gatcactctc ggcatggacg agctgtacaa gagtaacact agtcctagaa caagtttgta
     1321 caaaaaagct gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt
     1381 gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac tatggcggcc
     1441 gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg
     1501 agttaggatc cgtcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact
     1561 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
     1621 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
     1681 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
     1741 atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat
     1801 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
     1861 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
     1921 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca
     1981 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc
     2041 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg
     2101 ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat
     2161 gaattacaac agtactgcga tgagtggcag ggcggggcgt aaagatctgg atccggctta
     2221 ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat
     2281 actgatatgt atacccgaag tatgtcaaaa agaggtatgc tatgaagcag cgtattacag
     2341 tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg
     2401 tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc
     2461 ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc
     2521 tgacgagaac aggggctggt gaaatgcagt ttaaggttta cacctataaa agagagagcc
     2581 gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg
     2641 tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg
     2701 tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg
     2761 tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg
     2821 ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg
     2881 caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta
     2941 tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg ttcaactttt
     3001 ctatacaaag ttggtcaatt gttaggcggt ggatctctcg agcagaagtt gatctcagag
     3061 gaggacctcg agcagaagtt gatctcagag gaggacttat aagcggccat gctagagtcc
     3121 gcaaaaatca ccagtctctc tctacaaatc tatctctctc tatttttctc cagaataatg
     3181 tgtgagtagt tcccagataa gggaattagg gttcttatag ggtttcgctc atgtgttgag
     3241 catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc
     3301 taattcctaa aaccaaaatc cagtgaaggg ctcgcacgtg cggagctccg taccctctgc
     3361 aggtcaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct
     3421 cagaagacca gagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg
     3481 gattccattg cccagctatc tgtcacttca tcgaaaggac agtagaaaag gaagatggct
     3541 tctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctaccgaca
     3601 gtggtcccaa agatggaccc ccacccacga ggaacatcgt ggaaaaagaa gacgttccaa
     3661 ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac
     3721 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga
     3781 ggacctcgag cgcgcgtatt tttaaacaat taccaacaac aacaacaaac aacaacaaca
     3841 ttacatttta cattctacaa ctacatctag tcaattggca actttgtata ataaagttga
     3901 acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca
     3961 gactacataa tactgtaaaa cacaacatat ccagtcacta tggcggccgc gggctcactc
     4021 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     4081 gcggataaca atttcacaca ggaaacagct atgaccatga ttaccccgtc cctgcacgcc
     4141 tgtcgttcaa ccctggaaga tccgcgtgtc ccgagttcta acagtctggc cgtcgttctg
     4201 cagcgtcgcg attgggaaaa cccgggcgtg acccaactga atcgtctggc ggcccatccg
     4261 ccgtttgcgt cgtggcgtaa ctcagaagaa gcccgtaccg accgtccgtc tcaacaactg
     4321 cgctcctaac actataaaaa gctgatgcag acggggcttt tttctgcagg tcgaccatag
     4381 tgactggata tgttgtgttt tacagtatta tgtagtctgt tttttatgca aaatctaatt
     4441 taatatattg atatttatat cattttacgt ttctcgttca gctttcttgt acaaagtggt
     4501 gtctaggtta ggcactagta tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     4561 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     4621 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     4681 gcccgtgccc tggcccaccc tcgtgaccac cctgtcctgg ggcgtgcagt gcttcgcccg
     4741 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     4801 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     4861 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     4921 cggcaacatc ctggggcaca agctggagta caactacttt agcgacaacg tctatatcac
     4981 cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga
     5041 cggcggcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     5101 gctgctgccc gacaaccact acctgagcac ccagtccaag ctgagcaaag accccaacga
     5161 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     5221 ggacgagctg tacaagtaat tataagcggc catgctagag tccgcaaaaa tcaccagtct
     5281 ctctctacaa atctatctct ctctattttt ctccagaata atgtgtgagt agttcccaga
     5341 taagggaatt agggttctta tagggtttcg ctcatgtgtt gagcatataa gaaaccctta
     5401 gtatgtattt gtatttgtaa aatacttcta tcaataaaat ttctaattcc taaaaccaaa
     5461 atccagtgac ctaggagggt agatcatgag cggagaatta agggagtcac gttatgaccc
     5521 ccgccgatga cgcgggacaa gccgttttac gtttggaact gacagaaccg caacgttgaa
     5581 ggagccactg agcgcgggtt tctggagttt aatgagctaa gcacatacgt cagaaaccat
     5641 tattgcgcgt tcaaaagtcg cctaaggtca ctatcatcta gcaaatattt cttgtcaaaa
     5701 atgctccact gacgttccat aaattcccct cggtatccaa ttagagtctc atattcactc
     5761 tcaactcgat cgaggggatc taccatgagt ccagaaagga gaccagcaga tattaggaga
     5821 gcaaccgaag cagatatgcc agcagtttgc accattgtga accattacat cgagacttct
     5881 acagttaatt ttaggactga acctcaagag ccacaggaat ggacagatga tctcgtgaga
     5941 ttaagggaaa gatacccttg gcttgttgct gaggtggatg gagaagttgc aggtattgct
     6001 tacgcaggac cttggaaggc tagaaacgct tatgattgga ctgctgagtc taccgtttac
     6061 gtgtcaccta gacatcaaag aaccggactt ggttcaacct tgtatactca ccttttgaag
     6121 tctcttgaag ctcagggatt caaatctgtt gtggctgtta tcggtttgcc taatgatcca
     6181 agtgtgagaa tgcatgaagc tctcggatac gcaccaaggg gtatgttaag agctgctgga
     6241 tttaaacatg gtaactggca cgatgttggt ttctggcagt tagatttcag tttaccagtt
     6301 ccaccaagac cagtgcttcc agtgaccgag atttgacccg ggctagagtc aagcagatcg
     6361 ttcaaacatt tggcaataaa gtttcttaat attgaatcct gttgccggtc ttgcgatgat
     6421 tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac
     6481 gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat
     6541 agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt
     6601 actagatcga ccggcatgaa gctgagttaa cgatgtaagt cctcaattcg gcgttaattc
     6661 agtacattaa aaacgtccgc aatgtgttat taagttgtct aagcgtcaat ttgtttacac
     6721 cacaatatat cctgccacca gccagccaac agctccccga ccggcagctc ggcacaaaat
     6781 caccactcga tacaggcagc ccatcagtcc gggacggcgt cagcgggaga gccgttgtaa
     6841 ggcggcagac tttgctcatg ttaccgatgc tattcggaag aacggcaact aagctgccgg
     6901 gtttgaaaca cggatgatct cgcggagggt agcatgttga ttgtaacgat gacagagcgt
     6961 tgctgcctgt gatcaattcg ggcacgaacc cagtggacat aagcctgttc ggttcgtaag
     7021 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
     7081 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag
     7141 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta
     7201 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg
     7261 ggggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag
     7321 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc
     7381 ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca
     7441 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag
     7501 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat
     7561 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc
     7621 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat
     7681 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat
     7741 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc
     7801 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa
     7861 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag
     7921 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg
     7981 cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc
     8041 ggcaaataat gtctagctag aaattcgttc aagccgacgc cgcttcgcgg cgcggcttaa
     8101 ctcaagcgtt agatgcacta agcacataat tgctcacagc caaactatca ggtcaagtct
     8161 gcttttatta tttttaagcg tgcataataa gccctacaca aattgggaga tatatcatgc
     8221 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     8281 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     8341 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     8401 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     8461 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     8521 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     8581 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     8641 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     8701 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     8761 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     8821 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     8881 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     8941 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     9001 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     9061 gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     9121 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc
     9181 tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc
     9241 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     9301 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga
     9361 tgtgggcgcc ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg tagattgcct
     9421 ggccgtaggc cagccatttt tgagcggcca gcggccgcga taggccgacg cgaagcggcg
     9481 gggcgtaggg agcgcagcga ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc
     9541 tggccagaca gttatgcaca ggccaggcgg gttttaagag ttttaataag ttttaaagag
     9601 ttttaggcgg aaaaatcgcc ttttttctct tttatatcag tcacttacat gtgtgaccgg
     9661 ttcccaatgt acggctttgg gttcccaatg tacgggttcc ggttcccaat gtacggcttt
     9721 gggttcccaa tgtacgtgct atccacagga aagagacctt ttcgaccttt ttcccctgct
     9781 agggcaattt gccctagcat ctgctccgta cattaggaac cggcggatgc ttcgccctcg
     9841 atcaggttgc ggtagcgcat gactaggatc gggccagcct gccccgcctc ctccttcaaa
     9901 tcgtactccg gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca gaacttcttg
     9961 aactctccgg cgctgccact gcgttcgtag atcgtcttga acaaccatct ggcttctgcc
    10021 ttgcctgcgg cgcggcgtgc caggcggtag agaaaacggc cgatgccggg atcgatcaaa
    10081 aagtaatcgg ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac
    10141 atccaatcag ctagctcgat ctcgatgtac tccggccgcc cggtttcgct ctttacgatc
    10201 ttgtagcggc taatcaaggc ttcaccctcg gataccgtca ccaggcggcc gttcttggcc
    10261 ttcttcgtac gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg
    10321 tcgtctttct gctttccgcc atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt
    10381 ggacggaaca cgcggccggg cttgtctccc ttcccttccc ggtatcggtt catggattcg
    10441 gttagatggg aaaccgccat cagtaccagg tcgtaatccc acacactggc catgccggcc
    10501 ggccctgcgg aaacctctac gtgcccgtct ggaagctcgt agcggatcac ctcgccagct
    10561 cgtcggtcac gcttcgacag acggaaaacg gccacgtcca tgatgctgcg actatcgcgg
    10621 gtgcccacgt catagagcat cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc
    10681 ttcctaatcg acggcgcacc ggctgccggc ggttgccggg attctttgcg gattcgatca
    10741 gcggccgctt gccacgattc accggggcgt gcttctgcct cgatgcgttg ccgctgggcg
    10801 gcctgcgcgg ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc
    10861 gggccggatg gtttgcgacc gctcacgccg attcctcggg cttgggggtt ccagtgccat
    10921 tgcagggccg gcagacaacc cagccgctta cgcctggcca accgcccgtt cctccacaca
    10981 tggggcattc cacggcgtcg gtgcctggtt gttcttgatt ttccatgccg cctcctttag
    11041 ccgctaaaat tcatctactc atttattcat ttgctcattt actctggtag ctgcgcgatg
    11101 tattcagata gcagctcggt aatggtcttg ccttggcgta ccgcgtacat cttcagcttg
    11161 gtgtgatcct ccgccggcaa ctgaaagttg acccgcttca tggctggcgt gtctgccagg
    11221 ctggccaacg ttgcagcctt gctgctgcgt gcgctcggac ggccggcact tagcgtgttt
    11281 gtgcttttgc tcattttctc tttacctcat taactcaaat gagttttgat ttaatttcag
    11341 cggccagcgc ctggacctcg cgggcagcgt cgccctcggg ttctgattca agaacggttg
    11401 tgccggcggc ggcagtgcct gggtagctca cgcgctgcgt gatacgggac tcaagaatgg
    11461 gcagctcgta cccggccagc gcctcggcaa cctcaccgcc gatgcgcgtg cctttgatcg
    11521 cccgcgacac gacaaaggcc gcttgtagcc ttccatccgt gacctcaatg cgctgcttaa
    11581 ccagctccac caggtcggcg gtggcccata tgtcgtaagg gcttggctgc accggaatca
    11641 gcacgaagtc ggctgccttg atcgcggaca cagccaagtc cgccgcctgg ggcgctccgt
    11701 cgatcactac gaagtcgcgc cggccgatgg ccttcacgtc gcggtcaatc gtcgggcggt
    11761 cgatgccgac aacggttagc ggttgatctt cccgcacggc cgcccaatcg cgggcactgc
    11821 cctggggatc ggaatcgact aacagaacat cggccccggc gagttgcagg gcgcgggcta
    11881 gatgggttgc gatggtcgtc ttgcctgacc cgcctttctg gttaagtaca gcgataacct
    11941 tcatgcgttc cccttgcgta tttgtttatt tactcatcgc atcatatacg cagcgaccgc
    12001 atgacgcaag ctgttttact caaatacaca tcaccttttt agacggcggc gctcggtttc
    12061 ttcagcggcc aagctggccg gccaggccgc cagcttggca tcagacaaac cggccaggat
    12121 ttcatgcagc cgcacggttg agacgtgcgc gggcggctcg aacacgtacc cggccgcgat
    12181 catctccgcc tcgatctctt cggtaatgaa aaacggttcg tcctggccgt cctggtgcgg
    12241 tttcatgctt gttcctcttg gcgttcattc tcggcggccg ccagggcgtc ggcctcggtc
    12301 aatgcgtcct cacggaaggc accgcgccgc ctggcctcgg tgggcgtcac ttcctcgctg
    12361 cgctcaagtg cgcggtacag ggtcgagcga tgcacgccaa gcagtgcagc cgcctctttc
    12421 acggtgcggc cttcctggtc gatcagctcg cgggcgtgcg cgatctgtgc cggggtgagg
    12481 gtagggcggg ggccaaactt cacgcctcgg gccttggcgg cctcgcgccc gctccgggtg
    12541 cggtcgatga ttagggaacg ctcgaactcg gcaatgccgg cgaacacggt caacaccatg
    12601 cggccggccg gcgtggtggt gtcggcccac ggctctgcca ggctacgcag gcccgcgccg
    12661 gcctcctgga tgcgctcggc aatgtccagt aggtcgcggg tgctgcgggc caggcggtct
    12721 agcctggtca ctgtcacaac gtcgccaggg cgtaggtggt caagcatcct ggccagctcc
    12781 gggcggtcgc gcctggtgcc ggtgatcttc tcggaaaaca gcttggtgca gccggccgcg
    12841 tgcagttcgg cccgttggtt ggtcaagtcc tggtcgtcgg tgctgacgcg ggcatagccc
    12901 agcaggccag cggcggcgct cttgttcatg gcgtaatgtc tccggttcta gtcgcaagta
    12961 ttctacttta tgcgactaaa acacgcgaca agaaaacgcc aggaaaaggg cagggcggca
    13021 gcctgtcgcg taacttagga cttgtgcgac atgtcgtttt cagaagacgg ctgcactgaa
    13081 cgtcagaagc cgactgcact atagcagcgg aggggttgga tcaaagtact ttaaagtact
    13141 ttaaagtact ttaaagtact ttgatcccga ggggaaccct gtggttggca tgcacataca
    13201 aatggacgaa cggataaacc ttttcacgcc cttttaaata tccgattatt ctaataaacg
    13261 ctcttttctc ttaggtttac ccgccaatat atcctgtcaa acactgatag ttt
//
