LOCUS       sequence-199778-        1010 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  expresses 7D sortase A variant in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Jeong HJ, Abhiraman GC, Story CM, Ingram JR, Dougan SK
  TITLE     Generation of Ca2+-independent sortase A mutants with enhanced 
            activity for protein and cell surface labeling.
  JOURNAL   PLoS One. 2017 Dec 4;12(12):e0189068. doi: 
            10.1371/journal.pone.0189068. eCollection 2017.
  PUBMED    29200433
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2017-12-4"; volume: "12(12):e0189068. doi"; pages: " 
            10.1371/journal.pone.0189068. eCollection 2017"
COMMENT     Sequence Label: pET30b-7D-SrtA
FEATURES             Location/Qualifiers
     source          1..1010
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             482..499
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(552..570)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      566..613
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      650..1008
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(737..756)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatataca tatgcaagct aaacctcaaa ttccgaaaga
       61 taaatcaaaa gtggcaggct atattgaaat tccagatgct gatattaaag aaccagtata
      121 tccaggacca gcaacacgcg aacaattaaa tagaggtgta agctttgcaa aagaaaatca
      181 atcactagat gatcaaaata tttcaattgc aggacacact ttcattggcc gtccgaacta
      241 tcaatttaca aatcttaaag cagccaaaaa aggtagtatg gtgtacttta aagttggtaa
      301 tgaaacacgt aagtataaaa tgacaagtat aagaaacgtt aagccaacag ctgtagaagt
      361 tctagatgaa caaaaaggta aagataaaca attaacatta attacttgtg atgattacaa
      421 tgaagagaca ggcgtttggg aaacacgtaa aatctttgta gctacagaag tcaaactcga
      481 gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg aagctgagtt
      541 ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt
      601 gaggggtttt ttgctgaaag gaggaactat atccggattg gcgaatggga cgcgccctgt
      661 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
      721 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
      781 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
      841 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
      901 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg nctcttgttc
      961 caaactggna caacactcaa ccctatctcg gtctattctt ttgatttata
//
