LOCUS       sequence-200381-       11216 bp    DNA     circular SYN 14-APR-2025
DEFINITION  SCRIB WT in MSCV backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11216)
  AUTHORS   Zheng W, Umitsu M, Jagan I, Tran CW, Ishiyama N, BeGora M, Araki K, 
            Ohashi PS, Ikura M, Muthuswamy SK
  TITLE     An interaction between Scribble and the NADPH oxidase complex 
            controls M1 macrophage polarization and function.
  JOURNAL   Nat Cell Biol. 2016 Nov;18(11):1244-1252. doi: 10.1038/ncb3413. Epub
            2016 Oct 3.
  PUBMED    27694890
REFERENCE   2  (bases 1 to 11216)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb3413"; 
            journalName: "Nat Cell Biol"; date: "2016-11"; volume: "18"; issue: 
            "11"; pages: "1244-1252"
COMMENT     Sequence Label: MSCV Puro SCRIB WT
FEATURES             Location/Qualifiers
     source          1..11216
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..517
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    581..922
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             989..1405
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1335..1357
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1373..1389
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     protein_bind    1430..1454
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2918..2929
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(6351..6375)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        6392..6891
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6427..6450)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6824..6843
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6912..7511
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6912..6931)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7408..7428
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7611..8125
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    8287..8303
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8311..8317,8318..8335,8336..8341))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8356..8377
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8494..8511)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8665..9253)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8745..8764)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9424..10215,10216..10284))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10047..10066
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10285..10389)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10457..10475
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10513..10535)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10635..10654
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10848..10870
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10998..11017
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
       61 atggaaaata cataactgag aatagagaag ttcagatcaa ggttaggaac agagagacag
      121 cagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
      181 gaacagatgg tccccagatg cggtcccgcc ctcagcagtt tctagagaac catcagatgt
      241 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
      301 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
      361 ccctcactcg gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc gtattcccaa
      421 taaagcctct tgctgtttgc atccgaatcg tggactcgct gatccttggg agggtctcct
      481 cagattgatt gactgcccac ctcgggggtc tttcatttgg aggttccacc gagatttgga
      541 gacccctgcc cagggaccac cgaccccccc gccgggaggt aagctggcca gcggtcgttt
      601 cgtgtctgtc tctgtctttg tgcgtgtttg tgccggcatc taatgtttgc gcctgcgtct
      661 gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttctg
      721 aacacccggc cgcaaccctg ggagacgtcc cagggacttt gggggccgtt tttgtggccc
      781 gacctgagga agggagtcga tgtggaatcc gaccccgtca ggatatgtgg ttctggtagg
      841 agacgagaac ctaaaacagt tcccgcctcc gtctgaattt ttgctttcgg tttggaaccg
      901 aagccgcgcg tcttgtctgc tgcagcgctg cagcatcgtt ctgtgttgtc tctgtctgac
      961 tgtgtttctg tatttgtctg aaaattaggg ccagactgtt accactccct taagtttgac
     1021 cttaggtcac tggaaagatg tcgagcggat cgctcacaac cagtcggtag atgtcaagaa
     1081 gagacgttgg gttaccttct gctctgcaga atggccaacc tttaacgtcg gatggccgcg
     1141 agacggcacc tttaaccgag acctcatcac ccaggttaag atcaaggtct tttcacctgg
     1201 cccgcatgga cacccagacc aggtccccta catcgtgacc tgggaagcct tggcttttga
     1261 cccccctccc tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc ttcctccatc
     1321 cgccccgtct ctcccccttg aacctcctcg ttcgaccccg cctcgatcct ccctttatcc
     1381 agccctcact ccttctctag gcgccggaat tagatctctc gaggttatca caagtttgta
     1441 caaaaaagca ggcttaatgc tcaagtgcat cccgctgtgg cgctgcaacc ggcacgtgga
     1501 gtcggtggac aagcggcact gttcgctgca ggccgtgccg gaggagatct accgctacag
     1561 ccgcagcctg gaggagctgc tgctcgacgc caaccagctg cgcgagctgc ccaagccttt
     1621 tttccggctg ctgaacttgc gcaagctggg cctgagcgac aacgagatcc agcggttgcc
     1681 tcccgaggtg gccaacttca tgcagctggt ggagctggac gtgtcccgga acgatatccc
     1741 tgagatcccg gagagcatca agttctgcaa ggctctggag atcgcggact tcagtgggaa
     1801 ccccctctcc aggctccctg atggcttcac tcagctgcgc agcctggctc acctggccct
     1861 gaatgatgtg tctctgcagg cactgcccgg ggacgtgggc aacctcgcca acctggtgac
     1921 cctggagctc cgggagaacc tgctcaagtc cctgccagcg tccctgtcat ttctggtcaa
     1981 gctggaacag ctggatctgg gaggcaacga tctggaagtg ctgccagaca ctctgggggc
     2041 tctgcccaat cttcgggagc tgtggcttga ccggaaccag ctgtcagcac tgcccccgga
     2101 gctcgggaac ctgcggcgcc tggtgtgcct ggacgtgtcg gaaaaccggc tggaggagct
     2161 gcctgctgag ctcggcgggc tggtgctgct cactgacctg ctgctgtccc agaacctgct
     2221 gcggaggctg cccgacggca tcggtcagct gaagcagcta tccatcctaa aggtagacca
     2281 gaatcggctg tgcgaggtga ctgaggccat cggggactgt gagaacctct ctgagctgat
     2341 cctcacggag aacctgctga tggccctgcc ccgctccctg ggaaagctga ctaagctgac
     2401 caacctcaac gtggaccgga accacctcga ggcgctgccg cccgagatcg ggggctgtgt
     2461 ggcactcagc gtcctctcct tgagggacaa ccgcctggcc gtcctgccac cagagctggc
     2521 ccacacgaca gagctgcacg tgctggacgt ggcggggaac cgcctgcaga gtctgccgtt
     2581 cgcgctcacc cacctcaatc tcaaggccct gtggctggca gagaaccagg cgcagcccat
     2641 gctccggttc cagacggagg atgatgcccg gaccggcgag aaggtgctca cctgctactt
     2701 gctgccccag cagcccccac ccagcctcga ggatgctggg cagcagggga gcctctcgga
     2761 gacctggagc gatgccccgc cgagccgcgt cagcgtcatc cagttcctgg aggcccccat
     2821 aggtgatgag gacgctgagg aagctgcagc tgagaagcgg ggcctacagc gccgggccac
     2881 acctcacccc agcgagctca aggtgatgaa gaggagcatc gaggggcggc ggagcgaggc
     2941 ctgcccttgc cagccagact ctgggtcgcc cttgcctgca gaggaggaga agcggctgag
     3001 tgccgagtct ggcctgagtg aagactctcg cccatctgcc agcacagtct ctgaggctga
     3061 gcccgagggc ccgtcggctg aggcacaggg tgggagccag caggaagcca cgactgctgg
     3121 cggggaggaa gacgccgaag aggactacca ggagcccacg gtgcatttcg cagaggacgc
     3181 actgctgccc ggggatgaca gggagatcga ggaggggcag cctgaggccc cctggaccct
     3241 gccaggcggg aggcagcggc tcatccgcaa ggacacacct cactacaaaa agcacttcaa
     3301 gatctccaag ctgccccagc ccgaggccgt tgtggctctg ctgcagggca tgcagcctga
     3361 tggggagggc cctgtggctc ccgggggctg gcacaatggc ccccacgcac cctgggctcc
     3421 tcgggcccag aaggaggagg aggaggagga agagggtagt cctcaggagg aggaggaaga
     3481 ggaggaggag gaaaacaggg ctgaagagga agaggccagc actgaggagg aggacaagga
     3541 gggggccgtg gtttctgcgc cctctgtcaa gggagtgtcg tttgaccagg ccaataacct
     3601 gctgatagag cctgctcgca ttgaggagga agagctgacc ctcaccatcc tgcggcagac
     3661 tgggggcctg ggcatcagca ttgcgggcgg caagggctcc acaccctata agggggacga
     3721 cgagggcata ttcatctctc gggtgtccga ggaaggccct gcggcccggg ctggagtccg
     3781 tgtgggtgac aagctcctgg aggtgaatgg tgtggctctg cagggcgccg agcaccacga
     3841 ggccgtggag gcgctccggg gggccggcac tgccgtgcag atgcgagtgt ggcgggagcg
     3901 catggtggag cctgagaacg cggtcaccat cacgccgctg cggcccgagg atgattacag
     3961 cccccgagag cggcggggag gggggctgcg cctgcccctg ctcccgcctg agagccccgg
     4021 gcccctccgt cagcgccacg tggcctgcct ggcacgcagc gagagggggc tgggcttcag
     4081 cattgctggt gggaaaggct ccacacccta cagggctggt gatgcgggca tcttcgtctc
     4141 ccgcattgcc gagggcggtg ctgctcaccg cgcgggcaca ctgcaggttg gcgaccgcgt
     4201 cctctctatt aatggagtgg acgtgactga ggccaggcat gaccacgccg tctccctgct
     4261 gaccgctgcc tcccccacca tcgccctgct gttggagcgg gaggctgggg gccctcttcc
     4321 tcccagccct ctgccacatt cctcaccccc caccgctgct gttgccacca ccagcataac
     4381 cactgccacc cccggggtgc ctgggttgcc gagcctggcc cccagcctgc tggctgccgc
     4441 gttggaaggg ccatacccag tggaggagat ccgtctgcca agagctgggg gccctctggg
     4501 gcttagtatt gtcggaggct ccgaccattc cagccacccg tttggtgtcc aggagcctgg
     4561 tgtgttcatc tccaaggtgc tcccgcgggg cctggccgct cgcagcggcc tgcgggttgg
     4621 ggaccgcatc ctggcagtga acgggcaaga cgtgcgggat gccacgcacc aagaagcagt
     4681 cagtgccctg ctccggccct gcctggagct gtcgctgctg gtgcggaggg acccggcacc
     4741 cccgggccta cgggaactgt gcatccagaa ggcacctggg gagaggctgg gcatcagcat
     4801 ccgcgggggt gccaggggcc acgctggcaa cccccgcgac cccacagacg agggcatctt
     4861 catctccaag gtgagcccca cgggggcagc cgggcgcgac ggtcggctgc gtgtgggttt
     4921 gcggctgttg gaggtgaacc agcagagcct gctgggcctg acgcacggcg aggcggtgca
     4981 gctgctccgc agtgtgggcg acaccctcac cgtgctggtc tgtgacggct tcgaggccag
     5041 caccgacgca gccctggagg tgtccccagg tgtcattgcc aacccctttg cggcaggcat
     5101 cggccaccgg aacagcctgg agagcatctc ttccatcgac cgggagctga gccctgaggg
     5161 cccaggcaag gagaaggagc tgcctggaca gaccctgcac tgggggcccg aggccacaga
     5221 agccgcaggt cggggtctgc agcccctgaa gctggactac cgcgccctgg ccgccgtgcc
     5281 cagcgctggc agcgtgcaga gggtaccgtc tggagcagct ggagggaaga tggctgaatc
     5341 tccctgctcc cctagtggcc agcagccgcc ctccccgcct tctccggatg agctgcccgc
     5401 caatgtgaag caggcctaca gggccttcgc ggccgtgccc acttctcacc cgcctgagga
     5461 tgcccctgcc cagcccccca cgcctgggcc tgcagcctcc ccggagcagc tgtccttccg
     5521 ggagcggcag aagtactttg agctggaggt gcgcgtgccc caggccgagg gcccccctaa
     5581 gcgcgtgtcc ctggtgggtg ctgacgacct gcggaagatg caggaggagg aagccagaaa
     5641 actacagcag aagagagcgc agatgctgcg ggaggcggca gaggctgggg ccgaagcgag
     5701 gctcgccctg gacggggaga cgctgggcga ggaggaacag gaggatgagc agccaccctg
     5761 ggccagcccg agccccacct caaggcagag cccggcgtcc cccccgcccc tgggaggtgg
     5821 cgccccggtg cggacggcca aagctgaacg gcgccaccag gagcggctgc gcgtgcagag
     5881 tccggagcca ccggcacccg agcgtgccct gtcccctgcc gagctccggg ccctggaggc
     5941 cgagaagcgt gcgctgtgga gggcagccag gatgaagtca ttggaacagg acgctctccg
     6001 agcacagatg gtcctcagca ggtcccagga aggccggggc acgcgggggc ccctggagcg
     6061 actggccgag gccccttccc ctgcgcccac cccgtcgccc acccctgtgg aagaccttgg
     6121 cccccagacc agcacctccc cgggacgcct gtcaccggac tttgctgagg agttgaggtc
     6181 cctggaacca tctcccagcc ctggcccgca ggaggaggat ggagaagtgg ctctggtgct
     6241 tctgggcagg ccctcacccg gcgctgtggg ccctgaagat gtggcactgt gcagcagccg
     6301 ccgccccgta aggcctgggc gccgtggcct gggccctgtg ccctcctagt acccagcttt
     6361 cttgtacaaa gtggtgataa cgaattctac cgggtagggg aggcgctttt cccaaggcag
     6421 tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg
     6481 cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc
     6541 ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg
     6601 tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc
     6661 accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc
     6721 ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg
     6781 ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa
     6841 aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagc
     6901 ccaagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     6961 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     7021 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     7081 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
     7141 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     7201 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     7261 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     7321 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     7381 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     7441 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
     7501 ccggtgcctg acgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcacgacccc
     7561 atgcatcgat aaaataaaag attttattta gtctccagaa aaagggggga atgaaagacc
     7621 ccacctgtag gtttggcaag ctagcttaag taacgccatt ttgcaaggca tggaaaatac
     7681 ataactgaga atagagaagt tcagatcaag gttaggaaca gagagacagc agaatatggg
     7741 ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatggt
     7801 ccccagatgc ggtcccgccc tcagcagttt ctagagaacc atcagatgtt tccagggtgc
     7861 cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc
     7921 ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg
     7981 cgcgccagtc ctccgataga ctgcgtcgcc cgggtacccg tgtatccaat aaaccctctt
     8041 gcagttgcat ccgacttgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga
     8101 ctacccgtca gcgggggtct ttcatgggta acagtttctt gaagttggag aacaacattc
     8161 tgagggtagg agtcgaatat taagtaatcc tgactcaatt agccactgtt ttgaatccac
     8221 atactccaat actcctgaaa tagttcatta tggacagcgc agaaagagct ggggagaatt
     8281 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     8341 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     8401 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     8461 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     8521 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     8581 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     8641 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     8701 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     8761 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     8821 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     8881 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     8941 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     9001 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     9061 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     9121 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     9181 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     9241 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     9301 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     9361 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     9421 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     9481 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     9541 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     9601 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     9661 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     9721 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     9781 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     9841 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     9901 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     9961 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
    10021 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
    10081 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
    10141 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
    10201 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
    10261 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
    10321 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
    10381 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
    10441 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga
    10501 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
    10561 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
    10621 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
    10681 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct
    10741 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
    10801 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
    10861 ttgtaaaacg acggcgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg
    10921 gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga
    10981 gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg
    11041 ccggtgatgc cggccacgat gcgtccggcg tagaggcgat tagtccaatt tgttaaagac
    11101 aggatatcag tggtccaggc tctagttttg actcaacaat atcaccagct gaagcctata
    11161 gagtacgagc catagataaa ataaaagatt ttatttagtc tccagaaaaa gggggg
//
