LOCUS       sequence-200602-        5139 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Gateway Donor vector containing CTNNB1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5139)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 5139)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_CTNNB1_WT
FEATURES             Location/Qualifiers
     source          1..5139
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(174..201)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(293..379)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     428..450
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     442..459
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     443..459
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    475..574
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             577..2922
                     /codon_start=1
                     /gene="CTNNB1"
                     /product="protein involved both in cell-cell adhesion as 
                     part of the cadherin protein complex, and in gene 
                     transcription as part of the Wnt signaling pathway"
                     /label=CTNNB1
                     /note="also known as catenin beta-1; related to Drosophila 
                     armadillo"
                     /translation="MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPS
                     LSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLD
                     EGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDE
                     DQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLS
                     HHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQK
                     MVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTT
                     SRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGME
                     GLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDRE
                     DITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNL
                     ALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGA
                     LHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEG
                     ATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETA
                     DLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGL
                     PDLGHAQDLMDGLPPGDSNQLAWFDTDL"
     protein_bind    complement(2924..3023)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3040..3059)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3041..3059)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3064..3080)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3064..3080)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3511..4302
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4395..4983
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4884..4903
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(5137..5139,1..15)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
       61 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
      121 cgcaacgcaa ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc
      181 catccgtcag gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg
      241 cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc
      301 tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact
      361 gagcctttcg ttttatttga tgcctggcag ttccctactc tcgcgttaac gctagcatgg
      421 atgttttccc agtcacgacg ttgtaaaacg acggccagtc ttaagctcgg gccccaaata
      481 atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgatga gcaatgcttt
      541 tttataatgc caactttgta caaaaaagtt ggcaccatgg ctactcaagc tgatttgatg
      601 gagttggaca tggccatgga accagacaga aaagcggctg ttagtcactg gcagcaacag
      661 tcttacctgg actctggaat ccattctggt gccactacca cagctccttc tctgagtggt
      721 aaaggcaatc ctgaggaaga ggatgtggat acctcccaag tcctgtatga gtgggaacag
      781 ggattttctc agtccttcac tcaagaacaa gtagctgata ttgatggaca gtatgcaatg
      841 actcgagctc agagggtacg agctgctatg ttccctgaga cattagatga gggcatgcag
      901 atcccatcta cacagtttga tgctgctcat cccactaatg tccagcgttt ggctgaacca
      961 tcacagatgc tgaaacatgc agttgtaaac ttgattaact atcaagatga tgcagaactt
     1021 gccacacgtg caatccctga actgacaaaa ctgctaaatg acgaggacca ggtggtggtt
     1081 aataaggctg cagttatggt ccatcagctt tctaaaaagg aagcttccag acacgctatc
     1141 atgcgttctc ctcagatggt gtctgctatt gtacgtacca tgcagaatac aaatgatgta
     1201 gaaacagctc gttgtaccgc tgggaccttg cataaccttt cccatcatcg tgagggctta
     1261 ctggccatct ttaagtctgg aggcattcct gccctggtga aaatgcttgg ttcaccagtg
     1321 gattctgtgt tgttttatgc cattacaact ctccacaacc ttttattaca tcaagaagga
     1381 gctaaaatgg cagtgcgttt agctggtggg ctgcagaaaa tggttgcctt gctcaacaaa
     1441 acaaatgtta aattcttggc tattacgaca gactgccttc aaattttagc ttatggcaac
     1501 caagaaagca agctcatcat actggctagt ggtggacccc aagctttagt aaatataatg
     1561 aggacctata cttacgaaaa actactgtgg accacaagca gagtgctgaa ggtgctatct
     1621 gtctgctcta gtaataagcc ggctattgta gaagctggtg gaatgcaagc tttaggactt
     1681 cacctgacag atccaagtca acgtcttgtt cagaactgtc tttggactct caggaatctt
     1741 tcagatgctg caactaaaca ggaagggatg gaaggtctcc ttgggactct tgttcagctt
     1801 ctgggttcag atgatataaa tgtggtcacc tgtgcagctg gaattctttc taacctcact
     1861 tgcaataatt ataagaacaa gatgatggtc tgccaagtgg gtggtataga ggctcttgtg
     1921 cgtactgtcc ttcgggctgg tgacagggaa gacatcactg agcctgccat ctgtgctctt
     1981 cgtcatctga ccagccgaca ccaagaagca gagatggccc agaatgcagt tcgccttcac
     2041 tatggactac cagttgtggt taagctctta cacccaccat cccactggcc tctgataaag
     2101 gctactgttg gattgattcg aaatcttgcc ctttgtcccg caaatcatgc acctttgcgt
     2161 gagcagggtg ccattccacg actagttcag ttgcttgttc gtgcacatca ggatacccag
     2221 cgccgtacgt ccatgggtgg gacacagcag caatttgtgg agggggtccg catggaagaa
     2281 atagttgaag gttgtaccgg agcccttcac atcctagctc gggatgttca caaccgaatt
     2341 gttatcagag gactaaatac cattccattg tttgtgcagc tgctttattc tcccattgaa
     2401 aacatccaaa gagtagctgc aggggtcctc tgtgaacttg ctcaggacaa ggaagctgca
     2461 gaagctattg aagctgaggg agccacagct cctctgacag agttacttca ctctaggaat
     2521 gaaggtgtgg cgacatatgc agctgctgtt ttgttccgaa tgtctgagga caagccacaa
     2581 gattacaaga aacggctttc agttgagctg accagctctc tcttcagaac agagccaatg
     2641 gcttggaatg agactgctga tcttggactt gatattggtg cccagggaga accccttgga
     2701 tatcgccagg atgatcctag ctatcgttct tttcactctg gtggatatgg ccaggatgcc
     2761 ttgggtatgg accccatgat ggaacatgag atgggtggcc accaccctgg tgctgactat
     2821 ccagttgatg ggctgccaga tctggggcat gcccaggacc tcatggatgg gctgcctcca
     2881 ggtgacagca atcagctggc ctggtttgat actgacctgt aattgccaac tttcttgtac
     2941 aaagttggca ttataagaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc
     3001 agtcaaaata aaatcattat ttgccatcca gctgatatcc cctatagtga gtcgtattac
     3061 atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt
     3121 gcacaagata aaaatatatc atcatgcctc ctctagacca gccaggacag aaatgcctcg
     3181 acttcgctgc tacccaaggt tgccgggtga cgcacaccgt ggaaacggat gaaggcacga
     3241 acccagtgga cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca
     3301 cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc
     3361 atggcttgtt atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa
     3421 gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag
     3481 ggcagtcgcc ctaaaacaaa gttaaacatt atgagggaag cggtgatcgc cgaagtatcg
     3541 actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc
     3601 gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg
     3661 ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt
     3721 ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt
     3781 gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga
     3841 gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat
     3901 ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg
     3961 gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta
     4021 acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg
     4081 tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac
     4141 tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct
     4201 tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc
     4261 cactacgtga aaggcgagat caccaaggta gtcggcaaat aaccctcgag ccacccatga
     4321 ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag
     4381 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     4441 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     4501 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     4561 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     4621 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     4681 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     4741 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     4801 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     4861 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     4921 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     4981 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     5041 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     5101 gctgataccg ctcgccgcag ccgaacgacc gagcgcagc
//
