LOCUS       sequence-200691-        9541 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expresses fusion of CRY2low mutant (CRY2 aa1-491 R489E A491D) with 
            mCherry and Raf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9541)
  AUTHORS   Duan L, Hope J, Ong Q, Lou HY, Kim N, McCarthy C, Acero V, Lin MZ, 
            Cui B
  TITLE     Understanding CRY2 interactions for optical control of intracellular
            signaling.
  JOURNAL   Nat Commun. 2017 Sep 15;8(1):547. doi: 10.1038/s41467-017-00648-8.
  PUBMED    28916751
REFERENCE   2  (bases 1 to 9541)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-09-15"; volume: "8(1):547. doi"; pages: " 
            10.1038/s41467-017-00648-8"
COMMENT     Sequence Label: CRY2low-mCherry-Raf1
FEATURES             Location/Qualifiers
     source          1..9541
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(64..83)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        255..634
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        635..838
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     789..809
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2410..3117
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2555..2573)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2823..2842
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        complement(5114..5132)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5115..5132)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5152..5169)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5158..5382
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5428..5856
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5515..5534)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5725..5746
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5865..5885)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5870..6199
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6050..6185
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6112..6131
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6266..7060
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6320..6339)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6930..6949
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7234..7355
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7271..7290)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7325..7344
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7404..7420)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7404..7420)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7417..7439)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7428..7444
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7452..7458,7459..7476,7477..7482))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7497..7518
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7635..7652)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7806..8394)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7886..7905)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8565..9356,9357..9425))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9188..9207
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9426..9530)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct
       61 cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt
      121 ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga
      181 caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc
      241 cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
      301 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
      361 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
      421 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
      481 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
      541 taaatggccc gcctgacatt atgcccagta catgacctta tgggactttc ctacttggca
      601 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
      661 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
      721 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
      781 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctgg
      841 tttagtgaac cgtcagatcc gctagcgcta ccggactcag atctcgaggc caccatgaag
      901 atggacaaaa agactatagt ttggtttaga agagacctaa ggattgagga taatcctgca
      961 ttagcagcag ctgctcacga aggatctgtt tttcctgtct tcatttggtg tcctgaagaa
     1021 gaaggacagt tttatcctgg aagagcttca agatggtgga tgaaacaatc acttgctcac
     1081 ttatctcaat ccttgaaggc tcttggatct gacctcactt taatcaaaac ccacaacacg
     1141 atttcagcga tcttggattg tatccgcgtt accggtgcta caaaagtcgt ctttaaccac
     1201 ctctatgatc ctgtttcgtt agttcgggac cataccgtaa aggagaagct ggtggaacgt
     1261 gggatctctg tgcaaagcta caatggagat ctattgtatg aaccgtggga gatatactgc
     1321 gaaaagggca aaccttttac gagtttcaat tcttactgga agaaatgctt agatatgtcg
     1381 attgaatccg ttatgcttcc tcctccttgg cggttgatgc caataactgc agcggctgaa
     1441 gcgatttggg cgtgttcgat tgaagaacta gggctggaga atgaggccga gaaaccgagc
     1501 aatgcgttgt taactagagc ttggtctcca ggatggagca atgctgataa gttactaaat
     1561 gagttcatcg agaagcagtt gatagattat gcaaagaaca gcaagaaagt tgttgggaat
     1621 tctacttcac tactttctcc gtatctccat ttcggggaaa taagcgtcag acacgttttc
     1681 cagtgtgccc ggatgaaaca aattatatgg gcaagagata agaacagtga aggagaagaa
     1741 agtgcagatc tttttcttag gggaatcggt ttaagagagt attctcggta tatatgtttc
     1801 aacttcccgt ttactcacga gcaatcgttg ttgagtcatc ttcggttttt cccttgggat
     1861 gctgatgttg ataagttcaa ggcctggaga caaggcagga ccggttatcc gttggtggat
     1921 gccggaatga gagagctttg ggctaccgga tggatgcata acagaataag agtgattgtt
     1981 tcaagctttg ctgtgaagtt tcttctcctt ccatggaaat ggggaatgaa gtatttctgg
     2041 gatacacttt tggatgctga tttggaatgt gacatccttg gctggcagta tatctctggg
     2101 agtatccccg atggccacga gcttgatcgc ttggacaatc ccgcgttaca aggcgccaaa
     2161 tatgacccag aaggtgagta cataaggcaa tggcttcccg agcttgcgag attgccaact
     2221 gaatggatcc atcatccatg ggacgctcct ttaaccgtac tcaaagcttc tggtgtggaa
     2281 ctcggaacaa actatgcgaa acccattgta gacatcgaca cagctcgtga gctactagct
     2341 aaagctattt caagaaccga ggaagacgaa ttcgatagtg ctggtagtgc tggtagtgct
     2401 ggtactagta tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
     2461 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
     2521 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
     2581 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
     2641 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
     2701 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
     2761 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
     2821 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
     2881 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
     2941 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
     3001 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
     3061 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtcc
     3121 ggactcagat ctcgagtgat ggagcacata cagggagctt ggaagacgat cagcaatggt
     3181 tttggattca aagatgccgt gtttgatggc tccagctgca tctctcctac aatagttcag
     3241 cagtttggct atcagcgccg ggcatcagat gatggcaaac tcacagatcc ttctaagaca
     3301 agcaacacta tccgtgtttt cttgccgaac aagcaaagaa cagtggtcaa tgtgcgaaat
     3361 ggaatgagct tgcatgactg ccttatgaaa gcactcaagg tgaggggcct gcaaccagag
     3421 tgctgtgcag tgttcagact tctccacgaa cacaaaggta aaaaagcacg cttagattgg
     3481 aatactgatg ctgcgtcttt gattggagaa gaacttcaag tagatttcct ggatcatgtt
     3541 cccctcacaa cacacaactt tgctcggaag acgttcctga agcttgcctt ctgtgacatc
     3601 tgtcagaaat tcctgctcaa tggatttcga tgtcagactt gtggctacaa atttcatgag
     3661 cactgtagca ccaaagtacc tactatgtgt gtggactgga gtaacatcag acaactctta
     3721 ttgtttccaa attccactat tggtgatagt ggagtcccag cactaccttc tttgactatg
     3781 cgtcgtatgc gagagtctgt ttccaggatg cctgttagtt ctcagcacag atattctaca
     3841 cctcacgcct tcacctttaa cacctccagt ccctcatctg aaggttccct ctcccagagg
     3901 cagaggtcga catccacacc taatgtccac atggtcagca ccaccctgcc tgtggacagc
     3961 aggatgattg aggatgcaat tcgaagtcac agcgaatcag cctcaccttc agccctgtcc
     4021 agtagcccca acaatctgag cccaacaggc tggtcacagc cgaaaacccc cgtgccagca
     4081 caaagagagc gggcaccagt atctgggacc caggagaaaa acaaaattag gcctcgtgga
     4141 cagagagatt caagctatta ttgggaaata gaagccagtg aagtgatgct gtccactcgg
     4201 attgggtcag gctcttttgg aactgtttat aagggtaaat ggcacggaga tgttgcagta
     4261 aagatcctaa aggttgtcga cccaacccca gagcaattcc aggccttcag gaatgaggtg
     4321 gctgttctgc gcaaaacacg gcatgtgaac attctgcttt tcatggggta catgacaaag
     4381 gacaacctgg caattgtgac ccagtggtgc gagggcagca gcctctacaa acacctgcat
     4441 gtccaggaga ccaagtttca gatgttccag ctaattgaca ttgcccggca gacggctcag
     4501 ggaatggact atttgcatgc aaagaacatc atccatagag acatgaaatc caacaatata
     4561 tttctccatg aaggcttaac agtgaaaatt ggagattttg gtttggcaac agtaaagtca
     4621 cgctggagtg gttctcagca ggttgaacaa cctactggct ctgtcctctg gatggcccca
     4681 gaggtgatcc gaatgcagga taacaaccca ttcagtttcc agtcggatgt ctactcctat
     4741 ggcatcgtat tgtatgaact gatgacgggg gagcttcctt attctcacat caacaaccga
     4801 gatcagatca tcttcatggt gggccgagga tatgcctccc cagatcttag taagctatat
     4861 aagaactgcc ccaaagcaat gaagaggctg gtagctgact gtgtgaagaa agtaaaggag
     4921 gagaggcctc tttttcccca gatcctgtct tccattgagc tgctccaaca ctctctaccg
     4981 aagatcaacc ggagcgcttc cgagccatcc ttgcatcggg cagcccacac tgaggatatc
     5041 aatgcttgca cgctgaccac gtccccgagg ctgcctgtct tctaagcggc cgctcgagtc
     5101 tagagggccc tattctatag tgtcacctaa atgctagagc tcgctgatca gcctcgactg
     5161 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     5221 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     5281 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     5341 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     5401 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
     5461 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     5521 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     5581 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     5641 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     5701 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     5761 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     5821 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
     5881 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     5941 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     6001 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     6061 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     6121 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
     6181 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
     6241 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
     6301 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     6361 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     6421 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
     6481 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     6541 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     6601 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     6661 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     6721 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     6781 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     6841 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     6901 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     6961 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     7021 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
     7081 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
     7141 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     7201 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
     7261 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     7321 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
     7381 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     7441 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     7501 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     7561 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     7621 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     7681 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     7741 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     7801 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     7861 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     7921 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     7981 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     8041 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     8101 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     8161 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     8221 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     8281 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     8341 ggtggttttt ttgtttgcaa gcagcagatt acgtgcagaa aaaaaggatc tcaagaagat
     8401 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     8461 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     8521 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     8581 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     8641 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     8701 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     8761 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     8821 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     8881 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     8941 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     9001 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     9061 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     9121 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     9181 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     9241 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     9301 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     9361 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     9421 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     9481 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     9541 c
//
