LOCUS       sequence-200773-        1398 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Expression of a chimeric G-protein coupled receptor 
            (Rhodopsin-GPR22; Opto-GPR22; A12).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Morri M, Sanchez-Romero I, Tichy AM, Kainrath S, Gerrard EJ, 
            Hirschfeld PP, Schwarz J, Janovjak H
  TITLE     Optical functionalization of human Class A orphan G-protein-coupled 
            receptors.
  JOURNAL   Nat Commun. 2018 May 16;9(1):1950. doi: 10.1038/s41467-018-04342-1.
  PUBMED    29769519
REFERENCE   2  (bases 1 to 1398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-05-16"; volume: "9(1):1950. doi"; pages: " 
            10.1038/s41467-018-04342-1"
COMMENT     Sequence Label: Opto-GPR22
FEATURES             Location/Qualifiers
     source          1..1398
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..84
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             109..147
                     /codon_start=1
                     /product="segment of bovine rhodopsin containing a signal 
                     for N-linked glycosylation (Favaloro et al., 2008)"
                     /label=opsin tag
                     /note="The asparagine in the NKT tripeptide will be 
                     glycosylated if this sequence is exposed to the ER lumen."
                     /translation="GPNFYVPFSNKTG"
ORIGIN
        1 atgaaaacga tcatcgccct gagctacatc ttctgcctgg tattcgccat gtacaccgat
       61 atagagatga acaggctggg aaaggatagc ctcatgaacg ggaccgaggg cccaaacttc
      121 tacgtgcctt tctccaacaa gacgggcgtg gtgcgcagcc ccttcgaggc cccgcagtac
      181 tacctggcgg agccatggca gttctccatg ctggccgcct acatgttcct gctgatcatg
      241 cttggcttcc ccatcaactt cctcacgctg tacgtctact gcatgaaatc caacttaatc
      301 aactctgtcc tcaactacat cctgctcaac ctggccgtgg ccgacctctt catggtgttc
      361 gggggcttca ccaccaccct ctacacctct ctgcacgggt acttcgtctt tgggcccacg
      421 ggctgcaacc tggagggctt ctttgccacc ttgggcggtg aaattgcact gtggtccttg
      481 gtggtcctgg ccatcgagcg gtacgtggtg gtggtaaaac ctgcaaaccg aattctgaca
      541 atgggcagag ccatcatggg cgtcgccttc acctgggtca tggctctggc ctgtgccgcg
      601 ccccccctcg tcggctggtc caggtacatc ccggagggca tgcagtgctc gtgcgggatt
      661 gactactaca cgccccacga ggaaaccaac aatgagtcgt tcgtcatcta catgttcgtg
      721 gtccacttca tcatccccct gattgtcata ttcttctgct acggaaaaat acttcaggct
      781 cttaatattc gaataggcac aagattttca acagggcaga agaagaaagc aagaaagaaa
      841 aagacaattt ctctaaccac acaacatgag gctacagaca tgtcacaaag cagtggtggg
      901 agaaatgtag tctttggtgt aagaacttca gtttctgtaa taattgccct ccggcgagct
      961 gtgaaacgac accgtgaacg acgagaaaga caaaagagag tgttccgcat ggtgatcatc
     1021 atggtcatcg ctttcctaat ctgctggctg ccctacgctg gggtggcgtt ctacatcttc
     1081 acccatcagg gctctgactt tggccccatc ttcatgacca tcccggcttt ctttgccaag
     1141 acttctgccg tctacaaccc cgtcatctac atcatgatga acaagcagtt ccgcaaggtc
     1201 ttgaaaagta aaatgaaaaa gcgagttgtt tctatagtag aagctgatcc cctgcctaat
     1261 aatgctgtaa tacacaactc ttggatagat cctaaaagaa acaaaaaaat tacctttgaa
     1321 gatagtgaaa taagagaaaa atgtttagtg cctcaggttg tcacagacac agaaaccagc
     1381 caagtggcgc ctgcctaa
//
