LOCUS       sequence-200779-        1116 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Expression of a chimeric G-protein coupled receptor 
            (Rhodopsin-GPR33; Opto-GPR33; B6).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Morri M, Sanchez-Romero I, Tichy AM, Kainrath S, Gerrard EJ, 
            Hirschfeld PP, Schwarz J, Janovjak H
  TITLE     Optical functionalization of human Class A orphan G-protein-coupled 
            receptors.
  JOURNAL   Nat Commun. 2018 May 16;9(1):1950. doi: 10.1038/s41467-018-04342-1.
  PUBMED    29769519
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-05-16"; volume: "9(1):1950. doi"; pages: " 
            10.1038/s41467-018-04342-1"
COMMENT     Sequence Label: Opto-GPR33
FEATURES             Location/Qualifiers
     source          1..1116
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             52..84
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             109..147
                     /codon_start=1
                     /product="segment of bovine rhodopsin containing a signal 
                     for N-linked glycosylation (Favaloro et al., 2008)"
                     /label=opsin tag
                     /note="The asparagine in the NKT tripeptide will be 
                     glycosylated if this sequence is exposed to the ER lumen."
                     /translation="GPNFYVPFSNKTG"
ORIGIN
        1 atgaaaacga tcatcgccct gagctacatc ttctgcctgg tattcgccat gtacaccgat
       61 atagagatga acaggctggg aaaggatagc ctcatgaacg ggaccgaggg cccaaacttc
      121 tacgtgcctt tctccaacaa gacgggcgtg gtgcgcagcc ccttcgaggc cccgcagtac
      181 tacctggcgg agccatggca gttctccatg ctggccgcct acatgttcct gctgatcatg
      241 cttggcttcc ccatcaactt cctcacgctg tacgtcctaa gattcaagat gaaacagact
      301 gtcctcaact acatcctgct caacctggcc gtggccgacc tcttcatggt gttcgggggc
      361 ttcaccacca ccctctacac ctctctgcac gggtacttcg tctttgggcc cacgggctgc
      421 aacctggagg gcttctttgc caccttgggc ggtgaaattg cactgtggtc cttggtggtc
      481 ctggccatcg agcggtacgt ggtggtgctt cacccagtgt ggtcccagca gcaccgaacc
      541 ccgcgctggg ccatcatggg cgtcgccttc acctgggtca tggctctggc ctgtgccgcg
      601 ccccccctcg tcggctggtc caggtacatc ccggagggca tgcagtgctc gtgcgggatt
      661 gactactaca cgccccacga ggaaaccaac aatgagtcgt tcgtcatcta catgttcgtg
      721 gtccacttca tcatccccct gattgtcata ttcttctgct acggaagagt agccagcaag
      781 gtgaaagaga ggagcctgtt taaatccagc aagcccttcc gcatggtgat catcatggtc
      841 atcgctttcc taatctgctg gctgccctac gctggggtgg cgttctacat cttcacccat
      901 cagggctctg actttggccc catcttcatg accatcccgg ctttctttgc caagacttct
      961 gccgtctaca accccgtcat ctacatcatg atgaacaagc agttccgcaa ggtgttcaag
     1021 aagtccattc ttgctctgtt tgagtcaaca tttagtgaag attcttctgt agaaaggaca
     1081 caaaccacag aaaccagcca agtggcgcct gcctaa
//
