LOCUS       sequence-201035-       11031 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses TREAT-5'TOP reporter in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11031)
  AUTHORS   Horvathova I, Voigt F, Kotrys AV, Zhan Y, Artus-Revel CG, Eglinger 
            J, Stadler MB, Giorgetti L, Chao JA
  TITLE     The Dynamics of mRNA Turnover Revealed by Single-Molecule Imaging in
            Single Cells.
  JOURNAL   Mol Cell. 2017 Nov 2;68(3):615-625.e9. doi: 
            10.1016/j.molcel.2017.09.030. Epub 2017 Oct 19.
  PUBMED    29056324
REFERENCE   2  (bases 1 to 11031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2017.09.030"; journalName: "Mol Cell"; date: 
            "2017-11-2- 2"; volume: "68"; issue: "3"; pages: "615-625.e9"
COMMENT     Sequence Label: pSF4 TetCMV 5'TOP intron Renilla-STOP 24xPP7
            1...
FEATURES             Location/Qualifiers
     source          1..11031
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(12..28)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12..28)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(25..47)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    36..52
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(60..66,67..84,85..90))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    105..126
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(243..260)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(414..1002)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(494..513)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1173..1964,1965..2033))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1796..1815
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2034..2138)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2206..2224
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2262..2284)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2384..2403
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2597..2619
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2611..2628
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2612..2628
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3712..3759)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    join(3867..3885,3886..3908,3909..3927,3928..3950,3951..3969,
                     3970..3992,3993..4011,4012..4034,4035..4053,4054..4076,
                     4077..4095,4096..4118,4119..4137)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        4170..4208
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     intron          4284..4416
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     4399..4418
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        4461..4479
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4483..4492
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4489..5424
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    8829..8963
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     8907..8926
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(9157..9173)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(9189..9211)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    9622..9979
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             10044..10513
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
       61 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
      121 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
      181 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
      241 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
      301 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
      361 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
      421 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
      481 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
      541 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
      601 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
      661 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
      721 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
      781 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
      841 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
      901 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      961 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     1021 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     1081 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     1141 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     1201 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     1261 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     1321 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     1381 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     1441 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     1501 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     1561 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     1621 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     1681 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     1741 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     1801 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     1861 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     1921 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     1981 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     2041 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     2101 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     2161 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     2221 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     2281 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     2341 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
     2401 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     2461 cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     2521 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     2581 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga attaattcca
     2641 agctaagtga aagactctgt ctcaaaaacc aacctgacac ctgagccgga gcgttgcctc
     2701 tcactaccat aaatatatat acacaacaca cacacacaca cacacacaca cacacacaca
     2761 cacacacaca caatcataca cataaacaca atcacacaca cacacatgca caatcacaca
     2821 cacacaatca cacacaaaca tacatacaca caatcacaca caaccataca cacatataca
     2881 caatcacaca cacaaacaca atcacacaca cacacatcaa tcaatcaatc aatcaatata
     2941 ccaaaaatga tagctcatac ctgtaatctt agatgtggga aggctaaaac agcaggctac
     3001 ccatgacttc aaggtcaccc tgggttacag agtagctaat agtctagctt gaactatagt
     3061 gtaggatctt taaaaaaaaa ttaaccaacc aaccaacaaa caaacaaaca aacaacaaca
     3121 acaacaaatg gaagacagag gacaaaaaaa aataaaagaa aaagtagaaa gccaatggag
     3181 atactatctt aaaattagaa atatttatga cacattctga atactccatg tttatttcaa
     3241 ggaagccaat gtaactttaa gtactatggc catttaatgt aaatacttaa gaaaaaaaac
     3301 caaattaatt ttgatacatg ctgcatgtga aagacccccg ctgacgggta gtcaatcact
     3361 cagaggagac cctctcaagg aacagcgaga ccacaagtcg gatgcaactg caagagggtt
     3421 tattggatac acgggtaccc gggcgactca gtcaatcgga ggactggcgc cccgagtgag
     3481 gggttgtggg ctcgagctcg gggagcagaa gcgcgcgaac agaagtcgag ctagcttgcc
     3541 aaacctacag gtggggtctt tcattccccc ctttttctgg agactaaata aaatctttta
     3601 ttttatctat ggctcgtact ctataggctt cagctggtga tattgttgag tcaaaactag
     3661 agcctggacc actgatatcc tgtctttaac aaattggact aatccggatc tgaagttcct
     3721 atactttcta gagaatagga acttcggaat aggaacttcg cggccagatc ttttgcggcc
     3781 gctttgggcc ggccgaattc gcgcaaattt aaccctcact aaagggaaca aaagctgggt
     3841 accgggcccc ccctcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     3901 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     3961 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     4021 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
     4081 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
     4141 gtgaaagtcg agctcggtac ccgggtcgag gtaggcgtgt acggtgggag gcctatataa
     4201 gcagagctcg tttaggccac cgtcctctct tcctcggcgc tgcctacgga ggtggcagcc
     4261 atctccttct cggcaccggt caggtaagta tcaaggttac aagacaggtt taaggagacc
     4321 aatagaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag gcacctattg
     4381 gtcttactga catccacttt gcctttctct ccacaggtgt ccactcccag ttcaattaca
     4441 gctcttaagg ctagagtact taatacgact cactataggc tagccaccat ggcttccaag
     4501 gtgtacgacc ccgagcaacg caaacgcatg atcactgggc ctcagtggtg ggctcgctgc
     4561 aagcaaatga acgtgctgga ctccttcatc aactactatg attccgagaa gcacgccgag
     4621 aacgccgtga tttttctgca tggtaacgct gcctccagct acctgtggag gcacgtcgtg
     4681 cctcacatcg agcccgtggc tagatgcatc atccctgatc tgatcggaat gggtaagtcc
     4741 ggcaagagcg ggaatggctc atatcgcctc ctggatcact acaagtacct caccgcttgg
     4801 ttcgagctgc tgaaccttcc aaagaaaatc atctttgtgg gccacgactg gggggcttgt
     4861 ctggcctttc actactccta cgagcaccaa gacaagatca aggccatcgt ccatgctgag
     4921 agtgtcgtgg acgtgatcga gtcctgggac gagtggcctg acatcgagga ggatatcgcc
     4981 ctgatcaaga gcgaagaggg cgagaaaatg gtgcttgaga ataacttctt cgtcgagacc
     5041 atgctcccaa gcaagatcat gcggaaactg gagcctgagg agttcgctgc ctacctggag
     5101 ccattcaagg agaagggcga ggttagacgg cctaccctct cctggcctcg cgagatccct
     5161 ctcgttaagg gaggcaagcc cgacgtcgtc cagattgtcc gcaactacaa cgcctacctt
     5221 cgggccagcg acgatctgcc taagatgttc atcgagtccg accctgggtt cttttccaac
     5281 gctattgtcg agggagctaa gaagttccct aacaccgagt tcgtgaaggt gaagggcctc
     5341 cacttcagcc aggaggacgc tccagatgaa atgggtaagt acatcaagag cttcgtggag
     5401 cgcgtgctga agaacgagca gtaagtcgac agatcctacc tgcaaagcac cagaggatat
     5461 ggcctcggtg cctcagcatc ttcagatgtg ctgtcactgt ccaatcttca agccagcaga
     5521 gcatatgggc tcgctggctc cactgtcatt acagtggacc tgatcttctc caatcaaagg
     5581 agcagaggat atggcctcgc tccctccgac tacatcaggc tggaagtgac atccatcatt
     5641 catcccagca gagcatatgg gctcgctggg accacagcat gcctcgaagt gctgagagac
     5701 tatcatcaaa ccgacagagg atatggcctc gtcggctcct actccctcct ggtggaatac
     5761 ttctccactc ttcaacccag cagagcatat gggctcgctg ggtccatcag agatacaaga
     5821 tctctcgaca gatcctacct gcaaagcacc agaggatatg gcctcggtgc ctcagcatct
     5881 tcagatgtgc tgtcactgtc caatcttcaa gccagcagag catatgggct cgctggctcc
     5941 actgtcatta cagtggacct gatcttctcc aatcaaagga gcagaggata tggcctcgct
     6001 ccctccgact acatcaggct ggaagtgaca tccatcattc atcccagcag agcatatggg
     6061 ctcgctggga ccacagcatg cctcgaagtg ctgagagact atcatcaaac cgacagagga
     6121 tatggcctcg tcggctccta ctccctcctg gtggaatact tctccactct tcaacccagc
     6181 agagcatatg ggctcgctgg gtccatcaga gatacaagat ctctcgacag atcctacctg
     6241 caaagcacca gaggatatgg cctcggtgcc tcagcatctt cagatgtgct gtcactgtcc
     6301 aatcttcaag ccagcagagc atatgggctc gctggctcca ctgtcattac agtggacctg
     6361 atcttctcca atcaaaggag cagaggatat ggcctcgctc cctccgacta catcaggctg
     6421 gaagtgacat ccatcattca tcccagcaga gcatatgggc tcgctgggac cacagcatgc
     6481 ctcgaagtgc tgagagacta tcatcaaacc gacagaggat atggcctcgt cggctcctac
     6541 tccctcctgg tggaatactt ctccactctt caacccagca gagcatatgg gctcgctggg
     6601 tccatcagag atacaagatc tctcgacaga tcctacctgc aaagcaccag aggatatggc
     6661 ctcggtgcct cagcatcttc agatgtgctg tcactgtcca atcttcaagc cagcagagca
     6721 tatgggctcg ctggctccac tgtcattaca gtggacctga tcttctccaa tcaaaggagc
     6781 agaggatatg gcctcgctcc ctccgactac atcaggctgg aagtgacatc catcattcat
     6841 cccagcagag catatgggct cgctgggacc acagcatgcc tcgaagtgct gagagactat
     6901 catcaaaccg acagaggata tggcctcgtc ggctcctact ccctcctggt ggaatacttc
     6961 tccactcttc aacccagcag agcatatggg ctcgctgggt ccatcagaga tacaagatct
     7021 ctcgagccgc ggtgcgtaag tctgttaaca gaatgctgca tacgctgagt acttcgaaat
     7081 gtcatcctct gtctgacact gaacgtaatc cagacgcgta agtcaggccg gaaaattccc
     7141 gccaccggaa gttgagtaga cggtgctgcc tgcgactcaa ccccaggagg actgggtgaa
     7201 caaagctgcg aagtgatcca tgtaagccct cagaaccgtc tcggaaagag gaccccacat
     7261 gttgtagctt caaggcccaa tgtcagacca cgccatggcg tgccactctg cggagagtgc
     7321 agtctgcgac agtgccccag gaggactggg tgaggatcct acctacaaac ggcacgagca
     7381 tcagccgtgc cgcccagcat cttcatcact gtccaatctt caagcgtgca cgatcacggc
     7441 acgctccact gtcattacag tcttatccaa tcaaactcca gcagcatcag cgctggagct
     7501 ccgactacat caggacatcc atcattcaac gctcgacgat cacgcgagcg tccacagcat
     7561 gcctcagaca ctatcatcaa acggctgcac gatcacggca gccgctccta ctgcctctac
     7621 gttatcgact cttctgcgac gagcatcagc cgtcgcaaca tcggacatac cagatcctac
     7681 ctacaaacgg cacgagcatc agccgtgccg cccagcatct tcatcactgt ccaatcttca
     7741 agcgtgcacg atcacggcac gctccactgt cattacagtc ttatccaatc aaactccagc
     7801 agcatcagcg ctggagctcc gactacatca ggacatccat cattcaacgc tcgacgatca
     7861 cgcgagcgtc cacagcatgc ctcagacact atcatcaaac ggctgcacga tcacggcagc
     7921 cgctcctact gcctctacgt tatcgactct tctgcgacga gcatcagccg tcgcaacatc
     7981 ggacatacca gatcctacct acaaacggca cgagcatcag ccgtgccgcc cagcatcttc
     8041 atcactgtcc aatcttcaag cgtgcacgat cacggcacgc tccactgtca ttacagtctt
     8101 atccaatcaa actccagcag catcagcgct ggagctccga ctacatcagg acatccatca
     8161 ttcaacgctc gacgatcacg cgagcgtcca cagcatgcct cagacactat catcaaacgg
     8221 ctgcacgatc acggcagccg ctcctactgc ctctacgtta tcgactcttc tgcgacgagc
     8281 atcagccgtc gcaacatcgg acataccaga tcctacctac aaacggcacg agcatcagcc
     8341 gtgccgccca gcatcttcat cactgtccaa tcttcaagcg tgcacgatca cggcacgctc
     8401 cactgtcatt acagtcttat ccaatcaaac tccagcagca tcagcgctgg agctccgact
     8461 acatcaggac atccatcatt caacgctcga cgatcacgcg agcgtccaca gcatgcctca
     8521 gacactatca tcaaacggct gcacgatcac ggcagccgct cctactgcct ctacgttatc
     8581 gactcttctg cgacgagcat cagccgtcgc aacatcggac ataccagatc ttcaatcgat
     8641 ttcgcgcggg atccagacca cctcccctgc gagctaagct ggacagccaa tgacgggtaa
     8701 gagagtgaca tttttcacta acctaagaca ggagggccgt cagagctact gcctaatcca
     8761 aagacgggta aaagtgataa aaatgtatca ctccaaccta agacaggcgc agcttccgag
     8821 ggatttgaga tccagacatg ataagataca ttgatgagtt tggacaaacc aaaactagaa
     8881 tgcagtgaaa aaaatgcctt atttgtgaaa tttgtgatgc tattgcctta tttgtaacca
     8941 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     9001 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
     9061 attatgatct gaagttccta tactatttga agaataggaa cttcggaata ggaacttcgg
     9121 atctcatatg gccatatggg ggcgcctaga gaaggagtga gggctggata aagggaggat
     9181 cgaggcgggg tcgaacgagg aggttcaagg gggagagacg gggcggatgg aggaagagga
     9241 ggcggaggct tagggtgtac aaagggcttg acccagggag gggggtcaaa agccaaggct
     9301 tcccaggtca cgatgtaggg gacctggtct gggtgtccat gcgggccagg tgaaaagacc
     9361 ttgatcttaa cctgggtgat gaggtctcgg ttaaaggtgc cgtctcgcgg ccatccgacg
     9421 ttaaaggttg gccattctgc agagcagaag gtaacccaac gtctcttctt gacatctacc
     9481 gactggttgt gagcgatccg ctcgacatct ttccagtgac ctaaggtcaa acttaaggga
     9541 gtggtaacag gctagcccgg gcccatattt tcagacaaat acagaaacac agtcagacag
     9601 agacaacaca gaacgatgct gcagcagaca agacgcgcgg cgcggcttcg gtcccaaacc
     9661 gaaagcaaaa attcagacgg aggcgggaac tgttttaggt tctcgtctcc taccagaacc
     9721 acatatccct cctctaaggg gggtgcacca aagagtccta aacgatcggg attttaggac
     9781 tcaggtcggg ccacaaaaac ggcccccgaa gtccctggga cgtctcccag ggttgcggcc
     9841 gggtgttccg aactcgtcag ttccaccacg ggtccgccag atacagagct agttagctaa
     9901 ctagtaccga cgcaggcgca taaaatcagt catagacact agacaatcgg acagacacag
     9961 ataagttgct ggccagctta cctcccggtg gtgggtcggt ggtccctggg caggggtctc
    10021 ccgatcccgg acgagccccc aaatgaaaga cccccgctga cgggtagtca atcactcaga
    10081 ggagaccctc ccaaggaaca gcgagaccac aagtcggatg caactgcaag agggtttatt
    10141 ggatacacgg gtacccgggc gactcagtca atcggaggac tggcgcgccg agtgaggggt
    10201 tgtgggctct tttattgagc tcggggagca gaagcgcgcg aacagaagcg agaagcgaac
    10261 tgattggtta gttcaaataa ggcacagggt catttcaggt ccttggggca ccctggaaac
    10321 atctgatggt tctctagaaa ctgctgaggg ctggaccgca tctggggacc atctgttctt
    10381 ggccctgagc cggggcagga actgcttacc acagatatcc tgtttggccc atattcagct
    10441 gttccatctg ttcttggccc tgagccgggg caggaactgc ttaccacaga tatcctgttt
    10501 ggcccatatt cagctgttcc atctgttcct gaccttgatc tgaacttttc tattctcagt
    10561 tatgtatttt tccatgcctt gcaaaatggc gttacttaag ctagcttgcc aaacctacag
    10621 gtggggtctt tcacatgtat atgtcaaaaa taaaaatcaa ctaattgact agtaattaat
    10681 atgactggca taatgggaaa ttgatcctga cagatgcaaa ctggcttctc agcagcgcat
    10741 ttatgttgtc aactgaggaa ggaaacgtta atgacagaaa ctctaagtaa tttccacgtt
    10801 tatctatttt tatttatact agctttggta acaggaatat tgcagcattc atgcacattg
    10861 aaacccttat gaaataaaaa catctgtgca tttaaaatgg aattaacatt ttaaatgtta
    10921 aaaaaagctg gcttagcttc cccccgcccc ctagggcata gaacaagtca aatgctttat
    10981 atatttgagt ttgggatgta ttaggaaact cctaagagca aagctagctt g
//
