LOCUS       sequence-201112-        1149 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses a soma-targeted GtACR2 in Cre-expressing neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Mahn M, Gibor L, Patil P, Cohen-Kashi Malina K, Oring S, Printz Y, 
            Levy R, Lampl I, Yizhar O
  TITLE     High-efficiency optogenetic silencing with soma-targeted 
            anion-conducting channelrhodopsins.
  JOURNAL   Nat Commun. 2018 Oct 8;9(1):4125. doi: 10.1038/s41467-018-06511-8.
  PUBMED    30297821
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-10-8"; volume: "9(1):4125. doi"; pages: " 
            10.1038/s41467-018-06511-8"
COMMENT     Sequence Label: pAAV_hSyn1-SIO-stGtACR2-FusionRed
FEATURES             Location/Qualifiers
     source          1..1149
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(51..84)
                     /label=lox66
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      91..100
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     gap             1136..1148
                     /estimated_length=13
ORIGIN
        1 cnnnnnnntt nnnnnatttt gtaatccaga ggttgattat cgataagctt ataacttcgt
       61 atagcataca ttatacgaac ggtaggatcc gccaccatgg catcacaggt cgtctatggg
      121 gaatgggcat caactcacac cgagtgctac aatatgtcca gaatcgactc caccttcgtc
      181 tcactgctgc agctggtgtg ggccgtggtc tcaggctgcc agacaatctt catgattagc
      241 agggctccca aggtcccttg ggagtccgtg tacctgccct ttgtcgaatc tatcacttat
      301 gcactggcca gtactggcaa cggaaccctg cagatgaggg acggcagatt ctttccttgg
      361 agccgaatgg cctcctggct gtgcacctgt ccaatcatgc tgggacagat ttctaacatg
      421 gctctggtga agtacaaaag tatcccactg aatcccattg cacaggccgc ttccatcatt
      481 agagtggtca tgggaatcac cgctacaatt tctcccgcag agtatatgaa gtggctgttc
      541 tttttctttg gggccacctg cctggtgttc gagtacagtg tggtcttcac aatctttcaa
      601 gtggggctgt atggctttga atctgtcggg acacctctgg cacagaaggt ggtggtgagg
      661 atcaagatgc tgcgcctgat cttctttatt gcttggacta tgttcccaat cgtgtggctg
      721 atttcaccca ccggagtctg cgtgatccac gagaacgtga gcgccattct gtacctgctg
      781 gctgacggcc tgtgcaaaaa tacatatgga gtgatcctgt ggagcactgc ctggggagtg
      841 ctggaaggga agtgggnccc cgcatgtctg ccagggcagg agaaacccga agccgacgat
      901 ccttttggcc tgaatnacga naagaatgcc cctnctaacg acganntcan natcagaang
      961 nttnnnnggn ccnnnannan cngnatcnnn nncgagggnn annacatccc ccnngnnnnn
     1021 nncgacatcn nnnnngngan gnnnncnanc tgannnnnna nnnnnnncnn tnnnntntnc
     1081 ntngnnggnn ncnnnnnnnn nccnnnnnnn gcnnannnnn gnnnnnnnan ncctannnnn
     1141 nnnnnnnnc
//
