LOCUS       sequence-201228-        9112 bp    DNA     circular SYN 14-APR-2025
DEFINITION  synthetic promoter Elk1 drives mKate2 experssion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9112)
  AUTHORS   Nissim L, Wu MR, Pery E, Binder-Nissim A, Suzuki HI, Stupp D, 
            Wehrspaun C, Tabach Y, Sharp PA, Lu TK
  TITLE     Synthetic RNA-Based Immunomodulatory Gene Circuits for Cancer 
            Immunotherapy.
  JOURNAL   Cell. 2017 Nov 16;171(5):1138-1150.e15. doi: 
            10.1016/j.cell.2017.09.049. Epub 2017 Oct 19.
  PUBMED    29056342
REFERENCE   2  (bases 1 to 9112)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.09.049"; journalName: "Cell"; date: 
            "2017-11-16- 16"; volume: "171"; issue: "5"; pages: "1138-1150.e15"
COMMENT     Sequence Label:  pLN352 (FuGW-S(Elk1)p-mKate2)
FEATURES             Location/Qualifiers
     source          1..9112
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     102..118
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     103..119
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    122..710
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             228..239
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     637..657
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     regulatory      1138..1147
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1144..1857
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     misc_feature    1957..2074
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2372..2416)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2601..2834
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3327..3452
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             3499..3679
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(3697..3895)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3722..3742)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3897..4276
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     4448..4467
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        4542..4646
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4647..4715,4716..5507)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4865..4884)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5678..6266
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6167..6186
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6420..6437
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6554..6575
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6590..6595,6596..6613,6614..6620)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6628..6644
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6633..6655
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6652..6668
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6652..6668
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    6717..6838
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6728..6747)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6782..6801
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(6968..7342)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(7361..7408)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(7456..7785)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7470..7605
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7524..7543)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     7770..7790
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(7799..8227)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7909..7930)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8121..8140
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    8273..8497
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     8486..8503
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             8529..8709
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ggaaaaccca cctcctcctc ctcttgtgct tctagccagg cacaatcagc attggtagct
       61 gctgtattgc tacttgtgat tgctccatgt ttttctaggt ctcgaggtcg acggtatcga
      121 tgcggggagg cggcccaaag ggagatccga ctcgtctgag ggcgaaggcg aagacgcgga
      181 agaggccgca gagccggcag caggccgcgg gaaggaaggt ccgctggatt gagggccgaa
      241 gggacgtagc agaaggacgt cccgcgcaga atccaggtgg caacacaggc gagcagccaa
      301 ggaaaggacg atgatttccc cgacaacacc acggaattgt cagtgcccaa cagccgagcc
      361 cctgtccagc agcgggcaag gcaggcggcg atgagttccg ccgtggcaat agggaggggg
      421 aaagcgaaag tcccggaaag gagctgacag gtggtggcaa tgccccaacc agtgggggtt
      481 gcgtcagcaa acacagtgca caccacgcca cgttgcctga caacgggcca caactcctca
      541 taaagagaca gcaaccagga tttatacaag gaggagaaaa tgaaagccat acgggaagca
      601 atagcatgat acaaaggcat taaagcagcg tatccacata gcgtaaaagg agcaacatag
      661 ttaagaatac cagtcaatct ttcacaaatt ttgtaatcca gaggttgatt atcgataagc
      721 ttgatatcgc ggccgccctg cacctgcagg aaacaggaag ttcgtagccc aaccggaagt
      781 atcaaccgga agtagacgac cggaagtaac tgaccggaag tagtcgaccg gaagtggaga
      841 aacaggaagt tcgttcccca accggaagtc gaattcctgc cctactgaca ctgcctgccg
      901 gaccggaagt aagcaaccgg aagtaatcga ccggaagtgg acaaacagga agttcgtact
      961 ccaaccggaa gtgtcaaccg gaagtagagg accggaagta tccgaccgga agtgcgaata
     1021 tccgcggcgc gccagacgct agcggggggc tataaaaggg ggtgggggcg ttcgtcctca
     1081 ctctagatct gcgatctaag taagcttggc attccggtac tgttggtaaa ccagctcgcc
     1141 accatggtgt ctaagggcga agagctgatt aaggagaaca tgcacatgaa gctgtacatg
     1201 gagggcaccg tgaacaacca ccacttcaag tgcacatccg agggcgaagg caagccctac
     1261 gagggcaccc agaccatgag aatcaaggtg gtcgagggcg gccctctccc cttcgccttc
     1321 gacatcctgg ctaccagctt catgtacggc agcaaaacct tcatcaacca cacccagggc
     1381 atccccgact tctttaagca gtccttccct gagggcttca catgggagag agtcaccaca
     1441 tacgaagacg ggggcgtgct gaccgctacc caggacacca gcctccagga cggctgcctc
     1501 atctacaacg tcaagatcag aggggtgaac ttcccatcca acggccctgt gatgcagaag
     1561 aaaacactcg gctgggaggc ctccaccgag atgctgtacc ccgctgacgg cggcctggaa
     1621 ggcagaagcg acatggccct gaagctcgtg ggcgggggcc acctgatctg caacttgaag
     1681 accacataca gatccaagaa acccgctaag aacctcaaga tgcccggcgt ctactatgtg
     1741 gacagaagac tggaaagaat caaggaggcc gacaaagaga cctacgtcga gcagcacgag
     1801 gtggctgtgg ccagatactg cgacctccct agcaaactgg ggcacaaact taattgataa
     1861 accggttgca gtcccgggta agcgggactc tggggttcga aatgaccgac caagcattaa
     1921 ccaaactgga tctctgctgt ccctgtaata aacccgaaaa ttttgaattt ttgtaatttg
     1981 tttttgtaat tctttagttt gtatgtctgt tgctattatg tctactattc tttcccctgc
     2041 actgtacccc ccaatccccc cttttctttt aaaattgtgg atgaatactg ccatttgtct
     2101 gcagaattgg cgcacgcagt gccgatccgt tcactaatcg aatggatctg tctctgtctc
     2161 tctctccacc ttcttcttct attccttcgg gcctgtcggg tcccctcggg gttgggaggt
     2221 gggtctgaaa cgataatggt gaatatccct gcctaactct attcactata gaaagtacag
     2281 caaaaactat tcttaaacct accaagcctc ctactatcat tatgaataat tttatatacc
     2341 acagccaatt tgttatgtta aaccaattcc acaaacttgc ccatttatct aattccaata
     2401 attcttgttc attcttttct tgctggtttt gcgattcttc aattaaggag tgtattaagc
     2461 ttgtgtaatt gttaatttct ctgtcccact ccatccaggt cgtgtgattc caaatctgtt
     2521 ccagagattt attactccaa ctagcattcc aaggcacagc agtggtgcaa atgagttttc
     2581 cagagcaacc ccaaatcccc aggagctgtt gatcctttag gtatctttcc acagccagga
     2641 ttcttgcctg gagctgcttg atgccccaga ctgtgagttg caacagatgc tgttgcgcct
     2701 caatagccct cagcaaattg ttctgctgct gcactatacc agacaataat tgtctggcct
     2761 gtaccgtcag cgtcattgac gctgcgccca tagtgcttcc tgctgctccc aagaacccaa
     2821 ggaacaaagc tcctattccc actgctcttt tttctctctg caccactctt ctctttgcct
     2881 tggtgggtgc tactcctaat ggttcaattt ttactacttt atatttatat aattcacttc
     2941 tccaattgtc cctcatatct cctcctccag gtctgaagat cagcggccgc ttgctgtgcg
     3001 gtggtcttac ttttgttttg ctcttcctct atcttgtcta aagcttcctt ggtgtctttt
     3061 atctctatcc tttgatgcac acaatagagg gttgctactg tattatataa tgatctaagt
     3121 tcttctgatc ctgtctgaag ggatggttgt agctgtccca gtatttgtct acagccttct
     3181 gatgtttcta acaggccagg attaactgcg aatcgttcta gctccctgct tgcccatact
     3241 atatgtttta atttatattt tttctttccc cctggcctta accgaatttt ttcccatcgc
     3301 gatctaattc tcccccgctt aatactgacg ctctcgcacc catctctctc cttctagcct
     3361 ccgctagtca aaatttttgg cgtactcacc agtcgccgcc cctcgcctct tgccgtgcgc
     3421 gcttcagcaa gccgagtcct gcgtcgagag agctcctctg gtttcccttt cgctttcaag
     3481 tccctgttcg ggcgccactg ctagagattt tccacactga ctaaaagggt ctgagggatc
     3541 tctagttacc agagtcacac aacagacggg cacacactac ttgaagcact caaggcaagc
     3601 tttattgagg cttaagcagt gggttcccta gttagccaga gagctcccag gctcagatct
     3661 ggtctaacca gagagaccca gtacaggcaa aacgcgctgc ttatatagac ctcccaccgt
     3721 acacgcctac cgcccatttg cgtcaatggg gcggagttgt tacgacattt tggaaagtcc
     3781 cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa tggggtggag acttggaaat
     3841 ccccgtgagt caaaccgcta tccacgccca ttgatgtact gccaaaaccg catcaccatg
     3901 gtaatagcga tgactaatac gtagatgtac tgccaagtag gaaagtccca taaggtcatg
     3961 tactgggcat aatgccaggc gggccattta ccgtcattga cgtcaatagg gggcgtactt
     4021 ggcatatgat acacttgatg tactgccaag tgggcagttt accgtaaata ctccacccat
     4081 tgacgtcaat ggaaagtccc tattggcgtt actatgggaa catacgtcat tattgacgtc
     4141 aatgggcggg ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg
     4201 cggaactcca tatatgggct atgaactaat gaccccgtaa ttgattacta ttaataacta
     4261 gtcaataatc aatgtcaacg cgtatatctg gcccgtacat cgcgaagcag cgcaaaacgc
     4321 ctaaccctaa gcagattctt catgcaattg tcggtcaagc cttgccttgt tgtagcttaa
     4381 attttgctcg cgcactactc agcgacctcc aacacacaag cagggagcag atactggctt
     4441 aactatgcgg catcagagca gattgtactg agagtgcacc ataggggatc gggagatctc
     4501 ccgatccgtc gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     4561 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     4621 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     4681 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     4741 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     4801 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     4861 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     4921 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     4981 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     5041 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     5101 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     5161 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     5221 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     5281 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     5341 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     5401 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     5461 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     5521 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     5581 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     5641 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     5701 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     5761 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     5821 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     5881 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     5941 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     6001 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     6061 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     6121 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     6181 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     6241 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     6301 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     6361 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     6421 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     6481 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     6541 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     6601 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     6661 gctatgacca tgattacgcc aagctctagc tagaggtcga cggtatacag acatgataag
     6721 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     6781 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttgg
     6841 ggtgggcgaa gaactccagc atgagatccc cgcgctggag gatcatccag ccggcgtccc
     6901 ggaaaacgat tccgaagccc aacctttcat agaaggcggc ggtggaatcg aaatctcgta
     6961 gcacgtgtca gtcctgctcc tcggccacga agtgcacgca gttgccggcc gggtcgcgca
     7021 gggcgaactc ccgcccccac ggctgctcgc cgatctcggt catggccggc ccggaggcgt
     7081 cccggaagtt cgtggacacg acctccgacc actcggcgta cagctcgtcc aggccgcgca
     7141 cccacaccca ggccagggtg ttgtccggca ccacctggtc ctggaccgcg ctgatgaaca
     7201 gggtcacgtc gtcccggacc acaccggcga agtcgtcctc cacgaagtcc cgggagaacc
     7261 cgagccggtc ggtccagaac tcgaccgctc cggcgacgtc gcgcgcggtg agcaccggaa
     7321 cggcactggt caacttggcc atggtttagt tcctcacctt gtcgtattat actatgccga
     7381 tatactatgc cgatgattaa ttgtcaacac gtgctgatca gatccgaaaa tggatataca
     7441 agctcccggg agctttttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg
     7501 gaatagctca gaggcagagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc
     7561 atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg
     7621 cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga
     7681 gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct
     7741 gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc acagaattaa
     7801 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     7861 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     7921 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     7981 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     8041 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     8101 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     8161 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     8221 ggcgcgtggg gataccccct agagccccag ctggttcttt ccgcctcaga agccatagag
     8281 cccaccgcat ccccagcatg cctgctattg tcttcccaat cctccccctt gctgtcctgc
     8341 cccaccccac cccccagaat agaatgacac ctactcagac aatgcgatgc aatttcctca
     8401 ttttattagg aaaggacagt gggagtggca ccttccaggg tcaaggaagg cacgggggag
     8461 gggcaaacaa cagatggctg gcaactagaa ggcacagtcg aggctgatca gcgggtttaa
     8521 acgggccctg ctagagattt tccacactga ctaaaagggt ctgagggatc tctagttacc
     8581 agagtcacac aacagacggg cacacactac ttgaagcact caaggcaagc tttattgagg
     8641 cttaagcagt gggttcccta gttagccaga gagctcccag gctcagatct ggtctaacca
     8701 gagagaccca gtacagtccg gatgcagctc tcgggccatg tgatgaaatg ctaggcggct
     8761 gtcaaacctc cactctaata cttctctctc cgggtcatcc atcccatgca ggctcacagg
     8821 gtgtaacaag cgggtgttct ctccttcatt ggcttcttct accttctctt gctcaactgg
     8881 tactagcttg tagcaccatc caaaggtcag tggatatctg atccctggcc ctggtgtgta
     8941 gttctgccaa tcagggaagt agccttgtgt gtggtagatc cacagatcaa ggatatcttg
     9001 tcttcgttgg gagtgaatta gcccttccag tccccccttt tcttttaaaa agtggctaag
     9061 atctacagct gccttgtaag tcattggtct taaaggtacc tgaggtgtga ct
//
