LOCUS       sequence-201264-        9889 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Module 3 - GAL4 synthetic promoter (with eight binding sites) drives
            the expression of IL12 transcripts containing miR1-Bs(Pe).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9889)
  AUTHORS   Nissim L, Wu MR, Pery E, Binder-Nissim A, Suzuki HI, Stupp D, 
            Wehrspaun C, Tabach Y, Sharp PA, Lu TK
  TITLE     Synthetic RNA-Based Immunomodulatory Gene Circuits for Cancer 
            Immunotherapy.
  JOURNAL   Cell. 2017 Nov 16;171(5):1138-1150.e15. doi: 
            10.1016/j.cell.2017.09.049. Epub 2017 Oct 19.
  PUBMED    29056342
REFERENCE   2  (bases 1 to 9889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.09.049"; journalName: "Cell"; date: 
            "2017-11-16- 16"; volume: "171"; issue: "5"; pages: "1138-1150.e15"
COMMENT     Sequence Label: pLN481 (FuGW-G8p-IL12-1Pe)
FEATURES             Location/Qualifiers
     source          1..9889
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    241..358
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    2382..2970
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2435..2455)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2853..2864)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2973..2989)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2974..2990)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3495..3675
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3701..3718)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3707..3931
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3977..4405
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4064..4083)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4274..4295
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4414..4434)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4419..4748
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4599..4734
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4661..4680
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4796..4843
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4862..5236
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5366..5487
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5403..5422)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5457..5476
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5536..5552)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5536..5552)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5549..5571)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5560..5576
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5584..5590,5591..5608,5609..5614))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5629..5650
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5767..5784)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5938..6526)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6018..6037)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6697..7488,7489..7557))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7320..7339
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7558..7662)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7737..7756)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7928..8307
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8309..8507
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8462..8482
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8525..8705
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8752..8877
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9370..9603
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9788..9832
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
       61 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
      121 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
      181 gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa atggcagtat tcatccacaa
      241 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
      301 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
      361 ggtttattac agggacagca gagatccagt ttggttaatc ctgcaggcga taggtaccga
      421 gtttctagac ggagtactgt cctccgagcg gagtactgtc ctccgactcg agcggagtac
      481 tgtcctccga tcggagtact gtcctccgcg aattccggag tactgtcctc cgaagacgct
      541 agacggagta ctgtcctccg agcggagtac tgtcctccga ctcgagcgga gtactgtcct
      601 ccggctagcg gggggctata aaagggggtg ggggcgttcg tcctcactct agatctgcga
      661 tctaagtaag cttggcattc cggtactggg cgcgccacca tgtgtcacca acagttggtt
      721 atctcttggt tcagtcttgt cttcctggct tcccccctgg tagccatctg ggagttgaag
      781 aaagatgtgt acgtggtgga attggactgg tatccggacg cacccggtga gatggtggta
      841 cttacctgcg acacccccga ggaggacgga atcacctgga ctcttgatca gtctagcgag
      901 gtcttgggta gtggaaagac cctgaccatt caggtgaagg aattcggaga tgcagggcag
      961 tacacatgtc acaagggtgg ggaagtgctc tctcattccc ttcttctcct ccacaaaaag
     1021 gaggacggaa tttggtcaac cgatattctg aaggatcaga aagagccaaa aaacaaaaca
     1081 ttcctgaggt gtgaagccaa gaactacagc ggacggttta cgtgttggtg gctgactaca
     1141 atttcaaccg atctgacttt cagtgtgaag tcctcccgcg gcagctcaga tccccagggg
     1201 gtgacatgtg gcgcagcgac gctctccgca gagcgagtga ggggggataa caaagaatac
     1261 gaatatagcg tggagtgcca ggaggattcc gcctgcccag ccgccgaaga gtccctgccc
     1321 atcgaggtta tggtcgacgc agtgcataaa ctcaaatacg agaactatac gtcctccttt
     1381 ttcatacgcg acatcatcaa acctgacccc ccaaaaaacc ttcaactcaa acccctgaaa
     1441 aatagtcggc aggtggaggt cagttgggaa tacccggata cttggagtac tccgcactcc
     1501 tatttttcct tgactttttg tgtccaggta cagggaaaga gcaaaaggga aaagaaggac
     1561 cgcgtcttca ctgacaagac atcagctaca gtgatctgtc gaaagaatgc aagcattagc
     1621 gtgagagccc aggacaggta ctatagttct agctggtcag aatgggcaag cgtaccttgc
     1681 tctggcggtg gaggctccgg cggggggggg agcggcgggg gaggatctcg gaatctccct
     1741 gttgccactc cagacccagg tatgtttcca tgcctgcacc actctcagaa cttgctccgg
     1801 gctgtctcta acatgctgca gaaggccagg cagacattgg agttttaccc ctgtacgtct
     1861 gaagagatcg atcatgagga cattacaaag gataaaacat caacggtcga agcctgcctt
     1921 ccccttgagc ttaccaaaaa tgaaagctgt ctgaatagcc gcgaaacatc ctttattact
     1981 aatggttcat gcctcgcctc caggaaaaca tcttttatga tggcgctctg tctcagttct
     2041 atttatgagg atctcaaaat gtatcaagtt gagttcaaga ccatgaacgc caaactgctg
     2101 atggatccta aacgccagat ctttttggat caaaatatgc ttgctgtcat cgacgaactc
     2161 atgcaggccc tgaatttcaa ctccgagacc gttccccaaa aatcatccct ggaagagccc
     2221 gacttttaca aaacaaagat caagctctgc attcttctgc acgcttttcg gatccgagct
     2281 gtgactatag acagagtgat gtcctacctg aatgctagtt aaaccggtgc accgcttgaa
     2341 gtctttaatt aaatacaccg gtcgatatca agcttatcga taatcaacct ctggattaca
     2401 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     2461 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     2521 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     2581 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     2641 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     2701 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     2761 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     2821 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     2881 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     2941 gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga gacctagaaa
     3001 aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag
     3061 aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa
     3121 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     3181 ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag
     3241 gctacttccc tgattggcag aactacacac cagggccagg gatcagatat ccactgacct
     3301 ttggatggtg ctacaagcta gtaccagttg agcaagagaa ggtagaagaa gccaatgaag
     3361 gagagaacac ccgcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
     3421 aagtattaga gtggaggttt gacagccgcc tagcatttca tcacatggcc cgagagctgc
     3481 atccggactg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     3541 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     3601 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     3661 ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt
     3721 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     3781 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     3841 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     3901 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     3961 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     4021 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     4081 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     4141 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     4201 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     4261 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     4321 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     4381 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
     4441 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     4501 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     4561 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     4621 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     4681 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     4741 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga
     4801 caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac
     4861 catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt
     4921 cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg
     4981 tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga
     5041 caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga
     5101 ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca
     5161 gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc
     5221 cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct tctatgaaag
     5281 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     5341 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     5401 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     5461 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
     5521 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     5581 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     5641 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     5701 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     5761 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     5821 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     5881 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     5941 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     6001 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     6061 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     6121 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     6181 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     6241 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     6301 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     6361 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     6421 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     6481 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     6541 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     6601 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     6661 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     6721 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     6781 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     6841 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     6901 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     6961 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     7021 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     7081 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     7141 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     7201 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     7261 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     7321 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     7381 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     7441 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     7501 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     7561 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     7621 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     7681 gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca
     7741 atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc
     7801 gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc
     7861 atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat
     7921 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
     7981 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     8041 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     8101 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     8161 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     8221 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     8281 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
     8341 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
     8401 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
     8461 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg
     8521 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     8581 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     8641 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     8701 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
     8761 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
     8821 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
     8881 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
     8941 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
     9001 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
     9061 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
     9121 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
     9181 gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg
     9241 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     9301 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     9361 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     9421 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     9481 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     9541 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     9601 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     9661 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     9721 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     9781 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     9841 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgata
//
