LOCUS       sequence-201294-       10648 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses HyPer2 in plant cells-biosensor for Hydrogen peroxide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10648)
  AUTHORS   Exposito-Rodriguez M, Laissue PP, Yvon-Durocher G, Smirnoff N, 
            Mullineaux PM
  TITLE     Photosynthesis-dependent H2O2 transfer from chloroplasts to nuclei 
            provides a high-light signalling mechanism.
  JOURNAL   Nat Commun. 2017 Jun 29;8(1):49. doi: 10.1038/s41467-017-00074-w.
  PUBMED    28663550
REFERENCE   2  (bases 1 to 10648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-06-29"; volume: "8(1):49. doi"; pages: " 
            10.1038/s41467-017-00074-w"
COMMENT     Sequence Label: pUB-cHyPer2
FEATURES             Location/Qualifiers
     source          1..10648
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1334..1963
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2392..3465
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3531..3725
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(3961..3983)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4069..4209
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4124..4143
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4224..4241)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4395..4983)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4475..4494)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5229..6020)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    6541..6565
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(6658..6910)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(6930..7481)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(7523..7702)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     protein_bind    8124..8148
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      9607..9616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(9633..9657)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(10348..10364)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10348..10364)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10361..10383)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10372..10388
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10396..10402,10403..10420,10421..10426))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10441..10462
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    join(10627..10648,1..3)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc gtgaaaaggt
       61 ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttcccctcg ggatcaaagt
      121 actttaaagt actttaaagt actttaaagt actttgatcc aacccctccg ctgctatagt
      181 gcagtcggct tctgacgttc agtgcagccg tcttctgaaa acgacatgtc gcacaagtcc
      241 taagttacgc gacaggctgc cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt
      301 agtcgcataa agtagaatac ttgcgactag aaccggagac attacgccat gaacaagagc
      361 gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg acgaccagga cttgaccaac
      421 caacgggccg aactgcacgc ggccggctgc accaagctgt tttccgagaa gatcaccggc
      481 accaggcgcg accgcccgga gctggccagg atgcttgacc acctacgccc tggcgacgtt
      541 gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc gcgacctact ggacattgcc
      601 gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc
      661 accacgccgg ccggccgcat ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt
      721 tccctaatca tcgaccgcac ccggagcggg cgcgaggccg ccaaggcccg aggcgtgaag
      781 tttggccccc gccctaccct caccccggca cagatcgcgc acgcccgcga gctgatcgac
      841 caggaaggcc gcaccgtgaa agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg
      901 taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc
      961 ttccgtgagg acgcattgac cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa
     1021 gaggaacaag catgaaaccg caccaggacg gccaggacga accgtttttc attaccgaag
     1081 agatcgaggc ggagatgatc gcggccgggt acgtgttcga gccgcccgcg cacgtctcaa
     1141 ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc caagctggcg gcctggccgg
     1201 ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt
     1261 aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac
     1321 gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt caggcaagac
     1381 gaccatcgca acccatctag cccgcgccct gcaactcgcc ggggccgatg ttctgttagt
     1441 cgattccgat ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct
     1501 aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg
     1561 cgacttcgta gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa
     1621 ggcagccgac ttcgtgctga ttccggtgca gccaagccct tacgacatat gggccaccgc
     1681 cgacctggtg gagctggtta agcagcgcat tgaggtcacg gatggaaggc tacaagcggc
     1741 ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct
     1801 ggccgggtac gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg
     1861 cactgccgcc gccggcacaa ccgttcttga atcagaaccc gagggcgacg ctgcccgcga
     1921 ggtccaggcg ctggccgctg aaattaaatc aaaactcatt tgagttaatg aggtaaagag
     1981 aaaatgagca aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg cagcagcaag
     2041 gctgcaacgt tggccagcct ggcagacacg ccagccatga agcgggtcaa ctttcagttg
     2101 ccggcggagg atcacaccaa gctgaagatg tacgcggtac gccaaggcaa gaccattacc
     2161 gagctgctat ctgaatacat cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag
     2221 tagatgaatt ttagcggcta aaggaggcgg catggaaaat caagaacaac caggcaccga
     2281 cgccgtggaa tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt
     2341 tgtctgccgg ccctgcaatg gcactggaac ccccaagccc gaggaatcgg cgtgagcggt
     2401 cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg gtgatgacct ggtggagaag
     2461 ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa
     2521 tcgtggcaag cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt
     2581 gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac cagatttttt cgttccgatg
     2641 ctctatgacg tgggcacccg cgatagtcgc agcatcatgg acgtggccgt tttccgtctg
     2701 tcgaagcgtg accgacgagc tggcgaggtg atccgctacg agcttccaga cgggcacgta
     2761 gaggtttccg cagggccggc cggcatggcc agtgtgtggg attacgacct ggtactgatg
     2821 gcggtttccc atctaaccga atccatgaac cgataccggg aagggaaggg agacaagccc
     2881 ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt tctgccggcg agccgatggc
     2941 ggaaagcaga aagacgacct ggtagaaacc tgcattcggt taaacaccac gcacgttgcc
     3001 atgcagcgta cgaagaaggc caagaacggc cgcctggtga cggtatccga gggtgaagcc
     3061 ttgattagcc gctacaagat cgtaaagagc gaaaccgggc ggccggagta catcgagatc
     3121 gagctagctg attggatgta ccgcgagatc acagaaggca agaacccgga cgtgctgacg
     3181 gttcaccccg attacttttt gatcgatccc ggcatcggcc gttttctcta ccgcctggca
     3241 cgccgcgccg caggcaaggc agaagccaga tggttgttca agacgatcta cgaacgcagt
     3301 ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat
     3361 gacctgccgg agtacgattt gaaggaggag gcggggcagg ctggcccgat cctagtcatg
     3421 cgctaccgca acctgatcga gggcgaagca tccgccggtt cctaatgtac ggagcagatg
     3481 ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag gtctctttcc tgtggatagc
     3541 acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat tgggaaccca
     3601 aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga gaaaaaaggc
     3661 gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg cctggcctgt
     3721 gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac ccttcggtcg
     3781 ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa
     3841 atggctggcc tacggccagg caatctacca gggcgcggac aagccgcgcc gtcgccactc
     3901 gaccgccggc gcccacatca aggcaccctg cctcgcgcgt ttcggtgatg acggtgaaaa
     3961 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     4021 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac
     4081 ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt
     4141 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     4201 cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     4261 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     4321 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     4381 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     4441 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     4501 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     4561 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     4621 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     4681 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     4741 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     4801 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     4861 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     4921 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     4981 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     5041 taagggattt tggtcatgca tgatatatct cccaatttgt gtagggctta ttatgcacgc
     5101 ttaaaaataa taaaagcaga cttgacctga tagtttggct gtgagcaatt atgtgcttag
     5161 tgcatctaac gcttgagtta agccgcgccg cgaagcggcg tcggcttgaa cgaatttcta
     5221 gctagacatt atttgccgac taccttggtg atctcgcctt tcacgtagtg gacaaattct
     5281 tccaactgat ctgcgcgcga ggccaagcga tcttcttctt gtccaagata agcctgtcta
     5341 gcttcaagta tgacgggctg atactgggcc ggcaggcgct ccattgccca gtcggcagcg
     5401 acatccttcg gcgcgatttt gccggttact gcgctgtacc aaatgcggga caacgtaagc
     5461 actacatttc gctcatcgcc agcccagtcg ggcggcgagt tccatagcgt taaggtttca
     5521 tttagcgcct caaatagatc ctgttcagga accggatcaa agagttcctc cgccgctgga
     5581 cctaccaagg caacgctatg ttctcttgct tttgtcagca agatagccag atcaatgtcg
     5641 atcgtggctg gctcgaagat acctgcaaga atgtcattgc gctgccattc tccaaattgc
     5701 agttcgcgct tagctggata acgccacgga atgatgtcgt cgtgcacaac aatggtgact
     5761 tctacagcgc ggagaatctc gctctctcca ggggaagccg aagtttccaa aaggtcgttg
     5821 atcaaagctc gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag caaatcaata
     5881 tcactgtgtg gcttcaggcc gccatccact gcggagccgt acaaatgtac ggccagcaac
     5941 gtcggttcga gatggcgctc gatgacgcca actacctctg atagttgagt cgatacttcg
     6001 gcgatcaccg cttcccccat gatgtttaac tttgttttag ggcgactgcc ctgctgcgta
     6061 acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg
     6121 gatgcccgag gcatagactg taccccaaaa aaacagtcat aacaagccat gaaaaccgcc
     6181 actgcgccgt taccaccgct gcgttcggtc aaggttctgg accagttgcg tgagcgcata
     6241 cgctacttgc attacagctt acgaaccgaa caggcttatg tccactgggt tcgtgcccga
     6301 attgatcaca ggcagcaacg ctctgtcatc gttacaatca acatgctacc ctccgcgaga
     6361 tcatccgtgt ttcaaacccg gcagcttagt tgccgttctt ccgaatagca tcggtaacat
     6421 gagcaaagtc tgccgcctta caacggctct cccgctgacg ccgtcccgga ctgatgggct
     6481 gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg
     6541 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
     6601 gacgttttta atgtactgaa ttaacgccga attgaattat cagcttgcat gccggtcgat
     6661 ctagtaacat agatgacacc gcgcgcgata atttatccta gtttgcgcgc tatattttgt
     6721 tttctatcgc gtattaaatg tataattgcg ggactctaat cataaaaacc catctcataa
     6781 ataacgtcat gcattacatg ttaattatta catgcttaac gtaattcaac agaaattata
     6841 tgataatcat cgcaagaccg gcaacaggat tcaatcttaa gaaactttat tgccaaatgt
     6901 ttgaacgatc tgcttgactc taggggtcat cagatttcgg tgacgggcag gaccggacgg
     6961 ggcggcaccg gcaggctgaa gtccagctgc cagaaaccca cgtcatgcca gttcccgtgc
     7021 ttgaagccgg ccgcccgcag catgccgcgg ggggcatatc cgagcgcctc gtgcatgcgc
     7081 acgctcgggt cgttgggcag cccgatgaca gcgaccacgc tcttgaagcc ctgtgcctcc
     7141 agggacttca gcaggtgggt gtagagcgtg gagcccagtc ccgtccgctg gtggcggggg
     7201 gagacgtaca cggtcgactc ggccgtccag tcgtaggcgt tgcgtgcctt ccagggaccc
     7261 gcgtaggcga tgccggcgac ctcgccgtcc acctcggcga cgagccaggg atagcgctcc
     7321 cgcagacgga cgaggtcgtc cgtccactcc tgcggttcct gcggctcggt acggaagttg
     7381 accgtgcttg tctcgatgta gtggttgacg atggtgcaga ccgccggcat gtccgcctcg
     7441 gtggcacggc ggatgtcggc cgggcgtcgt tctgggctca tggtagatcc cctcgatcga
     7501 gttgagagtg aatatgagac tctaattgga taccgagggg aatttatgga acgtcagtgg
     7561 agcatttttg acaagaaata tttgctagct gatagtgacc ttaggcgact tttgaacgcg
     7621 caataatggt ttctgacgta tgtgcttagc tcattaaact ccagaaaccc gcggctcagt
     7681 ggctccttca acgttgcggt tctgtcagtt ccaaacgtaa aacggcttgt cccgcgtcat
     7741 cggcgggggt cataacgtga ctcccttaat tctccgctca tgatcgataa ttcgcggtac
     7801 ccggggatcc tctagagggc ccgacgtcgc atgcctgcag gtcactggat tttggtttta
     7861 ggaattagaa attttattga tagaagtatt ttacaaatac aaatacatac taagggtttc
     7921 ttatatgctc aacacatgag cgaaacccta taagaaccct aattccctta tctgggaact
     7981 actcacacat tattctggag aaaaatagag agagatagat ttgtagagag agactggtga
     8041 tttttgcgga ctctagcatg gccgcttata aactagttgg atatctcgag tcgacacgcg
     8101 tagatctccg cggacgtccc atcaccactt tgtacaagaa agctgggttc agttacctag
     8161 atccggtgga tcctcacact gcttgcttca aaaccttatc aaagtgacca tccatcctgg
     8221 ctcttatagc ctctgctaac tgttcatacc ttgatctcaa tggtgaacct ggcctgtaga
     8281 ccaatccaat ggtccttctt ggctctggtt taatgcatgg aagataaact acaccatccc
     8341 tcttcctctc tggtggcacg gcaagtgctg gtaacaaagt tattccactt ccaacggcta
     8401 ccatgttcct caaggtttct agtgatgtag ccctgaagtg agtatcctcg tcagcaccgg
     8461 cttcgaagca aaatccggtt ccgttgtact ctagcttgtg tccaagaata tttccgtcct
     8521 ctttgaatcc gataccttta agctctattc tattcaccaa agtatctccc tcaaacttca
     8581 cttctgccct agttttataa ttaccgtcgt ctttgaagaa tatagtcctc tcttgcacat
     8641 atccctcagg catagcactc ttgaagaagt catgttgctt catatggtca ggatatcttg
     8701 cgaagcactt taaaccgtaa cctaaggttg ttactaatgt tggccatggg acaggaagtt
     8761 ttccagttgt acagattaat ttcaatgtta gttttccgta ggttgcgtca ccctcacctt
     8821 ctccggagac tgaaaatttg tgaccgttca cgtcaccatc aagttctact aatattggga
     8881 caactcctgt gaacaactct tctcccttgg aacctgtacc tccactacct ccgtctacat
     8941 tgtatagctc atccatacca agagtaatac cggcagcggt tacgaactca agcaacacca
     9001 tgtgatccct tttctcattt gggtctttgg ataggacaga ctgaaaagat aggtagtggt
     9061 tatctggaag taaaacaggt ccgtcaccga ttggggtgtt ctgttgataa tggtctgcta
     9121 gttgtacgga accatcttcc acgttatgtc taattttgaa gttggccttt ataccattct
     9181 tctgtttgtc ggccatgata tagacgttat ctgaattgta accggcggac atagcttgat
     9241 ccctcaagca gtgtccatcc tcaagcatta gaagtttctc accggccaaa tctgccattg
     9301 gaacgcattc tctattggcc catgggtgat cttcatagat tgctaaaagc ataggttcat
     9361 cgaaaagagg cacttctatg aatgcctcgg attcctttac caaagctagt atgacacaat
     9421 caagtttacc actatctaac tgggccaata gttgatgagt ctgtgcctcg tgaaggtaca
     9481 tttcaagttt agggaaagtt tggtgtagca ttgggataat gtgaggaagt aagtaaggtc
     9541 caactgttgg gattagaccg atatgcaatg gtcctgacat agtctcaccc tgttgagaag
     9601 ccatttccat ggtggcgacc ggtagcgcta gcagcctgct tttttgtaca aacttgtgat
     9661 gggccctcta gaggatcccg cactcgagtg ttaatcagaa aaactcagat taatctacaa
     9721 attcgatcgc acaaactaga aactaacacc agatctagat agaaatcaca aatcgaagag
     9781 taattattcg acaaaactca aattatttga acaaatcgga tgatatctat gaaaccctaa
     9841 tcgagaatta agatgatatc taacgatcaa acccagaaaa tcgtcttcga tctaagatta
     9901 acagaatcta aaccaaagaa catatacgaa attgggatcg aacgaaaaca aaatcgaaga
     9961 ttttgagaga ataaggaaca cagaaattta ccttgatcac ggtagagaga attgagagaa
    10021 agtttttaag attttgagaa attgaaatct gaattgtgaa gaagaagagc tctttgggta
    10081 ttgttttata gaagaagaag aagaaaagac gaggacgact aggtcacgag aaagctaagg
    10141 cggtgaagca atagctaata ataaaatgac acgtgtattg agcgttgttt acacgcaaag
    10201 ttgtttttgg ctaattgcct tatttttagg ttgaggaaaa gtatttgtgc tttgagttga
    10261 taaacacgac tcgtgtgtgc cggctgcaac cactttgacg ccgtttatta ctgactcgtc
    10321 gggtaccgag ctcgaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc
    10381 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
    10441 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
    10501 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
    10561 tggctagagc agcttgagct tggatcagat tgtcgtttcc cgccttcagt ttaaactatc
    10621 agtgtttgac aggatatatt ggcgggta
//
