LOCUS       sequence-201565-       14011 bp    DNA     circular SYN 15-APR-2025
DEFINITION  lentiviral expression of Psd95 shRNA and SAP97alpha-EGFP fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14011)
  AUTHORS   Schluter OM, Xu W, Malenka RC
  TITLE     Alternative N-terminal domains of PSD-95 and SAP97 govern 
            activity-dependent regulation of synaptic AMPA receptor function.
  JOURNAL   Neuron. 2006 Jul 6;51(1):99-111.
  PUBMED    16815335
REFERENCE   2  (bases 1 to 14011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron."; 
            date: "2006-07-6"; volume: "51(1)"; pages: "99-111"
COMMENT     Sequence Label: FH95pUp97aGW (C3)
FEATURES             Location/Qualifiers
     source          1..14011
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3436..3480
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3778..3895
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3948..4162
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     4083..4102
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     promoter        4266..5477
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5314..5333
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      7941..7950
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7947..7949,7950..7952,7953..8666)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7947..7949,7950..7952,7953..8663)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7992..8013)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8253..8272)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8600..8621
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    8709..9297
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8762..8782)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9180..9191)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9816..9996
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10022..10039)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10028..10252
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10298..10726
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10385..10404)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10595..10616
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10735..10755)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10740..11069
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10920..11055
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10982..11001
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        11117..11164
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             11183..11557
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    11687..11808
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11724..11743)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11778..11797
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11857..11873)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11857..11873)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11870..11892)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11881..11897
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11905..11911,11912..11929,11930..11935))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11950..11971
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12088..12105)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12259..12847)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12339..12358)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13018..13809,13810..13878))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13641..13660
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13879..13983)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat actgagagat cccctcataa tttccccaaa gcgtaaccat
     2101 gtgtgaataa attttgagct agtagggttg cagccacgag taagtcttcc cttgttattg
     2161 tgtagccaga atgccgcaaa acttccatgc ctaagcgaac tgttgagagt acgtttcgat
     2221 ttctgactgt gttagcctgg aagtgcttgt cccaaccttg tttctgagca tgaacgcccg
     2281 caagccaaca tgttagttga agcatcaggg cgattagcag catgatatca aaacgctctg
     2341 agctgctcgt tcggctatgg cgtaggccta gtccgtaggc aggacttttc aagtctcgga
     2401 aggtttcttc aatctgcatt cgcttcgaat agatattaac aagttgtttg ggtgttcgaa
     2461 tttcaacagg taagttagtt gctagaaccc atggctcctt tgccgacgct gagtagattt
     2521 taggtgacgg gtggtgacaa tgagtccgtg tcgagcgctg attttttcgg cctttagagc
     2581 gagatttata caatagaatt tggcatgaga ttggattgct tttagtcagc ctcttatagc
     2641 ctaaagtctt tgagtgacta gatgacatat catgtaagtt gctgataggt ttccagtttt
     2701 ccgctcctag gtctgcatat tgtacttttc ctcttactcg acttaaccag taccaaccca
     2761 gcttctcaac ggatttatac catggcactt taaagccagc atcactgaca atgagcggtg
     2821 tggtgttact cggtagaatg ctcgcaaggt cggctagaaa ttggtcatga gctttctttg
     2881 aacattgctc tgaaagcggg aacgctttct cataaagagt aacagaacga ccgtgtagtg
     2941 cgactgaagc tcgcaatacc ataagtcgtt tttgctcacg aatatcagac cagtcaacaa
     3001 gtacaatggg catcgtattg cccgaacaga taaagctagc atgccaacgg tatacagcga
     3061 gtcgctcttt gtggaggtga cgattaccta acaatcggtc gattcgtttg atgttatgtt
     3121 ttgttctcgc tttggttggc aggttacggc caagttcggt aagagtgaga gttttacagt
     3181 caagtaatgc gtggcaagcc aacgttaagc tgttgagtcg ttttaagtgt aattcggggc
     3241 agaattggta aagagagtcg tgtaaaatat cgagttcgca catcttgttg tctgattatt
     3301 gatttttcgc gaaaccattt gatcatatga caagatgtgt atccacctta acttaatgat
     3361 ttttaccaaa atcattaggg gattcatcag agcttaatac actccttaat tgaagaatcg
     3421 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
     3481 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
     3541 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
     3601 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
     3661 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
     3721 aacggatcgg cactgcgtgc gccaattctg cagacaaatg gcagtattca tccacaattt
     3781 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     3841 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
     3901 ttattacagg gacagcagag atccagtttg gttaatcgta cgaattcgaa cgctgacgtc
     3961 atcaacccgc tccaaggaat cgcgggccca gtgtcactag gcgggaacac ccagcgcgcg
     4021 tgcgccctgg caggaagatg gctgtgaggg acaggggagt ggcgccctgc aatatttgca
     4081 tgtcgctatg tgttctggga aatcaccata aacgtgaaat gtctttggat ttgggaatct
     4141 tataagttct gtatgagacc acagatcccc ggacatccag gcacacaagt tcaagagact
     4201 tgtgtgcctg gatgtccttt ttggaaagct tatcgaatta attaacccgt gtcggctcca
     4261 gatctggcct ccgcgccggg ttttggcgcc tcccgcgggc gcccccctcc tcacggcgag
     4321 cgctgccacg tcagacgaag ggcgcagcga gcgtcctgat ccttccgccc ggacgctcag
     4381 gacagcggcc cgctgctcat aagactcggc cttagaaccc cagtatcagc agaaggacat
     4441 tttaggacgg gacttgggtg actctagggc actggttttc tttccagaga gcggaacagg
     4501 cgaggaaaag tagtcccttc tcggcgattc tgcggaggga tctccgtggg gcggtgaacg
     4561 ccgatgatta tataaggacg cgccgggtgt ggcacagcta gttccgtcgc agccgggatt
     4621 tgggtcgcgg ttcttgtttg tggatcgctg tgatcgtcac ttggtgagta gcgggctgct
     4681 gggctggccg gggctttcgt ggccgccggg ccgctcggtg ggacggaagc gtgtggagag
     4741 accgccaagg gctgtagtct gggtccgcga gcaaggttgc cctgaactgg gggttggggg
     4801 gagcgcagca aaatggcggc tgttcccgag tcttgaatgg aagacgcttg tgaggcgggc
     4861 tgtgaggtcg ttgaaacaag gtggggggca tggtgggcgg caagaaccca aggtcttgag
     4921 gccttcgcta atgcgggaaa gctcttattc gggtgagatg ggctggggca ccatctgggg
     4981 accctgacgt gaagtttgtc actgactgga gaactcggtt tgtcgtctgt tgcgggggcg
     5041 gcagttatgg cggtgccgtt gggcagtgca cccgtacctt tgggagcgcg cgccctcgtc
     5101 gtgtcgtgac gtcacccgtt ctgttggctt ataatgcagg gtggggccac ctgccggtag
     5161 gtgtgcggta ggcttttctc cgtcgcagga cgcagggttc gggcctaggg taggctctcc
     5221 tgaatcgaca ggcgccggac ctctggtgag gggagggata agtgaggcgt cagtttcttt
     5281 ggtcggtttt atgtacctat cttcttaagt agctgaagct ccggttttga actatgcgct
     5341 cggggttggc gagtgtgttt tgtgaagttt tttaggcacc ttttgaaatg taatcatttg
     5401 ggtcaatatg taattttcag tgttagacta gtaaattgtc cgctaaattc tggccgtttt
     5461 tggctttttt gttagacgaa gcttgggctg caggtcgact ctagaccacc atggattgcc
     5521 tgtgcattgt aactacaaag aaataccggt atcaggacga agaggtgctg ccttcagagc
     5581 ggatttcccc gcaagtccca aatgaggtgc tgggtccgga gctggttcac gtctcggaga
     5641 agagcctgtc agagattgag aatgtccacg ggtttgtctc tcactctcat atctcaccca
     5701 taaaggcaaa tcctcctcct gtgctggtca acacagacag cttggagaca ccaacttatg
     5761 ttaatggtac tgatgcagat tatgaatatg aggaaatcac acttgaaagg ggaaattcag
     5821 gccttggttt cagcattgca ggaggtacag acaacccaca cattggagat gactcaagta
     5881 ttttcatcac caaaattatc acgggaggag cagctgccca ggatggaaga ttgcgggtaa
     5941 atgactgtat actgagagta aatgaagcag atgttcgtga tgtaacccac agcaaagcag
     6001 ttgaagcatt gaaagaagct ggatcgatag tgcgattata tgtgaaaagg cggaagccag
     6061 cctcagaaaa aatcatggaa ataaaactta ttaaaggccc taaaggtctt gggttcagca
     6121 ttgctggagg tgttggaaac cagcacattc ctggggataa cagcatctat gtaaccaaaa
     6181 taattgaagg aggtgcagca cacaaggacg gcaaacttca gattggagac aagcttctag
     6241 cagtgaacag tgtctgttta gaagaagtta ctcatgaaga agcagtgact gccttaaaga
     6301 atacatctga ttttgtttat ttgaaagtgg caaaaccaac aagtatgtat ataaatgatg
     6361 gctatgcacc acccgacatc actaattctt cttctcagtc tgttgacaac catgttagcc
     6421 cgtcttcata cttgggccag actccagcgt caccagccag atactcaccc atttctaaag
     6481 cagtgcttgg agatgatgag atcactaggg aacctagaaa agttgttctt catcgtggct
     6541 caacgggact tggtttcaac attgtgggag gcgaagatgg agaagggatt tttatctctt
     6601 tcatccttgc tgggggacct gctgacctaa gcggagagct cagaaaagga gatcgcatta
     6661 tatcggtaaa cagtgttgac ctcagagctg caagtcatga acaagcagct gccgcattaa
     6721 aaaacgcagg ccaagctgtc actattgtcg cacagtatcg gcctgaagag tacagtcgtt
     6781 ttgaagctaa aattcacgac ttgcgggagc agatgatgaa cagcagtgtc agctcagggt
     6841 cagggtctct ccgaaccagc caaaagcggt ccctctatgt cagagccctt tttgattacg
     6901 acaagactaa agacagtggc cttcccagtc aagggttgaa cttcaaattc ggagatattc
     6961 tccatgttat caatgcttct gatgacgagt ggtggcaggc cagacaggtg accccagatg
     7021 gtgagagtga cgaagttgga gtaattccta gtaaacgcag agttgagaag aaagaacgag
     7081 cccgattaaa aacagtcaaa ttcaattcta aaacaagagg agataaaggg tcattcaatg
     7141 acaagcgtaa aaagaacctc ttttcccgaa aatttccctt ctacaagaac aaggaccaga
     7201 gtgagcagga aacaagtgat gctgaccagc acgtaacttc taatgccagc gatagcgaaa
     7261 gtagttaccg tggtcaagaa gaatatgttt tatcgtatga gccggtgaat cagcaagaag
     7321 ttaattatac ccgaccagtc atcatattag gacctatgaa agacagagta aatgatgact
     7381 taatctcaga atttcctgac aaatttggat cctgtgtccc tcatacaact agaccaaagc
     7441 gtgactatga ggtggatgga cgagattacc attttgtgac ttcaagagaa cagatggaga
     7501 aagatatcca ggagcataaa ttcattgaag ctggccagta taacaaccat ctatacggaa
     7561 cgagtgtcca atctgtgcga gcagtagcag agaagggcaa gcattgtatc cttgatgtgt
     7621 ctggaaatgc catcaagagg ttgcagattg cacagctcta tccaatatct atttttatta
     7681 aacccaaatc catggaaaat atcatggaaa tgaataaacg cctaacagac gaacaggcca
     7741 gaaaaacgtt tgagagagca gtgagactgg agcaggagtt cactgagcat ttcacagcta
     7801 ttgtccaggg agacacgctg gaggacattt acaatcaagt gaagcagatc atagaagagc
     7861 agtctggtcc ttacatctgg gtcccagcaa aagaaaaatt aggatccggt ggatcaggag
     7921 gttcaggtgg aagatccatc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
     7981 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     8041 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     8101 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
     8161 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     8221 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     8281 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     8341 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     8401 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     8461 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     8521 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
     8581 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     8641 tcggcatgga cgagctgtac aagtaagcta gctcgagcta gaattaattc gatatcaagc
     8701 ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     8761 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     8821 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     8881 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     8941 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     9001 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     9061 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     9121 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     9181 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     9241 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc
     9301 gataccgtcg agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca
     9361 atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac
     9421 ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     9481 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc
     9541 tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag
     9601 ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagcaagaga
     9661 aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg agcctgcatg
     9721 ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc ctagcatttc
     9781 atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta gaccagatct
     9841 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     9901 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     9961 tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac ccgctgatca
    10021 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
    10081 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
    10141 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
    10201 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
    10261 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
    10321 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
    10381 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
    10441 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
    10501 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
    10561 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
    10621 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
    10681 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
    10741 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
    10801 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
    10861 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
    10921 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
    10981 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
    11041 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
    11101 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata
    11161 cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc
    11221 gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt
    11281 cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt
    11341 ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga
    11401 gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc
    11461 catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg
    11521 caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc
    11581 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
    11641 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
    11701 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
    11761 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
    11821 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
    11881 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
    11941 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
    12001 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
    12061 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
    12121 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
    12181 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
    12241 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
    12301 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
    12361 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
    12421 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
    12481 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
    12541 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
    12601 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
    12661 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
    12721 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
    12781 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
    12841 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
    12901 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
    12961 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
    13021 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
    13081 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
    13141 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
    13201 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
    13261 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
    13321 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
    13381 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
    13441 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    13501 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    13561 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    13621 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    13681 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    13741 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    13801 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    13861 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    13921 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    13981 gcgcacattt ccccgaaaag tgccacctga c
//
