LOCUS       sequence-20192-e         749 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Brent AE, Tabin CJ
  TITLE     FGF acts directly on the somitic tendon progenitors through the Ets 
            transcription factors Pea3 and Erm to regulate scleraxis expression.
  JOURNAL   Development. 2004 Aug . 131(16):3885-96.
  PUBMED    15253939
REFERENCE   2  (bases 1 to 749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 2004 Aug . 131(16):3885-96."
COMMENT     Sequence Label: pBS cFgfr2 (CT#221)
FEATURES             Location/Qualifiers
     source          1..749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     10..26
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10..26
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(540..556)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(541..557)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(586..606)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(586..604)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(625..641)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(625..641)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(638..660)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    649..665
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(673..679,680..697,698..703))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    718..739
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ggcggccgct ctagaactag tggatccccc gggctgcagg aattcgattg gagtctcatc
       61 atggagagcg tagtcccatc cgacaaagga aattacacgt gcatcgtgga aaaccagtat
      121 ggctccatca accacactta ccatctcgat gttgtcgagc gatcaccgca caggcccatc
      181 ctccaggctg gccttccagc aaacgcctcg gctgtagtcg gaggtgatgt cgagtttgtc
      241 tgcaaagtct acagtgatgc tcaaccccac attcagtgga taaaacacgt agagaggaat
      301 ggcagtaaat acggaccaga tggactgcct taccttcagg ttttaaaggc tgccggtgtt
      361 aacactacgg acaaagaaat tgaggttctc tatatacgga atgtaacttt tgaggatgct
      421 ggggagtata catgcttggc gggtaattct attgggatat cctttcacac tgcatggttg
      481 acagttctgc cagctcctga aaaggaaaag ggatttccca catctccaat caagcttatc
      541 gataccgtcg acctcgaggg ggggcccggt acccagcttt tgttcccttt agtgagggtt
      601 aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
      661 cacaattcca cacaacatac gagccgggag cataaagtgt aaagcctggg gtgcctaatg
      721 agtgagctaa ctcacattaa ttgncnttg
//
